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Photon Systems Instruments Plant Pen Instruction Manual

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3225246+96+66+66252001

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  • Page 1 3225246+96+66+66252001...
  • Page 2 Manual Version: 2025/01 © PSI (Photon Systems Instruments), spol. s r.o. www.psi.cz This document and its parts can be copied or provided to a third party only with the express permission of PSI. The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.

  • Page 3: Table Of Contents

    ABLE OF ONTENT Information before using N-Pen device ....................5 General description ..........................6 Technical Specification ..........................7 Device description ..........................9 List of equipment and customer information ....................9 Care and maintenance ..........................9 Principle of measurement ........................10 Leaf Optical Properties and Nitrogen ......................
  • Page 4 10.1 GPS/N-Pen operation ..........................30 10.2 Data download ............................31 Warranty terms and conditions ......................32 Troubleshooting and customer support ....................32 List of figures ............................33 Page | 4...

  • Page 5: Information Before Using N-Pen Device

    NFORMATION BEFORE USING EN DEVICE Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide. Always follow corresponding manuals while working with the N-Pen device or doing the maintenance.

  • Page 6: General Description

    ENERAL DESCRIPTION N-Pen N 110 is a light-weight, battery-powered reflectance-based instrument that provides a convenient, cost-effective method for effective nitrogen management in plants throughout their growing season. Essentially, the N-Pen characterizes nitrogen amount by means of reflectance and by the concept of a close link between chlorophyll content and nitrogen content in plants. Rugged and compact N-Pen can be used for plant biology research or for education.

  • Page 7: Technical Specification

    2.1 T ECHNICAL PECIFICATION N-Pen Normalized Difference Greenness Index NDGI = (R780 - R560) / (R780 + R560) - Correlated with NDGI Protocols Nitrogen content - Measured in percentage Calibration for maize, wheat and barley LED emitter 565 nm a 760 nm PIN photodiode with bandpass filters Detector From 500 to 800 nm...
  • Page 8 Bluetooth module compliance data Category Country Standard FCC Part 15 Subpart B: 2008 Class B FCC CRF Title 47 Part 15 Subpart C FCC ID: T9J-RN42 ETSI EN 301 489-1 V1.8.1 Radio Europe ETSI EN 301 489-17 V2.1.1 ETSI EN 300 328 V1.7.1 Canada IC RSS-210 low power comm.

  • Page 9: Device Description

    EVICE DESCRIPTION Fig. 1 Device description. 3.1 L IST OF EQUIPMENT AND CUSTOMER INFORMATION Carefully unpack the carton. You should have received the following items: • N-Pen N 110, • carrying case, • textile strap for comfortable wearing, • N-Pen Operating Manual (on a USB flash disc), •...

  • Page 10: Principle Of Measurement

    Li-ion battery • Avoid fully discharging of the battery. • Do not keep the battery at full charge for all the time. • Keeping at high temperatures shortens battery life. Regular service Regular servicing is essential to ensure reliable and long-term operation of the equipment. For optimal care, maintenance should be conducted by an authorized service center.

  • Page 11: N-Pen Configuration

    were correlated with N-content in experimental plant groups. Pearson’s correlation coefficient was used as a statistical measure of the strength of linear relationship between the paired data (Fig. 3A). The highest correlation was found for reflectance in the ranges 530 – 630 nm and 700 –...

  • Page 12: Nitrogen Nutrition

    Fig. 4 Ne-Pen configuration. 4.3 N ITROGEN UTRITION The outcome of the measurement with N-pen is formulated as relative N-content in plant dry matter (%). Another practical interpretation of the NDGI value, is the N nutrition state (Fig. 5). The maximum NDGI value is set to 100 % and the minimum reflectance is set equal to 0 % as follows: 1.

  • Page 13: Getting Started

    ETTING STARTED The PlantPen device is powered with built in Li-Ion battery. Before use, make sure the battery is fully charged. You can charge the PlantPen by connecting it to a PC using a USB cable or to an AC outlet via a USB adapter (not included) and the provided cable. Define the number of samples per each experimental group.

  • Page 14: Control Menu Tree

    ONTROL MENU TREE The next few pages of this manual show the structure of the menu and explain in a schematic way the operation of the N-Pen. The schematic diagrams show the Main Menu, first-level Sub-Menus and second-level Sub-Menus. • The blue color represents the Main Menu and its Options.
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  • Page 17: Usb Connection

    7 USB C ONNECTION N-Pen comes with the USB cable that is required for charging of the Li-ion battery and can also be used for data transfer to the PC after completion of measurements. To connect the USB cable with the N-Pen device follow the picture instructions below. Please note that a lock in system is used to secure the USB cable to the N-Pen and extreme caution has to be used when setting up this connection to avoid damage to the cable pins.

