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Related Manuals for Astrea Evolve
Summary of Contents for Astrea Evolve
- Page 1 Technical User Guide Evolve ™ Process Columns (200 & 250mm diameter columns)
- Page 2 PC: Evolve 200_250 Columns Operating Manual v2...
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Page 3: Table Of Contents
(1) Installation of bed supports ..................12 (2) Installation of Fixed Bed Support ................12 (3) Installation of Height Adjustable Feet ................. 13 (4) Levelling the Evolve™ Process Columns ..............13 (5) Installation of Adjuster Bed Support ................14 (6) Setting the Bed Height ....................15 4. - Page 4 Reassembly of Evolve™ Process Columns ................24 Fixed Assembly ......................... 24 Adjuster Assembly ......................25 6. Troubleshooting ........................28 Leakage from Adjuster Seal....................28 Leakage from Fixed Bed Support Seal ................. 28 Leakage from Inlet or Outlet ....................28 Excessive Column Back Pressure ..................28 Media Leakage ........................
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Page 5: Table Of Figures
Table of Figures Figure 1 Pressure Drop Evolve™ 200 mm Process Column Figure 2 Pressure Drop Evolve™ 250 mm Process Column Figure 3 Diagram showing the location of the fixed bed support Figure 4 Photograph showing the installation of the fixed bed support... -
Page 6: Column Hardware
Prevents any product inactivation through metal contact. 2. Unlike other columns available on the market the Evolve™ Process Columns have an integral bed support and seal assembly. The polypropylene mesh and seal are manufactured as a single unit and fits directly on the flow cell without any fixings, which has the following advantages;... -
Page 7: Technical Data
Maximum diameter including casters (mm) Maximum height (mm) 1550 1550 Minimum clearance** (mm) 1750 1750 *based on bed height of 520 mm and specific gravity 1.05 **minimum height required to remove adjuster from acrylic tube unit PC: Evolve 200_250 Columns Operating Manual v2... -
Page 8: Pressure Drop
The tests were performed at ambient temperature 15-20°C using RO water. Figure 1 Pressure Drop Evolve™ 200 mm Process Column Figure 2 Pressure Drop Evolve™ 250 mm Process Column PC: Evolve 200_250 Columns Operating Manual v2... -
Page 9: Material Conformity
Lifting eye (200 mm and 250 mm only) Stainless steel 316 Support Leg Stainless steel 316 Foot (200 mm and 250mm only) Stainless steel with rubber Bolts, screws, washers Stainless steel type 316 PC: Evolve 200_250 Columns Operating Manual v2... -
Page 10: Chemical Compatibility
Dimethyl formamide Dimethyl sulphoxide 10% Disodium phosphate Ethanol 20% Ethanol 70% Ethylene glycol (1,2-ethanediol) Formaldehyde 50% Glycerol (Glycerine) PC: Evolve 200_250 Columns Operating Manual v2... - Page 11 Sodium hypochlorite 200 ppm Sodium nitrate Sodium sulphate Trichloroethene (Trichloroethylene) Triton™ X-100 surfactant Urea 6.0 M Zinc chloride PC: Evolve 200_250 Columns Operating Manual v2...
