1. Manuals
  2. Brands
  3. Photon Systems Instruments Manuals
  4. Measuring Instruments
  5. FMT150
  6. Instruction manual

Photon Systems Instruments Photobioreactor FMT150 Instruction Manual

Hide thumbs Also See for Photobioreactor FMT150:
Table of Contents

Advertisement

Quick Links

Download this manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Photobioreactor FMT150 and is the answer not in the manual?

Questions and answers

Related Manuals for Photon Systems Instruments Photobioreactor FMT150

Summary of Contents for Photon Systems Instruments Photobioreactor FMT150

  • Page 2: Table Of Contents

    General Description ..............................5 Device Description ..............................6 List of different Photobioreactor FMT150 types ....................8 List of parameters measured and/or calculated by the Photobioreactor FMT150 ......... 10 Components of Photobioreactor FMT150....................... 11 Description of the Photobioreactor Control Unit Front Panel ................ 20 Description of the PBR Rear Panel for version FMT150.1 ................
  • Page 3 Appendix 4 – O Electrode Calibration ........................75 Appendix 5 – CO Electrode Calibration ........................ 76 Appendix 6 – Conversion table for CO calibration ....................82 Appendix 7 – GAS 150 Technical specifications ....................83 Appendix 8 – QY measurement ..........................84 Appendix 9 –...

  • Page 4: Instruction Manual

    INSTRUCTION MANUAL WARNINGS AND SAFETY PRECAUTIONS PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE TURNING THE PHOTOBIOREACTOR ON:  Remove all packaging and transport protectors before connecting the photobioreactor to the power supply.  Use only cables supplied by the manufacturer. ...

  • Page 5: General Description

    In addition, up to 8 peristaltic pumps for different chemostat or pH-stat cultivation control can be linked to the Photobioreactor FMT150 for the highly precise control of cultivation conditions.

  • Page 6: Device Description

    DEVICE DESCRIPTION Standard version of the Photobioreactor FMT150 consists of a cultivation vessel with a sealable lid and a base box containing electronics circuitry, LED light panel (Fig. 1A) and other components essential for the optimal operation of the photobioreactor.
  • Page 7 densitometer. F) Schematic representation of optional gas supply components and modules that can be used to stabilize aeration and set CO concentration level. The array of high-power light emitting diodes (LEDs) is located behind the culture vessel. These LEDs generate a highly uniform irradiance flux that can be controlled in the range 0-3000 mol (photons)/m PAR.

  • Page 8: List Of Different Photobioreactor Fmt150 Types

    LIST OF DIFFERENT PHOTOBIOREACTOR FMT150 TYPES The Photobioreactor is currently manufactured in three types enabling the customer to select the optimal system with respect to volume. Various options for light quality, light intensity and thermoregulation are also available. Numerous additional accessories such as various sensors can be supplied as described below on page Please note that this manual describes operation and use of two available PBR FMT150 versions (Fig.
  • Page 9 TEMPERATURE CONTROL: Requires presence of the pH/Temperature probe. - Standard range of 18 – 50 °C (static or ramping). In table below please find approximate estimation of time required for the given temperature equilibration. Initial Target Light conditions Equilibration time temperature temperature µmol/m...

  • Page 10: List Of Parameters Measured And/Or Calculated By The Photobioreactor Fmt150

    LIST OF PARAMETERS MEASURED AND/OR CALCULATED BY THE PHOTOBIOREACTOR FMT150 Symbol Name Description (Dark) Instantaneous fluorescence in Instantaneous F-yield in dark equals F the dark (in dark-adapted sample) (Light) Instantaneous fluorescence in Instantaneous F-yield in light that results the light...

