Related Manuals for Halma Ocean Optics QE65 Pro
Summary of Contents for Halma Ocean Optics QE65 Pro
- Page 1 QE65 Pro Scientific-grade Spectrometer Installation and Operation Manual For Products: QE65Pro Document: 220-12200-000-02-201603 000-00000-000-02-A...
- Page 2 AMERICAS & WORLD HEADQUARTERS Phone: +1 727-733-2447 Ocean Optics, Inc. Fax: +1 727-733-3962 830 Douglas Ave. Dunedin, FL 34698 Sales: info@oceanoptics.com Orders: orders@oceanoptics.com Support: techsupport@oceanoptics.com Manufacturing & Logistics 4301 Metric Dr. Winter Park, FL 32792 EUROPE, MIDDLE EAST & AFRICA Sales &...
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Page 3: Table Of Contents
Table of Contents About This Manual ......................iii Document Purpose and Intended Audience ................iii Document Summary ........................iii Product-Related Documentation ....................iii Upgrades ........................iv Chapter 1: Introduction ..............1 Product Overview ......................1 Features ........................2 System Requirements ....................3 EEPROM Utilization ........................ - Page 4 Table of Contents Remove Improperly Installed Files ..................12 Mac Operating Systems ......................12 Linux Operating Systems ......................12 Appendix A: Calibrating the Wavelength of the QE65 Pro ..... 13 Overview ........................13 About Wavelength Calibration ..................13 Calibrating the Spectrometer ..................14 Preparing for Calibration ......................
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Page 5: About This Manual
About This Manual Document Purpose and Intended Audience This document provides the user of the QE65 Pro Spectrometer with instructions for setting up, calibrating and performing experiments with their spectrometer. What’s New in this Document updates This version of the QE65 Pro Scientific-grade Spectrometer Installation and Operation Manual the specifications Document Summary Chapter... -
Page 6: Upgrades
About This Manual Document for… Document Location SpectraSuite software http://oceanoptics.com///wp-content/uploads/SpectraSuite.pdf HR-4 Breakout Box http://oceanoptics.com///wp-content/uploads/HR-4-Breakout-Box.pdf External triggering for http://oceanoptics.com///wp-content/uploads/External-Triggering-Options.pdf firmware versions below 3.0 External triggering for http://oceanoptics.com///wp-content/uploads/External-Triggering- firmware versions 3.0 Options_Firmware3.0andAbove.pdf and above Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your state-of-the-art products and how they function, located at: http://oceanoptics.com/glossary/. - Page 7 3: Troubleshooting WARNING This is a Class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures. FCC COMPLIANCE This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
- Page 8 About This Manual 220-12200-000-02-201603...
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Page 9: Chapter 1: Introduction
UV and shortwave NIR regions. The Ocean Optics QE65 Pro Spectrometer produces lower stray light and provides superior dynamic range and high sensitivity for low-light level applications such as fluorescence, Raman analysis and DNA sequencing. -
Page 10: Features
1: Introduction The QE65 Pro’s onboard module has 10 user-programmable digital I/O lines for interfacing to other equipment; and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. -
Page 11: System Requirements
1: Introduction EEPROM storage for Wavelength Calibration Coefficients Linearity Correction Coefficients Absolute Irradiance Calibration (optional) Plug-n-play interface for PC applications 30-pin connector for interfacing to external products System Requirements You can use the QE65 Pro’s USB connectivity with any PC that meets the following requirements: ... -
Page 12: How Sampling Works
1: Introduction Sampling System Overview How Sampling Works Ocean Optics components function in a sampling system as follows: 1. The user stores reference and dark measurements to correct for instrument response variables. 2. The light transmits through an optical fiber to the sample. 3. -
Page 13: Shipment Components
1: Introduction Shipment Components QE65 Pro Spectrometer + 5VDC power supply The following information and documentation also ships with the QE65 Pro Spectrometer: Packing List The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice arrives separately). - Page 14 1: Introduction 220-12200-000-02-201603...
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Page 15: Chapter 2: Installing The Qe65 Pro
Chapter 2 Installing the QE65 Pro Overview You must install the operating software application prior to connecting the QE65 Pro Spectrometer to the computer. The Ocean Optics spectrometer operating software installs the drivers required for the QE65 Pro spectrometer installation. If you do not install the software first, the system will not properly recognize the QE65 Pro. -
Page 16: Serial Port Mode
2: Installing the QE65 Pro If you installed the spectrometer operating software prior to connecting the QE65 Pro, the software installs the QE65 Pro drivers. If the drivers do not successfully install (or if you connected the QE65 Pro to the computer before installing the software), consult Chapter 3: Troubleshooting. Serial Port Mode To use the serial port capacity of the QE65 Pro Spectrometer, the PC must be running a 32-bit version of the Windows operating system (or Windows CE 2.11 or higher for handheld PCs). - Page 17 2: Installing the QE65 Pro For QE65 Pro firmware version 3.0 and above, see the External Triggering Options document located at http://oceanoptics.com///wp-content/uploads/External-Triggering-Options_Firmware3.0andAbove.pdf. The triggering document contains instructions for configuring External Triggering options for the QE65 Pro. Note Only the external software triggering option is available when using a handheld computer.
- Page 18 2: Installing the QE65 Pro 220-12200-000-02-201603...
