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Summary of Contents for BERTHOLD TECHNOLOGIES Junior LB 9509
- Page 1 LB 9509 Junior Operating Manual 32526BA2 Rev. Nr.: 07, 08/2018...
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Page 3: Table Of Contents
Contents Junior Contents Symbol Key ................... 4 Safety Instructions................. 5 Consignes de Sécurité ……………………………………………………..7 Overview ......................10 Instrument Set-Up ................... 14 2.1. Control Panel ..................15 Measuring Chamber ................16 Connections .................... 17 Battery Compartment ................18 Power Supply ..................19 Software .................... -
Page 4: Symbol Key
Symbol Key junior Symbol Key This symbol indicates a dangerous situation. Failing to heed this warning poses the threat of injuries or equipment damage! This character indicates important information. This character is used to indicate processing steps. This character marks enumeration. Messages on the junior's display are written in bold type and set in brackets [ ], for example [Measurement Start]. -
Page 5: Safety Instructions
DC- connector or pull out the wall power supply from main power. Use only the power supply provided with the instrument: (BERTHOLD TECHNOLOGIES ID-No.68052). FOX 18 power supply, Rating: Input: 100-240V AC; 50-60Hz; 400-200 mA; Class II Output: 5,9V DC / 3000mA... - Page 6 Always keep the sample holder clean! Avoid electrostatic charges! BERTHOLD TECHNOLOGIES accepts no liability (also in cases of third-party involvement) for damages caused through improper use of the instrument. For the safety of the user and to ensure good working condition, the proper steps for starting, testing and maintaining the instrument are to be followed.
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Page 7: Consignes De Sécurité
Consignes de Sécurité junior Consignes de Sécurité Instructions d'utilisation prescrites pour l'utilisation des applications Le junior est un luminomètre compact. Il est conçu pour les mesures biologiques et de chimioluminescence en utilisant des réactifs qui génèrent un rendement lumineux relativement constant. Les applications typiques sont par exemple: la recherche, les diagnostics clinique et la surveillance de l'hygiène. - Page 8 Utilisez uniquement le bloc d'alimentation fourni avec l'instrument (BERTHOLD TECHNOLOGIES. ID N ° 68052) avec une tension principale de 100V à 240V et une tension secondaire de 5,9 V DC / 3000mA. Le pôle négatif est à l'intérieur de la prise.
- Page 9 Assurez-vous que l'alimentation fournie est adaptée à votre tension d‘alimentation. Garder toujours le support d’echantillon proper. Éviter les décharges électrostatiques! BERTHOLD TECHNOLOGIES décline toute responsabilité de dommages résultant d’une utilisation non conforme à l’emploi prévu, y compris les dommages causés à des tiers.
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Page 10: Overview
1. Overview junior 1. Overview Intended Use Statement The junior is a compact luminometer which is suitable for measuring all types of ”glow” luminescence, i.e., bio- and chemiluminescent reactions that emit a constant light output over a given period of time. The junior may be used for the following purposes: ... - Page 11 1. Overview junior ... and operation is so simple The Display After the junior is switched on with the <ON> key, the Start display appears in the Display mode. The desired line can be selected using the arrow keys , and the <ENT> key activates the selected field (see figure 1.1): If you wish to select ...
- Page 12 1. Overview junior The Key Functions (also see Chapter 4.2) Switches on the instrument. Press “ON” again the display illumination can be switched on or off. Switches off the instrument. Activates the selected field: Menu field, Input or Selection field. Input and Selection mode: Confirms the entry or selection.
- Page 13 1. Overview junior How do I proceed? Step Chapter Page 1. Getting started 2. Define measurement protocol 3. Select measurement protocol 4. Measure Where in the manual is the explanation found? Function Chapter Page Start display Start measuring Site identification Test identification (in process) Selecting a measurement protocol Changing a measurement protocol...
