BERTHOLD TECHNOLOGIES FlowStar2 LB 514 Operating Manual
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BERTHOLD TECHNOLOGIES FlowStar2 LB 514 Operating Manual

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Diese Geräte sind nicht für den Betrieb in explosionsgefährdeten Bereichen
bestimmt!
Die gelieferten Geräte dürfen nur vom Service der Firma Berthold oder
durch von der Firma Berthold autorisierte Techniker instand gesetzt wer-
den!
These units are not designed for use in hazardous areas.
The units supplied should not be repaired by anyone other than Berthold
service engineers or technicians authorized by Berthold.
Im Störungsfall wenden sie sich bitte an unseren zentralen Kundendienst.
In case of operation trouble, please address to our central service department.
BERTHOLD TECHNOLOGIES GmbH & Co. KG
Calmbacher Straße 22
D-75323 Bad Wildbad-Germany
www.berthold.com
Betriebsanleitung
Operating Manual
LB 514
FlowStar²
Id No.:62777 BA2
Rev. No.: 00
30.05.16
Phone +49-7081-177-0
Fax +49 7081-177-100
info@berthold.com

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Summary of Contents for BERTHOLD TECHNOLOGIES FlowStar2 LB 514

  • Page 1 Berthold. Im Störungsfall wenden sie sich bitte an unseren zentralen Kundendienst. In case of operation trouble, please address to our central service department. BERTHOLD TECHNOLOGIES GmbH & Co. KG Calmbacher Straße 22 Phone +49-7081-177-0...

  • Page 3: Table Of Contents

    FlowStar2 LB 514 Contents Introduction 1.1 Contents INTRODUCTION ..................... 3 Contents ......................3 Typographical Conventions................5 Safety Instructions ................... 7 Sicherheitshinweise ..................10 Consignes de Sécurité .................. 13 Carrying instructions ..................16 Overview ....................... 16 HPLC MONITOR ..................19 Measuring Cell Module ................. 20 Radioactivity Detector ...................
  • Page 4 Contents FlowStar2 LB 514 PARAMETER ENTRIES ................75 Configuration of the Device ................76 Nuclide Parameters ..................80 Defining / Editing Methods ................82 Measurement Parameters ................85 MEASUREMENT ..................89 10.1 Measurement Menu ..................89 10.2 Run Measurement ..................90 FUNCTION OF MEASURING CELLS ............

  • Page 5: Typographical Conventions

    FlowStar2 LB 514 Structure of User's Manual 1.2 Typographical Conventions We will use the following typographical conventions: Caution! Danger!  Important information! Example Menus and options in the FlowStar software are Measurement printed in bold type-face The selection of an option on a menu is written...

  • Page 7: Safety Instructions

    FlowStar2 LB 514 1. Introduction 1.3 Safety Instructions Special instructions and precautions Caution! This sign alerts you to important operating procedures with a potential danger of damaging the equipment and endangering your safety on disobeying. Refer to the user and instrument manuals for precautionary instructions.
  • Page 8 31 °C decreasing linearly to 50 % relative humidity up to 40 °C.  BERTHOLD TECHNOLOGIES assumes no liability for any damages, including those to third parties, caused by improper use or handling of the instrument. ...
  • Page 9 Any service and maintenance work not described in this user guide has to be performed by authorized service personnel. Use the instruments only for the designated application. Service address: Berthold Technologies GmbH & Co. KG Calmbacher Str. 22 D-75323 Bad Wildbad - Germany Tel. +49 (7081) 177-111...

  • Page 10: Sicherheitshinweise

    Anwendern empfohlen, diese Bedienungsanleitung vor Benutzung zu lesen.  Transportsicherungen vor dem Einschalten entfernen.  Die Geräte dürfen nur für den vorgesehenen Zweck eingesetzt werden.  Berthold Technologies übernimmt keinerlei Gewährleistung, auch für Schäden gegen- über Dritten, die durch unsachgemäße Handhabung der Geräte hervorgerufen wer- den.
  • Page 11 FlowStar2 LB 514 1. Introduction  Die Geräte dürfen nicht in Höhen von mehr als 2000 m über dem Meeresspiegel be- treiben werden.  Die Geräte sind dafür ausgelegt, innerhalb des Temperaturbereiches von 15 bis 40 °C betrieben zu werden.
  • Page 12 Überprüfungen und Wartungsmaßnahmen durchzuführen. Alle über die Betriebsanleitung hinausgehenden Wartungs- und Instandhaltungsmaßnahmen dür- fen nur von autorisierten Technikern ausgeführt werden. Serviceadresse: Berthold Technologies GmbH & Co. KG Calmbacher Str. 22 D-75323 Bad Wildbad Tel. +49 (7081) 177-111 Email: service@berthold.com...

