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Slee Cromatec I Operating Manual

Flexible staining systems with highest throughput
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O P E R A T I N G
M A N U A L
S T A I N I N G S Y S T E M S
C r o m a t e c I / I I
FLEXIBLE STAINING SYSTEMS
WITH HIGHEST THROUGHPUT
D ES I GN &
M A N UFACT UR I N G
M A D E I N GE R M A NY

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  • Page 1 O P E R A T I N G M A N U A L S T A I N I N G S Y S T E M S C r o m a t e c I / I I FLEXIBLE STAINING SYSTEMS WITH HIGHEST THROUGHPUT D ES I GN &...
  • Page 2 Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 3 The information, numerical data and notes contained in this document represent the current state of scientific knowledge and state-of-the-art technol- ogy as we understand it following thorough investigation in this field. SLEE medical GmbH is under no obligation to update the present manual periodi- cally and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc.

  • Page 4: Table Of Contents

    INTRODUCTION ______________________________________________________ 7 Used symbols and their meanings Intended use Authorized operators Device type Safety Instructions for working with the Cromatec I / II and reagents OVERVIEW DEVICE __________________________________________________ 11 COMPONENTS ______________________________________________________ 12 SPECIFICATIONS _____________________________________________________ 14 SETTING UP THE DEVICE _____________________________________________ 15 Installation site requirements –...
  • Page 5 ERRORS ____________________________________________________________ 58 Notes PopUp messages in normal operation Error messages in normal operation Power failure Hood CLEANING __________________________________________________________ 65 General cleaning advice Schedule of cleaning for staining system: Optimized H&E program SERVICE ____________________________________________________________ 68 WARRANTY _________________________________________________________ 68 DISPOSAL __________________________________________________________ 68 Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 6 Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 7: Introduction

    1 INTRODUCTION This manual shall help you handling the device. Please read the manual completely and follow the advice. Acquaint your- self to the control elements and their functionality by studying the figures. This way, an adequate use of the device is en- sured.

  • Page 8: Intended Use

    1.2 Intended use The Cromatec I / II is an automated stainer and is made for the purpose of staining tissue specimens in histology and pa- thology laboratories only. The device may be operated only according to the instructions contained in this manual. Any other use of the Cromatec I / II is considered improper.

  • Page 9: Safety Instructions For Working With The Cromatec I / Ii And Reagents

    When working with the Cromatec I / II, please pay particular attention to the used reagent’s characteristics regarding flam- mability and further hazards. Read the manual carefully before working with the Cromatec I / II and reagents for the first time.
  • Page 10 If foreign objects have entered the device, switch off the device immediately and contact the technical service department or a local representative of SLEE medical GmbH. Do not operate the device while wearing metal objects on the body.

  • Page 11: Overview Device

    2 OVERVIEW DEVICE Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 12: Components

    3 COMPONENTS The Slee staining system Cromatec I / II is provided with the following standard components: Cromatec I Item-No.: Description Quantity 41000566 Cover plate 2 pcs. 41000017 Plastic cuvettes normal 20 pcs. 41000410 Plastic washing cuvettes complete 6 pcs.
  • Page 13 41000558 Transport handles 4 pcs 41000782 Extension cable for drying station 1 pcs Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 14: Specifications

    4 SPECIFICATIONS General Nominal Voltage 100 – 240 V AC +/- 10 % Nominal frequency 50 / 60 Hz Max. power consumption 350 VA Max. heat emission 350 J/s IEC 1010 classification Protective class 1; Pollution degree 2; Overvoltage installation category II Fuses 2 x T 3,15 A Interfaces...

  • Page 15: Setting Up The Device

    SETTING UP THE DEVICE 5.1 Installation site requirements – first time Requirements for the installation site: • The device may only be used indoors. • Stable, precisely horizontal laboratory table with a uniform surface of 1.80 m and a depth of 0.80 m, which can hold a weight of 120 kg! •...

  • Page 16: Tap Water Supply Connection

    5.2 Tap water supply connection Unpack the water inlet hose (including safety-aqua stop). Connect the hose to the inlet connection. Always connect the water inlet hose! Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 17: Waste Water Hose Connection

    5.3 Waste water hose connection Connect the waste hose to the wastewater connection. Please note that water can escape in the upper drain hose, but in the lower only in an exceptional case. Please do not bend the hoses and ensure an adequate slope. The two drains on the rear of the unit must never be brought together.

