Analyticon Hemolyzer 5 Service Manual
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Analyticon Hemolyzer 5 Service Manual

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Summary of Contents for Analyticon Hemolyzer 5

  • Page 1 Servicemanual Servicemanual...
  • Page 3 Despite careful revision and multiple grammar and content control, mistakes can still be present in this manual. Analyticon may from time to time issue errata, or a new revision of present in this manual. Analyticon may from time to time issue errata, or a new revision of the manual.

  • Page 4: Table Of Contents

    Servicemanual Servicemanual Table of ble of Contents Contents TAB L L E OF E OF CONTENTS CONTENTS ................................................3 ..3 INTRODUCTION ..................INTRODUCTION ..................................8 8  .............. .......................... 8 8 HO SHOULD READ THIS MANUAL HO SHOULD READ THIS MANUAL FOR YOUR FOR YOUR SAFET...
  • Page 5 6.9.2 Short guide for laser values ....................71 VERIFICATION PROCEDURES ....................74 ..........................74 TEST 7.1.1 Hemolyzer 5 Self-Test Guide .................... 75 7.1.1.1 General information ........................ 75 7.1.1.2 Electronic Self-Test parameters ....................75 7.1.1.3 Pneumatic Self-Test parameters ..................... 77  ........................
  • Page 6 8.2.3 Gather Your Peripherals Devices ..................106 ..................... 106 ERFORMING THE NSTALLATION 8.3.1 Move the ‘Hemolyzer 5’ to the Selected Location ............106 8.3.2 Visual Inspection......................106 8.3.3 Remove the Protective Card from t he Shear Valve ............106 8.3.4 Connect the Optional Auto-Sampler ................
  • Page 7 Hemolyzer 5 8.3.7 Verify the ‘Hemolyzer 5’ Computer Operation ..............109 8.3.8 Connect Peripherals ......................109 8.3.9 How to install a printer ..................... 110 8.3.10 Initializing the Optional Autosampler ................110 8.3.11 Using the Settings Menu ..................... 112 8.3.12 Set Up a Laboratory Information System (LIS) ............112 8.3.13...
  • Page 8 Servicemanual 10.1.2.1 Shear Valve cleaning ......................130 10.1.2.2 Cleaning the wash head ....................... 130 10.2 ....................130 REVENTIVE  AINTENANCE 10.3 ......................136 LOW CELL CLEANING REMOVAL AND REPL ACEMENT PROCEDURES ..............139 11.1 ..................... 139 PENING THE INSTRUMENT 11.2  A  ...

  • Page 9: Introduction

    This Service manual is intended for trained technicians or service engineers to be able to maintain and repair Hemolyzer 5 automated hematology analyzer. It contains the functional descriptions of the analyzer, operation of the f luidic system, adjustments and settings, and very important information for the Service Personnel about the service operations and possible problems.

  • Page 10: For Your For Your Safet Safety

    To prepare the analyzer for shipping, storage or extended periods of inactivity, please drain the reagents and repackage the Hemolyzer 5 in its original packaging. Do not expose the Hemolyzer 5 to direct sunlight, extreme temperature or humidity (>80%).

  • Page 11: Actors

    Genuine reagents and service materials and spare parts are available from Analyticon. Only Analyticon certified service personnel that have successfully completed the ‘Hemolyzer 5 Service Training’ program are qualified to service the Hemolyzer 5 analyzer. Before operating Hemolyzer 5 analyzer, all operators should complete an ‘Hemolyzer 5 Operator Training’...

  • Page 12: Equirements

    Use only the power cord supplied with the instrument. Avoid using extension cords. Hemolyzer 5 comes with a power cord appropriate for your power system. Proper use of the appropriate power cord assures adequate grounding of the system. If the power is not reliable, contact your representative for options such as the installation of an external UPS module.

  • Page 13: Emolyzer 5

    Hemolyzer 5 Analyticon does not supply UPS for Hemolyzer 5. Therefore, Analyticon is not taking responsibility or liability for the operation of Hemolyzer 5 running on UPS. If you need further information, please, contact technical support of the UPS vendor.

  • Page 14: Isposal

    If Hemolyzer 5 needs to be powered off due to an emergency situation (like fire, thunderstorm, etc.), follow the procedures in User Manual section 7.3.4. In case of fire, do not use water to extinguish the fire unless Hemolyzer 5 is disconnected from the electrical network!

  • Page 15: Structure Of The Analyzer

    Display scr een and the touc h sensiti ve sur face Hemolyzer 5 uses a color LCD with LED backlight touchscreen as main user interface. The built-in touchscreen is a 4 wire resistive type. It is interfaced to the mainboard by a USB...

  • Page 16: Start Button Start Button And Le And Leds Ds

    Shear Valve assembly consists of the following parts: Shear Valve holding plate, • Moving mechanism, • Stepper motor, • Ceramic Shear Valve, • Shear Valve optoboard. • 3.2.2 Sample rotor Hemolyzer 5 hematology analyzer has a sample rotor for safety and more precise sample handling.

  • Page 17: Main Dilutors

    Tube adapter 3.2.3 Main Dilu tors Hemolyzer 5 has two separate main dilutor modules. There are two stepper motors, a common motor opto board, four syringes and piston rods with gear transmission in each module. The software identifies and moves four dilutors (Dil1, Dil2, Dil3, Dil4), each dilutor consists of two syringes.

