Photon Systems Instruments FluorPen FP 110 Manual And User Manual
  1. Manuals
  2. Brands
  3. Photon Systems Instruments Manuals
  4. Measuring Instruments
  5. FluorPen FP 110
  6. Manual and user manual

Photon Systems Instruments FluorPen FP 110 Manual And User Manual

Monitoring pen
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
FluorPen FP 110
PAR-FluorPen FP 110
Monitoring Pen MP 100
Manual and User Guide
Please read this manual before operating this product
PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic
FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the FluorPen FP 110 and is the answer not in the manual?

Questions and answers

Related Manuals for Photon Systems Instruments FluorPen FP 110

Summary of Contents for Photon Systems Instruments FluorPen FP 110

  • Page 1 FluorPen FP 110 PAR-FluorPen FP 110 Monitoring Pen MP 100 Manual and User Guide Please read this manual before operating this product PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz...
  • Page 2 © PSI (Photon Systems Instruments), spol. s r. o. © PSI (Photon Systems Instruments), spol. s r. o. (hereinafter PSI), Oct 2018 This document and its parts can be copied or provided to a third party only with the express permission of PSI.

  • Page 3: Table Of Contents

    © PSI (Photon Systems Instruments), spol. s r. o. TABLE OF CONTENTS Information Before Using FluorPen Device ........................5 Technical Specification ..............................6 General Information ............................... 8 Device description ............................... 11 List of equipment ................................12 Care and Maintenance ..............................13 Principle of Measurement ............................
  • Page 4 © PSI (Photon Systems Instruments), spol. s r. o. 12.2 Data Download ..............................51 Warranty Terms and Conditions ..........................52 Troubleshooting and Customer Support ........................53 Appendix ..................................54 15.1 Battery Pack for Monitoring Pen ......................... 54 15.1.1 Standard Battery Pack ..........................54 15.1.2...

  • Page 5: Information Before Using Fluorpen Device

    © PSI (Photon Systems Instruments), spol. s r. o. 1 INFORMATION BEFORE USING FLUORPEN DEVICE Read this manual carefully before operating the device. If you are not sure about anything in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide.

  • Page 6: Technical Specification

    © PSI (Photon Systems Instruments), spol. s r. o. 2 TECHNICAL SPECIFICATION PAR-FluorPen & FluorPen Protocols - Ft – instantaneous chlorophyll fluorescence - Quantum Yield - OJIP PAR-FluorPen FP 110 - Non-photochemical quenching - Light curve - Photosynthetically Active Radiation (measured as PPFD) - Ft –...
  • Page 7 © PSI (Photon Systems Instruments), spol. s r. o. Monitoring Pen Protocols - Ft – instantaneous chlorophyll fluorescence - Quantum Yield - OJIP - Non-photochemical quenching - Light curve LED lighting LED emitter Blue (455 nm), other wavelength on request Saturating pulse Illumination Up to 3,000 µmol(photon).m...

  • Page 8: General Information

    The “X“ version is mounted with custom-made leaf-clip. PAR-FluorPen FP 110/S (Fig. 1a) Same features as the standard FluorPen FP 110/S plus Photosynthetically Active Radiation (PAR) meter in the range from 400 to 700 nm. Equipped with a standard leaf-clip.
  • Page 9 © PSI (Photon Systems Instruments), spol. s r. o. PAR-FluorPen FP 110/X Same features as the PAR-FluorPen FP 110/D but the “X“ version is mounted with custom-made leaf-clip. Fig. 1a) PAR-FluorPen FP 110/S. b) PAR-FluorPen FP 110/D. c) FluorPen FP 110/P.
  • Page 10 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 2a) Monitoring Pen MP 100-E. b) Monitoring Pen MP 100-A.

  • Page 11: Device Description

    © PSI (Photon Systems Instruments), spol. s r. o. 3. 1 DE V ICE DE S CRI P TI ON Fig. 3 Device description.

  • Page 12: List Of Equipment

    © PSI (Photon Systems Instruments), spol. s r. o. 4 LIST OF EQUIPMENT Carefully unpack the carton. You should have received the following items: • FluorPen/Monitoring Pen • Carrying Case • Textile Strap for Comfortable Wearing • FluorPen Operating Manual (on a USB flash disc) •...

  • Page 13: Care And Maintenance

    © PSI (Photon Systems Instruments), spol. s r. o. 5 CARE AND MAINTENANCE FluorPen and Monitoring Pen • Never submerge the device in water! (except Monitoring Pen MP 100-A) • The device should not come in contact with any organic solvents, strong acids or bases.

  • Page 14: Principle Of Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 6 PRINCIPLE OF MEASUREMENT FluorPen is a chlorophyll fluorometer and is used to measure different photosynthetic parameters in plants. It is equipped with a blue LED emitter (455 nm), optically filtered and precisely focused to deliver light intensities of up to 3,000 µmol.m to measured plant tissue (Fig.
  • Page 15 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 5 Chlorophyll fluorescence. Parameters measured by the FluorPen: - Instantaneous Chlorophyll Fluorescence is equivalent to F if the sample is dark-adapted. QY - Quantum Yield QY is a measure of the Photosystem II efficiency. QY is equivalent to F in dark-adapted samples and to F light-adapted samples.