  • Page 11: Components Of Photobioreactor Fmt150

    (as listed below) plus air pump with bubble interruption valve and pH/temperature module. Components that are part of minimal Photobioreactor FMT150 configuration are shown in Fig. 5. Optional components that are available as accesory modules and can be purchased together with Photobioreactor FMT150 are described in next section on page 14.
  • Page 12 Version FMT 150.1 Version FMT 150.2 Fig. 4 Components of the spare part kit. Numbers correspond to component number in table 1. Table 1: List of spare part kit components shown in Fig. 4. Component Component Description Specification Explanation Number Male Luer Integral Lock MTLL240-J1A* 5/32”...
  • Page 13 Fig. 5 Minimal configuration set of the Photobioreactor FMT150. Numbers correspond to numbers of components as described on page 11 and 14.
  • Page 14 Bubble interruption valve can be purchased also without air pump if required. pH/Temperature module - To measure pH and temperature. It is required for temperature control of photobioreactor. The module consists of electronic unit built-in Photobioreactor FMT150 Control Unit, sensor probe and probe cable (Fig. 6). InPro3253SG/120/PT1000 or InPro3253/120/PT1000 single rod measuring cells from METTLER TOLEDO (www.mt.com) are supplied as low maintenance,...
  • Page 15 Module Module - To determine the dissolved carbon dioxide concentration in the medium. The module consists of external electronic unit for connection with Photobioreactor FMT150 Control Unit, sensor probe and probe cable (Fig. 8). InPro5000/12/120 sensor from METTLER TOLEDO (www.mt.com) with integrated temperature probe is used for potentiometric CO concentration measurement.
  • Page 16 consistent stirring around the probes is essential to accurate measurements of dO and dCO . It can be also used to help reduce cell sedimentation or clumping when used together with gas bubbling. Note: Magnetic stirrer cannot be used simultaneously with an external light panel as they both fit on the outside of the vessel.
  • Page 17 photobioreactor the use of one PWM pump per photobioreactor is required to supply portion of the gas from the gas mixing system to the bioreactor without affection the gas flow between the bioreactors. Fig. 11 GMS 150. Gas Analysis System GAS 150 - Enables measurements of CO gas in the head space of the cultivation vessel as it leaves via the vessel gas output (Fig.
  • Page 18 Fig. 13 Peristaltic Pump PP500. Remote Control System for PBR FMT150 - Remote Control System for PBR (Fig. 14) includes a PC with photobioreactor software for data collection, data visualization and remote control of photobioreactor. The software operates in Linux operating system and allows set up of user-defined protocols;...
  • Page 19 Supplementary O Probe–Additional sensor probe to O module. Membrane Kit - O membrane replacement kit for O probe. Includes: 4 membrane bodies, 1 O- ring set, 25 ml electrolyte. Membrane Kit - CO membrane replacement kit for CO probe. Includes: 4 membrane bodies, 1 O-ring set, 25 ml electrolyte.

  • Page 20: Description Of The Photobioreactor Control Unit Front Panel

    DESCRIPTION OF THE PHOTOBIOREACTOR CONTROL UNIT FRONT PANEL Photobioreactor Control Unit front panel can be used for simple manual control of the PBR FMT150. By using the keys on the front panel the operation of cultivation and monitoring units such as pH/temperature sensor, light control, OD monitoring module, PWM modeor CO and O sensor can be controlled.

  • Page 21: Description Of The Pbr Rear Panel For Version Fmt150.1

    The descriptions of the menus that are controlled by the MAIN KEYS are in Appendix 2 on page 66. NOTE: Please refer to the MENU Tree description guideline if Photobioreactor FMT150 is purchased without the Remote Control System for PBR FMT150 and the PBR FMT150 is to be controlled solely manually via the control unit front panel.
  • Page 22 PRIOR CONSULTATION WITH THE MANUFACTURER. 8. IDENTIFICATION LABEL WITH SERIAL NUMBER Each Photobioreactor FMT150 produced has a serial number that corresponds with the serial number of the power supply unit. These are always provided together and are not interchangeable. Prior to switching PBR FMT150 device ON make sure that the serial number on the rear panel of the main body matches the serial number on the power supply (Fig.

  • Page 23: Description Of The Pbr Rear Panel For Version Fmt150.2

    Warning: Damage can occur when the power supply is incompatible with the PBR FMT150 device. DESCRIPTION OF THE PBR REAR PANEL FOR VERSION FMT150.2 The rear panel houses connectors for all connecting cables (Fig. 17). Please note that the numbers in the next section correspond to the numbers of connectors in Fig.
  • Page 24 The power supply unit is plugged into the FMT150 control unit via this connector. 10. IDENTIFICATION LABEL WITH SERIAL NUMBER Each Photobioreactor FMT150 produced has a serial number that corresponds with the serial number of the power supply unit. These are always provided together and are not interchangeable. Prior to switching PBR FMT150 device ON make sure that the serial number on the front panel of the main body matches the serial number on the power supply (Fig.
  • Page 25 Table 2: DEVICE USED PROPER DEVICE CONNECTOR INTERCONNECTION POSSIBLE Peristaltic Pump AUX 1/2/3/4 module AUX 1/2/3/4 External Light AUX 1/2/3/4 Bubble Interruption Valve AUX 2 Magnetic Stirrer AUX 1 PWM Pump AUX 1 Important: Please note that Bubble Interruption Valve must be always connected to the AUX2 connector and Magnetic stirrer and PWM Pump always to connector AUX1.

