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Photon Systems Instruments AquaPen-C AP 110-P Manual And User Manual

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AquaPen-C AP 110-C
AquaPen-P AP 110-P
Manual and User Guide
Please read this manual before operating this product
PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic
FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz

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  • Page 1 AquaPen-C AP 110-C AquaPen-P AP 110-P Manual and User Guide Please read this manual before operating this product PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz...
  • Page 2 © PSI (Photon Systems Instruments), spol. s r. o. © PSI (Photon Systems Instruments), spol. s r. o. (hereinafter PSI), 2018 This document and its parts can be copied or provided to a third party only with the express permission of PSI.
  • Page 3 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 4: Table Of Contents

    © PSI (Photon Systems Instruments), spol. s r. o. TABLE OF CONTENTS Information Before Using AquaPen Device ........................6 Technical Specification ..............................7 General Information ............................... 8 Device Description ..............................9 List of Equipment and Customer Information ......................10 Care and Maintenance ..............................11 Principle of Measurement ............................
  • Page 5 © PSI (Photon Systems Instruments), spol. s r. o. 11.4 Firmware Update ..............................50 GPS Module ................................52 12.1 GPS / AquaPen Operation ........................... 52 12.2 Data Download ..............................53 Warranty Terms and Conditions ..........................54 Troubleshooting and Customer Support ......................... 55...

  • Page 6: Information Before Using Aquapen Device

    © PSI (Photon Systems Instruments), spol. s r. o. 1 INFORMATION BEFORE USING AQUAPEN DEVICE Read this manual carefully before operating the device. If you are not sure about anything in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide.

  • Page 7: Technical Specification

    © PSI (Photon Systems Instruments), spol. s r. o. 2 TECHNICAL SPECIFICATION Measured / Calculated Parameters: ‘, QY, NPQ 1, NPQ 2, NPQ 3, OJIP, LC 1, LC 2, LC 3, OD *, OD Saturating Pulse Illumination: Adjustable from 10 to 100 % (up to 3,000 µmol.m Actinic Illumination: Adjustable from 10 to 1,000 µmol.m...

  • Page 8: General Information

    © PSI (Photon Systems Instruments), spol. s r. o. 3 GENERAL INFORMATION AquaPen is a lightweight, hand-held fluorometer intended for quick and reliable measurements of photosynthetic activity in algal, cyanobacterial or eventually plant cell suspensions. The photosynthetic activity is derived from the chlorophyll fluorescence (ChlF) kinetics.

  • Page 9: Device Description

    © PSI (Photon Systems Instruments), spol. s r. o. DEVICE DESCRIPTION Fig. 1 Device description.

  • Page 10: List Of Equipment And Customer Information

    © PSI (Photon Systems Instruments), spol. s r. o. 4 LIST OF EQUIPMENT AND CUSTOMER INFORMATION Standard version of the AquaPen device package consists: • AquaPen-C AP 110-C or AquaPen-P AP 110-P • Carrying Case • 4 ml volume plastic cuvette with stopper (AquaPen-C only) •...

  • Page 11: Care And Maintenance

    © PSI (Photon Systems Instruments), spol. s r. o. 5 CARE AND MAINTENANCE AquaPen-P AP 110-P • Never submerge the whole device in the measured sample! • Keep in mind that only the optical tip can be submerged! • Rinse the optical tip of the AquaPen-P in freshwater after each use.

  • Page 12: Principle Of Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 6 PRINCIPLE OF MEASUREMENT AquaPen is a new version of the FluorPen fluorometer which can measure different parameters in solutions of algae, cyanobacteria and plant cell suspensions at very low concentrations. It is equipped with a blue and red LED emitter (Fig.
  • Page 13 © PSI (Photon Systems Instruments), spol. s r. o. Measuring the optical density of growing cultures is a common method to quantify various important culture parameters like cell concentration, biomass production or changes in the cell morphology. Continuous measuring of optical density is the most basic and powerful tool for providing optimal yields.
  • Page 14 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 3 AquaPen-C AP 110-C. Fig. 4 AquaPen-P 110-P.

