Related Manuals for Peak C-7100 Series
Summary of Contents for Peak C-7100 Series
- Page 1 C-7100 Series User Manual PEAK INSTRUMENTS INC. C-7100 Series Spectrophotometer Operating Manual and Maintenance Guide PEAK INSTRUMENTS INC. Version 1701...
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Page 2: Table Of Contents
C-7100 Series User Manual PEAK INSTRUMENTS INC. CONTENT I. Spectrophotometer Overview ...........................1 1. Basic operating principle ............................1 2. Performance Indicators Definitions ..........................3 3. Application ................................4 4. Using conditions ................................4 II. Product features and installation 1. Features ..................................5 2. Performance indicators ............................6 3. - Page 3 C-7100 Series User Manual PEAK INSTRUMENTS INC. VII. Time Scan (Kinetics)……………………………………………………………………………………………………………….………………………..…20 1. Function description ..............................20 2. Time scanning parameters set ..........................20 3. Data test .................................21 4. Data list ..................................21 5. Slope calculation..............................22 VIII. Wavelength scanning ............................22 1. Function description ..............................22 2.
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Page 4: Spectrophotometer Overview
The smaller the energy absorption, the wavelength of light corresponding to the absorption peak at a longer wavelength. When the infrared absorbent is formed in the infrared absorption spectrum, if the energy absorption is larger, the shorter the wavelength corresponding to the absorption peak at a shorter wavelength, when generating the ultraviolet absorption spectra of absorption in the ultraviolet region. - Page 5 (in the absorption spectrum of the peak of the more significant ) I. Impact of non-parallel incident, one of the prerequisites ear than the law is the use of a parallel incident beam to ensure that all beams through the same thickness of the absorbing medium, when a large deviation from parallelism when the incident beam, obviously lead to deviations from Beer's law.
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Page 6: Performance Indicators Definitions
It reflects the wavelength drive mechanism and the stability of the whole instrument; 5) Spectral bandwidth (sensitivity, resolution): refers to a peak spectral bands when the slit on the detector detected through a monochromator energy half-width, expressed in nm wavelength, from another perspective to... -
Page 7: Application
C-7100 Series User Manual PEAK INSTRUMENTS INC. 6) Stray light: wavelength of stray light is irradiated onto the non-selection signal generated by the detector. It is an important source of photometric analysis of errors, stray light limit the accuracy of the General Assembly high concentration solution analysis. -
Page 8: Product Features And Installation
C-7100 Series User Manual PEAK INSTRUMENTS INC. vibrations; nearby fan of the instrument should be left enough space to exhaust smoothly. Instrument is best to use a single power outlet, power should ensure good grounding. Doing so may result in equipment not working properly. -
Page 9: Performance Indicators
C-7100 Series User Manual PEAK INSTRUMENTS INC. 2. Performance Indicator Model C-7100 C-7100S C-7100A Display 7 inch TFT Keyboard Control Silicone Buttons Single Beam Optical System Holographic grating, 1200 lines/mm Slit Width 1 nm 0.5,1,2,4 nm Wavelength 190 - 1100nm... -
Page 10: Main Functions
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3. Main Functions... -
Page 11: Structure Introduction
C-7100 Series User Manual PEAK INSTRUMENTS INC. 4.Structure introduction Overall structure consists of three parts: optical system, power system and micro-computer system. 1) Top view: 2) Bottom view:... - Page 12 C-7100 Series User Manual PEAK INSTRUMENTS INC. 3) Light path diagram 4) Important part of the instrument A. Power Board: The 220V50Hz/110V60Hz power conversion and regulation outside access to the necessary equipment:-11.5V (tungsten lamp power), +12 V (fan power, electrical power), +5 V (computer system power), + /- 15V (signal board power supply), deuterium lamp filament preheating and lit power deuterium lamp is lit up and the power breakdown.