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Page 19: Chapter 3: Troubleshooting
Software Installation Windows Operating Systems If you connected your Ocean Optics QE65 Pro device to the computer prior to installing your spectrometer operating software application (OceanView) on a Windows platform, you may encounter installation issues that you must correct before your Ocean Optics device will operate properly. -
Page 20: Remove Improperly Installed Files
3: Troubleshooting 3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown Device listing and select the Uninstall or Remove option. 4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown Device. -
Page 21: Appendix A: Calibrating The Wavelength Of The Qe65 Pro
Appendix A Calibrating the Wavelength of the QE65 Pro Overview This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. -
Page 22: Calibrating The Spectrometer
A: Calibrating the Wavelength of the QE65 Pro Calibrating the Spectrometer Preparing for Calibration To recalibrate the wavelength of your spectrometer, you need the following components: A light source capable of producing spectral lines Note Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an HG-1, you need a light source that produces several (at least 4-6) spectral lines in the wavelength region of your spectrometer. - Page 23 A: Calibrating the Wavelength of the QE65 Pro Independent Values Computed Dependent from the Regression Variable Variables Output Predicted True Wavelength (nm) Pixel # Pixel # Pixel # Difference Wavelength 253.65 30625 5359375 253.56 0.09 296.73 87616 25934336 296.72 0.01 302.15 97344 30371328...
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Page 24: Saving The New Calibration Coefficients: Usb Mode
A: Calibrating the Wavelength of the QE65 Pro 7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at the value for R squared. It should be very close to 1. If not, you have most likely assigned one of your wavelengths incorrectly. -
Page 25: Appendix B: Specifications
Appendix B Specifications Overview This appendix contains information on spectrometer operation, specifications, and system compatibility. It also includes accessory connector pinout diagrams and pin-specific information. How the QE65 Pro Works Below is a diagram of how light moves through the optical bench of a QE65 Pro Spectrometer. The optical bench has no moving parts that can wear or break;... -
Page 26: Qe65 Pro Components Table
B: Specifications QE65 Pro Components Table Ocean Optics permanently secures all components in the QE65 Pro at the time of manufacture. Only Ocean Optics technicians can replace interchangeable components, where noted. Item Name Description Secures the input fiber to the spectrometer. Light from the input fiber enters the Connector optical bench through this connector. -
Page 27: Qe65 Pro Specifications
B: Specifications QE65 Pro Specifications The following sections provide specification information for the CCD detector in the QE65 Pro, as well as the QE65 Pro Spectrometer itself. CCD Detector Specifications Specification Value Detector Hamamatsu S7031-1006 Low Etaloning Detector range 200-1100 nm Pixels 1024 x 58 (1044 x 64 total pixels) 24 μm... -
Page 28: System Compatibility
B: Specifications Specification Value 4.5 – 5.5 V Supply Voltage Power-up Time < 5 seconds Signal-to-noise ratio 1000:1 (single acquisition) Gratings 21 gratings available Entrance aperture 5, 10, 25, 50, 100 or 200 µm wide slits OFLV-QE (200-950 nm); OFLV-QE-250 (250-1000 nm); OFLV-QE-300 OFLV filters (300-1050 nm);... -
Page 29: 30-Pin Accessory Connector Pinout Diagram
B: Specifications Location of QE65 Pro 30-Pin Accessory Connector 30-Pin Accessory Connector Pinout Diagram When facing the 30-pin Accessory Connector on the front of the vertical wall of the QE65 Pro, pin numbering is as follows: Port 30-Pin Accessory Connector Pinout Diagram 30-Pin Accessory Connector –... - Page 30 B: Specifications Function Input/Output Description Ground Input/Output Ground C SCL Input/Output C clock signal for communication to other I C peripherals General purpose software-programmable, digital input/output GPIO (0) Input/Output (channel number) C SDA Input/Output C data signal for communication to other I C peripherals General purpose software-programmable, digital input/output GPIO (1)
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Page 31: 30-Pin J2 Accessory Connector - Part Numbers
B: Specifications Function Input/Output Description General purpose software-programmable, digital input/output GPIO (6) Input/Output (channel number) Reserved Reserved Lamp TTL signal driven Active HIGH when the Lamp Enable command Output Enable is sent to the spectrometer General purpose software-programmable, digital input/output GPIO (7) Input/Output (channel number) -
Page 32: Qe65 Pro 15-Pin Accessory Cable Pinout
B: Specifications QE65 Pro 15-Pin Accessory Cable Pinout Pin # Description Pin # Description Single_strobe GPIO-9 ContStrobe GND_SIGNAL V5_SW ExtTrigIn ExtTrigIn LampEnable GPIO-8 A_IN A_OUT GPIO-7 ExtTrigIn 220-12200-000-02-201603... -
Page 33: Index
Index 15-pin accessory cable Detector Collection Lens, 18 pinouts, 24 document audience, iii purpose, iii summary, iii 30-pin accessory connector diagram, 21 part numbers, 23 pin definitions, 21 EEPROM, 3 External Triggering, 8 accessories, 5, 8 Accessories, 4 filter, 18 accessory connector focusing mirror, 18 pinout, 20... - Page 34 B: Specifications memory chip, 3 TE Cooler, 1, 2, 7, 19 mirror, 18 Triggering, 8 troubleshooting Linux systems, 12 Mac systems, 12 Troubleshooting, 11 OceanView, 3 Options Interface, 4 upgrades, iv USB mode, 7 USB-ADP-PC, 4 packing list, 5 USB-CBL-1, 4 power supply (external), 2, 3, 4, 5, 7, 19, 20 product-related documentation, iii warranty, iv...