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Guide to Replacing the Wetted Flow path 200mm and 250mm Chromatography Column...
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DELTA PRECISION LTD Chromatography Column Guide to Replacing the Wetted Flow path 200mm and 250mm Columns...
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Revision History Revision Comments Date Initial release 9/11/2014 Cover photograph added 26/2/2015 Address updated 21/06/2018 Updated to reflect Evolve® Trademark status 12/07/2021 Manual Revision Date July 2021 Evolve® is a registered trademark of Delta Precision Ltd. Evolve® is a registered trademark in the EU, UK and US.
T able of Contents Replacing the Wetted Flowpath ..................4 Kit components ............................5 General guidelines ......................6 Replacing the wetted flowpath components ....................7 Disassembly of Adjuster Assembly ......................7 Disassembly of the Fixed Assembly ......................8 Disassembly of Tube Assembly ......................10 Reassembly of 200mm and 250 column.
Replacing the Wetted Flowpath This manual describes the procedures necessary to replace the wetted flowpath components. Figure 1.1 Column schematic. Parts shown in are the wetted flowpath components.
Kit components The kit consists of the following components. Please refer to the original column manual for the component materials and chemical compatibility. PRODUCT NUMBER DESCRIPTION COLUMN Flow Tube Adaptor 6mm Bore RENEWAL Flow Tube 6mm Bore Flow Tube Seal,7.0mm Bore Bottom Flowtube Adjuster Seal ,200mm Column Adjuster Seal ,250mm Column...
General guidelines To disassemble and assemble the columns follow the instructions below. After reassembling the column ensure that the column does not leak by performing a hydrostatic leakage test. When assembling/dissembling attention should be paid to: Always use the correct size tools. A comprehensive tool kit was provided with the original column.
Replacing the wetted flowpath components There are three stages to replacing the wetted flowpath. 1. Disassemble the column into the three main sub assemblies 2. Exchange the components 3. Reassembly the column and perform initial integrity checks. Disassembly of Adjuster Assembly 1.
Fixed End Support Block Figure 1.4 Photograph showing position of the support blocks before the fixing bolts are completely removed. 4. Using the fixed end fixing tool and torque wrench, loosen each of the fixing bolts. Remove the flowcell and place it on a clean work surface. 5.
Disassembly of Tube Assembly Figure 1.5 Tube Assembly Evolve® 200 column 1. Using the correct Allen key undo the Tube support Rod bolts located in the top column flange and remove the top column flange. This exposes the tube. 2. Carefully remove the tube and the buffer seals. TUBE ASSEMBLY Wetted Flowpath components Discarded Non-wetted components RETAINED...
Reassembly of 200mm and 250 column. Reassembly of the column is the reverse of disassembly. Tube Assembly 1. The orientation of the tube is shown by the fitted scale label. 2. The buffer seals should be place on the outside diameter of the tube at a distance approximately 2cm from the end of the tube.
Figure 1.8 Photograph showing the installation of the fixed bed support 6. Ensure the support blocks are turned outwards to allow the flowcell to be located. See Figure 1.9 below. Figure 1.9 Photograph showing position of fixed end support blocks to enable relocation of the flowcell.
Adjuster Assembly 1. Install to antijet in the flowcell by carefully locating the 3 pins into the ribbed face of the flowcell. 2. Install the adjuster mesh by carefully locating the bed support seal into its location groove in the side of the adjuster cell. Ensure the seal is pushed tight into the groove. The bed support should be fitted with the smooth side facing into the column.
6. Slide this assembly into the threaded height adjust rod/ seal setting ring assembly and ensure the adjuster seal fits over the flowcell. See Figure 1.11 below Adjuster Seal Seal Figure 1.12 Photograph showing the final location of the adjuster bed support 7.
Preparing for Column Use After replacing the wetted flow path it is recommended before use these instruction are followed. Hydrostatic Leak Test The purpose of this test is to check that there are no leaks from the column and the column is ready to use.
Contact Us To view our Evolve® Column User guides please visit: www.astreabioseparations.com/resources/evolve -column-user-guides For further enquires please contact: sales@astrea-bio.com...
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