KERN OKN-1, OKO-1 Version 1.0 01/2015 User instructions Metallurgical microscope Table of contents Before use ...................... 3 General notes ............................3 Notes on the electrical system ....................... 3 Storage ..............................4 Maintenance and cleaning ........................5 Nomenclature ....................6 Technical data / Features ................9 Assembly ......................
1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments.
Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down.
1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth.
4.1 Reflected light unit At first you have to unite the lamp housing and the reflected light unit on their connection points. After that the connection has to be fixated by an Allen screw, which is attached to the right-hand side of the connection point of the lamp housing. Now the slides for analyser, polariser and colour filter can be attached to the appropriate slots (see page 8).
4.4 Eyepieces You must always use eyepieces with the same magnification for both eyes. These are simply placed onto the tube connectors, once you have first removed the plastic protective caps. There is no way of fixing them. You should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures.
5 Operation 5.1 Getting started The very first step is to establish a power connection using the mains plug. You should first adjust the dimmer to a low level, so that when you look through the eyepiece for the first time, your eyes are not immediately subject to a high level of light.
5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).
5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly.
5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path.
5.6 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces.
5.7 Using oil immersion objectives The 100x objectives of the OKO-1 and OKN-1 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images.
5.8 Adjusting the Koehler illumination for transmitted light OKO-1 To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. If, as with the devices in the KERN OKO-1 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light.
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3. Adjust the height of the condenser until the image from the field diaphragm appears clearly in the field of view. For some microscopes there is a risk that you will lift the condenser up so high that it collides with the object holder. Therefore care is needed when doing this.
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6. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image. The scale divisions on the condenser can be used as a guideline. Select in accordance with the objective being used. The view in the tube without the eyepiece should look something like the illustration on the right.
5.9 Adjusting the illumination for incident light OKN-1, OKO-1 Just like the components of the transmitted illumination those ones of the incident illumination can be adjusted according to the application requirements as well. The following components are provided on this occasion:...
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Centring of the lamp When using the reflected light unit for a long time, the lamp holder possibly moves out of its centre due to the heat development. In this case the illumination is no longer efficiently adjusted. This circumstance can be identified by the user when the background brightness of the field of view has an uneven distribution.
6 Changing the bulb 6.1 Transmitted light OKO-1 You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt. Before changing the bulb the device must be switched off and unplugged.
6.2 Reflected light OKO-1, OKN-1 You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt. Before changing the bulb the device must be switched off and unplugged.
8 Using optional accessories 8.1 Polarisation unit (Transmitted light) OKO-1 The polarisation unit consists of a polariser and an analyser. Both consist of a round glass plate with an external holder ring. To mount the analyser there is a slider with a socket fitted on the front of the microscope directly below the nosepiece (see illustration on page 10), where you can simply insert the analyser (see left illustration).
8.2 Camera connection (only for trinocular version) Due to the trinocular tube, which can be optionally fitted for the OKN-1 and OKO-1 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed.
8.4 Phase-contrast units (Transmitted light) OKO-1 Fundamentally there are two different ways for you to conduct the phase-contrast procedure. A simple method and a more comprehensive method. 1. Simple phase-contrast unit This consists of a simple PH condenser, a PH objective with a specific magnification (10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a centring telescope and two green filters.
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With the surface marked “TOP” facing up, the PH slider is pushed upwards into the insertion point on the PH condenser until it clicks into the first position. At this first position, the phase ring of the PH slider is in the beam path. If the aperture setting on the PH condenser is set to “PH”, then the phase-contrast application can begin.
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The adjustment for this is carried out as follows. a. First adjust the microscope using bright field mode. b. Move the hub to the required PH position (e.g. “10x”). c. Open the aperture diaphragm on the condenser to its maximum (slider to the left).
9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set...
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Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
10 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.
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