Imaging Workflow For Fluorescent Applications; Imaging Workflow For Nucleic Acid Gel - Azure Biosystems Azure 200 User Manual

13. Select
14. All images will be displayed in the Gallery. The Gallery can be reached by clicking
4.1 for instructions on using the Gallery.
15. Select

3.4 Imaging Workflow for Fluorescent Applications

Use this for samples with fluorescent signals in up to four channels (red, green, blue, and grayscale) for
multiplex imaging.
1. Remove the black blot tray and place blot in the center of the tray. Then return tray to
imaging chamber.
2. Close the Azure Imaging System door.
3. Select Fluorescent Blots from the home screen.
4. Select dyes in between one and four channels: red, green, blue, and grayscale.
5. Pick the name of the dye you are imaging. Note, if your dye does not appear on the list, contact
Azure Technical Support.
6. Select Auto Scan to produce an image with default settings. This is best to use when you don't know
what you expect to see on the blot. See Section 3.2 for additional details.
7. Select Manually Image to customize the settings.
8. Sensitivity – see Section 3.2 for details.
9. Exposure – see Section 3.2 for details
10. If you are interested in repositioning your sample before you take the image, use Live Mode.
See Section 3.2 for details.
11. Select
12. The images will be displayed in the Gallery. The Gallery can be reached by clicking the tile icon. See
Section 4.1 for instructions on using the Gallery.
13. Select

3.5 Imaging Workflow for Nucleic Acid Gel

Use this for nucleic acid blotting samples in up to four channels (red, green, blue, and grayscale).
1. Open door and place blot directly on trans UV illuminator.
2. Place Interlock Switch Override Key in safety interlock in order to enable UV light. (See Step 10 below
for further details if you're using this method.) Note: always wear safety goggles when utilizing
override switch.
3. Close the Azure Imaging System door.
4. Select Nucleic Acid Gel from the home screen.
5. Select dyes in between one and four channels: red, green, blue, and grayscale.
6. Pick the name of the dye you are imaging. Note, if your dye does not appear on the list, contact
Azure Technical Support.
7. Select Auto Scan to produce an image with default settings. This is best to use when you don't know
what you expect to see on the blot. See Section 3.2 for additional details.
8. Select Manually Image to customize the settings.
Azure Imaging System User Manual
to capture the image.
to go back one screen.
to capture the image.
to go back one screen.
. See Section
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