  • Page 26: Installation

    INSTALLATION DEVICE INSTALLATION To install the device properly, it is necessary to follow the proper sequence of the assembly instructions. Instructions for assembly of standard PBR FMT150 (400, 1000 and 3000 ml) are described below: CONNECTING THE POWER SUPPLY TO THE PHOTOBIOREACTOR ...
  • Page 27 OPERATION  Plug the AC power cord into an electrical outlet (Fig. 19B,C)  Switch ON the power supply  Switch on the Mains ON/OFF key on the front of the PBR Control Unit  IMPORTANT: It is necessary to allow 20 minutes stabilisation time for the device prior any experiment or calibration is performed.
  • Page 28 The lid of the vessel is fastened to the vessel via two screws (n°3 in Fig. 21C) using the hex key provided. The lidhas two holders built in for easy handling of the vessel in and out of the photobioreactor (n°2 Fig. 21C). There are four openings for luer connectors.
  • Page 29 Inlets for the gas dispenser tubing. (5) Connectors for optional sensors closed with filler screws. (6) Sampling tube. (7) Excess medium outlet. (8) pH/temperature sensor. CULTIVATION VESSEL MAINTENANCE The whole vessel is fully autoclavable, including the detachable lid, connectors, sensors, and silicone tubing. However, keep in mind that most materials deteriorate after repeated autoclaving.
  • Page 30 (described on page 11) and is recommended for purchase if precise control of cultivation conditions is required. The module consists of electronic unit built-in Photobioreactor FMT150 Control Unit, sensor probe and sensor communication cable (Fig. 6).
  • Page 31 FMT150 is used values measured by pH/temperature sensor are automatically displayed in graphical interface shown in Fig. 49 on page 57. Fig. 23 Installation of the pH/temp sensor. A) Positioning the pH/temp probe. B) Securing the pH/temp probe. C) Attaching the cable to the positioned probe.
  • Page 32 Fig. 24 pH sensor calibration. A) Electrode is placed into the solution of pH 7. B) Reading of the measured value. C, D, E) Grounding with the copper cable for the calibration of InPro3253/120/PT1000 version of the electrode. GAS SUPPLY INSTALLATION The gas supply to the photobioreactor consists of four main parts –...
  • Page 33 end of the silicon tubing from the humidifier bottle and via other BSFTLL Luer Lock connect with the silicon tubing coming from the bubble interruption valve. Fig. 25 Assembly of the bubble humidifier bottle. A) Components needed for the assembly of the bottle. B) Assembled lid. C) Assembled bubble humidifier bottle.

  • Page 34: Accessories/Optional Components

    O concentration in the culture medium. The module consists of electronic unit built-in Photobioreactor FMT150 Control Unit, sensor probe and sensor communication cable (Fig. 7). Please note that the calibration of the O...

  • Page 35: Co 2 Electrode Installation

    The module consists of external electronic unit for connection with Photobioreactor FMT150 Control Unit, sensor probe and sensor communication cable (Fig. 8). Please note that CO...
  • Page 36 Fig. 29 Installation of the CO sensor. A) Positioning the electrode. B) Attaching of the electronic module to the electrode. C) Attaching of the communication cable to the “AUX IN” connector of electronic module. D) Attaching of the communication cable to the “AUX 1” connector at the rear of the PBR. ...

  • Page 37: Magnetic Stirrer Installation

    MAGNETIC STIRRER INS TALLATION The magnetic stirrer module is designed for homogenous mixing of the culture inside the cultivation vessel. The magnetic stirrer rotates a teflon-coated magnetic bar in a vertical plane on the inside of the front glass panel of the cultivation vessel.
  • Page 38 Version FMT 150.1 Version FMT 150.2 Fig. 31 Installation of the magnetic stirrer. A) Screws for fixing the stirrer to the front side of the PBR main body. B) Magnetic stirrer is fastened in the correct position to the front part of the PBR. C) Attaching of the communication cable to the “AUX 1”...