  • Page 13: Consignes De Sécurité

    FlowStar2 LB 514 1. Introduction 1.5 Consignes de Sécurité Attention! Ce symbole d’alarme, vous avertit de prêter attention aux con- signes opératoires. En effet si vous ne suivez pas ces instructions, il peu y avoir un risque d’endommagement du matériel et également vous faire ecourir des risques pour votre propre sécurité.
  • Page 14 50% humidité relative pour une tempéra- ture d’ambiance allant jusqu’à 40 °C.  BERTHOLD TECHNOLOGIES décline toute responsabilité de dom- mages résultant d’une utilisation non conforme à l’emploi prévu, y com- pris les dommages causés à des tiers.
  • Page 15 Toutes les mesures d’entretien et de réparation allant au-delà de celles spéci- fiées dans ce manuel sont réservées aux techniciens autorisés. Berthold Technologies GmbH & Co. KG Calmbacher Str. 22 D-75323 Bad Wildbad Tel.

  • Page 16: Carrying Instructions

    1. Introduction FlowStar2 LB 514 1.6 Carrying instructions The FlowStar has 2 built-in photo multipliers and a heavy shielding. These are mounted in the front of the instrument. This means the center of gravity (cog) is located more to the front of the instrument.
  • Page 17 FlowStar2 LB 514 1. Introduction The liquid scintillator pump (LB 5037) is either controlled by the Radioac- ² tivity Monitor FlowStar (through serial port) or it is controlled manually. The monitor is operated via the touch screen monitor on the front panel of the device (see Figure 2).
  • Page 18 1. Introduction FlowStar2 LB 514 Further Berthold devices may be connected depending on the measur- ing configuration (see Figure 1):  scintillator pump LB 5037 required for the admixture method  T-mixer (also for the admixture method) and, if necessary, a mixing chamber (static mixer) ...

  • Page 19: Hplc Monitor

    FlowStar2 LB 514 2. HPLC Monitor HPLC Monitor Cell Cell LS-Pump LS-Pump Status Status READY READY Test 14C Test 14C Time Time HH:mm:ss HH:mm:ss 125.4 125.4 C-14 C-14 ELAPSED 00:00:00 ELAPSED 00:00:00 FlowStar LB 514 Figure 2: Measuring cell module and Radioactivity Monitor FlowStar²...

  • Page 20: Measuring Cell Module

    2. HPLC Monitor FlowStar2 LB 514 2.1 Measuring Cell Module The measuring cells are designed as modules and are therefore easy to replace. They are inserted into the instrument from the front and fixed by two screws. Each measuring cell includes a chip that offers two functions: ...

  • Page 21: Radioactivity Detector

    FlowStar2 LB 514 2. HPLC Monitor 2.2 Radioactivity Detector Nuclear radiation is detected using a scintillation counter, plus a coinci- dence circuit. The eluate passes through the measuring cell located between the cath- odes of two photomultipliers. The radiation in the eluate excites lumines- cent substances (e.g.

  • Page 22: Monitor Connections

    2. HPLC Monitor FlowStar2 LB 514 2.3 Monitor Connections All connection ports of FlowStar² are located on its rear panel (Figure 5), except for the measuring cell module (Figure 4) with the ports for the steel capillaries passing the eluate to [3] and from [4] the measuring cell. The measuring cell is inserted on the front panel and fixed by screws.
  • Page 23 FlowStar2 LB 514 2. HPLC Monitor USB connection A PC can be connected via the USB interface. The connection cable is included (USB A to USB B). Before connecting the FlowStar to a PC make sure the driver software was installed properly before. Otherwise the de- vice will not be discovered and installed in the Windows device manager.
  • Page 24 2. HPLC Monitor FlowStar2 LB 514 External Control Cable no.: Cable with loose ends for wiring as you choose For external control of FlowStar² via a HPLC control system or an au- tosampler. External Control Pin assignment Start: Start signal. A measurement is started by closing (edge) the Start and DGnd inputs.
  • Page 25 FlowStar2 LB 514 2. HPLC Monitor Analog Output Analog signals which are proportional to the radioactivity are output to the analog output pins for the integrator or an external evaluation system. The scale is set on the Methods menu (Methods parameters page 2). The in- formation in the dialog refers to 1 Volt: e.g.
  • Page 26 2. HPLC Monitor FlowStar2 LB 514 Mains Supply with Fuse Cable no.: ID. 80672 The device is connected to mains via the power socket (Figure 5 Mains) (DIN 49457 B, CEE 22) and the supplied power cord or another, suitable power cord.

  • Page 27: Leakage Sensor

    FlowStar2 LB 514 2. HPLC Monitor 2.4 Leakage Sensor FlowStar² includes a leakage sensor at the lowest point in the counting chamber and a hose to drain liquid. The leakage sensor responds to minor amounts of liquid which may es- cape from a defective measuring cell.