  • Page 16: Getting Started

    © PSI (Photon Systems Instruments), spol. s r. o. 7 GETTING STARTED For more detailed information on particular steps of FluorPen operation please refer to chapter 8. The device can is powered with built in Li-Ion battery. Ensure the battery is fully charged by plugging it into a PC via USB cable or the AC outlet via the USB cable and a USB adaptor (not included).
  • Page 17 © PSI (Photon Systems Instruments), spol. s r. o. Setting the optimal intensities of pulses: Flash Pulse setting The optimum value of Flash pulse can be determined with QY measurement as shown in Fig.6 below. Before performing QY measurement it is recommended to set the intensity of Super pulse to 80 %.
  • Page 18 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 7 OJIP measurement performed with different intensities of Super pulse. Fig. 8 OJIP data - measurement performed with different intensities of Super pulse. The highest Fv/Fm value indicates the optimal intensity of Super pulse (20% in this case).

  • Page 19: Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. If Overflow is observed on the display during measurement please lower the intensity of used pulses. In case of Low value on display during measurement please increase the intensity of used pulses.
  • Page 20 © PSI (Photon Systems Instruments), spol. s r. o. The OJIP protocol includes the following measured and calculated parameters: Abbreviation Explanation Bckg Background , fluorescence intensity at 50 µs 50µs = fluorescence intensity at J-step (at 2 ms) = fluorescence intensity at i-step (at 30 ms)

  • Page 21: Non-Photochemical Quenching (Npq) Protocols

    © PSI (Photon Systems Instruments), spol. s r. o. 7.3.2 NON-PHOTOCHEMICAL QUENCHING (NPQ) PROTOCOLS The NPQ protocol is used to quantify photochemical and non-photochemical quenching. It should be performed with dark- adapted samples. The NPQ protocol starts with measurement of minimal level of fluorescence F during a dark period.
  • Page 22 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 9 NPQ Protocol.

  • Page 23: Light Curve (Lc) Protocols

    © PSI (Photon Systems Instruments), spol. s r. o. 7.3.3 LIGHT CURVE (LC) PROTOCOLS The protocols called Light Curve (LC) were designed to acquire parameters for construction of Light Response Curve relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the sample exposed to a stepwise increase of light intensity.
  • Page 24 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 10 LC1 Protocol.
  • Page 25 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 11 LC2 Protocol.

  • Page 26: Multiple Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 7. 4 M UL T IP LE M E A S URE M E NT In addition to a single measurement with each of the available protocols, it is possible to perform multiple measurements of the same protocol over a period of time.

  • Page 27: Control Menu Tree

    © PSI (Photon Systems Instruments), spol. s r. o. 8 CONTROL MENU TREE The next few pages of this manual show the structure of the menu and explain in a schematic way the operation of the Fluor Pen. The schematic diagrams show the Main Menu, first-level Sub-Menus and second-level Sub-Menus.
  • Page 28 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 29 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 30 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 31 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 32 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 33 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 34 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 35: Usb Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 9 USB CONNECTION All FluorPens come with USB cable that is required for charging of the Li battery and can be also used for data transfer. To connect the USB cable with the FluorPen device Follow the picture instructions below. Please note that a lock-in system is used to secure the USB cable to the FluorPen and extreme caution has to be used when setting up the connection.

  • Page 36: Bluetooth Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 10 BLUETOOTH CONNECTION In addition to data transfer via USB the FluorPen may be connected to the software via Bluetooth. Before setting up the Bluetooth connection between the FluorPen and the PC, ensure the following components are in place: 1.
  • Page 37 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 13 Start Bluetooth Application. 3. Opening the Add Bluetooth Device Application • Select: “Add a device” to start searching for the new Bluetooth device. Be sure that the FluorPen is in discoverable mode (see step 1).
  • Page 38 © PSI (Photon Systems Instruments), spol. s r. o. 5. Starting the Pairing Process This step is different for old (FP100) and new version (FP110) of the FluorPen. The old version FP100: The Bluetooth Pairing Code is: 0000 • Select: “Enter the device’s pairing code”.
  • Page 39 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 18 Finishing. The Bluetooth pairing is now complete and the next step is to open the Fluor Pen 1.1 software (included on the USB flash disk). For more information about FluorPen software see chapter 11.

  • Page 40: Fluorpen Software

    © PSI (Photon Systems Instruments), spol. s r. o. 11 FLUORPEN SOFTWARE 11 .1 SO F TW A RE IN S T AL L AT IO N 1. Copy the FluorPen software provided on the USB flash disk to your computer and launches the FluorPen program.

  • Page 41: Menu And Icon Explanation

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 20 Connecting FluorPen device with Software. 11 .2 M E NU AN D IC ON E XP L A NA TI ON 11.2.1 MAIN MENU MENU: File Load Loads previously saved data files.