  • Page 39: External Light Panel Installation

    EXTERNAL LIGHT PANEL INSTALLATION The Photobioreactor can be supplemented with an additional Light Source that helps to accommodate specific needs of different organisms or to increase illumination intensity. Please note that the external light source cannot be used simultaneously with the magnetic stirrer. One or the other can be used at one time. Please note that the following instructions are for PBR FMT150.1 version additional light panel installation.

  • Page 40: High-Precission Gas Mixing System

    (1000 ml/min).The GMS 150 can be used as a stand-alone instrument with other applications or as an additional and optional component of the Photobioreactor FMT150. Please note that the GMS 150 works independently of the PBR software.
  • Page 41 Note: GM system is a separate instrument and is operated independent of the PBR FMT150. GM System settings and operation is controlled solely via the front display panel as described in the GM System Instruction manual.  When more than one PBR FMT150 units are connected to the same GM system the set up of such connection scheme is shown in Fig.
  • Page 42 Fig. 35 GM system connection with the PBR FMT150. A) Mixed gas of given composition (1) is pumped from GM System via (2) to the PBR FMT150 via air interruption valve (3). B) Vessel lid assembly. (4) Air distribution tube, (7) Medium outlet, (6) Sampling tube.

  • Page 43: Peristaltic Pump Installation

    PERISTALTIC PUMP INSTALLATION Peristaltic pump allows for different continuous chemostat or pH-stat cultivation control. These pumps can be operated in pH-stat or chemostat mode and each pump can be controlled according to a different parameter. In turbidostat mode when Turbidostat Module I. software controller is purchased continuous-flow turbidostatic cultivation is enabled.
  • Page 44 Fig. 37 Final assembly of the peristaltic pump. A) Second luer connector is attached. B) Connection of the silicone tubing with the culture for the cultivation. C) Fully installed peristaltic pump tubing. D) Schematic representation of PBR FMT150 unit connected to peristaltic pumps in chemostat and turbidostat mode and to GMS 150. ...
  • Page 45 Fig. 38 Connection of peristaltic pump with PBR FMT150.2. A) Rear part of the Peristaltic Pump showing “AUX IN” connector. B) AUX1 connector of the PBR for the peristaltic pump connection. C) Power cord connection of peristaltic pump. D) Final setup of the peristaltic pump with the PBR. ...

  • Page 46: Gas Analysis System Gas 150

    The GAS 150 can be used as a stand-alone gas analysis system or it can be incorporated into a system with Photobioreactor FMT150. Please refer to Appendix 7 on page 83 for detail technical specifications of GAS 150.
  • Page 47  Connect the GAS 150 to the gas source (n°2 in Fig. 41). Here the gas source comes from the Gas Mixing System (GMS 150)(n° 1).  Connect the OUTPUT GMS tubing (n°1 in Fig. 41) with GAS input (n°2 in Fig. 41) via a T-parker connector with a check valve (n°7 in Table 1) (Fig.
  • Page 48 3-GAS output for bringing the mixed air to humidifier bottle (4) and to the PBR FMT150. 5-Input for gas from the cultivation vessel to GAS 150. 6-Air from the vessel passes through dehumidifier prior CO concentration analysis. Important note: If peristaltic pump in chemostat or turbidostat mode is connected with the PBR FMT150 together with GAS System the vessel lid configuration with the tubing assembly is different as compared to setup with no chemostat/turbidostat module and no GAS.
  • Page 49 Fig. 43 Vessel lid with standard and GAS aeration U-tubing.Pictures D-I show individual steps for changing the standard aeration tubing and exchange with GAS aeration U-tubing Note: Use spanner (n°14 in table 1) to release the value plastic connectors from the vessel lid.

  • Page 50: Pwm Pump

    (2) Single gas input into the vessel; (3) When connected to the vessel inlet and outlet (closed flow mode), it can be used for suspension mixing (bubbling) in anaerobic conditions. Instructions below explain connection of the PWM Pump with the Photobioreactor FMT150: ...

