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National 131-RLED Instructions Manual page 3

Compound microscopes

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2. Assure that light is available for illuminating the specimen.
a. Your microscope has special LED illumination that is powered by 3 rechargeable AA nickel metal hydride
batteries (supplied). These batteries may be recharged, as required, using the recharger (supplied). Each set of
batteries may be recharged approximately 500 times before replacing, and each charge will provide up to 50
hours of microscope operation. The LED component (bulb) will last for up to 50,000 hours before replacement is
required.
DO NOT USE regular AA alkaline batteries. Use of other than rechargeable AA nickel metal hydride
batteries could result in batteries exploding during recharge. ONLY USE THE SUPPLIED SWITCHING
BATTERY RECHARGER WITH AUTOMATIC "TRICKLE CHARGE".
b. It is recommended that you charge the batteries before initial use and after prolonged storage as the batteries
may have discharged. Plug output cord from battery charger into DC recharging socket located on back of
microscope base. Your automatic switching recharger operates on 100 to 240 volts AC 50/60 Hz. Plug recharger
into your AC wall outlet. Battery recharger is also equipped with an automatic "trickle charge" feature, the red
LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge. After
batteries are charged, the red LED indicator lamp will turn to green and charger automatically switches to "trickle
charge". The charger can be left plugged in, but for safety reasons it is a good idea to disconnect the charger
from the AC wall outlet and the output cord from recharging socket after 12 hours. Batteries and charger may feel
warm when charging, and unplugging the recharger is a safety precaution.
Note that your microscope can be used during recharging. Simply turn "on/off" rheostat switch located on
microscope base to "on" position, rotate intensity knob until image is illuminated and proceed as follows.
c. In case of equipment malfunction, see Troubleshooting procedures located at the back of this manual.
3. Rotate coarse focus knobs to move stage down (away) from objective lens as far as possible.
4. Place specimen slide, cover slip facing up, on stage with specimen centered over condenser lens in middle of stage.
5. Rotate disc diaphragm until largest aperture is positioned beneath condenser in center of stage.
6. Turn the objective turret until the 4x (smallest) objective lens "clicks" into position in the optical path. Note that each
time you change from one objective lens to another you should turn the turret until you hear the "click", which
indicates that the lens is properly indexed in the optical path.
7. While looking through the eyepiece, rotate coarse focusing knobs until specimen comes into focus. If image does not
appear in field of view, move specimen slide slightly until image appears in field of view.
8. Adjust fine focus controls until specimen is in sharp focus.
9. Turn the disc diaphragm, observing that different apertures affect the sharpness of the image. Turn diaphragm until
sharpest possible image is obtained. Note that when turning the disc diaphragm, you should hear a "click" as each
aperture comes into proper position under the condenser lens. If aperture is not properly positioned, you will observe
shadows in the field of view when looking through the microscope.
10. Changing magnification
a. Magnification is changed by rotating objective turret until different objective lens is moved into optical path.
Always turn turret until you hear the "click", indicating that lens is properly indexed. Otherwise, you will not be
able to see anything when looking through the microscope.
b. Standard lenses provided with your microscope are a widefield 10x eyepiece. 4x. 10x, and 40x objectives. The
40x objective lens has a special spring retractable mechanism which retracts slightly if front of lens comes in
contact with the specimen slide. See chart below for specifications on objectives.
WARNING
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