  • Page 18: Bluetooth Connection

    LUETOOTH CONNECTION In addition to data transfer via USB the N-Pen may be connected to the software via Bluetooth for data transfer. Before setting up the Bluetooth connection between the N-Pen and the PC, ensure the following components are in place: Bluetooth hardware installed on the computer The PC must support Bluetooth wireless technology, either through integrated hardware or a Bluetooth card.
  • Page 19 Fig. 8 Starting the Bluetooth application – Windows 11. Fig. 9 Windows 11 – Advanced Bluetooth devices discovery. Page | 19...
  • Page 20 Adding a Bluetooth device to the computer • Click “Add a device” to begin searching for new Bluetooth device (Fig. 10, Fig. 11). • Ensure the N-Pen is set to discoverable mode before proceeding. • Select: PSI N-Pen icon. • Click: Next (Fig.
  • Page 21 Starting the Pairing Process The steps to start pairing differ between the older and newer versions of the N-Pen, as they are equipped with different Bluetooth modules. Old version of N-Pen N 100: The Bluetooth Pairing Code is 0000. • Select: “Enter the device’s pairing code”.
  • Page 22 Completing the N-Pen Pairing • Click: Close (Fig. 15) to finish the Bluetooth pairing process. The Bluetooth pairing is now complete, and the next step is to open the FluorPen software (included on the delivered USB flash disk). For detailed information on using the FluorPen software, refer to Chapter 9. Fig.

  • Page 23: Fluorpen Software

    LUOR EN SOFTWARE 9.1 S OFTWARE INSTALLATION 1. Copy the FluorPen software provided on the USB flash disk to your computer and launch the FluorPen program. 2. To connect and recognize the N-Pen device in the FluorPen software, proceeds first with the registration of the FluorPen software (Fig.

  • Page 24: Menu And Icons Explanation

    9.2 M ENU AND ICONS EXPLANATION 9.2.1 M AIN MENU MENU: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format. Export to JSON Exports data in JavaScript Object Notation. Close Closes the current experiment.

  • Page 25: Menu Settings

    9.2.2 M ENU SETTINGS MENU > Setup > Settings (Fig. 18) After Download – Memory Erase If the box is checked the N-Pen memory is erased after each data download. Data – Inverted If the box is checked the polarity of data is inverted, e.g., multiplied by -1. This feature can be helpful for a certain type of experiment when the measured data are undesirably interpreted as negative values.

  • Page 26: Data Download And Export

    Online Control – Time MENU: Device > Online Control > Time (Fig. 20) The window allows you to configure the N-Pen’s time and date settings. Manual Adjustment: You can manually set the time and date by entering the desired values and saving them. Automatic Synchronization: To synchronize the N-Pen’s clock with your computer, tick the synchronization box.

  • Page 27: Firmware Update

    4. To export data from the FluorPen software select File > Export or Export icon. Select data type to export, see Fig. 22. Selected only – exports only one measurement that is selected by mouse, otherwise it will export everything. Source data –...
  • Page 28 2. Warning: Select OK to start update (Fig. 24). Fig. 24 Warning. 3. Selecting .bxn file Find firmware update file (a binary file with the extension .bxn provided from PSI) and select Open (Fig. 25). Fig. 25 Selection of the firmware file. 4.
  • Page 29 The bottom bar indicates the upload progress (Fig. 27). Fig. 27 Firmware upload progress. Press OK to finish the upload (Fig. 28). Fig. 28 Firmware upload finishing. Page | 29...

  • Page 30: Gps Module

    10 GPS MODULE The new versions of the N-Pen device 110 have integrated GPS module which can be turned on during the measurements. When GPS module is turned on the map coordinates will be automatically saved with all collected data and will be downloaded during data download.

  • Page 31: Data Download

    10.2 D ATA DOWNLOAD 1. Enabling Communication: • Switch on the computer and the FluorPen software. • Enable Bluetooth or connect the device with computer via communication cable (see instructions on pg. 17). 2. Downloading Data from the N-Pen: • Start FluorPen program.

  • Page 32: Warranty Terms And Conditions

    11 W ARRANTY TERMS AND CONDITIONS • This Limited Warranty applies only to the N-Pen device. It is valid for one year from the date of shipment. • If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 33: List Of Figures

    13 L IST OF FIGURES Fig. 1 Device description....................................9 Fig. 2 Absorption and reflectance spectra..............................10 Fig. 3 Correlation between N content in barley dry matter and reflectance at particular wavelengths indicated as Pearsons correlation coefficient along the reflectance spectrum (A). Relationship between NDGI index and N content in barley dry matter (b) can be interpreted as a sigmoid curve.

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