  • Page 51: Photobioreactor Software

    PHOTOBIOREACTOR SOFTWARE To control the operation of the Photobioreactor FMT150 with the photobioreactor software connects Photobioreactor via the serial cable and the USB adaptor to the computer (Fig. 45). Fig. 45 USB adaptor to Serial cable is plugged to the connector in the rear panel of PBR FMT150.2.
  • Page 52 If more than one Photobioreactor is connected to the computer each will have a separate sub-tab under the Devices tab. These sub-tabs appear/disappear as different PBR devices are connected or disconnected from the computer (Fig. 46, arrow 1). Selection of the connected devices as well as start and set up of the experiments is done from this tab. The connected Photobioreactors can be idle or running an experiment.
  • Page 53 Fig. 46 The Devices tab software window in an idle mode. SETTING UP AN EXPERIMENT  To set the conditions and parameters of a new experiment press the “Start experiment” button (Panel 4, Idle Controls, Fig. 46). Devices Tab will switch from idle mode to Protocol window (Fig. 47). ...
  • Page 54 Fig. 47 The Protocol window in which experimental protocol is defined.  The graphical representation scheme shows the 24h cycle light modulation for both actinic light and thermoregulation. The first phase of the red light cycle is in a sine modulation with a phase offset 0, meaning here that the red irradiance starts from a sine minimum towards a maximum.
  • Page 55 in the first phase of the cycle goes from 0 µE at the beginning (time 0) to 30 µE of irradiance at the end of the phase duration (3h 30 min). During the second phase of the cycle, the red light remains constant at 30 µE for the following 8 hours (from 3h 30 min to 11h 30 min).
  • Page 56  QY stands for all measured parameters listed above including those requiring saturating light flash such as Quantum Yield of PSII (QY). The “non-disturbing” measurements are executed in parallel. For more information about QY measurements please refer to Appendix 8. Before starting an experiment, it is recommended to record the Experiment info (Fig.
  • Page 57 Fig. 49 Sample data from last two days of an experiment in progress. BROWSING OF RECORDED EXPERIMENTS AND DATA EXPORT In the Recorded experiments tab (Fig. 50) you may view, delete and export completed experiments. Experiments from all photobioreactors connected to the given PC will be displayed. ...
  • Page 58 Fig. 50 Recorded experiments. SETTINGS Under the Settings tab, you may set the time of the system. IMPORTANT NOTE: most of the internal components rely on timing. Resetting the time will reset the components used for controlling the devices so that any running experiment would be aborted by time resetting.
  • Page 59 Fig. 51 Settings tab DEVICE PAIRING Under the Settings tab, you may view optional accessories such as Gas Analysing module that are connected and operated by the Photobioreactor software. Each device connected to the Photobioreactor module has its own ID number. Make sure that correct ID of the devices that are used is linked in the Device Pairing tab with the given Photobioreactor.
  • Page 60 REMOTE ACCESS The graphical user interface is a separate application which communicates with the system over network (even when started locally).  To access the system remotely, e.g. from your office, home or hotel, enter the address of the system in a web browser of your computer and click on the "bio reactor gui"...
  • Page 61  press Ctrl+d to close the terminal.  Use the IP address assigned (as marked above in red) and search for this address in your web browser to access the system remotely. Fig. 54 Remote access of the Photobioreactor system.

  • Page 62: User´s Guide For Cultivation Of Algae And Cyanobacteria

    USER´S GUIDE FOR CULTIVATION OF ALGAE AND CYANOBACTERIA This section describes show to set up a standard Photobioreactor FMT150 and how to initiate cultivation of an algal culture. PREPARATION AND SET UP OF THE COMPONENTS First prepare the glass cultivation vessel and its lid. Follow the steps described on page 29 to assemble the vessel.
  • Page 63 Connect all tubing for gas inlet and gas/medium outlet and sampling as described in Fig. 22. Close all openings, except the one for medium outlet, using sterile connectors. Take the assembled vessel out of the hood and place itin the PBR,lock it with the side clips. Connect the pH/temperature probe with the PBR (as described on page 30).

  • Page 64: Appendix 1 - Installation Of The Operating System

    APPENDIX 1 – INSTALLATION OF THE OPERATING SYSTEM When Photobioreactor system is purchased with the operation software and the computer, the software will be pre-installed and the following procedure does not need to be executed. The installation of the operating system of the control computer is only necessary when new version of the operation system should be installed or when the customer is asked by the manufacturer to perform the software re-installation.
  • Page 65 If the network installation is selected, you will be asked to choose a network interface which will be used for the connection. If your system has more than one network card, you may identify the correct one by the status indicated next to the card name (e.g., “dhcp capable”...

