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Slee Cromatec I Operating Manual

Flexible staining systems with highest throughput
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O P E R A T I N G
M A N U A L
S T A I N I N G S Y S T E M S
C r o m a t e c I / I I
FLEXIBLE STAINING SYSTEMS
WITH HIGHEST THROUGHPUT
D ES I GN &
M A N UFACT UR I NG
M A DE IN GE R M A NY

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Summary of Contents for Slee Cromatec I

  • Page 1 O P E R A T I N G M A N U A L S T A I N I N G S Y S T E M S C r o m a t e c I / I I FLEXIBLE STAINING SYSTEMS WITH HIGHEST THROUGHPUT D ES I GN &...
  • Page 2 Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 3: Table Of Contents

    1.1.1 Used symbols and their meanings 1.2 Intended use 1.3 Authorized operators 1.4 Instrument type 1.5 Safety Instructions for working with the Cromatec I / II and reagents 2 Components of the Cromatec linear stainer 2.1 Instrument components 2.2 Standard components 2.3 Technical specification...
  • Page 4 4.6 Settings 4.6.1 Setting of Cromatec 4.6.2 Agitation / Fan 4.6.3 Filter 4.6.4 Options 4.6.5 Language 4.6.6 Time and date 4.6.7 Display 4.6.8 Service 4.7 Running staining program 4.7.1 Place slide baskets in the loading station 4.7.2 Select the program you want to stain 4.7.3 Start the program 4.7.4 Symbols during program 4.7.5 End of program...

  • Page 5: Introduction

    Introduction About this Manual This manual shall help you handling the device. Please read the manual completely and follow the advice. Acquaint yourself to the control elements and their functionality by studying the figures. This way, an adequate use of the device is ensured. 1.1.1 Used symbols and their meanings Danger warning: Danger warnings are marked by a red framed triangle.

  • Page 6: Intended Use

    Intended Use The Cromatec I / II is an automated stainer and is made for the purpose of staining tissue specimens in histology and pathology laboratories only. The instrument may be operated only according to the instructions contained in this manual. Any other use of the Cromatec I / II is considered improper.

  • Page 7: Safety Instructions For Working With The Cromatec I / Ii And Reagents

    Read the manual carefully before working with the Cromatec I / II and reagents for the first time. Keep the manual near and easily accessible to the device. Consult the manual in case of obscurities.
  • Page 8 If foreign objects have entered the device, switch off the device immediately and contact the technical service department or a local representative of SLEE medical GmbH.

  • Page 9: Components Of The Cromatec Linear Stainer

    Components of the Cromatec I / II linear stainer Instrument Components Touch screen display Handle Loading station for 5 slide baskets Unloading station for 5 slide baskets Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 10 Water drain receptacle Water drain washing cuvettes Water connection / inlet Connection power cable Network connection RJ45 USB connector type B Hose connection exhaust air Ø 100 mm Water flow pressure regulator Pressure gauge Emergency stop button Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 11: Standard Components

    Standard components Cromatec I Art.-No.: Description Quantity 41000566 Cover plate 2 pcs 41000017 Plastic cuvettes normal 20 pcs 41000410 Plastic washing cuvettes complete 6 pcs 41000584 Insert tub assembled 1 pcs 41000580 Drip tray with lifting plate 1 pcs 41000270...

  • Page 12: Technical Specification

    Technical Specification General Nominal Voltage 100 – 240 V AC +/- 10 % Nominal frequency 50 / 60 Hz Max. power consumption 350 VA Max. heat emission 350 J/s IEC 1010 classification Protective class 1 Pollution degree 2 Overvoltage installation category II Fuses 2 x T 3,15 A Interfaces...

  • Page 13: Setting Up The Instrument

    Setting up the instrument Installation site requirements – first time Requirements for the installation site: • The device may only be used indoors. • Stable, precisely horizontal laboratory table with a uniform surface of 1.80 m and a depth of 0.80 m, which can hold a weight of 120 kg! •...

  • Page 14: Tap Water Supply Connection

    Tap water supply connection • Unpack the water inlet hose (including safety-aqua stop). • Connect the hose to the inlet connection. Always connect the water inlet hose! Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 15: Waste Water Hose Connection

    Waste water hose connection • Connect the waste hose to the waste water connection. Please note that water can escape in the upper drain hose, but in the lower only in an exceptional case. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 16 Please do not bend the hoses and ensure an adequate slope. The two drains on the rear of the unit must never be brought together. The lower drain is to be used only for cleaning purposes to rinse and clean the cuvette trough.

