1. Manuals
  2. Brands
  3. Photon Systems Instruments Manuals
  4. Laboratory Equipment
  5. FMT150
  6. Manual and user manual

Photon Systems Instruments FMT150 Manual And User Manual

Photobioreactor
Hide thumbs Also See for FMT150:
Table of Contents

Advertisement

Quick Links

Download this manual
Photobioreactor
FMT150
Manual and User's Guide
Please read this manual before operating this product
PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic
FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the FMT150 and is the answer not in the manual?

Questions and answers

Related Manuals for Photon Systems Instruments FMT150

Summary of Contents for Photon Systems Instruments FMT150

  • Page 1 Photobioreactor FMT150 Manual and User’s Guide Please read this manual before operating this product PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz...
  • Page 2 © PSI (Photon Systems Instruments), spol. s r. o. © PSI (Photon Systems Instruments), spol. s r. o. (hereinafter PSI), 2019 This document and its parts can be copied or provided to a third party only with the express permission of PSI.

  • Page 3: Table Of Contents

    © PSI (Photon Systems Instruments), spol. s r. o. TABLE OF CONTENTS Warnings and Safety Precautions ........................... 5 General Description ................................ 7 Device Description ................................8 Configurations and Versions..........................9 Standard Components ............................14 Control Unit Front Panel ............................16 Rear Panel For Version FMT150.1 ........................
  • Page 4 © PSI (Photon Systems Instruments), spol. s r. o. Cultivation Guide ................................60 Set-Up the Components ............................60 Set-Up the Experiment ............................60 Appendix ..................................62 FMT150 Control Menu Tree ..........................62 QY Measurement ..............................70 Technical Specification Standards ........................71 Warranty Terms and Conditions ...........................

  • Page 5: Warnings And Safety Precautions

    © PSI (Photon Systems Instruments), spol. s r. o. 1 WARNINGS AND SAFETY PRECAUTIONS PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE TURNING THE PHOTOBIOREACTOR ON: • Remove all packaging and transport protectors before connecting the Photobioreactor to the power supply.
  • Page 6 © PSI (Photon Systems Instruments), spol. s r. o. The Photobioreactor is considered Class 1M* LED Product. LED radiation may be harmful to eye. Avoid direct and strongly reflected exposure. Use protective glasses. *Class 1M: Laser and LED equipment that is safe for the naked eye under foreseeable conditions of operation.

  • Page 7: General Description

    In addition, up to 8 peristaltic pumps for different chemostat cultivation control can be linked to the Photobioreactor FMT150 for the highly precise control of cultivation conditions.

  • Page 8: Device Description

    3 DEVICE DESCRIPTION Standard version of the Photobioreactor FMT150 consists of a cultivation vessel with a sealable lid and a base box containing electronics circuitry, LED light panel (Fig. 1A) and other components essential for the optimal operation of the photobioreactor.

  • Page 9: Configurations And Versions

    FMT150 can be shipped with various configurations of vessel capacity, light quality, light intensity and thermoregulation. Numerous additional accessories such as various sensors can be supplied as described in chapter 5 on page 33. Please note that this manual describes operation of two FMT150 versions (Fig. 4) - older PBR FMT150.1 and new PBR FMT150.2.
  • Page 10 The high-power light emitting diodes generate a highly uniform irradiance flux that can be controlled in the range 0 – 3,000 mol.m PAR. The irradiance can be dynamically modulated by the FMT150 control unit or by the PBR software. Spectra are shown in Fig. 3.
  • Page 11 The FMT150 in both 400 and 1000 ml models have been upgraded from FMT150.1 (Fig. 4A) to FMT150.2 (Fig. 4B). This manual provides instructions for operation of both versions (original, now referred to FMT150.1 and the current version FMT150.2). Please always ensure that you refer to correct instructions.
  • Page 12 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 4 Two versions of the PBR FMT150: A) FMT150.1, B) FMT150.2...
  • Page 13 © PSI (Photon Systems Instruments), spol. s r. o. List of parameters measured and/or calculated by the Photobioreactor FMT150: Symbol Name Description (Dark) Instantaneous fluorescence in the Instantaneous F-yield in dark equals F dark (in dark-adapted sample) (Light) Instantaneous fluorescence in the...

  • Page 14: Standard Components

    PBR software no measured data can be stored and visualized as well as no user- defined protocols can be designed. Chapter 3.3 and chapter 7.1 describe how to operate the PBR FMT150 in cultivation mode via front panel control unit, without use of PBR software.
  • Page 15 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 5 Components of the Spare part kit. Numbers correspond to component number in Tab. 4. Component Component Description Specification Explanation Number Male Luer Integral Lock MTLL240- 5/32” (4.0 mm) J1A* For fixing of stainless tubes in the 5/16”...