  • Page 66: Appendix 2 - Control Menu Tree

    Finally, the installer checks the connection to the remote PSI repository. APPENDIX 2 – CONTROL MENU TREE This section explains graphically the operation of the Photobioreactor FMT150 control unit without the Remote Control Software for PBR FMT150. Photobioreactor can operate in cultivation mode without the Remote Control Softwarefor PBR FMT150 by setting the cultivations parameters such as light, temperature, etc.

  • Page 67: Explanation Of Control Keys And Used Symbols

    1. Main Menu 2. Four Sub-Menus (Measure, Lights, Thermoregulation, Setting) 3. First-level nested structures 4. Second-level nested structures The following instructions describe operation of the PBR FMT150 in cultivation mode only. EXPLANATION OF CONTROL KEYS AND USED SYMBOLS The Photobioreactor is operated by five keys: ...

  • Page 68: Graphical Presentation Of The Operation Schematic

    GRAPHICAL PRESENTATION OF THE OPERATION SCHEMATIC...

  • Page 74: Appendix 3 - Detailed Explanation Of The Pulse Light Control

    APPENDIX 3 – DETAILED EXPLANATION OF THE PULSE LIGHT CONTROL Pulse light control mode is designed to study the response of the cultivated culture to rapidly repeated flashes of actinic lights (red, blue or white combination depending on purchased combination of actinic lights). Control keys used are described in detail in Appendix 2.

  • Page 75: Appendix 4 - O Electrode Calibration

    APPENDIX 4 – O ELECTRODE CALIBRATION The O probe calibration is done outside of the photobioreactor software and data recorded by the software (in mV) has to be firs exported and then converted to units of interest using the calibration constants determined during calibration process described below.

  • Page 76: Appendix 5 - Co Electrode Calibration

    APPENDIX 5 – CO ELECTRODE CALIBRATION Prior the start of the experiment it is important to perform the CO electrode calibration. Please follow the instruction provided by the electrode manufacturer (www.mt.com ) and instruction described below. There are three different ways one can use to calibrate the CO electrode for use in PBR FMT150: 1.
  • Page 77 Fig. 56 A) Typical calibration curve for CO electrode. B) Conversion tables. Modified from www.mt.com. CHEMICAL SOLUTION REQUIRED FOR THE CALIBRATION  Electrolyte solution as supplied with the membrane kit (Ordering No. 51 340 015).  Deionized water for preparing standard solutions. ...
  • Page 78  When not in use, the CO electrode should always be kept immersed in the storage solution.  In all calibration and sample solutions the ionic strength should be the same, and should not exceed a maximum value of 1.0 mol/l. During the calibration the pH value should be less than 4.0. This is achieved by adding HCl to the solution in the vessel during calibration resulting in release of all of the available from the calibration solution.
  • Page 79 and extra precaution has to be taken to maintain the water CO free). Allow the N gas to escape through one of the ports like the waste port (keep it open during bubbling with N ). In the meantime prepare additional 50 ml of DIC water in falcon tube and bubble it through with N .
  • Page 80 the vessel with volume of 465 ml results in final concentration 20 µM of dissolved CO (dCO ). If concentration of 100 µM dCO is required 5 ml of calibration solution should be added to the vessel with volume of 465 ml. ...
  • Page 81  Finally click on the Set CO fit to confirm the CO sensor calibration values. mV values will be converted into the read-out of µM dCO  The frequency of recalibration operations depends on the degree of accuracy required and on the measuring conditions.

  • Page 82: Appendix 6 - Conversion Table For Co Calibration

    APPENDIX 6 – CONVERSION TABLE FOR CO CALIBRATION Correction table for the volume of calibration solution to be added into the vessel for the calibration step in respect of maximum vessel volume Note: 1 ml of calibration solution added to the 400 ml cuvette with maximum volume of 465 ml results in final concentration of 20uM dCO2 in the vessel FNT150 400 ml FNT150 1000 ml ml DIC free H2O in cuvette...