  • Page 18: Electrical Connection

    Make sure to use the appropriate mains cable for the local voltage supply. 5.5 Connection of air evacuation (optional) For the connection to an external air evacuation please contact the local Slee service. 5.6 Inserting / Changing the activated carbon filter The Cromatec will be delivered with the filter already installed.

  • Page 19: Leveling The Device

    5.7 Leveling the device • Once all accessories are installed move the device to its final position. • The easiest way to find the right level is using a spirit level. • The right level will be achieved by adjusting the device feet. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 20: Inserting The Cuvettes

    5.8 Inserting the cuvettes There are three different cuvette types: • Reagent cuvettes • Running water cuvettes • Heating cuvette / drying station (only Cromatec II) Reagent cuvette The reagent cuvette can simply be placed at the any station. Washing cuvette The washing cuvette can simply be clicked into the water tube at the button of the station.

  • Page 21: Inserting The Heating Cuvette / Drying Station - Only Cromatec

    5.9 Inserting the heating cuvette / drying station - only Cromatec II Connection of heating cuvette / dry- Heating cuvette / drying station ing station The heating cuvette is only available once in the device, as a heating cuvette / drying station in station 1, or as a heating cuvette / drying station in station 20.

  • Page 22: Inserting The Slide Baskets

    5.10 Inserting the slide baskets The slide basket carrier system consists of two parts: • The slide basket for 30 slides • The basket carrier to connect to the magnet system basket carrier slide basket To make sure the slide basket is not falling out of the transport system, you can hold it with your finger as shown in the picture.

  • Page 23: Setting The Water Pressure For Running Water Station

    5.11 Setting the water pressure for running water station The red marking indicates the maximum flow pressure during operation. 5.12 Store samples in xylene at the end of the process For this purpose, an insert tray with bow handle and lid was specially developed so that the samples can be stored in xylene at the end of the process.

  • Page 24: Operation

    6 OPERATION 6.1 Switching on the device Switch on the device at the main switch at the rear panel of the device. 6.2 Main menu – home srceen After switching on the device for the first time, the system starts booting and the following display is shown: Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 25: Programs

    6.3 Programs Please press “Menu” on the home screen to enter the main menu. From this status all functions are started. The “Programs” button is for selection and changing of programs. The “Users” button is to set and change user data The “Reagents”...
  • Page 26 6.3.1 Edit a new or existing program Please follow the below instructions to save a new program or to edit an existing program. Please press “Programs” to find the list of programs or to select a new one. With the magnifier you can edit an existing program. With the button “new”...
  • Page 27 After pressing “New” in the “pro- gram selection” menu you see the following: While pressing “Change” behind “Shortname” you can insert the re- quested details (“Shortname” is the name of the program to be seen in the overview): Please confirm every step with the “OK”...
  • Page 28 While pressing “Change” behind “Name” you can insert the re- quested details (“Name” is the long version of the name of the program to be seen in the over- view): Please confirm every step with the “OK” button and to return to the menu “program details”.
  • Page 29 While pressing “Change” behind “Dripoff time” you can select the time the basket may take out of the last cuvette for dripping of the reagent. You can select from 0 – 250. Please confirm every step with the “OK” button and to return to the menu “program details”.
  • Page 30 While pressing “Steps” behind “Total duration” you can select the step time and reagent. Please press “Change” behind “Reagent” to select the reagent out of the below list. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 31 Please repeat the step for every step (bath). Please note that the Cromatec I / II can only work step by step. No station can be skipped. If a station has no reagent, it has to be marked as empty.

  • Page 32: Users

    6.4 Users 6.4.1 Edit a new or existing user Please follow the below instructions to save a new user or to edit an existing user. If you want to add or select a new user, please press “Users” in main menu.
  • Page 33 Please confirm with “OK” and to return to menu user details. Press “change” behind “Name, Function” to enter the Name and Function or Department of the user. Please confirm with “OK” to return to menu user details. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 34 Press “change” behind “Pass- word” to change the password. If <none> is selected, there is no password active. Please confirm with “OK” to return to menu user details. Please confirm everything with “save” and to return to menu user selection. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 35 With the arrow you can return to the main menu. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 36: Reagents