  • Page 15: How To Get Started

    © PSI (Photon Systems Instruments), spol. s r. o. 7 HOW TO GET STARTED For more detailed information on particular steps of AquaPen operation please refer to chapter 8. The device works on Li-Ion battery or is charged via USB cable from PC or grid.
  • Page 16 © PSI (Photon Systems Instruments), spol. s r. o. Setting of optimal intensities of pulses: Flash pulse setting The optimum value of Flash pulse you can identify with QY measurement. Before QY measurement is recommended to set the pulse color according to measured culture and intensity of Super pulse on 70 %.

  • Page 17: Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 6 OJIP measurement performed with different intensities of Super pulse. Actinic pulse setting Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application.
  • Page 18 © PSI (Photon Systems Instruments), spol. s r. o. • AquaPen measure required parameter. If you choose protocol such as OJIP, LC or NPQ the display shows the percentage of measurements. • Value of required parameter appears on display after measuring. Result of OJIP, NPQ or LC protocol is a graph, so all data you will see after data download using FluorPen Software.

  • Page 19: Ojip Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.3 OJIP PROTOCOL The AquaPen device offers the possibility to capture rapid fluorescence transient – OJIP, which occurs during exposure of photosynthesizing organisms to high irradiance. The FluorPen software enables data downloading to a personal computer and subsequent OJIP analysis.

  • Page 20: Non-Photochemical Quenching (Npq) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.4 NON-PHOTOCHEMICAL QUENCHING (NPQ) PROTOCOL The NPQ protocol is the most typically used measuring approach to quantify photochemical and non-photochemical quenching. The measurement should be performed with a dark-adapted sample. Thereby, it may not be appropriate under field conditions.
  • Page 21 © PSI (Photon Systems Instruments), spol. s r. o. L - indicates light adapted parameters; D - refers to dark recovery phase after switching of the actinic illumination; n - represents a sequential number of light phase; ss - steady state Calculated as (F –...
  • Page 22 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 7 NPQ Protocol.

  • Page 23: Light Curve (Lc) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.5 LIGHT CURVE (LC) PROTOCOL The protocol called Light Curve (LC) was designed to acquire parameters for construction of Light Response Curve relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the sample exposed to a stepwise increase of light intensity.
  • Page 24 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 8 LC1 Protocol.
  • Page 25 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 9 LC2 Protocol.

  • Page 26: Optical Density Measurement (Aquapen-C Only)

    © PSI (Photon Systems Instruments), spol. s r. o. OPTICAL DENSITY MEAS UREMENT (AQUAPEN -C ONLY) 7.2.1 CALIBRATION Blank – either water or ideally medium used to cultivate the culture you plan to measure in AquaPen. Calibration assures you that your measurements are accurate within the specification limits that led you to select the instrument in the first place.

  • Page 27: Multiple Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. • Go to Measurement > OD680nm or OD720nm. • Press SET to confirm. • Value of required parameter appears on display after measuring. Measured data are stored into the device memory and can be downloaded to PC (page 47).

  • Page 28: Control Menu Tree

    © PSI (Photon Systems Instruments), spol. s r. o. 8 CONTROL MENU TREE The next pages show the structure of the operation scheme, which includes the Main Menu, first-level Sub-Menus and second-level Sub-Menus. • The blue color represents the Main Menu and its Options.
  • Page 29 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 30 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 31 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 32 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 33 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 34 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 35 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 36: Usb Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 9 USB CONNECTION Connect the USB cable with the AquaPen device. Please note that lock in system is used. Pay attention when connecting the USB cable not to damage the outlet connector on the Pen device.

  • Page 37: Bluetooth Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 10 BLUETOOTH CONNECTION Before you set up the Bluetooth connection between the AquaPen and your PC, make sure you have these components: 1. Bluetooth enabled PC The PC with which you connect must have Bluetooth wireless technology, either built-in or through a Bluetooth card. Make sure that the PC's Bluetooth setting is "discoverable"...
  • Page 38 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 11 Start Bluetooth Application. 3. Opening the Add Bluetooth Device Application • Select: “Add a device” to start searching new Bluetooth device. Be sure that the AquaPen is in discoverable mode (see step 1).
  • Page 39 © PSI (Photon Systems Instruments), spol. s r. o. 5. Starting the Pairing Process This step is different for old and new version of Bluetooth module. Old version: Your Bluetooth Pairing Code is: 0000 • Select: “Enter the device’s pairing code”.
  • Page 40 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 16 Finishing. On computer run the program FluorPen 1.1. For more information about FluorPen software see chapter 11.