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Page 13: Instrument Installation
C-7100 Series User Manual PEAK INSTRUMENTS INC. accuracy, the slits there are two general representation of the width, the actual width of the slit of a knife-edge between the two expressed (in mm), the other to represent the bandwidth of spectral bands (in nm). -
Page 14: Key Definitions And Basic Operation
C-7100 Series User Manual PEAK INSTRUMENTS INC. III. Key definitions and basic operation 1. Button panel schematic diagram 2. Button function description Button name Button functional description 【MENU】 Menu key under each function 【PRINT】 Print output button 【SAVE】 File storage button 【LOAD】... -
Page 15: Basic Operation
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3. Basic operation 1) How to adjust the blank In any test interface, put the cuvette containing the reference solution into cuvette slot, and pull it into light path., 【Zero】 press key to adjust the blank. -
Page 16: Printer Check
If the argument is wrong wavelength, then start looking for a deuterium lamp to automatically correct the characteristic peak wavelength. Wavelength calibration result is displayed by √, the correction is not passed, then the result is ×, the buzzer alarm. -
Page 17: Photometric Measurement
C-7100 Series User Manual PEAK INSTRUMENTS INC. Note: After power on the instrument, the instrument will automatically self-test and initialization until after initialization is complete, the instrument will warm up for 20 minutes, 20 minutes warm-up time or press [ESC] to skip preheating case, the instrument being prompted preparatory work environment, which is the instrument re- calibration dark current, set the working parameters, etc., and then enter the main menu. -
Page 18: Set Wavelength
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3.Set wavelength Set the current working wavelength range 190nm-1100nm, press the up and down button to open wavelength setting window, press [0] - [9] to enter the desired wavelength, press [CLEAR] to clear the input, press [ENTER] to confirm. -
Page 19: Delete Data
C-7100 Series User Manual PEAK INSTRUMENTS INC. 6. Delete data Delete files Press [CLEAR] button, delete files prompt box will pop up, this operation will delete all the test data currently under test. Select [Yes], the file will be deleted all the data, select [No] will return the test window. -
Page 20: Open The File
C-7100 Series User Manual PEAK INSTRUMENTS INC. 8. Open the file In photometric measurement interface will be displayed by the Load button to open the window, open the window has all the photometric data file list. Select the appropriate data file and press [ENTER], the file will be read into all the test data, and then automatically enters the measurement mode. -
Page 21: Set Parameter
C-7100 Series User Manual PEAK INSTRUMENTS INC. 1. Build standard curve To use several configured standard samples, input the concentration of sample standard, collect the absorbance of standard sample, through the relationship between concentration and absorbance is calculated curve parameters, use this parameter to measure the concentration of the sample. -
Page 22: Sample Measurement
C-7100 Series User Manual PEAK INSTRUMENTS INC. Put various standard samples into cuvette holder orderly, and enter the concentration value of each sample, then press [enter] key, read the absorbance of the sample. The input is completed, the data of the standard sample is calculated automatically according to the parameters of the curve, displayed on the screen. -
Page 23: Time Scan (Kinetics)
C-7100 Series User Manual PEAK INSTRUMENTS INC. VII. Time scan (Kinetic) 1. Function description Scan time (kinetic) function is a fixed time interval to the trend in absorbance or transmittance of the test current, and displayed on the map. Select time scanning (kinetic) menu option press [ENTER]. -
Page 24: Data Test
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3. Data test Press [START] to start measuring. the current real-time map will be on the screen. 4. Data list Press [Menu] key to check the scanning data after scanning finish. -
Page 25: Slope Calculation
C-7100 Series User Manual PEAK INSTRUMENTS INC. Slope calculation After scanning, press [Menu] key to set starting time and end time for slope calculate. VIII. Wavelength scanning 1. Function description In the set wavelength range, in a certain wavelength interval to record the absorbance of the sample, and the energy transmittance value, the results are plotted in the map, which can be seen in the sample absorbance, transmittance, and the energy value trends at different wavelengths. -
Page 26: System Baseline
C-7100 Series User Manual PEAK INSTRUMENTS INC. 1) Set start wavelength and end wavelength The start wavelength and end wavelength mean the wavelength range used for scanning. 2) Set the measurement mode The measurement mode is absorbance, transmission or energy. Choose different measurement mode, you need to re-set the display to display the upper and lower limits. -
Page 27: Create A User Baseline (Corrected Blank)