  • Page 16: Control Unit Front Panel

    Please note that neither turbidostat nor chemostat module can be operated if FMT150 is purchased without the PBR software. The front panel of the FMT150 control unit has 4 LED lights on the left and 5 control keys on the right (Fig. 7).
  • Page 17 3.3.3 DISPLAY ON THE RIGHT This display shows various functions of the FMT150 and these are activated and set with the main keys on the right side of the control unit as described below. 3.3.4 MAIN KEYS •...

  • Page 18: Rear Panel For Version Fmt150.1

    3.4.2 CONNECTOR FOR OPTIONAL SENSOR sensor should be plugged to this connector when in use. PBR FMT150 control unit recognizes when the sensor is connected and will automatically control the operation of the sensor. Only O...
  • Page 19 USB connector with serial communication cable is provided as part of the PBR FMT150 package when purchased with the PBR software to connect the PBR FMT150 with the computer. This connection has to be made for the communication of the PBR FMT150 via the PBR software.

  • Page 20: Rear Panel For Version Fmt150.2

    Fig. 9 Photobioreactor control unit and its power supply rear panel. A) Back panel of the Photobioreactor control unit. B) Serial number on the front panel of the PBR FMT150 unit. C) Rear panel of the power supply with the serial number (blue arrow).
  • Page 21 IDENTIFICATION LABEL WITH SERIAL NUMBER Each Photobioreactor FMT150 has a serial number that corresponds with the serial number of the power supply unit. These are always provided together and are not interchangeable. Prior to switching PBR FMT150 device ON make sure that the serial number on the front panel of the main body matches the serial number on the power supply (Fig.

  • Page 22: Connection Schema

    Depending on the use of optional modules and the set-up of the PBR FMT150, the connection schema for the optional and standard modules with the PBR FMT150 control unit rear panel may differ. Please refer to Tab. 5 below to see the possible order of connecting the different modules to the PBR FMT rear panel.

  • Page 23: Device Installation

    © PSI (Photon Systems Instruments), spol. s r. o. 4 DEVICE INSTALLATION To install the device properly, it is necessary to follow the proper sequence of the assembly instructions. Instructions for assembly of standard PBR FMT150 (400, 1000 and 3000 ml) are described below. POWER SUPPLY •...

  • Page 24: Cultivation Vessel

    6.2 kg 31.70 x 7.36 x 30.3 cm Tab. 6 Weights and dimensions. Fig. 12 Schematic design of the PBR cultivation vessel types. A) Vessel for PBR FMT150/400. B) Vessel for PBR FMT150/1000. C) Vessel for PBR FMT150/3000. 4.2.1 SETUP The lid of the vessel is fastened to the vessel via two screws (n°3 in Fig.
  • Page 25 Sampling tube can be used as gas outlet when Gas Analysis System (GAS) is connected to the PBR FMT150; for more information see page 51. Once the cultivation vessel is set up and placed in the photobioreactor it should be locked to the body of the PBR FMT150 by the clips on each side of the PBR.
  • Page 26 FMT150, calibration constants stored in the system after OD calibration will no longer correspond to the new conditions. In 1000 ml or 3000 ml PBR FMT150 version glass tunnel is used to improve optical path for OD and fluorescence measurements, therefore any shift in the position in the vessel might slightly change the optical path of the measuring beam and influence the absolute measured values.
  • Page 27 4.2.4 3000 ML VESSEL SPECIFICS The PBR FMT150/3000 provides more connectors than in 400/1000 ml models to give the user broader range of connected optional instruments and larger scope of operational features. Please refer to Fig. 15 for detail description of the 3000ml vessel lid and all the connectors: •...

  • Page 28: Ph/Temperature Sensor

    (described in chapter 3.2) and is recommended for purchase if precise control of cultivation conditions is required. The module consists of electronic unit embedded in Photobioreactor FMT150 Control Unit, probe and communication cable (Fig. 21).
  • Page 29 Prior to initiation of each experiment it is recommended to perform the pH sensor calibration. The calibration can be done either in PBR software (for more details see PBR Software manual) or using the FMT150 control unit front panel as described below.
  • Page 30 © PSI (Photon Systems Instruments), spol. s r. o. Please note that the calibration solutions are not provided by the manufacturer. • Read the signal value on the display of the photobioreactor control unit (Fig. 17B), wait until it is stable and confirm this value by pressing the “S”...

