Flexible staining systems with highest throughput (84 pages)
Summary of Contents for Slee MCS I
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O P E R A T I N G M A N U A L C O V E R S L I P P E R M C S I HIGHLY RELIABLE SYSTEMS FOR A SMOOTH WORKFLOW IN THE LABORATORY HIGHEST QUALITY STANDARDS D ES I GN &...
CONTENTS 1. Safety 1.1 Introduction 1.2 Used symbols 1.3 Intended use 1.4 Emissions 1.5 Sources of danger 1.6 Authorized user / Operator 1.7 Personal protective equipment 1.8 Safety instructions on installation site 1.9 Safety equipment 1.10. Case of emergency 2. Transport / Installation 2.1 Technical data 2.2 Transport / Storage 2.3 Unpacking...
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5. Routine Basics 5.1 Direction of specimen on the slides 5.2 Short introduction 6. Troubleshooting 6.1 Changing the fuses 6.2 Troubleshooting 6.3 Error Messages 7. Abandonment/Recycling 8. Greasing Manual_MCS_I_2021-10-002.00_EN.docx...
1. SAFETY 1.1 INTRODUCTION By purchasing this MCS I coverslipping machine, you decided for a quality product of SLEE medical GmbH, Germany. Intention of this manual is to help you, working with your unit. Please read it carefully and completely and follow its advices.
The work with reagents and medias, as they are used in laboratories to coverslip slides, is always a danger for health and life. So also by using the MCS I be sure to follow the common rules and regulations for work with these medias. Further danger can be caused by the moving mechanical parts (step motor axes) inside the MCS I.
In this case a restart is offered by the MCS I. For some reason it can be necessary to have the unit stopped immediately. In this case you have to push the “ON/OFF”...
Storage humidity max. rel. 80 % non-condensing 2.2 TRANSPORT / STORAGE The coverslipping machine MCS I is delivered in a protection box. Please move it only upright and avoid shocks. 2.3 UNPACKING Put the machine on a flat and stable underground and remove the packing. You should keep the material for safety in cases of later transports, if possible.
The unit is delivered “ready to start”. For the connection to your grounded laboratory power supply use the delivered cable. The MCS I can be used with a charcoal filter and has two powerful exhaust fans and so it can be used even in laboratories without separate central exhaust-system. The filters have to be exchanged at least once a year, depending on frequency of use.
(reservoirs), which easily show the fill level all the time. To refill the cartridges, you may open the MCS I, after it took in its refill position and opens the hood after switching on, or anytime during operation, by pressing the “BREAK” button).
Also check for the cover glass waste container and the purge cap (mounting media waste, which has to be replaced and emptied regularly). The purge caps are available as consumables at Slee. The MCS I asks you to confirm your check by pressing “OK”. Manual_MCS_I_2021-10-002.00_EN.docx...
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Refill, clean, take out Refill, clean, take out The needle cartridge on the left (preventing of needle dry out) is always free for taking out, while check function at start-up and when hood is released anytime you use the “BREAK” function. It can be taken out by pulling it up.
You will have to exchange / empty the purge cap after the MCS I asks you to check xylene and Pertex® 3 times during this cycle. You should restart the “Cleaning“ run perhaps for a second and third time after it has finished.
Open a bottle of Pertex® and put it into the designated position at the left inside wall of the MCS I. The end of the tube has to be placed into the bottle. With a special adaptor it can be secured against falling out as shown on the left picture.
The “delay” time should only be changed, if you have problems with the start point of the line.(~150ms). For first tests Slee recommends to set the volume to the lowest value (30µl) and to increase this volume during the test runs till results are optimized. This can be done easily during the process with „volume+”...
0,19mm to receive best results without having problems of sticking to each other, that can lead to crashes and damage of slides in bad cases, as the MCS I is working very fast and has to bring all slides back into the basket, which may be impossible if too many covers or splinters are placed there.
CG-box. To refill the box, go on like shown in the next pictures. If the MCS I still works, just stop a running process with the „BREAK“ key, to release the cover and hold all movements.
“SETUP” to get into the SETUP menu. Then press “Change CG”. The MCS I will go to a special position and release the hood to have best possibility to adjust the coverglass container to the CG-Head.
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The CG-Head can also be adjusted by just turning the adjustment-wheel (in the upper area) as seen in the left picture. So here again is the information, to check with your fingers, if space to get into the coverglass box in front and on the backside is equal so it should be perfect for the CG-Head to get inside the box.
For best variability in processing and to be also able to run low-grade coverglass, Slee offers the MCS I with 2 versions of CG-Head. A version with 2 vacuum pads for best coverslipping results and fastest processing - as standard. Another version with 1 vacuum pad and one separator for best separation in cases of substandard quality coverglass or high humidity.
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Whatever CG-Head system you use, it is absolutely necessary to keep the vacuum pads absolutely clean from any dirt or mounting media. For cleaning, you may pull the pads off to clean them with xylene or suitable solvent or to replace them. Only put them back when they are dry, otherwise the pads will stick to your coverglass when starting the next process.
The MCS I checks for free storage positions regularly. So if it detects 4 full stations, it will inform the user by a message in the display and a small alarm, to be sure, somebody will empty the positions.
4. REGULAR CLEANING AND MAINTENANCE 4.1 CLEANING INSTRUCTIONS The Slee MCS I is a precision instrument. So it is necessary to take care and to keep the device free from broken glass or stuck mounting media. Specially the turntable and its guiding’s and position rollers should be cleaned carefully and regularly with a brush and...
Attention: Before doing any cleaning work, the power cable has to be disconnected first! A regularly maintenance of the MCS I (cleaning and greasing of the relevant mechanical parts, cleaning of parts inside and exchange of expandable parts) by SLEE medical GmbH service is the main requirement for a well working machine! Ask your local SLEE medical GmbH representative for a maintenance check.
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(MAS). It is always the front side, which is being coverslipped. So, already by starting the staining programs, you have to insert the baskets with specimen into your direction! When using the MCS I as stand-alone unit, you will have to do it the same way - put the baskets with specimen towards you into the loading station.
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MAS or by using a separate available manual loading station. There is no further switch to be pressed to start, just a basket has to be detected by the MCS I 8. Information like “cover glass” level in % or “volume” of mounting media in µl is shown on the screen and the „Counter“...
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11. All the time during any process, you have the possibility to stop the MCS I with “BREAK”, to perhaps replace cover glass or check for mounting media. The unit then stops in a safe position after coverslipping the last slide and releases the hood locking. Now it is possible to open the cover and for example refill coverglass or re-empty the waste containers.
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TROUBLESHOOTING 6.1 CHANGING THE FUSES In case of malfunction, you should check first that your power supply is OK and be sure the main switch is turned “ON”. Check the fuses inside the fuse box next to the power connector at the back panel. For this, proceed as follows: Disconnect power cable, open the fuse box carefully with a screw driver, check the fuses and replace them, if necessary.
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The first number shows the step motor-number, which created the error code. The second number (mostly 1 or 2) shows more detailed information for Slee service people. 1. stands for „reference error“ (sensor not reached due to blockage, broken motor, broken sensor, cable or bad connection) 2.
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(slideglass). All these errors normally lead to switch of the unit, to check the axes for blockage by the user. If this doesn't lead to success, SLEE medical GmbH trained Service engineers should check for reasons. After a switch-off you will have to take out the remaining slides and the basket by hand before you switch on again.
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7. ABANDONMENT / RECYCLING After expiration of instrument’s life span please transfer unit into professionally disposal or send it back to SLEE medical GmbH. As a manufacturer / distributor we take back the used equipment, requiring a payment for disposal.
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