C-7100 Series User Manual PEAK INSTRUMENTS INC. 4. Create a user baseline (corrected blank) Before starting the measurement, the user must establish a baseline, that is, correction of the reference sample 100% T and 0Abs. -
Page 28: Begin Testing
C-7100 Series User Manual PEAK INSTRUMENTS INC. 5. Begin testing The tested samples were placed in the light path, press [START] to start the test. 6. Peaks and troughs After the test is finished, you can choose to find peaks and valleys peaks and valleys value of test results. -
Page 29: Data List
C-7100 Series User Manual PEAK INSTRUMENTS INC. 7. Data list Press [Menu] key to see the list of test data. IX. Multi-wavelength test 1. Function description Multi-wavelength for the user is needed to test a sample while measuring the transmittance or absorbance at a wavelength setting of several functions, the user interface can be placed in this first sample obtained values for several wavelengths simultaneously, thus simplifying user operation processes. -
Page 30: Set Parameter
C-7100 Series User Manual PEAK INSTRUMENTS INC. 2. Set parameter 1) Set the number of wavelength measurement When you select a multi-wavelength test capability will be prompted to enter the number of the instrument wavelength. 2) Set test wavelength After setting the number of wavelengths measuring wavelength will enter the setup interface, this interface input wavelength values of all measured using the number keys, lost a good point [ENTER] after confirming then lose the next one, until all shall be measured wavelengths losers. -
Page 31: Protein/Nucleic Acid Measurement
C-7100 Series User Manual PEAK INSTRUMENTS INC. X. Protein/nucleic acid measurement 1. Function description Protein / nucleic acid comprising two tests: Measurement of protein and single-stranded nucleic acid testing. The curve and parameter is installed inside instrument for the two tests and users can make tests without building... -
Page 32: Set Parameter
C-7100 Series User Manual PEAK INSTRUMENTS INC. 2. Parameter selection... -
Page 33: Set Zero
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3. Set zero Put reference solution into light path after selecting mode, press [ZERO] key to set zero. 4. Test Put sample solution into light path, press [Start] key to measure. XI. System setting... -
Page 34: Dark Current Measurements
C-7100 Series User Manual PEAK INSTRUMENTS INC. 1. Dark current measurements Long time operation of instrument probably lead to drift of dark current. this function can make correction for full range of dark current. Please make sure to cover the sample chamber lid when test dark current. -
Page 35: Time And Date Settings
C-7100 Series User Manual PEAK INSTRUMENTS INC. 3. Time and date settings Set the time and date of the instrument, set the year, month, day, hour, minute, second. By the arrow keys to select the year, month, day, hour, minute, second, through the numeric keys currently selected content. Press Enter to confirm your entry, press Esc to abandon input. -
Page 36: General
C-7100 Series User Manual PEAK INSTRUMENTS INC. 5. General 6. File system... -
Page 37: Usb Storage Device
C-7100 Series User Manual PEAK INSTRUMENTS INC. 7. USB storage device 8. Restore factory settings This operation will restore all system configuration information, this operation does not affect the system baseline and data files. -
Page 38: System Information
C-7100 Series User Manual PEAK INSTRUMENTS INC. 9. System information You can view software version and hardware version information. XII. Instrument Care and Maintenance 1. Routine precautions Any instrument is concerned, the correct use is the best safeguard for equipment maintenance, with emphasis on... -
Page 39: Instrument Care And Maintenance
2) Wavelength accuracy check: Buy a calibrated holmium oxide solution in the instrument wavelength scanning interface scanning range corresponding absorption spectra, measured by the detection of reconciliation between the peak value of the calibration standard, the error is between wavelength accuracy of the instrument error, it is recommended to check once a year,... -
Page 40: Self-Test Failure
C-7100 Series User Manual PEAK INSTRUMENTS INC. 2. Self-test failure Failures Causes Solutions Opening the housing service personnel Loose electrical line filter under the guidance of re-seated Opening the housing service personnel Optocoupler line filter loose under the guidance of re-seated... -
Page 41: Light Source Replacement
C-7100 Series User Manual PEAK INSTRUMENTS INC. XIV. Light source replacement Note: To make the instrument work, choose to replace original parts! ! ! Device names mentioned in this chapter and its location, please refer to Chapter 2 Section 4 describes where to find the structure. -
Page 42: Deuterium Lamp Replacement
C-7100 Series User Manual PEAK INSTRUMENTS INC. 2. Deuterium lamp replacement 1)Turn off the instrument and unplug the power cord and disconnect the equipment on both sides of the four screws fastening the shell and the shell gently remove the left vertical against the instrument. (Note: The...