  • Page 41: Fluorpen Software

    © PSI (Photon Systems Instruments), spol. s r. o. 11 FLUORPEN SOFTWARE 11.1 SOFT WARE INSTALLATIO N 1. Save the FluorPen software provided on the USB flash disk to your computer and launch the FluorPen program. 2. To connect and recognize your AquaPen device in the FluorPen software proceeds first with the registration of your FluorPen software (Fig.

  • Page 42: Menu And Icon Explanation

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 18 Connecting AquaPen with Software. 11.2 MENU AND ICON EXPLAN ATION 11.2.1 MAIN MENU MENU: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format.

  • Page 43: Menu Settings

    © PSI (Photon Systems Instruments), spol. s r. o. MENU: Setup Device ID Detects the connected device. Update Firmware Used for firmware updates. Settings Used for modification of the program settings. MENU: Help About Offers basic information about the program.

  • Page 44: Menu Online Control

    © PSI (Photon Systems Instruments), spol. s r. o. Data – GPS Visible This option is active only in older AquaPen version AP 100. In new version AP 110 the GPS data are automatically downloaded and paired with protocol measurements.
  • Page 45 © PSI (Photon Systems Instruments), spol. s r. o. Online Control – Time Set the AquaPen time a date. You can also synchronize time of AquaPen device with computer time. Online Control – Protocols Choose the protocol for measuring. AquaPen device start to measure. After measuring you can download data.
  • Page 46 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 47: Data Transfer And Visualization

    © PSI (Photon Systems Instruments), spol. s r. o. 11.3 DATA TRANSFER AND VI SUALIZATION 1. Perform a measurement with your AquaPen. In Fig. 19 we did an OJIP measurement. 2. Click the Download icon or select Device > Download.
  • Page 48 © PSI (Photon Systems Instruments), spol. s r. o. 4. To visualize measurement in the graphic mode, click the Graph field in the bottom bar. 5. The colored Graph of measured data appears (Fig. 20). Fig. 20 Graphic visualization of experiment.
  • Page 49 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 21 Export data.

  • Page 50: Firmware Update

    © PSI (Photon Systems Instruments), spol. s r. o. 11.4 FIRMWARE UPDATE All data in the AquaPen memory are erased during the firmware update! Before starting any firmware update, export all your data from the AquaPen memory into your computer! 1.
  • Page 51 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 24 Select .bxn file. 4. Finishing Upload • Select: OK to start uploading of the update (Fig. 25). Fig. 25 Data loss warning. • The bottom bar indicates the upload progress (Fig. 26).

  • Page 52: Gps Module

    © PSI (Photon Systems Instruments), spol. s r. o. 12 GPS MODULE AquaPen device has integrated GPS module which can be turned on during the measurement and the GPS coordinated will be added to the downloaded data. For proper GPS reading, the time in your AquaPen and in your computer must be synchronized. Preset time and time zone must correspond to GPS time (time zone) in your location.

  • Page 53: Data Download

    © PSI (Photon Systems Instruments), spol. s r. o. 12.2 DATA DOWNLOAD 1. Enabling Communication: • Switch on the computer and the AquaPen. Set your computer to AquaPen communication: enable Bluetooth or connect to USB port. 2. Downloading Data from the AquaPen •...

  • Page 54: Warranty Terms And Conditions

    © PSI (Photon Systems Instruments), spol. s r. o. 13 WARRANTY TERMS AND CONDITIONS • This Limited Warranty applies only to the AquaPen device. It is valid for one year from the date of shipment. • If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 55: Troubleshooting And Customer Support

    © PSI (Photon Systems Instruments), spol. s r. o. 14 TROUBLESHOOTING AND CUSTOMER SUPPORT In case of troubles and for customer support, please, visit on our websites, write to support@psi.cz or contact your local distributor.

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