  • Page 31: Gas Supply

    Fig. 18 Assembly of the bubble humidifier bottle. A) Components needed for the assembly of the bottle. B) Assembled lid. C) Assembled bubble humidifier bottle. • Connect the bubble interruption valve cable with the AUX2 connector on the back of the FMT150 or with the peristaltic pump via the AUX Out (see Fig. 10 on page 22). •...
  • Page 32 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 19 FMT150 set-up with air pump supplying the gas.

  • Page 33: Optional Accessories/Components

    5 OPTIONAL ACCESSORIES/COMPONENTS The functions of the PBR FMT150 may be enhanced by wide range of optional components. Installation and operation of the optional accessories that can be provided together with the PBR FMT150 device are described in next chapters.

  • Page 34: Ph/Temperature Module

    To measure pH and temperature. It is required for cultivation thermoregulation. The module consists of electronic unit embedded in Photobioreactor FMT150 Control Unit, sensor probe and probe cable (Fig. 21). InPro3253SG/120/PT1000 or InPro3253/120/PT1000 single rod measuring cells from METTLER TOLEDO (www.mt.com) are supplied as low maintenance, autoclavable and pressure resistant pH electrodes with a factory filled pre-pressurised liquid electrolyte and a reference electrolyte together with a silver ions barrier.
  • Page 35 D) Attaching the cable to the rear side of the PBR FMT150.2. • When connected to the PBR FMT150 control unit, the sensor is automatically recognized. The function of the O sensor can be controlled manually by the front panel of the control unit or by PBR software. For manual control please refer to chapter 7.1 on page 62 for more detailed explanation.

  • Page 36: Co 2 Module

    The CO module is designed for continuous monitoring of dissolved carbon dioxide concentration in the medium. The module consists of external electronic unit for connection with Photobioreactor FMT150 Control Unit, sensor probe and sensor communication cable (Fig. 24). InPro5000/12/120 sensor from METTLER TOLEDO (www.mt.com) with integrated temperature probe is used for potentiometric CO concentration measurement.
  • Page 37 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 25 Assembly of the CO electrode. • Insert the CO electrode into the appropriate vessel connector and secure it tightly (Fig. 26A). Note: The position of the sensor in the lid is optional. In case other sensors are used, we recommend...
  • Page 38 © PSI (Photon Systems Instruments), spol. s r. o. The calibration of the CO probe can be performed with assistance of PBR software (for more details see PBR Software manual). Please follow also the instruction provided by the electrode manufacturer (www.mt.com) and instruction described below.
  • Page 39 © PSI (Photon Systems Instruments), spol. s r. o. Chemical solution • Electrolyte solution as supplied with the CO membrane kit. • Deionized water for preparing standard solutions. • 32% HCl for pH adjustment. • Standard 20 µM solution of NaHCO and Na (78.24 mg of NaHCO...
  • Page 40 • Switch ON the PBR FMT150 and place the empty vessel in its holding place. Install the lid of the vessel with the sensor and other ports closed off. Ensure the stirring bar is placed inside the vessel and the magnetic stirrer is installed on the outside of the vessel.
  • Page 41 © PSI (Photon Systems Instruments), spol. s r. o. Important: Please monitor the pH of the solution over the time course of the calibration to ensure that it remains at 4 or lower. Add more HCl into the solution if pH increases above 4. At pH above 4 will be converted back into bicarbonate and will no longer be measured by the probe (Fig.
  • Page 42 © PSI (Photon Systems Instruments), spol. s r. o. Bubbled gas Temp (µM) (mg.l 400 ppm CO 25 °C 13.6 5,000 ppm CO 25 °C 7.48 10,000 ppm CO 25 °C 14.96 15,000 ppm CO 25 °C 22.44 Tab. 7 CO calibration example.

  • Page 43: Magnetic Stirrer

    Fig. 30C whereas the older versions (Fig. 30A, B) were controlled by the PBR. Fig. 30 Magnetic stirrer for A) FMT150.1 version (two different stirrer types: slow and high speed stirrer). B) FMT150.2 version (slow and high speed stirrer).
  • Page 44 Fig. 31 Relation between rotation speed in rpm and PWM value in % adjusted by the PBR. A) refers to slow-speed and high-speed magnetic stirrer version for PBR FMT150.1 or 150.2. Maximum speed rotation is indicated on the stirrer body. B) refers to the current magnetic stirrer (600 rpm) version for PBR FMT150.2.
  • Page 45 For setting via PBR control software refer to Photobioreactor software manual. Fig. 33 Screws for fixing the stirrer to the front side of the PBR main body. A) FMT150.1 version (slow-speed rotation or high-speed rotation). B) FMT150.2 version (slow-speed of rotation or high-speed of rotation). C) Current version of magnetic stirrer for PBR...