  • Page 28: Measuring Cells

    3. Measuring Cells FlowStar2 LB 514 Measuring Cells A number of different Berthold measuring cells are available for radioactiv- ity measurements in column eluates; all common sources can be meas- ured with high efficiency with these cells. Depending on the measuring method the cells are of different design (see Table 2).
  • Page 29 FlowStar2 LB 514 3. Measuring Cells Cell ID No. Remarks Max. pressure (bar) YG-50 S 5 P 50152 Preparative cell with surface-treated scintillator for high activities. Max. flow rate: 20 ml/min YG-150 U5D 50157 Cells with Yttrium/glass scintillator. YG-400 U5D...
  • Page 30 3. Measuring Cells FlowStar2 LB 514 a) Measuring cells with solid scintillator They consist of thin Halar or Teflon hoses filled with fine-grained scintillator grit (glass, Yttrium glass as scintillator). The scintillator is located stationary in the cell through which the eluate passes. Both ends of the cell incorpo- rate frits to prevent that the scintillator grit is flushed out of the cell.
  • Page 31 FlowStar2 LB 514 3. Measuring Cells b) Admixture method Liquid scintillator is continuously added to the column eluate, the resulting mixture passed through a cell and measured. Advantages of this method: virtually no memory effects and a high counting efficiency for all nuclides (e.g.
  • Page 32 3. Measuring Cells FlowStar2 LB 514 c) Measurement of Gamma Radiation Two types of cells are available for the measurement of gamma radiation: The J-1000 cell is used for low-energy Gamma radiation up to 50 KeV and is intended for the measurement of Iodine.

  • Page 33: Assembly And Disassembly Of Measuring Cells

    FlowStar2 LB 514 3. Measuring Cells 3.2 Assembly and Disassembly of Measuring Cells The measuring cells have been designed as a module for easy installation (and replacement) from the front of the monitor (see Figure 6). The module is fixed by 2 socket head cap screws size M 4 x 12 mm. (The screws and the respective screwdriver are included with the shipment.)

  • Page 34: Connecting Steel Capillaries

    3. Measuring Cells FlowStar2 LB 514 Excessive incidence of light into the measuring chamber has to be avoided, as otherwise the noise rate of the photomultiplier will increase and drop to the normal level only after several hours. If direct sun light and direct light...
  • Page 35 FlowStar2 LB 514 3. Measuring Cells min. ID 1 mm Cell Cell LS-Pump LS-Pump Status Status READY READY Test 14C Test 14C Time Time HH:mm:ss HH:mm:ss 125.4 125.4 C-14 C-14 ELAPSED 00:00:00 ELAPSED 00:00:00 ID 0,5 mm FlowStar LB 514 T-Mixer min.

  • Page 36: Cleaning The Measuring Cells

    3. Measuring Cells FlowStar2 LB 514 Installation procedure for fingertight screw fitting Figure 8: Capillary with PEEK "fingertight" screw fitting  Push capillary through the fingertight screw until a piece of it protrudes at the other end  Set screw onto the connecting piece and tighten it slightly.

  • Page 37: Cleaning The Measuring Chamber

    FlowStar2 LB 514 3. Measuring Cells 3.5 Cleaning the Measuring Chamber If the measuring cell has become clogged, for example by gel formation or bending of the outlet line, the cell may get damaged by the pressure of the HPLC pump, which may be up to 400 bar. As a consequence, eluate will flow into the measuring chamber.

  • Page 38: Scintillator Pump

    4. Scintillator Pump FlowStar2 LB 514 Scintillator Pump The HPLC pump LB 5037 supplies liquid scintillator for the admixture method. The pump is a high pressure double-head piston pump for con- stant liquid supply. More information how to operate the liquid scintillator pump can be found in the pump manual.

  • Page 39: Connecting The Supply System

    FlowStar2 LB 514 4. Scintillator Pump 4.2 Connecting the Supply System Supply of liquid scintillator: Outer diameter: 1/8", ID 2.4 mm The supply valve is located on the bottom right of the pump head. To en- sure a proper function, the supply line should have an internal diameter (ID) of at least 2 mm and not be longer than 1 m.
  • Page 40 4. Scintillator Pump FlowStar2 LB 514 The capillary path acts as resistance, so that a slight backpressure (ap- prox. 2 bar at 5 ml/min flow rate) will occur which is required for the exact switch function of the ball valves.
  • Page 41 FlowStar2 LB 514 4. Scintillator Pump  As soon as the liquid gets into the syringe without any bubbles, switch off the pump and place the liquid container on a lower level than the pump head while the syringe is still set. The underpressure created in the supply line automatically closes the inlet valve, so that the supply line (even with turned off pump) remains filled.

  • Page 42: Mixers

    4. Scintillator Pump FlowStar2 LB 514 4.3 Mixers There are 2 different types of mixers available. The t-mixer and a static mixer using 2 different cartridges (50 and 150µl). Both mixers are used to mix the scintillator flow coming from the pump with the eluate flow.

  • Page 43: Analytic Splitter

    FlowStar2 LB 514 5. Analytic Splitter Analytic Splitter 5.1 Description The use of the Analytic Splitter (Figure 10) requires a configuration (ad- mixture method) as illustrated in Figure 11. FC Cell 85% 15% Figure 10: Analytic splitter AS-15 E = Eluate input; FC = capillary to fraction collector; C = capillary to measuring cell The AS-xx splits off a certain fixed percentage (xx) for the radioactivity meas- urement.
  • Page 44 5. Analytic Splitter FlowStar2 LB 514 Column UV-Detector LS-Pump Cell 85% 15% Cell Cell LS-Pump LS-Pump Status Status READY READY Test 14C Test 14C Time Time 125.4 125.4 C-14 C-14 FlowS tar LB 514 Figure 11: Configuration with Analytic Splitter The AS-xx splits off a certain fixed percentage (xx) of the eluate flow toward the measuring cell (C).
  • Page 45 FlowStar2 LB 514 5. Analytic Splitter When setting up the configuration, please check if the connected detector (UV, IR, fluorescence, etc.) is capable of withstanding the backpressure generated. If this is not the case, connect the detector after the AS-xx, i.e.