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Page 12: Safety Guidelines
• The product pressure x volume is equal to or less than 200 bar litres. Over-Pressurization Users of the Evolve™ Process Columns must ensure that the maximum operating pressure is not exceeded. It is recommended that an over-pressurization protection device such as a ‘Pressure Relief Valve’... -
Page 13: Column Installation And Operation
Leveling the column Setting the bed height (1) Installation of bed supports The Evolve™ Process Column features polypropylene, woven mesh bed supports, integrated with a Santoprene ® seal. These do not require pre-wetting before use and are required to be fitted directly onto the adjuster and fixed flow cell. -
Page 14: Installation Of Height Adjustable Feet
3. Locate fixed flow cell into the tube unit and fix in place. (3) Installation of Height Adjustable Feet The 200 mm and 250 mm Evolve™ Process Columns are supplied with transportation castors. These assists with the removal of the columns from their packaging and enable the column to be located in the process area. -
Page 15: Installation Of Adjuster Bed Support
See Figure 6 below. Figure 6 Diagram showing the location of the adjuster bed support 3. Work around the circumference of the flow cell ensuring that seal is correctly fitted. See Figure PC: Evolve 200_250 Columns Operating Manual v2... -
Page 16: Setting The Bed Height
3. Partially energize the seal and turn the height adjust handles clockwise to set the required bed height. 4. Check the column is level by placing the spirit level on the adjuster flange. PC: Evolve 200_250 Columns Operating Manual v2... -
Page 17: Column Operation
Hydrostatic Leak Test The purpose of this test is to check that there are no leaks from the Evolve™ Process Column and the column is ready to use. Due to the materials of fabrication, when the column is initially exposed to pressure the column will flex. -
Page 18: Column Packing (Constant Flow Rate Method)
18. To lower the adjuster, gently release some of the seal compression, and turn the height adjust handles clockwise, buffer will eject from the flexible tube. Continue until the bed support touches the top of the packed bed. PC: Evolve 200_250 Columns Operating Manual v2... - Page 19 NOTE: It is advised to provide additional support if large valves or stainless-steel pipework is connected to the top adaptor. CAUTION: Do not adjust the seal actuation knob when the column is under pressure. PC: Evolve 200_250 Columns Operating Manual v2...
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Page 20: Figure 8 Schematic Of The Column With Part Identification
Figure 8 Schematic of the column with part identification PC: Evolve 200_250 Columns Operating Manual v2... -
Page 21: Unpacking
0.1 - 0.5 M sodium hydroxide or 0.1 M sodium hydroxide + 20% ethanol, both of which are suitable for long term (several months) storage with Evolve™ Process Columns. Unpacked columns should be kept in a cool, dry, dust-free location and protected from light. In particular, do not expose acrylic column tubes to direct sunlight for prolonged periods or sterilize with ultra-violet lights as this may reduce the mechanical strength of the tube. -
Page 22: Maintenance
Routine Maintenance Procedures and General Guidelines To disassemble and reassemble the Evolve™ Process Columns follow the instructions below. Note: after reassembling the Evolve™ Process Column ensure that the column does not leak by performing a hydrostatic leakage test. When assembling/dissembling attention should be paid to: CAUTION! Column units are heavy and can roll 1. -
Page 23: Disassembly Of Evolve™ Process Columns
- a small extraction tool is provided. Note: care must be taken not to damage to seal or the flow cell. Figure 9 Adjuster Assembly for EvolveTM 100 Process Column PC: Evolve 200_250 Columns Operating Manual v2... -
Page 24: Fixed Assembly
4. To remove the mesh/seal assembly from the flow cell carefully prize the bed support seal from the flow cell. Figure 10 Fixed Assembly for Evolve 250 Process Column Figure 11 Position of the support blocks before the fixing bolts are completely removed PC: Evolve 200_250 Columns Operating Manual v2... -
Page 25: Tube Assembly
3. Fit all the process pipework and ensure all connections are secure but do not overtighten. Figure 12 Diagram showing the location of the fixed bed support Figure 13 Photograph showing the installation of the fixed bed support PC: Evolve 200_250 Columns Operating Manual v2... -
Page 26: Adjuster Assembly
Ensure the seal is pushed tight into the groove. The bed support should be fitted with the smooth side facing into the column, rougher side to flow cell. See figure 16 below. Figure 15 Diagram showing the location of the adjuster bed support PC: Evolve 200_250 Columns Operating Manual v2... -
Page 27: Figure 16 Photograph Showing The Final Location Of The Adjuster Bed Support
CAUTION - Do not over tighten as over tightening this adapter can lead to failure of the component Figure 17 Photograph showing the alignment of markings on flow tube and sanitary clamp adapter to ensure component is correctly tightened. PC: Evolve 200_250 Columns Operating Manual v2... - Page 28 Lift the adjuster unit into the acrylic column tube. Note: alignment pins are located in the top flange to assist orientation. CAUTION - Adjuster units are heavy 10.Locate the flange fixing bolts in the underside of the column tube flange and tighten. PC: Evolve 200_250 Columns Operating Manual v2...