  • Page 46: Pwm Pump

    PBR FMT150.2 version with own control unit (speed of rotation 600 rpm). Fig. 35 A, B, C) Attaching of the communication cable to the “AUX 1” and “AUX IN” (A – FMT150.1 version; B, C – FMT150.2 version). D, E) Manual Stirrer control via the PWM_Mode on the front panel of PBR (D – FMT150.1 version; E – FMT150.2 version). F) Horizontal position of the service button.
  • Page 47 PWM Pump when connected to the PBR has to be set for REMOTE MODE operation to be directly controlled and operated via Remote Control software for PBR FMT150. This pump can also be used in a manual mode as a stand- alone instrument.

  • Page 48: Gas Mixing System Gms 150

    © PSI (Photon Systems Instruments), spol. s r. o. • Connect the short silicone tube to the IN port on the rear panel of the PWM Pump. (Note: This improves supply of homogeneous gas flow to the pump). Connect the silicone tubing between the OUT port on the PWM Pump rear panel with the humidifier bottle (Fig.
  • Page 49 See Fig. 39C. This configuration will prevent a flooding of the gas mixing system caused by water recoil when sparging is stopped. • When the GM System is connected to the PBR FMT150 the vessel lid assembly scheme (Fig. 39B) is same as for standard air installation as described on page 26.
  • Page 50 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 39 GM System connection with the PBR FMT150. A) Mixed gas of given composition (1) is pumped from GM System via (2) to the PBR FMT150 via air interruption valve (3). B) Vessel lid assembly. (4) Air distribution tube, (7) Medium outlet, (6) Sampling tube.

  • Page 51: Gas Analysis System Gas 150

    5.9.1 INSTALLATION Follow the instructions below to connect the Gas Analysis System 150 (GAS 150) with the PBR FMT150: • Connect the GAS 150 with the AC power cord which is part of the accessory GAS 150 package (Fig. 42A) and plug to electrical outlet.
  • Page 52 Numbers correspond to: (1) GMS 150 output; (2) GAS 150 input for mixed air from GMS 150; (3) GAS output for bringing the mixed air to humidifier bottle (4) and to the PBR FMT150. (5) Input for gas from the cultivation vessel to GAS 150. (6) Air from the vessel passes through dehumidifier prior CO concentration analysis.
  • Page 53 Fig. 44 Vessel lid assembly when GAS 150 system module is connected to PBR FMT150. A) Lid assembly wheb peristaltic pumps are connected to PBR FMT150 in parallel with GAS 150 and standard aeration U-tube is used. B) Top view of vessel lid when peristaltic pumps are connected to PBR FMT150 in parallel with GAS 150.

  • Page 54: Peristaltic Pump Pp600

    PBR software is required to use the pump in turbidostat or chemostat mode. Note: the operation of the peristaltic pump cannot be controlled by the front panel of the PBR FMT150 control unit. Without the PBR software, the operation of peristaltic pump is controlled solely by the Peristaltic pump Control display.
  • Page 55 © PSI (Photon Systems Instruments), spol. s r. o. Important note: the pumps connected to the FMT150 must have unique IDs, otherwise the behavior of pumps is unpredictable. Turbidostat mode – the Photobioreactor optical sensing unit monitors the optical density (OD680, OD720/OD735) of the culture.
  • Page 56 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 47 Assembly of the peristaltic pump. A, B) Short tube is attached around the rotor wheel to the peristaltic pump. C, D) Adapter is attached to the silicone tubing and connected with the tubing of the peristaltic pump. E) Tubing from the peristaltic pump is connected with the cultivation vessel.
  • Page 57 Fig. 48 Final assembly of the peristaltic pump. A) Second luer connector is attached. B) Connection of the silicone tubing with the medium for the cultivation. C) Fully installed peristaltic pump tubing. D) Schematic representation of PBR FMT150 unit connected to peristaltic pumps in chemostat and turbidostat mode and to GMS 150.

  • Page 58: External Light Panel

    Position the Light Source by inserting its pivot joints into the respective slots in the holders – see Fig. 50B. • Fig. 50C shows the proper position of the Light Source. Fig. 50 Correct positioning of the external light panel and the final set-up of the PBR FMT150 with the external light source.

