Slee MICROTOME CUT 4062 Operating Manual
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Slee MICROTOME CUT 4062 Operating Manual

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O P E R A T I N G
M A N U A L
M I C R O T O M E
C U T 4 0 6 2
MANUAL
ROTARY MICROTOME
D ES I GN &
M A N UFACT UR I NG
M A DE IN GE R M A NY

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April 25, 2025

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Summary of Contents for Slee MICROTOME CUT 4062

  • Page 1 O P E R A T I N G M A N U A L M I C R O T O M E C U T 4 0 6 2 MANUAL ROTARY MICROTOME D ES I GN & M A N UFACT UR I NG M A DE IN GE R M A NY...

  • Page 3: Table Of Contents

    CONTENTS INTENDED USE _______________________________________________________ 5 SYMBOLS ____________________________________________________________ 5 SAFETY NOTES _______________________________________________________ 5 HAND WHEEL STOP FINGER PROTECTION GUARD WORKING WITH KNIVES AND DISPOSABLE BLADES COMPONENTS ______________________________________________________ 10 MAGNIFIER WITH ILLUMINATION, DIMMABLE (OPTIONAL) SPECIFICATIONS _____________________________________________________ 14 UNPACKING AND INSTALLATION _____________________________________ 15 UNPACKING THE DEVICE INSTALLATION OPERATION OF CUT 4062 ____________________________________________ 16...
  • Page 4 OPTIONAL ACCESSORIES _____________________________________________ 36 WARRANTY _________________________________________________________ 37 DISPOSAL __________________________________________________________ 37 Manual_CUT_4062_2021-10-008.00_EN.docx...

  • Page 5: Intended Use

    1 INTENDED USE The rotary microtome CUT 4062 is intended for cutting thin sections of soft paraffin-embedded and harder specimens for professional use in routine and research laboratories in the fields of biology, medicine and industry. The system of specimen advance operates very reliable from 0.5 to 60 µm. The quality of cutting of this microtome is increased by the automatic retraction during the upstroke of the specimen, which avoids rubbing on the disposable blades or microtome knives.

  • Page 6: Hand Wheel Stop

    MAGNIFIER WITH LIGHTING (OPTIONAL) Risk of glare and injury! Never look into the sun or any other bright light source with optical devices! Do not look directly into the light source. Fire hazard! Lenses in optical devices can cause considerable damage due to the "burning glass effect"...
  • Page 7 3.1.2 HANDWHEEL STOP WITH BRAKE ON THE BASE (OPTIONAL) Always use the finger protection guard with the knife- / blade holder and put hand wheel in stop position • before working with the knife or specimen, • before changing the specimen, •...

  • Page 8: Finger Protection Guard

    3.2 FINGER PROTECTION GUARD Use the finger protection guard always • before you start any work with the knife or spec- Finger protection guard released imen, • before changing the specimen, • during work break. Finger protection guard activated Please note that the finger protection guard may be engaged throughout processing (exchange of blade, lateral displacement, trimming, cutting), thereby increasing operation safety! Do never move your fingers or hands above or behind the blade holder during operation of the microtome.

  • Page 9: Working With Knives And Disposable Blades

    3.3 WORKING WITH KNIVES AND DISPOSABLE BLADES Microtome knives and disposable blades have extremely sharp edges and this can lead to injuries. Please be extremely careful when handling microtome knives and disposable blades. Do not place microtome knives or disposable blades at unsecured areas. Never position micro- tome knives or disposable blades with the sharp edge pointing upwards.

  • Page 10: Components

    4 COMPONENTS Slee rotary microtomes are provided with the following standard components: CUT 4062, #10065000 Specimen orientation • Universal cassette clamp, orientable, alternatively standard specimen clamp, orientable • 3-component knife holder, can be used for low profile blades as well as high profile blades •...

  • Page 11: Magnifier With Illumination, Dimmable (Optional)

    4.1 MAGNIFIER WITH ILLUMINATION, DIMMABLE (OPTIONAL) Mounting: The ring magnifier with illumination can easily be at- tached to the side of the microtome's base plate. 2 M5x12 countersunk screws (supplied) are screwed into the base plate from below. Manual_CUT_4062_2021-10-008.00_EN.docx...
  • Page 12 Switching on / off: The light can be switched on and off via the sensor switch on the light head. Switching on: Touch the sensor for a brief moment -> the light will be switched on (switch-on value is the last dim- ming value).
  • Page 13 Specifications magnifier with illumination: Protection class Protection type IP 20 Operating mode Continuous operation Technical safety check EN 60 598-1 Light head ca. Ø 122 x 13 mm Magnifier 6.0 dpt. approx. Ø 72 Rated voltage 100 - 240 V AC Frequency range 50/60 Hz Power consumption...