  • Page 46: Installation

    5. Analytic Splitter FlowStar2 LB 514 5.2 Installation Do not increase or reduce the length of the capillaries which are firmly con- nected to the AS-xx at the outputs FC and C. Install the connections care- fully, since the internal diameter is only 0.1 mm.
  • Page 47 FlowStar2 LB 514 5. Analytic Splitter A solvent is pumped into the eluate input of the Analytic Splitter by a pump and the splitting ratio checked using 2 measuring cylinders. Caution! The flow rate must be absolutely free of pulsation. We recommend con-...

  • Page 48: Inspection And Trouble Shooting

    5. Analytic Splitter FlowStar2 LB 514 5.3 Inspection and Trouble Shooting If the AS-xx does not work with the splitting ratio indicated, check the AS- xx using the illustration above (Figure 13). Let the pump run for some time, then read off both measuring cylinders. If the liquid is not distributed in both cylinders in the splitting ratio indicated, this may be due to contamination of the capillaries or clogging of the splitter.

  • Page 49: Validation Using 3H- Und 14C-Test Sources

    FlowStar2 LB 514 6. Validation using 3H- und 14C-Test sources Validation using 3H- und 14C-Test sources H and C-Test sources are specially developed to check and validate the function of the FlowStar². The ID chip inside the test cell stores all rel- evant information which will be transmitted to the FlowStar²...

  • Page 50: Checking The Flowstar² With Test Sources

    6. Validation using 3H- und 14C-Test sources FlowStar2 LB 514 6.1 Checking the FlowStar² with test sources  Remove cell.  Install test source ( H or C) in place of the measuring cell. Please make sure that the cell and the test source will not see any light! This will cut down the decay period.
  • Page 51 FlowStar2 LB 514 6. Validation using 3H- und 14C-Test sources Validation 5.1.2010 TIME HH:mm:ss 4256.4 ELAPSED 00:00:00 PRERUN Current value Deviation 62546 Stat.error: nominal: 62856 Menu Parameters History Start Start Menu The instrument is in prerun mode. By pressing <Start> the validation...
  • Page 52 6. Validation using 3H- und 14C-Test sources FlowStar2 LB 514 History Pressing <History> from the validation menu will lead to the test cell se- lection. V A LIDA TIO N HIS TO R Y 1 / 1 Test H-3... Test C-14...
  • Page 53 FlowStar2 LB 514 6. Validation using 3H- und 14C-Test sources could give higher values due to the exposure to daylight. After the prerun time the measurement starts automatically.  Measurement Time Defines the measuring time for the validation (format hh:mm:ss).

  • Page 54: Startup Of The Hplc System

    7. Startup of the HPLC System FlowStar2 LB 514 Startup of the HPLC System 7.1 Preparations Check shipment Unpack the instruments and using the enclosed list of parts check if the shipment is complete; then check if the instruments show any sign of dam- age.
  • Page 55 FlowStar2 LB 514 7. Startup of the HPLC System p u rg e s ta rt/s to p p u rg e s ta rt/s to p HPLC-Controller Mixer Inj. Col. UV-Detector z e ro wa v e l e n g h t...

  • Page 56: Electrical Connections

    7. Startup of the HPLC System FlowStar2 LB 514 7.2 Electrical Connections The numbered cables (LB ...) are included in the shipment. Connect measuring system power phase. Connect the entire measuring configuration including all units (such as: gradient controller, UV or other detectors with connected evaluation units and computer) to one power phase;...
  • Page 57 FlowStar2 LB 514 7. Startup of the HPLC System Terminal Connections FlowStar 2 LB 514 Trä g e Ne tzsi ch e ru n g Sl o w Bl o w Fu se BERT HOLD T ECHNOLOGIES 7 5 3 2 3 Ba d Wi l d b a d Ma d e i n Ge rma n y Vor Öffnen des Gerätes...

  • Page 58: Installation Of The Measuring Cell

    7. Startup of the HPLC System FlowStar2 LB 514 7.3 Installation of the Measuring Cell Remove the counting chamber transport protection plate on the front panel of the HPLC Monitor which is secured by two Phillips screws (M4, 16 mm long).

  • Page 59: Installation Of The Capillaries At The Measuring Cell

    FlowStar2 LB 514 7. Startup of the HPLC System 7.4 Installation of the Capillaries at the Measuring Cell Use only steel capillaries for supply (IN) and disposal (OUT) of the eluate. Select suitable capillaries for your configuration (supplied or your own).
  • Page 60 7. Startup of the HPLC System FlowStar2 LB 514 Installation procedure for finger tight screw fitting (Figure 20) Figure 18: Capillary with PEEK "fingertight" screw fitting  Push capillary through the fingertight screw until a piece of it protrudes at the other end ...

  • Page 61: Installation Of Other Capillaries

    FlowStar2 LB 514 7. Startup of the HPLC System 7.5 Installation of Other Capillaries When using other monitors (e.g. UV monitors), the Radioactivity Monitor should always be the last link in the chain Only with solid cells: Connect the inlet capillaries as required by your measuring setup to the column or other monitors, respectively.