  • Page 59: Additional Software Licence

    © PSI (Photon Systems Instruments), spol. s r. o. The function of the external light is controlled manually by the front panel of the PBR control unit or by PBR software. For manual operation via front panel please refer to chapter 7.1 on page 62.

  • Page 60: Cultivation Guide

    © PSI (Photon Systems Instruments), spol. s r. o. 6 CULTIVATION GUIDE This section describes show to set up a standard Photobioreactor FMT150 and how to initiate cultivation of an algal culture with a PBR software. SET-UP THE COMPONENTS 1. First prepare the glass cultivation vessel and its lid. Follow the steps described in chapter 4.2.3 on page 26 to assemble the vessel.
  • Page 61 (provided with the system) for the OD calibration to avoid possible interference of external light. OD calibration can be performed manually on the front panel of the FMT150 control unit or by PBR software. Please refer to chapter 7.1 on page 62 for manual control or to the PBR Software manual for calibration via software.

  • Page 62: Appendix

    7 APPENDIX FMT150 CONTROL MENU TREE This section explains the operation of the Photobioreactor FMT150 control. Photobioreactor can operate in cultivation mode by setting the cultivations parameters such as light, temperature, etc. in the menu navigation tree. Please note that even though various parameters such as chlorophyll fluorescence and OD, temperature, etc.
  • Page 63 © PSI (Photon Systems Instruments), spol. s r. o. 7.1.2 OPERATION SCHEMATIC...
  • Page 64 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 65 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 66 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 67 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 68 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 69 Control keys used are described in detail in Appendix on page 62. Note: Please note that the Pulse Light Control protocol can be operated only via front panel of the PBR FMT150 control unit and is currently not supported in PBR software.

  • Page 70: Qy Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. QY MEASUREMENT Below is shown schematic description of the QY measurement. Fig. 52 QY measurement theory.

  • Page 71: Technical Specification Standards

    . Other LED colors available as an option. OD – Optical Density Measurement: Real time measurement of OD by two LEDs (735 nm for version FMT150.1, 720 nm for version FMT150.2, 680 nm for both FMT 150.1 and FMT150.2) Detector Wavelength Range: PIN photodiode with 665 nm –...
  • Page 72 . Other LED colors available as an option. OD – Optical Density Measurement: Real time measurement of OD by two LEDs (735 nm for version FMT150.1, 720 nm for version FMT150.2, 680 nm for both FMT 150.1 and FMT150.2) Detector Wavelength Range: PIN photodiode with 665 nm –...
  • Page 73 © PSI (Photon Systems Instruments), spol. s r. o. 7.3.3 FMT150 3000 ML Measured chlorophyll Fluorescence Parameters: Fo, Ft, Fm, Fm´, Fv/Fm. Other parameters can be calculated. Vessel Capacity / Weight: 3000 ml / 6.2 kg Precision Controlled Temperature: Standard (ambient temperature around 23 °C): 10 – 75 °C...
  • Page 74 © PSI (Photon Systems Instruments), spol. s r. o. 7.3.4 GAS 150 Warm-up time: 45 minutes Input/Output Connector: Parker Prestolock 6 mm Weight: 5 kg Dimensions: 41 x 37 x 17 cm Carbon Dioxide Module – Specification: Available CO ranges: 0 – 2 %, 0 – 3 %, 0 – 5 %, 0 – 10 %, and 0 – 20 % (please note that if any other than standard range is required, the customer needs to specify the required range) Standard Range: 0 –...

  • Page 75: Warranty Terms And Conditions

    © PSI (Photon Systems Instruments), spol. s r. o. 8 WARRANTY TERMS AND CONDITIONS • This Limited Warranty applies only to the Photobioreactor FMT150 device. It is valid for one year from the date of shipment. • If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 76: Troubleshooting And Customer Support

    © PSI (Photon Systems Instruments), spol. s r. o. 9 TROUBLESHOOTING AND CUSTOMER SUPPORT In case of troubles and for customer support, please, visit on our websites, write to support@psi.cz or contact your local distributor.

  • Page 77: List Of Abreviations

    © PSI (Photon Systems Instruments), spol. s r. o. 10 LIST OF ABREVIATIONS E – Actinic Illumination Irradiance , F’ – continuous fluorescence level - continuous fluorescence level of dark adapted sample – maximal fluorescence level F’ – maximal fluorescence level in light-adapted sample variable fluorescence parameter F –...

Table of Contents