Azure Biosystems Azure Cielo Dx AIQ314 User Manual
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Azure Biosystems Azure Cielo Dx AIQ314 User Manual

Real-time pcr systems
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Azure Cielo
Dx
™
Real-Time PCR Systems
User Manual
Part Numbers AIQ314 and AIQ315
UM-0008 R3

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Summary of Contents for Azure Biosystems Azure Cielo Dx AIQ314

  • Page 1 Azure Cielo ™ Real-Time PCR Systems User Manual Part Numbers AIQ314 and AIQ315 UM-0008 R3...
  • Page 2 Safety and Regulatory Compliance Important Safety Information Please read these instructions before operating the Azure Cielo Dx system. Electrical Safety Precautions Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live circuit, fixture, receptacle, or switch. Safety rules you should follow whenever working with any electrical appliance include: •...
  • Page 3 If any defect occurs in the instrument during this warranty period, Azure Biosystems, Inc. will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: •...
  • Page 4 Contact Azure Biosystems, Inc. 6747 Sierra Court, Suite A-B • Dublin, CA 94568 • USA info@azurebiosystems.com • (925) 307-7127 • Fax (925) 905-1816 Azure Cielo Dx Real-Time PCR Systems User Manual...

  • Page 5: Table Of Contents

    Table of Contents Introduction Azure Cielo Dx Specifications Plates and Tube Compatibility Contacting Technical Support Installation and Setup System Placement System Overview Connect Azure Cielo Dx Real-Time PCR system to Internet Network Connect Azure Cielo Dx Real-Time PCR system to PC via Azure Cielo Dx Manager software Device Software Overview The Home Screen...

  • Page 6: Introduction

    Introduction The Azure Cielo Dx Real-Time PCR instrument is a high performance tool compatible with a wide variety of reagents for qPCR. The family of instruments includes the Azure Cielo 3 Dx and the Azure Cielo 6 Dx. 1.1—Azure Cielo Dx Specifications The Azure Cielo Dx instruments have the following specifications.

  • Page 7: Plates And Tube Compatibility

    Plates and Tube Compatibility — Azure Cielo Dx Real-Time PCR system is compatible with: A. Low-profile Plates • 96-well, Either Non-skirted or Semi-Skirted • Low-profile 96-well Plates: These types of plates have wells of approximate height 15.5 mm (depending on manufacturer). These are modern designs that have the potential to reduce condensate formation and offers the advantage for capturing light in fluorescence assays, low-volume reactions, and fast PCR.

  • Page 8: Installation And Setup

    Installation and Setup 2.1—System Placement WARNING: Excessive Weight Hazard – Please use two or more people to lift the system. Failure to do so can result in system damage and personal injury. As with all electrical instruments, the Azure Cielo Dx should be located away from water, solvents, or corrosive materials, on a flat and stable surface with adequate clearance on all sides.

  • Page 9: Connect Azure Cielo Dx Real-Time Pcr System To Internet Network

    Connect Azure Cielo Dx Real-Time PCR system to Internet Network — A. Wi-Fi 1. Go to the DEVICE SETTINGS. 2. Select Wi-Fi, under the NETWORK settings on the left edge of the screen. 3. Make sure that the WiFi is turned on. 4.

  • Page 10: Device Software Overview

    Device Software Overview The Azure Cielo Dx Icon based software allows you program and run protocols with a simple user interface. 3.1—The Home Screen Figure 4. The Home Screen. Button Button Name Function Set up a new run or running an existing protocol – Experiment Setup see Section 3.2 Data Manager...

  • Page 11: Protocol Manager

    3.2.1—Protocol Manager Figure 5. PROTOCOL MANAGER screen under Experiment Setup. Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connection Sort Sort in ascending or descending order • Add User • Add Protocol • Delete User Delete •...
  • Page 12 A. Tap button next to Users or Protocol and Date created to sort users and/or experiments in ascending or descending order. B. Add New User: • button to add a new user. • Tap on ‘Add User’ to add a new user. Enter the new user name using the system keyboard.

  • Page 13: Change Protocol Properties

    D. Delete exisiting user or protocol. • Tap on the User or Protocol to be deleted. • icon to delete the selected User or Protocol. Note: Deleting user will delete all existing protocol and data stored under that user. • The system will prompt you to confirm the deletion.
  • Page 14 Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connection Tap the rectangular text box to assign a name (or rename) Protocol Name the selected protocol • Select qPCR reaction volume Reaction Volume • Select OTHER button to customize reaction volume Lid Temperature Select lid temperature...

  • Page 15: Well Selection Screen

    3.2.3—Well Selection Screen • A screen with a diagram of 96 well PCR plate appear on the screen. By default, all wells are selected (in BLUE). • To deselect wells, tap on the well to clear selection. Wells are deselected in sets of two columns.