  • Page 62: Waste Valve Installation

    7. Startup of the HPLC System FlowStar2 LB 514 7.6 Waste valve installation Electrical connection The FlowStar² uses the (open collector) output labeled “Waste” to switch the waste valve from ‘cold’ to ‘hot’ Position. The two waste valve wires will be connected to the “Waste” pin and the “+12V”...
  • Page 63 FlowStar2 LB 514 7. Startup of the HPLC System Parameter definition After installing the waste valve the device has to be activated in the FlowStar² hardware configuration. When logged in as system administrator, select INSTRUMENT - > CONFIGURATION from the main menu.

  • Page 64: Software Installation

    7. Startup of the HPLC System FlowStar2 LB 514 7.7 Software Installation To control instrument features of the FlowStar² trough a PC a controller software is required. This software comes with the in- strument and allows complete remote control of the instrument as well as test functions.
  • Page 65 FlowStar2 LB 514 7. Startup of the HPLC System In these dialog box you can select what kind of extensions will be installed. The USB device driver is essential to communicate to the instru- ment and should not be disabled. Select the Chromeleon™ fea- ture only, when you intend to control the FlowStar²...

  • Page 67: Structure And Operation Of The Flowstar² Software

    FlowStar2 LB 514 8. Structure and Operation of the FlowStar² Software Structure and Operation of the FlowStar² Software HPLC measurements are controlled and evaluated by the FlowStar² soft- ware and presented on the display. The software is operated via the touchscreen monitor on the front panel of the FlowStar²...

  • Page 68: Operation

    8. Structure and Operation of the FlowStar² Software FlowStar2 LB 514 8.2 Operation The software is operated via the touchscreen monitor. A touchscreen monitor is screen and keyboard at the same time: To select a function or an item, push with a suitable pin (or your finger) on a function key, menu item or a parameter row.
  • Page 69 FlowStar2 LB 514 8. Structure and Operation of the FlowStar² Software Highlighting a row To highlight a row, either push this row or repeatedly push the function key Selecting parameters To select a parameter from a selection list you have to highlighting this list...
  • Page 70 8. Structure and Operation of the FlowStar² Software FlowStar2 LB 514 Info buttons (see also chapter 15. Troubleshooting Check List) <Status> button Shows the general system status. Push the <Status> button to go to the status page showing more information on the current system status, e.g.
  • Page 71 FlowStar2 LB 514 8. Structure and Operation of the FlowStar² Software <Cell> button Regular operation During regular operation, the current cell type is displayed as text next to the button. Push the button to view the cell parameters. In case of error If an error has occurred while reading the cell or if the counting chamber is open, then the text Error is displayed next to the button flashing inverse.

  • Page 72: Password Entry

    8. Structure and Operation of the FlowStar² Software FlowStar2 LB 514 8.3 Password Entry Before you can work with the system, you have to log-on with your user status so that the system knows which rights the respective user has.

  • Page 73: Menu And Function Overview

    FlowStar2 LB 514 8. Structure and Operation of the FlowStar² Software 8.4 Menu and Function Overview Measurement menu Main menu Status CelL LS-Pump READY Test 14C 4256.4 TIME M E A S U R E M E N T I N S T R U M E N T 432.4...
  • Page 74 8. Structure and Operation of the FlowStar² Software FlowStar2 LB 514 Configuration menus Main menu C E LL P A R A M E TE R S 1 / 1 Ident Number: 50153 C O N F I G U R A T I O N...

  • Page 75: Parameter Entries

    FlowStar2 LB 514 9. Parameter Entries Parameter Entries The parameter entries for the individual methods depend on the configu- ration of the device and the measuring cells used. Based on the configu- ration and the measuring cell used, parameters are automatically defaulted and only the relevant information is displayed, so that methods can be de- fined easily and safely.

  • Page 76: Configuration Of The Device

    9. Parameter Entries FlowStar2 LB 514 9.1 Configuration of the Device The device configuration is defined on the Configuration submenu.  On the measurement menu, select Menu | Device | Configuration. The first of three parameter pages is displayed. Configuration parameter page 1 / 3...
  • Page 77 FlowStar2 LB 514 9. Parameter Entries Jump: If the measurement curve has reached the right edge of the dis- play, the measurement image jumps to the left by half a display width. Scroll: The measurement image scrolls continuously to the left. If the graphic display starts to flicker, change to Jump.
  • Page 78 9. Parameter Entries FlowStar2 LB 514 Parameter page Configuration page 2/3 C O NFIG UR A TIO N 2 / 3 Liquid Handling LS-Pump installed: Fraction Collector installed: Waste Valve installed: Printer Printer installed: System Clock Time: 14:02:23 Date: 17.Aug. 2007 Miscellaneous Passwords...
  • Page 79 FlowStar2 LB 514 9. Parameter Entries System clock Time / Date Enter the current date and time. Parameter page Configuration page 3/3 Others Passwords Here you can enter one password for the user level and one for the de- vice administrator level.