  • Page 14: Specifications

    5 SPECIFICATIONS Operating temperature range +10 to +35 °C Operating humidity max. rel. 80 % non-condensing Storage temperature range +5 to +55 °C Storage humidity max. rel. 80 % non-condensing Section thickness range 0.5 - 60 µm Section thickness settings 0.5 - 2 µm in 0.5-µm steps 2 - 10 µm in 1-µm steps 10 - 60 µm in 2-µm steps...

  • Page 15: Unpacking And Installation

    6 UNPACKING AND INSTALLATION 6.1 UNPACKING THE DEVICE Remove the upper wooden cover. Remove the upper supporting foams. Lift the device out of the wooden transportation case. Grasp the device underneath the base plate from the rear and front as shown in the figure below. Do not transport the device by holding it on the hand wheel shaft, the object head, the cover or the tray.

  • Page 16: Operation Of Cut 4062

    7 OPERATION OF CUT 4062 7.1 INSERTION OF SPECIMEN IN STANDARD SPECIMEN CLAMP Turn the handwheel to the highest position and activate the handwheel lock (see chapter 3.1) and the finger guard. Loosen the standard specimen clamp by turning the fixing screw counterclockwise.

  • Page 17: Insertion Of Specimen In Universal Cassette Clamp

    7.2 INSERTION OF SPECIMEN IN UNIVERSAL CASSETTE CLAMP Turn hand wheel to its highest position and activate the hand wheel lock. Activate finger protection guard. Open cassette fixation by pulling the fixation lever. Insert / remove cassette. Object fixation will go back into fixation position automati- cally.

  • Page 18: Specimen Orientation Adjustment

    7.3 SPECIMEN ORIENTATION ADJUSTMENT Turn hand wheel to its highest position and activate the hand wheel stop (see chapter 3.1). For orientation of the specimen, open the fixing lever on the right side of the orientation. For upward and downward orientation use the upper ori- entation wheel (see fig.).

  • Page 19: Exchanging The Clamp Type

    7.4 EXCHANGING THE CLAMP TYPE For exchanging the universal cassette clamp with specimen orientation with the standard specimen clamp (fixed), please proceed as follows: Move the universal cassette clamp to the upper position and fix the hand wheel by pressing the brake lever. Pull out the right handle of the specimen orientation to re- lease the universal cassette clamp out of the system.
  • Page 20 Disassemble the specimen orientation. Disassemble the standard specimen clamp to get access to the center screw – M8 x 50 mm. Install the basic part of the standard specimen clamp and fix it with the Allen screw. Manual_CUT_4062_2021-10-008.00_EN.docx...
  • Page 21 Install the spindle, the washer and the knob. Manual_CUT_4062_2021-10-008.00_EN.docx...
  • Page 22 To replace the universal cassette clamp with specimen orientation with the standard specimen clamp with specimen orien- tation, please proceed as follows: Move the universal cassette clamp to the upper position and fix the hand wheel by pressing the brake lever. Pull out the right handle of the specimen orientation to release the universal cassette clamp out of the system.
  • Page 23 Install the right handle of the specimen orientation to fix the standard clamp. Manual_CUT_4062_2021-10-008.00_EN.docx...

  • Page 24: Insertion And Orientation Of Microtome Knives

    7.5 INSERTION AND ORIENTATION OF MICROTOME KNIVES Turn the handwheel to the highest position and activate the handwheel lock (see chapter 3.1). Remove finger protection guard on the right side of the knife holder. Loosen the knife fixation by turning the two black screws counter clockwise (see fig.).