    6.5 Reagents 6.5.1 Edit or create a new reagent Please follow the below instructions to save a new reagent or to edit an existing reagent. If you want to add or select a new reagent, please press “Reagents” in main menu. With the magnifier you can adjust an existing reagent or with “New”...
  • Page 37 Enter “Shortname”, confirm with “OK”. Enter “Name”, confirm with “OK”. Choose “RMS” on or off. RMS stands for Reagent Management and shows visually on the main screen to what extent a reagent has already been used. Furthermore, the upper and maximum uses can be defined. When the reagent has been replaced, simply press the "Replace"...
  • Page 38 Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 39 6.5.2 4.5.2 Edit / select hot air station – only Cromatec II Please note that the option “Hot air station” is preinstalled (if option has been chosen) and no changes can be done in the reagent details. “Drying station” can only be selected as station 1 or 20. The agitation is then switched off. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 40: Settings

    6.6 Settings 6.6.1 Settings of the Cromatec Please follow the instructions below to edit and save settings. If you want to change the settings of the Cromatec please press “Set- tings” in main menu. 6.6.2 Agitation / Fan To change the settings for agitation and fan please select “Agitation / Fan” in the “Settings” menu. If you want to change the settings of the Cromatec please press “Set- tings”...
  • Page 41 6.6.3 Filter To change the settings for the filter please select “Filter” in the Set- tings menu. If you want to change the filter us- age time please select “Change”. Please adjust the values as follows. A value of 1000 hrs is default set- ting.
  • Page 42 If you have replaced the filter, please press “Replace”. The value shown under “Used” will be set to 0. For filter exchange please refer to the respective chapter. Please save your settings by press- ing “Save”. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 43 6.6.4 Options – only Cromatec II To change the settings for the tem- perature of hot air station please select “Options” in the Settings menu. The hot-air station temperature can be selected between 30 °C and 70 °C. Please set the temperature and confirm with “OK”.
  • Page 44 The melting of the paraffin on the samples is dependent on the temperature, number of glass slides in the slide baskets, and sample thickness. In addition, the heating cuvette needs a cer- tain amount of time to warm up, which is automatically controlled by a small waiting time via the software.
  • Page 45 6.6.5 4.6.5 Language To change the settings for the lan- guage options please select “Lan- guage” in the Settings menu. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 46: Time And Date

    6.6.6 Time and date To change the settings for time and date please select “Clock” in the Settings menu. With + and – you can select the ac- tual time and date. If you want to change the format of date, please press “Change”.
  • Page 47 6.6.7 Display To change the settings for the dis- play please select “Display” in the Settings menu. You can select the display bright- ness from 0 to 10. With the button “Key click” you switch on / off the key tones: Please confirm your selection with “Set”.
  • Page 48 6.6.8 Service To change the Service settings please select “Service” in the Set- tings menu. Service settings are password pro- tected! Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 49: Run A Staining Program

    6.7 Run a staining program 6.7.1 4.7.1 Place slide baskets in the loading station Please put the slide baskets you want to stain into the loading station. You can place up to 5 slide baskets into the loading station. 6.7.2 Select the program you want to stain You can select the program you want to stain by pressing the cur- rent program.
  • Page 50 You will be taken to the Program Selection menu. Just click on the short name to choose the pro- gram. Once selected you will be back at the overview screen and the se- lected program is visible. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 51: Start The Program

    6.7.3 Start the program You start the program by pressing the “Start” button. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 52 Sample program – in this case PAP staining: Bath 1 - 3 30 seconds reagent Bath 4 15 seconds water (rinse) Bath 5 3 minutes reagent Bath 6 1 minute water (rinse) Bath 7 20 seconds reagent Bath 8: 30 seconds reagent Bath 9: 1 minute...
  • Page 53 Program with hot air station: Hot air station temperature to cold (blue): Hot air station temperature to warm (red): Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 54 Hot air station error (black): Hot air station set temperature reached (orange): 6.7.4 Symbols during program In mains operation, the battery is showed in grey until it is fully charged. Then the icon disappears. In mains operation, the battery is showed in grey until it is fully charged. Then the icon disappears. In floating operation, the battery icon shows the level, dark blue with sufficient residual charge and red below 25%.