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Page 29: Troubleshooting
(e.g. partial valve failure), the media packed bed (e.g. over compression or bed fouling), the process fluid, or problems with the column. Only issues relating to the column are dealt with in this section: PC: Evolve 200_250 Columns Operating Manual v2... -
Page 30: Media Leakage
Damage to the adjuster seal or misalignment of the seal causing failure of the seal setting mechanism. Check that the particle size of media is suitable for the pore size of bed support. Remove all media from the column and inspect the column. Replace components as required. PC: Evolve 200_250 Columns Operating Manual v2... -
Page 31: Ready To Use
7. Ready to Use Kits supplied with Column The following 3 x kits are supplied with the Evolve™ Process Column. Note: to purchase any replacement parts please see our ‘Spare Parts, Kits & Accessories’ section (p31 onwards). Tool kit PRODUCT NUMBER... -
Page 32: Spares Kit
Bed support assembly, 200 Adjuster, Bed support assembly, 250 Fixed, Bed support assembly, 250 Adjuster, Adjuster seal, Evolve™ 200 mm Adjuster seal, Evolve™ 250 mm Flow tube seal,7.0 mm Bore Flow tube seal, Ladish 7.0 mm Bore PC: Evolve 200_250 Columns Operating Manual v2... -
Page 33: Spare Parts, Kits & Accessories
8. Spare Parts, Kits & Accessories Please do not hesitate to contact us via sales@astrea-bio.com if you would like to re-order any of the components described in this section. Column Refresh Kit PRODUCT NUMBER DESCRIPTION Sanitary Clamp Adapter 6 mm Bore... -
Page 34: Wetted Flow Path Kit
Bed Support Assembly, 250 Adjuster Anti-jet Disc Large Bed Support Kit PRODUCT NUMBER DESCRIPTION Bed Support Assembly, 200 Adjuster Bed Support Assembly, 200 Fixed Bed Support Assembly, 250 Adjuster Bed Support Assembly, 250 Fixed PC: Evolve 200_250 Columns Operating Manual v2... -
Page 35: Elastomer Seal Kit
Acrylic Tube, 200 X 530 mm Acrylic Tube, 250 X 530 mm Buffer Ring, 200 mm Buffer Ring, 250 mm Scale Label, 200 mm Scale Label, 250 mm Serial Number Label Rating Plate Evolve™ label Fitting Instructions PC: Evolve 200_250 Columns Operating Manual v2... -
Page 36: Torque Wrench Kit
Flexible Hose 6 mm Diameter x 2.0 m AAFHK2 Flow Tube Seal, Ladish 7.0 mm Bore Click Clamp, 3/4" Ladish Castor/ Tow Kit DESCRIPTION Product Number Spanner 22mm/ 24mm AACTK Caster Assembly, Braked Fitting Instructions PC: Evolve 200_250 Columns Operating Manual v2... -
Page 37: Adjustable Foot Kit
Adjustable Foot Kit DESCRIPTION Product Number Adjustable Foot AAFK Spanner 22mm/ 24mm open ended Fitting Instructions PC: Evolve 200_250 Columns Operating Manual v2... -
Page 38: Chromatography Performance
Figure 18 Description of Column Efficiency Calculations Asymmetry is measured at 10% of the peak height and is calculated per the equation shown below, where a and b relate to the width of the p section. PC: Evolve 200_250 Columns Operating Manual v2... - Page 39 Caution must be taken when interpreting performance results. It is advised to check not only the media type used but also the flow rate, column diameter and bed height. Often results are given for tests performed in laboratory scale columns. PC: Evolve 200_250 Columns Operating Manual v2...
- Page 40 Evolve™ is a trademark of Delta Precision Ltd. HydroPure™ is a trademark of Contec. Santoprene™ is a trademark of ExxonMobil. PuraBead ® - this product or portions thereof is manufactured and sold under license from GE Healthcare under US patents 6841097 and 7341202 and any corresponding patents for life sciences research and the purification/separation of naturally occurring or recombinant plasma derived proteins.
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