  • Page 17: Electrical Connection

    Make sure to use the appropriate mains cable for the local voltage supply. Connection of air evacuation (optional) • For the connection to an external air evacuation please contact the local Slee service. Inserting / Changing the activated carbon filter The Cromatec will be delivered with the filter already installed.

  • Page 18: Levelling The Instrument

    Leveling the instrument • Once all accessories are installed move the instrument to its final position. • The easiest way to find the right level is using a spirit level. • The right level will be achieved by adjusting the instrument feet. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 19: Inserting The Cuvettes

    Inserting the cuvettes • There are three different cuvette types: Reagent cuvettes Running water cuvettes Heating cuvette / drying station (only Cromatec II) The reagent cuvette can simply be placed at the any station. Reagent cuvette The washing cuvette can simply be clicked into the water tube at the button of the station.
  • Page 20 Only Cromatec II: Inserting the heating cuvette / drying station Heating cuvette / drying Connection of heating station cuvette / drying station The heating cuvette is only available once in the device, as a heating cuvette / drying station in station 1, or as a heating cuvette / drying station in station 20. Only the plastic cuvette in station 1 or 20 is replaced by the heating cuvette or drying station.

  • Page 21: Inserting The Slide Baskets

    Inserting the slide baskets • The slide basket carrier system consists of two parts: The slide basket for 30 slides The basket carrier to connect to the magnet system To make sure the slide basket is not falling out of the transport system, you can hold it with your finger as shown in the picture.

  • Page 22: Setting The Water Pressure For Running Water Station

    3.10 Setting the water pressure for running water station The red marking indicates the maximum flow pressure during operation. 3.11 Store samples in xylene at the end of the process For this purpose, an insert tray with bow handle and lid was specially developed so that the samples can be stored in xylene at the end of the process.

  • Page 23: Operation

    Operation Switching on the instrument Switch on the device at the main switch at the rear panel of the instrument. Main menu After switching on the instrument for the first time, the system starts booting and the following display is shown: From this status all functions are started.
  • Page 24 After switching on the instrument for the first time, the system starts booting and the following display is shown: Please follow the below instructions to save a new user. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 25 Please press “ADMIN” and enter with password the below instructions to save a new user. With the magnifier you can change the values of the user. If you want to add a user please press “New”. Press “Change” behind Short name to enter the Short name of the user. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 26 Please confirm with ok and to return to menu user details. Press change behind Name, Function to enter the Name and Function or Department of the user. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 27 Please confirm with ok and to return to menu user details. Press change behind Password to change the password. If <none> is selected, there is no password active. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 28 Please confirm with ok and to return to menu user details. Please confirm everything with save and to return to menu user selection. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 29 With the arrow you can return to the main menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 30 Please note that in this case, the last user is the actual user. If you want to change from user to administrator please follow the instructions in chapter 4.4.1. The following described changes can only be done through an Administrator. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 31: Edit A New Or Existing Program

    Programs The following instructions (4.3.1 until 4.5.1) can only be done by the Administrator! 4.3.1 Edit a new or existing program Please follow the below instructions to save a new program or to edit an existing program. Please press “Programs” to find the list of programs or to select a new one. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 32 With the magnifier you can edit an existing program. With the button you can program a new one. After pressing “New” in the “program selection” menu you see the following: While pressing “Change” behind Short name you can insert the requested details (Short name is the name of the program to be seen in the overview): Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 33 Please confirm every step with the “OK” button and to return to the menu “Program details”. While pressing “Change” behind Name you can insert the requested details (Name is the long version of the name of the program to be seen in the overview): Please confirm every step with the “OK”...
  • Page 34 While pressing “Change” behind Drip-off time you can select the time the basket may take out of the last cuvette for dripping of the reagent. You can select from 0 – 250. Please confirm every step with the “OK” button and to return to the menu “program details”.
  • Page 35 While pressing “Steps” behind Total duration you can select the step time and reagent. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 36 Please press “Change” behind reagent to select the reagent out of the below list. Please press “Change” behind Duration to select the incubation time. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 37 Please confirm your selection by pressing the OK button. Please repeat the step, described under 4.3.1 for every step (bath). Please note that the cromatec II can only work step by step. No station can be shipped. If a station has no reagent it has to be marked as empty. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 38: Edit A New Or Existing User