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Page 14: Instrument Set-Up
2. Instrument Set-Up junior 2. Instrument Set-Up Function, quality and reliability were the values most important during development. On the front panel of the junior you have access to the measuring chamber containing the sample holder and photomultiplier (see Chapter 2.2) and the control panel (see Chapter 2.1). -
Page 15: Control Panel
Instrument Set-Up junior 2.1. Control Panel The control panel contains 6 function keys that operate the software and 2 keys that control the power switch, as well as the graphic display. display's background illumination is automatically switched to the energy-saving mode when the key pad has not been used for several seconds. -
Page 16: Measuring Chamber
2. Instrument Set-Up junior Measuring Chamber The measuring chamber contains both the sample holder and the photomultiplier. It can be opened using the black button. Only in its closed state the measuring chamber is light-tight, which is a prerequisite for luminescence detection. Individual test tubes are inserted into the sample holder for measurement. -
Page 17: Connections
Instrument Set-Up junior To open the sample holder: Press the black button. To close: The cover should be pressed down until it clicks into place. Only then is the chamber completely closed to light and measurement process enabled. The Photomultiplier (Detector) The photomultiplier (detector) is a single photon counter that operates in a wavelength range of 380 –... -
Page 18: Battery Compartment
(see chapter 2.5) Figure 2.5: Instrument underside with battery compartment Use only the recommended rechargeable battery! Ni/MH Akku Beltrona C4000 1.2 V/4000 mAh (BERTHOLD TECHNOLOGIES ordering number 64771) BERTHOLD TECHNOLOGIES accepts no liability for damages caused through use of other batteries! -
Page 19: Power Supply
When the instrument is connected to the power supply and it is switched on, the batteries will be charged. Simultaneously, the junior can be operated. Use only the power supply provided with the instrument (BERTHOLD TECHNOLOGIES ordering number 68052). Ni/MH 4000mAh Battery type Operating time without mains supply 4 –... -
Page 20: Getting Started
3. Getting started junior 3 Getting started Unpacking Unpack the instrument and power supply and check the instrument for damage. Immediately report any problems to the distributor or manufacturer. Connecting and Switching On Slide the protective panel on the rear of the instrument to the open position to access the connections (see figure 3.1). - Page 21 Getting started junior Switching on the instrument in battery mode: If the junior is not connected to the power supply, simply press the <ON> key. M e a s u re m e n t S ta rt A s s a y : A T P L o c a tio n : T e s t:...
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Page 22: Software Set-Up And Operation
4. Software Set-up and Operation junior 4 Software Set-up and Operation Set-up The junior's software is set up for easy measurement of standard applications. After switching on the instrument the measurement menu Measurement Start appears on the display. A measurement can be started immediately by pressing the <ENT>... -
Page 23: Operation
Software Set-up and Operation junior Operation The instrument is operated by use of 8 function keys. The readout follows on a graphic display. Results are given in Relative Light Units (RLUs), which means an integrated value over the measurement period. This RLU value is assembled by the measurement of RLU values in time intervals of 5 msec. -
Page 24: Selection Fields (Text)
4. Software Set-up and Operation junior key enables the value at the selected point to be raised or lowered using a scale of 0–9. Pressing the <ENT> key a second time confirms the value and exits the input mode. Example: Changing test number 111 to 121 Measurement Start Measurement Start Measurement Start... -
Page 25: Option Fields
Software Set-up and Operation junior 4.2.4 Option Fields A selected menu or a selected option can be identified by the extended cursor block covering the entire line. If a menu or option has been marked with the cursor, pressing the <ENT> key activates the selected menu. -
Page 26: The Keys
4. Software Set-up and Operation junior 4.2.5 The Keys Switches on the instrument. Press “ON” again the display illumination can be switched on or off. Switches off the instrument. In the Display mode: Activates the selected menu or switches to the Input or Selection mode. In the Input or Selection mode: Confirms the entry/selection and returns to the Display mode. -
Page 27: Setting The Parameter
5. Setting the Parameter junior Setting the Parameter In the [Settings] menu, the user- Measurement Start specific instrument Assay: Location: measurement parameters Test: defined. These parameters should be set following the first start-up of the instrument. The procedure for insert sample doing so is described in the next pages. -
Page 28: Password
5. Setting the Parameter junior Password If you want only authorized persons to change parameter settings and/or delete results, it is possible to define a password (max. 4 characters). Upon delivery, the junior's password has been defined as ”0000”. If this preset password is not changed, each password query can be bypassed by using the <ENT>... -
Page 29: User Input (Measurement Parameters)
5. Setting the Parameter junior User Input (Measurement Parameters) The measurement parameters are defined in the [User Input] sub-menu. A total of up to 6 different programs containing measurement parameters, respectively protocols can be saved for various measurement requirements. The desired program can then be selected with the [Assay] query in the Start display prior to measurement start. - Page 30 5. Setting the Parameter junior Press the <ENT> key in order to activate the Input mode and enter the RLU value for the [limit fail] which will serve as the upper/lower threshold value to classify results as fail. Confirm the entry with the <ENT> key. Move the cursor to the [O.K.] parameter and press the <ENT>...
- Page 31 5. Setting the Parameter junior In accordance with the defined threshold values, the results will be displayed and classified: If [limit pass] is smaller than [limit fail], RLU values, which are smaller than [limit pass], are classified as pass. If [limit pass] is higher than [limit fail], RLU values, which are higher than [limit pass], are classified as pass.