  • Page 25: Insertion And Orientation Of Disposable Blades

    7.6 INSERTION AND ORIENTATION OF DISPOSABLE BLADES Turn the handwheel to the highest position and activate the handwheel lock (see chapter 3.1). Release the finger guard. Release the blade clamping lever on the left side of the blade holder by turning it counterclockwise. Remove the old blade or insert a new blade (see fig.).
  • Page 26 Horizontal orientation of the blade For adjustment of cutting area of the blade, the blade holder can be moved to the left or right. To do so, release the lever on the lower right side of the swivel piece by turning it counterclockwise (see fig.). Move the knife holder to the left or right.
  • Page 27 Adjusting the sledge clamping 1. Remove the screw with Allen key size 2,5 (pos. 1). 2. Removing washers makes clamping tighter. Add- ing washers loosens clamping. 3. Tighten the screw (pos. 1) and test the clamping by assembling the blade holder. Adjusting the blade clamping 1.
  • Page 28 Modification from low to high blades Before any manipulation of the blade holder remove the blade! The first picture shows the low-profile configuration, un- derneath the low-profile configuration without the blade and the third picture shows the high-profile configuration. To change from low profile to high profile configuration the two screws (pos.
  • Page 29 Changing the lever to the other side - blade clamp Loosen the screw (located on bottom side of lat- eral sledge) with Allen key (see fig., marked with red circle no. 1). Take the lever out of the sledge and put it into the hole from the other side.

  • Page 30: Approach To Specimen

    7.7 APPROACH TO SPECIMEN Turn the handwheel to the highest position and acti- vate the handwheel lock (see chapter 3.1). Loosen the knife or blade holder base by turning the left lever counter clockwise (see fig.). Loosening of knife or blade holder base fixation and move- ment towards specimen Move the blade holder base inside the guiding rail to- wards or away from the specimen (see fig.).

  • Page 31: Coarse Advance

    7.8 COARSE ADVANCE Turn hand wheel on the left-hand side of the device to move the specimen towards the knife or blade. An acoustic signal is emitted at the mechanical end posi- tions. In this case, do not continue to turn in the same di- rection, as this may cause damage to the device.

  • Page 32: Manual Cutting

    7.9 MANUAL CUTTING Release hand wheel stop (see chapter 3.1). For cutting turn the hand wheel on the right side of the device clockwise. A counter clockwise movement of the hand wheel will also result in cutting and the specimen is gradually brought closer to the blade.

  • Page 33: Cleaning And Maintenance

    8 CLEANING AND MAINTENANCE 8.1 CLEANING The recommended frequency of cleaning the microtome depends on how intensely the device is used. Wear protective clothing and disposable gloves according to Good Laboratory Practices. Please take note of the safety aspects of the device. To clean the microtome mild domestic cleaning agents can be used.

  • Page 34: Recommended Maintenance And Service Schedule

    Remove section waste from the waste tray. Please use supplied dust cover when device is not in use. Weekly Cleaning of blade holder. Yearly Annual inspection (performed by authorized Slee service technician) • Check of all functions • Lubricating of movable parts •...

  • Page 35: Service

    If it is necessary to return the device, it must be cleaned and disinfected before delivery. It must be returned in its original packing. If the device or parts thereof are sent back in a dirty or non-disinfected condition, SLEE medical GmbH reserves the right to return the parts to the debit of the customer.
  • Page 36 10 OPTIONAL ACCESSORIES aquatec system #10090056 MTR Bench top quick-freezing unit #10110000 Magnifier with illumination #10090060 Disposable blade holder TC65 (for tungsten carbide blades) #10090041 Tungsten carbide blade (1 pc.) #28406000 Glass knife holder (for triangle glass knives) #10090014 Segment arc incl. universal sample holder (for glass knife holder application) #10090015 Super Mega cassette clamp, orientable #10090027...
  • Page 37 11 WARRANTY SLEE medical GmbH guarantees that the product delivered has been subjected to a comprehensive quality control procedure, and that the product is faultless and complies with all technical specifications and / or agreed characteristics warranted. SLEE medical GmbH guarantees that the device is manufactured under an ISO 9001:2015 and ISO 13485:2016 quality management system.
  • Page 38 Notes Manual_CUT_4062_2021-10-008.00_EN.docx...
  • Page 39 Notes Manual_CUT_4062_2021-10-008.00_EN.docx...
  • Page 40 SLEE medical GmbH • Am Neuberg 14 55268 Nieder-Olm • Germany www.slee.de T: +49 (0) 6136 76997-0 E: mail@slee.de www.slee.de YouTube LinkedIn Instagram INS1000-10GB ∙ Manual_CUT_4062_2021-10-008.00_EN...

This manual is also suitable for:

10065000

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