    Users 4.4.1 Edit a new or existing user Please follow the below instructions to save a new user or to edit an existing user. If you want to add or select a new user please press “Users” in main menu. With the magnifier you can change the values of the user.
  • Page 39 Press “Change” behind Short name to enter the Short name of the user. Please confirm with ok and to return to menu user details. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 40 Press change behind Name, Function to enter the Name and Function or Department of the user. Please confirm with ok and to return to menu user details. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 41 Press change behind Password to change the password. If <none> is selected, there is no password active. Please confirm with ok and to return to menu user details. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 42 Please confirm everything with save and to return to menu user selection. With the arrow you can return to the main menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 43: Edit Or Create A New Reagent

    Reagents 4.5.1 Edit or create a new reagent Please follow the below instructions to save a new reagent or to edit an existing reagent If you want to add or select a new reagent please press “Reagents” in main menu. With the magnifier you can adjust an existing reagent or with “New”...
  • Page 44 Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 45 RMS stands for Reagent Management and shows visually on the main screen to what extent a reagent has already been used. Furthermore, the upper and maximum uses can be defined. When the reagent has been replaced, simply press the "Replace" button and the counter starts again.
  • Page 46 Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 47 Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 48 4.5.2 Edit / select hot air station – only Cromatec II Please note that the option Hot air station is preinstalled (if option has been choosed) and no changes can be done in the reagent details. Drying station can only be selected as station 1 or 20. The agitation is then switched off. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 49: Settings

    Settings 4.6.1 Settings of the Cromatec Please follow the below instructions to edit and save settings. If you want to change the settings of the Cromatec please press “Settings” in main menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 50: Agitation/Fan

    4.6.2 Agitation/Fan To change the settings for agitation and fan please select “Agitation/Fan” in the Settings menu. The agitation and fan speed can be changed in the menu. You can select between slow, normal and fast. Please confirm your settings by pushing “Set”. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 51: Filter

    4.6.3 Filter To change the settings for the filter please select “Filter” in the Settings menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 52 If you want to change the filter usage time please select “Change”. Please adjust the values as follows. A value of 1000 hrs is default setting. You can select values from 1h to 9999 hrs. Please confirm your settings by pressing “OK”. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 53 If you have replaced the filter, please press “Replace”. The value shown under “Used” will be set to 0. (For filter exchange please refer to chapter 3.6) Please save your settings by pressing “Save”. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 54: Options

    4.6.4 Options – only Cromatec II To change the settings for the temperature of hot air station please select “Options” in the Settings menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 55 The hot-air station temperature can be selected between 30°C and 70°C. Please set the temperature and confirm with OK. The melting of the paraffin on the samples is dependent on the temperature, number of glass slides in the slide baskets, and sample thickness. In addition, the heating cuvette needs a certain amount of time to warm up, which is automatically controlled by a small waiting time via the software.
  • Page 56 The Bluetooth function can also be switched on and off here. Here you also can set the Bluetooth connection from ON to OFF. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 57: Language

    4.6.5 Language To change the settings for the language options please select “Language” in the Settings menu. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 58: Time And Date

    4.6.6 Time and date To change the settings for time and date please select “Clock” in the Settings menu. With + and – you can select the actual time and date. If you want to change the format of date please press “Change”. Possible formats are: DD.MM.YY and MM.DD.YY. With adjust you can select differences between summer and wintertime.

  • Page 59: Display

    4.6.7 Display To change the settings for the display please select “Display” in the Settings menu. You can select the display brightness from 0 to 10. With the button “Key click” you switch on / off the key tones. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 60 Please confirm your selection with set. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 61: Service

    4.6.8 Service To change the Service settings please select “Service” in the Settings menu. Service settings are password protected! Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 62: Place Slide Baskets In The Loading Station

    Run a staining program 4.7.1 Place slide baskets in the loading station Please put the slide baskets you want to stain into the loading station. You can place up to 5 slide baskets into the loading station. 4.7.2 Select the program you want to stain You can select the program you want to stain by pressing the current program.
  • Page 63 You will be taken to the Program Selection menu. Just click on the short name to choose the program. Once selected you will be back at the overview screen and the selected program is visible. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 64: Start The Program

    4.7.3 Start the program You start the program by pressing the “Start” button. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 65 Sample program – in this case PAP staining: Bath 1 - 3 30 seconds reagent Bath 4 15 seconds water Bath 5 3 minutes reagent Bath 6 1 minute water Bath 7 20 seconds reagent Bath 8: 30 seconds reagent Bath 9: 1 minute reagent...
  • Page 66 Program with hot air station: Hot air station temperature to cold (blue): Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 67 Hot air station temperature to warm (red): Hot air station error (black): Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 68 Hot air station set temperature reached (orange): Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 69: Symbols During Program

    4.7.4 Symbols during program In mains operation, the battery is showed in grey until it is fully charged. Then the icon disappears. In floating operation, the battery icon shows the level, dark blue with sufficient residual charge and red below 25%. When switching off (in the buffer mode) a signal tone sounds.