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Page 32: Date/Time
5. Setting the Parameter junior Date/Time The date and time for the internal real-time clock can be entered or changed. All results will be saved with corresponding time and date entries. These also serve as selection criteria for the readout on the display, the transfer of information to a PC, and the deletion of results. -
Page 33: Language
5. Setting the Parameter junior Language The junior's operation language is selectable. Procedure Settings Password In the [Settings] sub-menu, Customer Settings Date/Time select the [Language] option. Language RS232 Press the <ENT> key. System Select the desired language and press the <ENT> key in order to confirm the selection. -
Page 34: The Usb 2.0 Port
5. Setting the Parameter junior The USB 2.0 Port The USB 2.0 port on the rear of the junior enables the transfer of saved data (up to max. 2000 measurements) to a PC. This requires a USB Type B USB Type A cable. The transmission parameters necessary for your computer's configuration are to be set in the [RS232] menu. - Page 35 5. Setting the Parameter junior Selection: [1] or [2] [Flowcontrol] Selection: [XON/XOFF] = [Rts/Cts] = [None] = Pressing the <ESC> key, returns you to the [Settings] sub-menu. In order to transfer the selected data to your PC, first select the [Results] sub-menu in the Main menu and then the [PC] option.
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Page 36: The Lc-Display
5. Setting the Parameter junior The LC-Display Using this sub-menu, it is possible to modify brightness and contrast on the LC-display. Settings Password Procedure Customer Settings Date/Time Language In the [Settings] sub-menu, RS232 select the [System] option. System Press the <ENT> key. Select [Brightness] System:... -
Page 37: Measuring
6. Measuring junior Measuring To start a measurement in the Measure menu choose the [Measurement Start] option and press <ENT>. Parameters for the measurement can be selected and/or set in the Measure menu. This is achieved by moving the cursor to the appropriate line using the arrow keys activating the Input and/or the Selection mode with the <ENT>... -
Page 38: Measurement Procedure
Measuring junior Measurement Procedure Measurement Start Prepare the sample according Assay: to the reagent manufacturer’s Location: Test: instructions. Select desired insert sample measurement protocol and if necessary, enter a location, as well as a test identification number. Assay: Location: Open cover Test: measuring chamber and insert... - Page 39 6. Measuring junior Automatic Storing of Results The results are saved sequentially. If the memory is full (after the 2000th measurement), the oldest measurement data will be lost. Stopping a Measurement A measurement in progress can be stopped by pressing the <ESC>...
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Page 40: Processing The Results
7. Processing the Results junior 7 Processing the Results There are several options available in the [Results] menu for processing the stored results: The results, selected using Time and Site, may be transferred to a PC. The results, selected using Time and Site, may be shown on the LC-display. -
Page 41: Readouts On The Display
7. Processing the Results junior Readouts on the Display The results can be selected using the time of measurement and the site identification number and subsequently shown one after the other on the LC-display. Procedure Select the [Screen] option. Results Press the <ENT>... -
Page 42: Transferring Data To A Pc
7. Processing the Results junior Transferring Data to a PC The measured results can be selected using the time of measurement and the site identification number and subsequently transferred to a PC. Procedure: Connect the USB 2.0 port on the Results rear side of the instrument with Screen... - Page 43 7. Processing the Results junior Format of Data Transfer a) Data transfer to PC Data is transferred in the following order: Date Time Program Limit pass Limit fail Measuring time Location identification number Test identification number Result (= integrated RLUs) or ”Overload” <CR>...
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Page 44: Deleting Results
7. Processing the Results junior 7.3 Deleting Results The results to be deleted are Results selected using the time of measure- Screen ment as well as the site identification delete number shown before deletion on the display. Procedure Select the [Delete] option. Password Press the <ENT>... - Page 45 7. Processing the Results junior This causes the first measured result corresponding to the entered point in time and the defined site identification to be displayed. Page further with the key until you reach the readout of the result that you would like to display. Press the <ENT>...
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Page 46: Maintenance
8. Maintenance junior 8 Maintenance The junior is extremely maintenance-friendly and requires almost very little maintenance. Please observe the following: 1. Sample Holder The sample holder must always be kept clean and free from dust, in order not to spoil measuring results! ... -
Page 47: Trouble Shooting
9. Trouble Shooting junior 9 Trouble Shooting Error Cause Solution Display - 10 HV part could be Turn off instrument and let it dry. Switch on again. If error message appears again, contact service. other error contact service messages... -
Page 48: Technical Data
10. Technical Data junior 10 Technical Data Detector Ultra fast single photon counter with spectral sensitivity range of 380-630 nm Sensitivity 1 fmol ATP using the ATP Bioluminescence Assay Kit CLSII (Boehringer Mannheim, FRG) Dynamic range more than 6 decades Tube format 12 mm diameter, 47-75 mm height;...
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