  • Page 42: Menu Settings

    © PSI (Photon Systems Instruments), spol. s r. o. MENU: Setup Device ID Detects the connected device. Update Firmware Used for firmware updates. Settings Used for modification of the program settings. MENU: Help About Offers basic information about the program.

  • Page 43: Menu Online Control

    © PSI (Photon Systems Instruments), spol. s r. o. Data – GPS Visible This option is active only in older version of the FluorPen - FP 100 and Monitoring Pen MP 100. In new versions of FP 110 the GPS data are automatically downloaded and paired with protocol measurements.
  • Page 44 PC at a later time. Measuring of PAR is active only in the PAR-FluorPen FP 110 (see picture below). Also, Bby pressing the Multi button in this window the Multiple...

  • Page 45: Data Transfer And Visualization

    © PSI (Photon Systems Instruments), spol. s r. o. 11 .3 D AT A TR AN S FE R AN D VI SU A LI ZA T IO N 1. Once kinetics protocol data (OJIP, NPQ, LC) has been collected by the FluorPen it needs to be downloaded to PC to be visualized.
  • Page 46 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 21 Example of Data Transfer and Visualization. 4. To visualize the data in the graph mode, click the Graph field in the bottom bar. 5. The selected set of data will be shown on the graph (Fig. 22).

  • Page 47: Firmware Update

    © PSI (Photon Systems Instruments), spol. s r. o. 6. To export data from FluorPen software select File > Export or Export icon. Select the type of data to export (Ft, QY, OJIP…) (Fig. 23). Selected only – exports only one measurement that is selected by mouse, otherwise it will export everything.
  • Page 48 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 24 Update Firmware. 2. Warning • Select: OK to start update (Fig. 25). Fig. 25 Warning. 3. Selecting .bxn file • Find firmware update file: Binary file (with the extension .bxn) (Fig. 26).
  • Page 49 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 26 Select .bxn file. 4. Finishing Upload • Select: OK to start uploading of the update (Fig. 27). Fig. 27 Data loss warning. • The bottom bar indicates the upload progress (Fig. 28).

  • Page 50: Gps Module

    © PSI (Photon Systems Instruments), spol. s r. o. 12 GPS MODULE All new versions of the FluorPen devices FP110 have integrated GPS module which may be turned on during the measurement for mapping of the collected data to specific filed position. When GPS module is turned on the map coordinates will be automatically saved with all collected data and will be downloaded during data download.

  • Page 51: Data Download

    © PSI (Photon Systems Instruments), spol. s r. o. 12 .2 D AT A D O WN LO A D 1. Enabling Communication: • Switch on the computer and the FluorPen device. Set the computer to FluorPen communication: enable Bluetooth or connect to USB port (see instructions above in chapter 9 and 10).

  • Page 52: Warranty Terms And Conditions

    © PSI (Photon Systems Instruments), spol. s r. o. WARRANTY TERMS AND CONDITIONS • This Limited Warranty applies only to the FluorPen device. It is valid for one year from the date of shipment. • If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 53: Troubleshooting And Customer Support

    © PSI (Photon Systems Instruments), spol. s r. o. 14 TROUBLESHOOTING AND CUSTOMER SUPPORT In case of troubles and for customer support, please, visit on our websites, write to support@psi.cz or contact your local distributor.

  • Page 54: Appendix

    © PSI (Photon Systems Instruments), spol. s r. o. 15 APPENDIX 15 .1 B AT TE R Y P AC K FO R M O NIT OR IN G PE N Battery pack serves as an external power source for Monitoring Pen devices. The external battery provides power during long-term experiments.

  • Page 55: Extended Temperature Range Battery Pack

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 33 Connectors of Standard Battery Pack. Replacement of the Battery: If the battery needs to be changed follow these steps: 1. Unscrew 4 screws (in each corner of the battery casing) and remove the battery pack cover (Fig. 34a).
  • Page 56 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 35 Monitoring Pen MP 100-A charged via Extended Temperature Range Battery Pack. Connectors of Battery Pack: Device - provides power connection between Monitoring Pen and the battery. This connection is necessary for Pen operation and data download.
  • Page 57 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 36 Battery replacement for Extended Temperature Range Battery Pack.

  • Page 58: Installation And Operation Of The Monitoring Pen Mp 100-A/B

    © PSI (Photon Systems Instruments), spol. s r. o. 15 .2 IN ST A LL A TI ON AN D O PE RA TI ON O F T HE M O NI T OR ING PE N M P 1 00 - A/B 15.2.1...

  • Page 59: Device Installation

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 38 Battery status. The minimal battery voltage is 4.9 V. The device does not work at lower voltage. Please find more information about the battery pack in chapter 15.1. 15.2.2 DEVICE INSTALLATION 1.

  • Page 60: Detachable Leaf Clips

    © PSI (Photon Systems Instruments), spol. s r. o. 15 .3 DE T A C HA B LE LE AF CL I PS Detachable leaf clips are used with the FluoPen FP 110/D and PAR-FluorPen FP110/D for dark adaptation of the leaf before measurements of chlorophyll fluorescence.

This manual is also suitable for:

Par-fluorpen fp 110Mp 100

Table of Contents