  • Page 83: Appendix 7 - Gas 150 Technical Specifications

    APPENDIX 7 – GAS 150 TECHNICAL SPECIFICATIONS Warm-up time: 45 minutes Input/Output Connector: Parker Prestolock 6 mm Weight: 5 kg Dimensions: 41x37x17 cm Carbon Dioxide Module - Specification: Available CO ranges: 0-2 %, 0-3 %, 0-5 %, 0-10 %, and 0-20 % (please note that if any other than standard range is required, the customer needs to specify the required range) Standard Range: 0-2 % Accuracy at +25 °C (+77 °F) against certified factory references: <±[0.02% CO...

  • Page 84: Appendix 8 - Qy Measurement

    APPENDIX 8 – QY MEASUREMENT Below schematic description of the QY measurement as it is done in the PBR FMT150 is shown.

  • Page 85: Appendix 9 - Description And Vessel Assembly Of 3000 Ml Vessel

    APPENDIX 9 – DESCRIPTION AND VESSEL ASSEMBLY OF 3000 ML VESSEL VESSEL DESCRIPTION AND SETUP The cultivation vessel has a capacity of 3000 ml. It is made from flame-proof glass (thickness 3.3 mm), stainless steel (AISI316L), duralumin with black eloxal coating, silicone sealing, and Lukopren S6410T glue. Each vessel is tested and autoclaved (20 minutes at 120 °C) by the manufacturer before distribution.

  • Page 86: Technical Specification Standard 400Ml Pbr

    TECHNICAL SPECIFICATION STANDARD 400ML PBR Measured chlorophyll Fluorescence Parameters: Fo, Ft, Fm, Fm´, Fv/Fm. Other parameters can be calculated Vessel Capacity / Weight: 400 ml / 2.8 kg Precision Controlled Temperature: Standard: 15 °C - 55 °C Optional: 10 – 70 °C Temperature Sensor: Platinum Pt 1,000 in thermo-well Cooling and heating system:...

  • Page 87: Technical Specification Standard 1000Ml Pbr

    TECHNICAL SPECIFICATION STANDARD 1000ML PBR Measured chlorophyll Fluorescence Parameters: Fo, Ft, Fm, Fm´, Fv/Fm. Other parameters can be calculated Vessel Capacity / Weight: 1000 ml / 3.8 kg Precision Controlled Temperature: Standard: 15 °C - 55 °C Optional: 10 – 70 °C Temperature Sensor: Platinum Pt 1,000 in thermo-well Cooling and heating system:...

  • Page 88: Technical Specification Standard 3000Ml Pbr

    TECHNICAL SPECIFICATION STANDARD 3000ML PBR Measured chlorophyll Fluorescence Parameters: Fo, Ft, Fm, Fm´, Fv/Fm. Other parameters can be calculated Vessel Capacity / Weight: 3000 ml / 6.2 kg Precision Controlled Temperature: Standard: 15 °C - 55 °C Optional: 10 – 70 °C Temperature Sensor: Platinum Pt 1,000 in thermo-well Cooling and heating system:...

  • Page 89: Warranty Terms And Conditions

    WARRANTY TERMS AND CONDITIONS  This Limited Warranty applies only to the Photobioreactor FMT150. It is valid for one year from the date of shipment.  If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 90: Alphabetical Index

    ALPHABETICAL INDEX Keyword Page AUX1 Connector 22, 24 AUX2 Connector 22, 24 Bubble interruption valve sensor calibration sensor module 15, 35, 76 Connection scheme Continuous mode Control keys Control menu tree Control modes Cultivation vessel Data export Device pairing Experiment start 53, 56, 62 External light panel 18, 39...
  • Page 91 Measuring modes sensor calibration sensor module 14, 34, 75 OD calibration 28, 52, 63 Operating system 51, 64 Optical density(OD) 10, 55 Optional accessories Peristaltic Pump PP500 17, 43 pH sensor 14, 30, 31 pH sensor calibration Power outage Power supply 22, 24, 26 Pulse light control PWM pump...

  • Page 92: List Of Abbreviations

    LIST OF ABBREVIATIONS E – Actinic Illumination Irradiance , F’ – continuous fluorescence level - continuous fluorescence level of dark adapted sample – maximal fluorescence level F’ – maximal fluorescence level in light-adapted sample – variable fluorescence parameter F – F F’...

  • Page 93: Troubleshooting And Customer Support

    TROUBLESHOOTING AND CUSTOMER SUPPORT In case of troubles and for customer support, please, write to support@psi.czor contact your local distributor. Manual Version: 2015/03 © PSI (Photon Systems Instruments), spol. sr.o.

Table of Contents