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KERN Optics OPM-1 User Instructions

Polarisation microscope
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User instructions
Polarisation microscope
KERN
OPM-1, OPN-1, OPO-1
Version 1.0
01/2016
OPM-1_OPN-1_OPO-1-BA-e-1510
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: info@kern-sohn.com
OPM 181
OPN 182, OPN 184
OPO 183, OPO 185
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com

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  • Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Polarisation microscope KERN OPM-1, OPN-1, OPO-1 OPM 181 OPN 182, OPN 184 OPO 183, OPO 185 Version 1.0 01/2016 OPM-1_OPN-1_OPO-1-BA-e-1510...

  • Page 3: Table Of Contents

    KERN OPM-1, OPN-1, OPO-1 Version 1.0 01/2016 User instructions Polarisation microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............

  • Page 4: Before Use

    1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments.

  • Page 5: Storage

    Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down.

  • Page 6: Maintenance And Cleaning

    1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth.

  • Page 7: Nomenclature

    2 Nomenclature OPM-1_OPN-1_OPO-1-BA-e-1510...

  • Page 8: Rear View

    Rear view OPM-1_OPN-1_OPO-1-BA-e-1510...
  • Page 9 Analyser unit / Reflected light unit OPM-1_OPN-1_OPO-1-BA-e-1510...

  • Page 10: Technical Data / Features

    3 Technical data / Features Eyepieces: WF 10x / Ø 18 mm Objectives: Non-stress 4x / 10x / 20x / 40x OPM 181, OPN 182, OPN 184 Objectives: Non-stress 4x / 10x / 20x / 40x / 60x OPO 183, OPO 185 Product dimension: 500x200x500 mm Net weight:...
  • Page 11 OPM-1_OPN-1_OPO-1-BA-e-1510...
  • Page 12 OPM-1_OPN-1_OPO-1-BA-e-1510...

  • Page 13: Assembly

    4 Assembly OPM-1_OPN-1_OPO-1-BA-e-1510...

  • Page 14: Analyser Unit (+ Reflected Light Unit)

    4.1 Analyser unit (+ reflected light unit) For devices without reflected light unit (OPM-1) it is only needed to mount the analyser unit. For devices, which are equipped with a reflected light unit (OPN-1, OPO-1), the analyser unit and the main part of the reflected light unit are already firmly connected.

  • Page 15: Eyepieces

    4.5 Condenser (Swing out) / Transmitting light polariser OPM-1, OPO-1 We recommend that you use the course adjustment knob to bring the specimen stage to its uppermost position. Use the focus dial of the condenser to move the condenser holder to the central position.

  • Page 16: Pre-) Focussing

    5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).

  • Page 17: Adjusting The Interpupillary Distance

    5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly.

  • Page 18: Centre-Adjusting The Stage

    For getting ideal results the centre of the rotation axis of the table must be aligned to the centre of the optical beam path. The microscopes of the OPM-1, OPN-1 and OPO-1 series are correctly set at factory. However we recommend to regularly check before the first use and every now and then if the table is centre-adjusted.
  • Page 19 1. Bring the 10x objective into beam path. 2. Assure, that one eyepiece with scale is attached to (one of) the tube connector(s). 3. Locate an appropriate specimen slide onto the table. This slide should preferably be equipped with a micro reticule. It would be also possible to use an object which includes plenty of single dots and for which one of those dots has such a size, so that it aligns with the centre cross point of the scale, visible inside of the eyepiece(s).

  • Page 20: Adjusting The Magnification

    5.6 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path.

  • Page 21: Using Eye Cups

    5.7 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces.

  • Page 22: Adjusting The Analyser Unit

    5.8 Adjusting the analyser unit In order to be able to apply the polarisation method, besides of the bright field method, certain components need to be adjusted. Basically, therefore the correct interaction between polariser and analyser is required. The analyser is located in one of the round apertures of a certain slide, the other aperture is empty (glass plate).

  • Page 23: Adjusting The Koehler Illumination For Transmitted Light

    If, as with the devices in the KERN OPM-1 and OPO-1 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light.
  • Page 24 3. Adjust the height of the condenser until the image from the field diaphragm appears clearly in the field of view. For some microscopes there is a risk that you will lift the condenser up so high that it collides with the object holder. Therefore care is needed when doing this.
  • Page 25 6. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image. The scale divisions on the condenser can be used as a guideline. Select in accordance with the objective being used. The view in the tube without the eyepiece should look something like the illustration on the right.

  • Page 26: Adjusting The Illumination For Incident Light

    5.10 Adjusting the illumination for incident light OPN-1, OPO-1 Just like the components of the transmitted illumination those ones of the incident illumination can be adjusted according to the application requirements as well. The following components are provided on this occasion: Field diaphragm and aperture diaphragm Both of the diaphragms have the same functions like described for the adjustment of the transmitted light (see section 5.9).
  • Page 27 Centring of the lamp When using the reflected light unit for a long time, the lamp holder possibly moves out of its centre due to the heat development. In this case the illumination is no longer efficiently adjusted. This circumstance can be identified by the user when the background brightness of the field of view has an uneven distribution.

  • Page 28: Changing The Bulb

    6 Changing the bulb 6.1 Transmitted light OPM-1, OPO-1 You must not attempt to change the bulb immediately after the microscope has been used, as the bulb will still be hot and so there is a risk that the user could be burnt.

  • Page 29: Reflected Light

    Finally the lamp housing is screwed together again. 7 Changing the fuse 7.1 Transmitted light OPM-1, OPO-1 The fuse housing is on the rear of the microscope below the mains power supply socket. With the device switched off and unplugged, you can pull out the housing.

  • Page 30: Using Optional Accessories

    8 Using optional accessories 8.1 Camera connection (only for trinocular version) Due to the trinocular tube, which can be optionally fitted for the OPM-1, OPN-1 and OPO-1 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed.

  • Page 31: Trouble Shooting

    9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set...
  • Page 32 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...

  • Page 33: Service

    10 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN.

This manual is also suitable for:

Opn-1Opo-1

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