  • Page 80: Nuclide Parameters

    9. Parameter Entries FlowStar2 LB 514 9.2 Nuclide Parameters The FlowStar² software includes a nuclide table on the Nuclide menu which contains the major nuclides for the HPLC methods including half-life period, energy thresholds and the measuring cells that can be used. The parameters of these nuclides cannot be changed by the user.
  • Page 81 FlowStar2 LB 514 9. Parameter Entries Permitted cells... Select the measuring cells (x) that are to be used to measure this nuclide. Only those nuclides are offered for selection in the method definition which are permitted for the installed/pre-selected measuring cell.

  • Page 82: Defining / Editing Methods

    9. Parameter Entries FlowStar2 LB 514 9.3 Defining / Editing Methods The parameters required for this configuration are defined and edited on the Methods menu depending on the device configuration and the installed measuring cell.  On the measurement menu, select Menu | Methods. The Methods window is displayed showing a list of 20 possible methods: Method 0 to Method 19.
  • Page 83 FlowStar2 LB 514 9. Parameter Entries Channel 1 Active Tick the checkbox (x) if you want to enable channel 1. Nuclide Select the desired nuclide. Push the button to view the selection list with the possible nuclides. Depending on the measuring cell used, only the nuclides are displayed that are permitted for this measuring cell (see nuclide table).
  • Page 84 9. Parameter Entries FlowStar2 LB 514 Measurement of one nuclide with two different analog output settings The measurement of one nuclide in two channels with different windows may be relevant when FlowStar² is integrated into an HPLC system through an alanlog output signal transfer. This will cause losses in presen- tation of the result, which are due to the small range of the analog signal (0-1 V) and the dynamic range (2 decades) of the HPLC system.

  • Page 85: Measurement Parameters

    FlowStar2 LB 514 9. Parameter Entries 9.4 Measurement Parameters The term measurement parameter refers to a group of parameters that are required for a run. They comprise the measuring cell parameters (de- faulted by the measuring cell that is used), a measurement method and the variables Run time and Start mode.
  • Page 86 9. Parameter Entries FlowStar2 LB 514 Measurement parameters page 1 Run name Enter the name of the run. This name is displayed on the Measurement menu while a measurement is running and it also appears on the printout. Run time Enter the duration of the run (max.
  • Page 87 FlowStar2 LB 514 9. Parameter Entries Measurement parameters page 2 M E A S UR E M E NT S E TUP 2 / 2 Fraction Collector Settings... Waste Valve Waste Valve Settings... Figure 30: Measurement parameters page 2 Fraction collector Is displayed only if the item Fraction collector is enabled Upon selection settings...
  • Page 88 9. Parameter Entries FlowStar2 LB 514 Time window Active from ... to ... Enter the time for enabling the fraction collector. Threshold value Only if you have selected Threshold in the controlled by field. Enter the low and high threshold value.

  • Page 89: Measurement

    FlowStar2 LB 514 10. Measurement 10. Measurement The device includes two measurement modes:  Continuous measurement: A continuous measurement starts automat- ically after the device has been powered up using the measurement parameters defined last (chapter 9.4). If you define parameters, for ex- ample, the measurement continues to run in the background.

  • Page 90: Run Measurement

    10. Measurement FlowStar2 LB 514 10.2 Run Measurement Instructions for starting/stopping a run  A run can be started any time on the Measurement menu by pushing S ta rt button. The measurement parameters defined last will be used (Measurement menu).
  • Page 91 FlowStar2 LB 514 10. Measurement Proceed as follows to define and start a run:  Select the Measurement menu on the Main menu. The Measurement parameters page is displayed.  Define the measurement parameters and select the desired measure- ment method as described in the previous section.

  • Page 92: Function Of Measuring Cells

    11. Function of Measuring Cells FlowStar2 LB 514 11. Function of Measuring Cells A number of different Berthold measuring cells are available for radioactiv- ity measurements in column eluates; all common sources can be meas- ured with high efficiency with these cells. Depending on the measurement...
  • Page 93 FlowStar2 LB 514 11. Function of Measuring Cells radioactivity events luminescence events PM 1 PM 2 coincidence Time 1 PM 2 PM 1 Time 2 PM 2 PM 1 T1: Time 1; T2: Time 2; PM1: Photomultiplier 1; Pm2: Photomultiplier 2 Figure 34: Illustrates the difference between luminescence and radioactive events Radioactive events occur in a “bundle”, so that both PM’s register photons (time 2),...
  • Page 94 11. Function of Measuring Cells FlowStar2 LB 514 The light signals are detected and amplified in the flow-through detector by 2 photomultipliers (PM) located on both sides of the measuring cell. The coincidence circuit separates and eliminates all single photon events (i.e.

  • Page 95: Flowstar² Controller Software

    FlowStar2 LB 514 12. FlowStar² Controller Software 12. FlowStar² Controller Software The FlowStar² can be connected to a PC using the USB inter- face. This feature of the FlowStar² allows a fully remote control and parameter setup by the included HPLC monitor or additional third party software if a dedicated software driver is implemented.