  • Page 16: Run Setup

    • A pop-up screen with an empty column appear allows user to input email address. • Input email ID and click OK. • Proceed with subsequent Experiment Setup and run protocol. • Email notification with results attached will be sent when the protocol run is successfully completed.
  • Page 17 Button Button Name Function Temperature Tap to customize reaction temperature Time Tap to customize time in the step Tap to assign total cycle number and the step number to Cycle Setting repeat every time after the last step of the cycle Tap on this icon to select the step after which a new step Add a step must be inserted...
  • Page 18 Time settings: • Tap on the time display button for each cycle to change the time. Enter the time using the key pad on the screen. Example: To set the time to 2 minutes, Tap ‘Min’ on the screen and enter the number 2 using the key pad on the screen.
  • Page 19 Advance settings on camera and/or gradient PCR: A. Camera: • By default, the camera is disabled (LIGHT GRAY ). Tap to enable the camera for the desired steps, tap on settings . Tap on the switch button next to CAMERA to turn the camera ON.
  • Page 20 Example: In the following image, gradient is ON for Step 2 and 3 and OFF for Step 1 and 4. Gradient can be turned on for all steps, individually. Melt Curve Advance Settings: • Tap the switch to turn on Melt Curve •...

  • Page 21: Run Protocol

    Figure 21. The screen display after the user tap RUN icon button. 3.2.6—Run Protocol Figure 22. The screen display after qPCR run finished. Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connection Stop Stop the run Pause Pause the run Continue the run after pause...
  • Page 22 Button Button Name Function Export Tap to export data file to USB drive Tap on this icon to select the step after which a new step Back must be inserted Table 5. Icons and buttons in RUN screen of a selected protocol. •...

  • Page 23: Data Manager

    3.3—Data Manager In the data manager screen you can access your run data for export to a USB. Figure 24. DATA MANAGER screen. Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connection Sort Sort in ascending or descending order •...
  • Page 24 Figure 26. Amplification Curve. Figure 27. Temperature Curve. 1. Amplification Curve • Tap the Amplification Curve image under PREVIEW window to view the Amplification curve shown as Figure 26. • Buttons with color indicating the channel or dye are displayed on the left of the Amplification Curve screen.
  • Page 25 B. Delete User • Select the user name under USERS list. • button to delete the selected user. Note: The selected user name and all associated protocols and experiment files will be deleted. • Tap OK to confirm the delete. C.
  • Page 26 E. Export Data • Tap “Email” button to export selected data files to designated email ID. Important: Make sure that: 1. Cielo Dx device is successfully connected to the Internet. To connect the Cielo Dx Device to the internet, please refer Section 2.3 (Connect Azure Cielo Dx Real-Time PCR system to internet network).

  • Page 27: Device Settings

    3.4—Device Settings In device settings screen, the user can customize certain information on the instrument, as well as look up important technical information for troubleshooting purposes. 3.4.1—General • Device Info – Within this screen, the user can see the factory set serial number. The device name is also visible, and this can be reset to the users choices.

  • Page 28: Email Server Setup

    3.4.3—Email Server Setup Important: Make sure that the Cielo Dx is successfully connected to the Internet. To connect the Cielo Dx Device to the internet, please refer Section 2.3 (Connect Azure Cielo Dx Real-Time PCR system to internet network). To enable email features on Azure Cielo Dx Real-Time PCR system, Cielo Dx must be connected to the internet network, and established email server with the following instructions: Go to Device Settings Go to Network >...
  • Page 29 Email Provider IMAP Settings POP Settings SMTP Settings Server: Server: Server: imap-mail.outlook.com pop-mail.outlook.com smtp-mail.outlook.com Port: 993 Port: 995 Port: 587 Encryption: SSL/TLS Encryption: SSL/TLS Encryption: STARTTLS Server: Server: Server: outlook.office365.com outlook.office365.com smtp.office365.com Microsoft 365 Port: 993 Port: 995 Port: 587 Encryption: SSL/TLS Encryption: SSL/TLS Encryption: SSL/TLS...

  • Page 30: Dye Manager

    3.4.4—Dye Manager This tool is designed for users who need to add a custom dye to their system. Settings include how to set up the dye plate for calibration. • Dye List – By default the channels are indicated with dye name and the excitation/emission wavelength in light blue color.

  • Page 31: Faqs And Troubleshooting

    FAQs and Troubleshooting The Azure Cielo Dx Icon based software allows you program and run protocols with a simple user interface. 4.1—FAQs Question Answer Best with 2-fold dilutions, minimum 20μl of reaction volume and What is the best practice for new or custom assign the stock dye reagent as the highest concentration in H1 dye calibration? and H2 wells.
  • Page 32 • info@azurebiosystems.com Copyright © 2021 Azure Biosystems. All rights reserved. The Azure Biosystems logo, Azure Biosystems , and Cielo are trademarks ® ™ of the Company. All other trademarks, service marks and trade names appearing in this brochure are the property of their respective owners.

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