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Connect to C-DiGit® Blot Scanner
11
If there is only one C-DiGit Scanner detected by the computer, it will connect
automatically; if there are multiple C-DiGit Scanners detected, you will have to
choose the desired C-DiGit Blot Scanner from a list of available instruments
(below, listed by serial number).
You can find the serial number
on a label on the underside of
the instrument.
When a connection is established, the green indicator light will illuminate on
the instrument front panel, and the Status panel in the Image Studio
dow will display 'Ready'.
NOTE: C-DiGit Blot Scanner uses IPv6 and IPv4 Link-Local Addressing to auto-
matically create an IP network without any user configuration or additional
servers. If you experience connection problems, be sure that IPv6 is enabled
on the computer.
Expose C-DiGit Blot Scanner Target
12
Open the Target envelope and expose the
side of the Target with the gray back-
ground to room light for 30 seconds. Place
the Target face down (gray background)
on the C-DiGit Blot Scanner scanning sur-
Expose this side
face.
Acquire Target Image
13
Make sure that Sensitivity is set to Standard (
Standard, click on the Sensitivity button and choose Standard from the pull-
down menu. Image acquisition using Standard sensitivity takes approximately
6 minutes to complete.
Click the Start button (
) on the Acquire tab ribbon.
Image acquisition will start, and the target image will be displayed as a series of
six images of various brightness/contrasts (Step 14).
Adjust Target Image
14
Verify that your target image is similar to that shown below. If it matches, your
C-DiGit Scanner is functioning correctly.
The highlighted icons at
right can also be found in
the rightmost pane of the
main Image Studio window,
and will open the Chooser
(right) and Adjust Display
(below) dialogs.
™
win-
Click on the best image; that image will open in a new window (below), allow-
ing for further adjustment, if desired. Click on Signal, Background, or Midtones
to adjust the brightness for these channels. Click Done.
1. Expose to room
light for 30 seconds
2. Place exposed
side down
Click the other ribbon tabs (above) for additional features, including:
Image - Adjust the image appearance, crop, rotate, view image array.
Analysis - Draw shapes to quantify data.
Annotation - Add text annotations to image.
Lab Book - Create a .pdf file containing images, data tables, and charts.
). If Sensitivity is not set to
Click on the Shapes and/or Images tabs at the bottom of the window to view:
Shapes - Table of quantification data for selected image.
Images - Table of acquired images, settings, and comments.
Congratulations!
You are now ready to image your own sample. Additional resources can be
found in the C-DiGit Blot Scanner User Guide, on the Resource CD.
www.licor.com/cdigit
LI-COR, C-DiGit and Image Studio are trademarks or registered trademarks of LI-COR, Inc.
Mac and OS X are trademarks of Apple Inc. All other trademarks belong to their
respective owners.
Doc # 984-19132 05/20
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