  • Page 55: End Of Program

    6.7.5 End of program After the program is finished, slide baskets are placed in the unload station (capacity 5 slide baskets) and the device indicates by a ton signal. In case the unload station is full, the device gives the following warning message: Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 56: Stop A Running Program

    6.7.6 Stop a running program If you want to stop a running pro- gram, press the “Stop” button.: The following message will appear. Press “OK” and the program will stop. You have to take out the slide baskets manually. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 57: Emergency Stop

    6.7.7 Emergency stop In case of an emergency, the oper- ation function of the Cromatec can be switched off by pressing the emergency stop located at the lower front of the device. To continue the program, release the emergency stop button and confirm the alarm message.

  • Page 58: Errors

    7 ERRORS 7.1 Notes Any failure occurring during the operation of the Cromatec is indicated by an alarm sound and message, shown on the display. Please refer to the trouble shooting list. 7.2 PopUp messages in normal operation After pressing STOP: Red PopUp “Would you really cancel all staining procedures?”...
  • Page 59 Flow of the filter running time: Filter usage time expired, please replace. Yellow PopUp [OK] “INFO“ Expiration of reagent: Yellow PopUp “INFO” At least one reagent expired, please replace. [OK] At start attempt despite emergency stop: Yellow PopUp Resolve problem, unlock “EMERGENCY HALT”...
  • Page 60 In the case of a start experiment with samples still attached to the arm: Yellow PopUp (State after prior emergency stop) “INFO” Specimen found at transport arm. Please remove. [OK] On start with expired filter running time: Yellow PopUp Filter usage time expired please replace. “INFO”...

  • Page 61: Error Messages In Normal Operation

    7.3 Error messages in normal operation Motor Error: Red PopUp Staining procedures interrupted since hh:mm:ss “MOTOR ERROR” [Retry] [Cancel] Messages: Overcurrent horizontal Overcurrent vertical Timeout horizontal Timeout vertical Failure of the magnetic control: Red PopUp Specimen transport impossible “MAGNET ERROR” Manually remove specimen and call service Note: no OK-Button, present program will be cancelled!
  • Page 62 Specimen lost (while lifting down): Specimen lost at n1 n2 n3 (station no.) Correct, then touch OK. Red PopUp [OK] „ERROR“ Specimen holder stick or lost (combination Specimen sticking at n1 n2 n3 (station no.) 8+9): Red PopUp Specimen lost at n4 n5 n6 (station no.) „ERROR“...

  • Page 63: Power Failure

    7.4 Power failure The Cromatec has a built-in battery to allow the unit to work even in a case of power failure. In case of a power failure the display shows the following symbol: The operation is continued using the emergency power supply. As the battery gets loaded during op- eration, a fully loaded device can be operated using the battery.

  • Page 64: Hood

    Red PopUp window is shown. The Cromatec I / II will not perform any staining program while the lid is open. When the lid is opened, the ventilation will automatically be increased. Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 65: Cleaning

    8.1 General cleaning advice The Slee linear stainer Cromatec I / II has a solvent resistant surface area according to the requirements of histological la- boratories so that an easy cleaning can be done without problems. Only the display should be cleaned with a damp cloth.

  • Page 66: Schedule Of Cleaning For Staining System

    8.2 Schedule of cleaning for staining system: _____________________________________________________ Date Staining troughs Water bath Tube System Name Manual_Cromatec_I_II_2024-10-008.00_EN.docx...

  • Page 67: Optimized H&E Program

    8.3 Optimized H&E program Station Reagent Time Xylol 1,30 Isopropanol 1,30 Isopropanol 1,30 96% Ethanol 1,30 96% Ethanol 1,30 Water 1,30 Hemalum 1,30 Hemalum 1,30 Water 1,30 Glacial acetic acid 1,30 Running Water 1,30 Running Water 1,30 96% Ethanol 1,30 96% Ethanol 1,30 Eosin...

  • Page 68: Service

    If the device or parts thereof are sent back in a dirty or non-disinfected condition, SLEE medical GmbH reserves the right to return the parts to the debit of the customer without carrying out repairs or maintenance.
  • Page 69 Notes Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 70 Notes Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 71 Manual_Cromatec_I_II_2024-10-008.00_EN.docx...
  • Page 72 SLEE medical GmbH • Am Neuberg 14 55268 Nieder-Olm • Germany www.slee.de T: +49 (0) 6136 76997-0 E: mail@slee.de www.slee.de YouTube LinkedIn Instagram Manual_Cromatec_I_II_2024-10-008.00_EN...

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