  • Page 96: Controller Backend

    12. FlowStar² Controller Software FlowStar2 LB 514 Messages Opens a message monitor to show the error log. Pause / Continue Remote Allows to pause the remote control which enables the user to op- erate the FlowStar² by using the touch screen. While remote con- trol is the touch screen locked.
  • Page 97 FlowStar2 LB 514 12. FlowStar² Controller Software Validation The “Validation” tab shows the dates of the validation process as well as upcoming test dates. In the subcategories Test H-3; Test C-14; Test BG; Test BGO the test results of the validation measurements as well as the first validation can be reviewed as a table or graph.
  • Page 98 12. FlowStar² Controller Software FlowStar2 LB 514 To print this report click on the printer icon in the icon bar.
  • Page 99 Advanced tools for software updates and monitoring are offered in this section. By clicking the controller connects with the Berthold Technologies webpage and checks if a newer version of the controller is available and downloads it after confirming. The tool "flash services" is only to install a new firmware version...
  • Page 100 This list can be saved as a .trs file by hitting the floppy disk icon. This file is useful for the Berthold Technologies Support to iden- tify communication problems and should be sent by email if this is requested by the suppport.
  • Page 101 FlowStar2 LB 514 12. FlowStar² Controller Software Presets In this section the Instrument presets like the setup of the analog acquisition channels, the scintillation pump type etc. are defined. The label of the signal, the voltage range and poliarity can be defined for each individual channel (see chapter 9.1).
  • Page 102 12. FlowStar² Controller Software FlowStar2 LB 514 Measure In this section the current measurement parameters can be re- viewed and changed if required. For further information about the measuring parameters refer to chapter 9.4 Test The Test features can be used during setup the instrument or to check if all accessories are properly connected and working.
  • Page 103 FlowStar2 LB 514 12. FlowStar² Controller Software In the Outport section the digital output (AUX) and analog outputs (Ratemeter) can be tested. Clicking on will set the AUX output, will reset the port. To check the analog ports just enter an output voltage (e.g. 1V) and click on to have this voltage present at the output.
  • Page 104 12. FlowStar² Controller Software FlowStar2 LB 514 is shown also. In the lower section are all measuring cells (checked boxes) shown who are allowed to use with this isotope. System In the “System” tab the brightness of the LC display and the timeout for the backlight will be set.
  • Page 105 FlowStar2 LB 514 12. FlowStar² Controller Software System System settings like the PMT control voltage and luminescence warning levels are visible in the “System” tab. PMT control volt- ages are read only. If "GLP Validation Strict Mode” is selected no further measure- ments will be possible if the validation is due.

  • Page 106: Hplc Data Monitor

    12. FlowStar² Controller Software FlowStar2 LB 514 12.3 HPLC Data Monitor The HPLC Monitor Software is a tool to check the instrument, run test measurements and qualify the results without using any other software. The Data Monitor can be launched through the Windows start Menu (Programs/Berthold/FlowStar²...

  • Page 107: Chromeleon Driver

    FlowStar2 LB 514 12. FlowStar² Controller Software For more information to the parameter setting refer to chapter 12.1 In the menu “Externals” the in- and outputs (waste valve, scintil- lator pump, analog output) can be tested. This function is used for service purposes to check the system setup (valves, A/D con- verter connection etc.).
  • Page 108 12. FlowStar² Controller Software FlowStar2 LB 514 Chromeleon 6.8 Chromeleon 7.x By selecting the context menu function “Add Device” (“Add Mod- ule” in CHROMELEON 7.x) a list of devices is indicated. Select as manufacturer “Berthold” to get a list of supported instruments.
  • Page 109 FlowStar2 LB 514 12. FlowStar² Controller Software to continue. The Driver will be initialized the first time. This could take a while depending on the controller status. When the initialization is finished the instrument configuration can be en- tered. In this dialog box the Instrument and signal names can be de- fined.
  • Page 110 12. FlowStar² Controller Software FlowStar2 LB 514 Here you can select the units (cpm, dpm, dps, Bq) for the cor- rected data channel. If is no correction wanted, use “cps” as unit or select the channel called “raw” channel for the acquisition. The "raw"...
  • Page 111 FlowStar2 LB 514 12. FlowStar² Controller Software Parameter entry is only possible if the waste valve is activated in the configuration of the FlowStar². After clicking on configuration is finished. By clicking on the dialog box will be closed and the FlowStar² is configured properly. The Server...
  • Page 112 12. FlowStar² Controller Software FlowStar2 LB 514 coming from detectors. This panel gives an overview on the system Status and shows all setted measurement parameters. By clicking on a differ- ent Setup can be selected and sent to the instrument.

  • Page 113: Program File Wizard (Only Chromeleon 6.X)

    FlowStar2 LB 514 12. FlowStar² Controller Software 12.4.2 Program File Wizard (only CHROMELEON 6.x) When running the program file wizard in CHOMELEON, the fol- lowing FlowStar² specific dialog boxes will show up All required signals must be checked. The channels labelled with “.raw”, represents raw data channels without any calculated cor-...
  • Page 114 12. FlowStar² Controller Software FlowStar2 LB 514 Start/Stop times of the signal acquisition and the operation of the scintillator pump can be selected in this dialog. When working with CHROMELEON 7.x the program file must be edited manually (see 12.4.3) The possible different configurations (measuring cells, flow rates etc.) are saved locally at the PC where the configura-...