  • Page 70: End Of Program

    4.7.5 End of program After the program is finished, slide baskets are placed in the unload station (capacity 5 slide baskets) and the instrument indicates by a ton signal. In case the unload station is full, the instrument gives the following warning message: Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 71: Stop A Running Program

    4.7.6 Stop a running program If you want to stop a running program, press the “Stop” button. The following message will appear. Press “OK” and the program will stop. You have to take out the slide baskets manually. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 72: Emergency Stop

    4.7.7 Emergency stop In case of an emergency, the operation function of the Cromatec can be switched off by pressing the emergency stop located at the lower front of the instrument. To continue the program, release the emergency stop button and confirm the alarm message.

  • Page 73: Errors

    Errors Notes Any failure occurring during the operation of the Cromatec is indicated by an alarm sound and message, shown on the display. Please refer to the trouble shooting list. PopUp messages in normal operation After pressing STOP: Red PopUp “Would really cancel...
  • Page 74 Flow of the filter running time: Yellow PopUp Filter usage time expired, please replace. „INFO“ [OK] Expiration of reagent: Yellow PopUp „INFO” At least one reagent expired, please replace. [OK] At start attempt despite emergency stop: Resolve problem, unlock Yellow PopUp EMERGENCY HALT and select: “EMERGENCY HALT”...
  • Page 75 On start with expired filter running time: Filter usage time expired please replace. Yellow PopUp [OK] “INFO” (Start nevertheless possible) At least one reagent expired, please replace. When starting with reagents that have [OK] expired: Yellow PopUp (Start nevertheless possible) “INFO”...

  • Page 76: Error Messages

    Error messages in normal operation – only Cromatec II Staining procedures interrupted since hh:mm:ss [Retry] [Cancel] Motor Error: Red PopUp Messages: Overcurrent horizontal “MOTOR ERROR” Overcurrent vertical Timeout horizontal Timeout vertical Specimen transport impossible Manually remove specimen and call service Failure of the magnetic control: Red PopUp “MAGNET ERROR”...
  • Page 77 Specimen lost (while lifting down): Specimen lost at n1 n2 n3 (station no.) Red PopUp Correct, then touch OK. „ERROR“ [OK] Specimen sticking at n1 n2 n3 (station no.) Specimen holder stick or lost (combination Specimen lost at n4 n5 n6 (station no.) 8+9): Red PopUp Correct, then touch OK.

  • Page 78: Power Failure

    Power failure – only Cromatec II The Cromatec has a built-in battery to allow the unit to work even in a case of power failure. In case of a power failure the display shows the following symbol: The operation is continued using the emergency power supply. As the battery gets loaded during operation, a fully loaded device can be operated using the battery.

  • Page 79: Hood

    Hood When the hood is opened, the instrument stops immediately and the following Red PopUp window is shown. The cromatec II will not perform any staining program while the lid is open. When the lid is opened, the ventilation will automatically be increased. Manual_Cromatec_I_II_2021-10-007.00_EN.docx...

  • Page 80: Cleaning

    Cleaning General cleaning advice The Slee linear stainer Cromatec I / II has a solvent resistant surface area according to the requirements of histological laboratories so that an easy cleaning can be done without problems. Only the display should be cleaned with a damp cloth.

  • Page 81: Schedule Of Cleaning For Staining System

    Schedule of cleaning for staining system: _____________________ Date Staining Water bath Tube System Name troughs Manual_Cromatec_I_II_2021-10-007.00_EN.docx...
  • Page 82 Optimized H&E program Station Reagent Time Xylol 1,30 Isopropanol 1,30 Isopropanol 1,30 96% Ethanol 1,30 96% Ethanol 1,30 Water 1,30 Hemalum 1,30 Hemalum 1,30 Water 1,30 Glacial acetic acid 1,30 Running Water 1,30 Running Water 1,30 96% Ethanol 1,30 96% Ethanol 1,30 Eosin 1,30...
  • Page 83 Notes...
  • Page 84 SLEE medical GmbH • Am Neuberg 14 55268 Nieder-Olm • Germany www.slee.de T: +49 (0) 6136 76997-0 E: mail@slee.de www.slee.de YouTube LinkedIn Instagram INS5100GB ∙ Manual_Cromatec_I_II_2021-10-007.00_EN...

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