  • Page 115: Sample Program File

    FlowStar2 LB 514 12. FlowStar² Controller Software 12.4.3 Sample Program File A sample program file is shown below. The FlowStar² relevant commands are highlighted. fs_ActiveConfiguration = "Default" UV_VIS_1.RefBandwidth = 100 [nm] UV_VIS_1.Step = Auto UV_VIS_1.Average = Flow = 1.000 [ml/min] %B = 20.0 [%]...

  • Page 116: Explanations

    13. Explanations FlowStar2 LB 514 13. Explanations 13.1 Peak Half-Width - Time Constant - Smoothing An absolutely essential requirement for a good on-line peak de- tection in the HPLC integrator is the best possible fit of the ratemeter signal (analog output signal) to the expected peak shape - especially the peak half-width.
  • Page 117 FlowStar2 LB 514 13. Explanations group is defined by the length of the basis time interval. A maxi- mum of 8 groups will be formed (see table 2, column 2), always starting with the counts measured last. Since the basis time in- terval is max.
  • Page 118 13. Explanations FlowStar2 LB 514 To ensure that a peak will not flatten out too much when averag- ing over a wide range, the basis time intervals are weighted less strongly at the beginning and end than those in the middle of the interval (see Table 2, column 3).

  • Page 119: Efficiency Correction

    FlowStar2 LB 514 13. Explanations 13.2 Efficiency Correction The efficiency correction is affected by the parameters 5. through By taking into account the empirically determined static and dy- namic efficiency, the cpm or cps values calculated as a function of the ratemeter unit can be converted such that the activity flow is output in dpm/min or dps/min;...

  • Page 120: Disturbances

    14. Disturbances FlowStar2 LB 514 14. Disturbances This chapter summarizes all possible disturbances, describes them, ex- plains their cause and provides information on how to eliminate them. 14.1 Electrical or Mains Disturbance Sparks generated by old refrigerators, centrifuges, electrical switches may enter the measuring instrument through the mains supply or cables and cause disturbances in the detector or in the digital unit.
  • Page 121 FlowStar2 LB 514 14. Disturbances Type Scintillator used Quantity (Cell volume) Existing ambient radiation BG of cell Discriminator setting Cell contamination BG total (mainly in solid cells) Device BG Cross-talk Random coincidence Noise Figure 35: Factors affecting the background If the BG values are significantly higher than the values listed in the table, check first whether this is caused by the measuring cell or the instrument: ...
  • Page 122 14. Disturbances FlowStar2 LB 514 Possible reasons and solutions:  The newly installed solid cell has not yet decayed (mainly with YG, J-1000, BGO-x cells). Wait until the cell has decayed.  The solid cell is contaminated. Replace cell or decontaminate it.

  • Page 123: Luminescence Effects

    FlowStar2 LB 514 14. Disturbances 14.3 Luminescence Effects This chapter summarizes all those effects which cause a signal in the ra- dioactivity channels even though no radioactivity is present in the measur- ing cell. The spectral distribution (channel ratio) indicates the cause. De- pending on the spectral distribution, the following phenomena may occur: ...
  • Page 124 14. Disturbances FlowStar2 LB 514 Luminescence caused by incomplete mixing The mixing process in the measuring cell is not yet completed. One reason is, for example, that the scintillator mixes only badly with the solvent. In addition to heat, light and luminescence are also generated. Open the measuring cell inlet, put the stainless steel capillary in a transparent glass container and let the cocktail flow off inside the glass.

  • Page 125: Troubleshooting Check List

    FlowStar2 LB 514 15. Troubleshooting Check List 15. Troubleshooting Check List General Information Error description Possibly cause/Remedy No function, Check mains voltage, mains connection, power on switch, Display or mains cable, fuses. LED dark Measurement OK. Compare PM HV control voltage value with datasheet (delivered with the instru- Display "0 cpm"...

  • Page 126: Instrument Cleaning

    15. Troubleshooting Check List FlowStar2 LB 514 Display "Warning" when the Status button is pushed: The measurement is not stopped if "Error" is displayed. Push the Status button to view further information on the error: Error info after pushing the...

  • Page 127: Technical Data

    FlowStar2 LB 514 16. Technical Data 16. Technical Data Detection Unit State of the Art ultra sensitive 2 inch PMT detection system with random coincidence counting and luminescence subtraction. Display Monochrome graphical touch screen with 320 x 240 pixel resolution Communication USB 2.0 (B-Type connector), RS-232 port for scintillator pump control...

  • Page 128: Index

    17. Index FlowStar2 LB 514 17. Index Consignes de Sécurité Control voltage for detectors Acetonitril buffer gradient Admixture cells Admixture method Defining / Editing methods Alphanumeric entries Defining and starting a run Analog Input Delay time Analog input settings Display configuration...
  • Page 129 FlowStar2 LB 514 17. Index Identification of the cells Operation of the software Info buttons 68, 70 Outlet capillary 30, 34 Overview Menus and functions J-1000 cell Parameter entries Parameter page configuration Leakage sensor 27, 37 Password 72, 79 Light seal...
  • Page 130 17. Index FlowStar2 LB 514 Test sources Time constant Validation Touchscreen monitor Vial volume Typographical conventions Warning Waste Waste valve parameters...

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