  • Page 18: Dilutor Opto Se Dilutor Opto Se Nsor Boards

    Servicemanual 3.2.4 Diluto r opto sensor boards Dilutors have their own separated opto-boards, located directly in the units. On the front side of the optoboards there are 4 optosensors and 4 control LED-s, on the back side are mounted the motor connectors and the flat cable connector.

  • Page 19: Laser Head

    Hemolyzer 5 temperature. It contains a massive, molded aluminum block. There are multiple, curved and interconnected stainless steel tubes (fluid paths) inside the TCU to ensure proper capacity. Parts of TCU: in-line mixer, for mixing and homogenizing sample and specific reagents.

  • Page 20: Sample Injector

    Servicemanual 3.2.8 Laser Head Assembly + Sample Inject or Laser Head Assembly is responsible for detecting the 4diff and BASO cells from the prepared blood sample. The laser head is responsible for the precise illumination of the sample. The temperature controlled laser diode source is mounted on a huge brass basis which holds it tight and also responsible for the cooling.

  • Page 21: Laserdiode Laserdiode Driver Driver Board Board

    Hemolyzer 5 3.2.9 Laserdiod e Driver Board The LASERDRV board is based on a PIC 24FJ64GA004 (Microchip) microcontroller that performs all the control functions of the laser diode and the communication with the LS-DACQ (DimmBoard). The LASERDVR board incorporates a laser diode with built-in  APC (automatic power control).

  • Page 22: Eft Side

    Servicemanual 3.3 Left side 3.3.1 Valve boar ds The Hemolyzer 5 system incorporates 44 valves. The valve boards are controlled by two Pneumatic and Power Board (PPB) boards. The used valve boards are the following: 2x Valve_1-5 2x Valve_6-12 2x Valve_13-18 2x Valve_19-22 4 valves are not used, and thus not installed.

  • Page 23: Wbc/Baso

    Hemolyzer 5 3.3.2 WBC/BASO Preheater Assembl y WBC/BASO preheater assembly The WBC/Baso preheater assembly is located on the right side of the impedance measurement block, near the WBC/BASO counting chamber. It consists of two stainless steel holding plates, thermal insulation, heater block (see illustration below) and electronic board with heating transistors.

  • Page 24: Head

    Servicemanual Both chambers have a reference electrode and a draining outlet. The next picture shows the chambers and the measuring tubes. The aperture is made of ruby and it is molded into the measuring tube. Reference electrode chamber chamber Reference electrode WBC measuring tube RBC measuring tube...

  • Page 25: Cell Coun Cell Counter Ter Amplifier Amplifier Board Board

     / CNT ≅ diluent light sample light Due to backlight correction, Hemolyzer 5 is less sensitive to incident light changes. 3.3.5 Cell coun ter Ampli fier Board  Amplifier board includes its own voltage regulators, connection interfaces to HGB head, to high voltage board and to LSDACQ board.

  • Page 26: Pressure Sensor Boar Pressure Sensor Board

    Servicemanual Connection to the electrodes Offset potentiometer Connection to HGB head  Amplifier board includes one input connector for each measuring chamber (measuring electrode). There is one opto switch (OPT1) and a relay (REL1) to connect high voltage to one of the probes with HSW signal and to isolate the input of the amplifier. Test circuit allows generating test pulses (with TEST and PLS signals through Q1, Q2 FETs) for checking proper operation of each amplifier channel.

  • Page 27: Reagent And Reagent And Vacuum Vacuum Buffers Buffers

    Upon replacing the pressure meter board, it is recommended to adjust pr essure meter offset Hemolyzer 5 is equipped with sensitive pressure meters. These pressure meters are used to monitor and control measurement, and chamber draining processes. In some cases, the pressure meters can develop an offset value that can cause “Timeout”...

  • Page 28: Board

    Servicemanual process (for moving the solution). This vacuum is always adjusted according to measured atmospheric pressure. The four next chambers are used as temporary storage volumes for individual reagents for one measurement cycle. The first two (in the front, marked as ‘5-6’ are linked in parallel to double the capacity.

  • Page 29: Pneumatic And Power Pneumatic And Power Boards (Pp Boards (Ppb1 And Ppb B1 And Ppb 2) 2)

    Hemolyzer 5 3.3.11 Pneumati c and Power Boards (PPB1 and PPB2)  card contains the main power regulator circuits, valve and motor driver circuits and other connections for the fluidic and pneumatic system’s parts. Power system  generates +5V (Digital power), +8V (Printer power) and +12V (Motor and valve power) from the single +12V DC input signal.

  • Page 30: Pump Pump Assembly

    Servicemanual blinking: communication in progress - normal state • on (just lighting): OK - normal state • PPB boards have a small board on the bottom called PPB CON board. This board connects the PPB to the LSDACQ board. 3.3.12 Pump assembly Pump assembly generates regulated vacuum and drains the fluidic system.

  • Page 31: Ight Side

    Hemolyzer 5 Hemolyzer 5 3.4 Right Right si side 3.4.1 3.4.1 XY un unit it This unit contains slides to move the sample sampling This unit contains slides to move the sample sampling needle in Horizontal and Vertical directions, two stepper...

  • Page 32: Xyropto Board

    Servicemanual Servicemanual 3.4.2 .2 XYROpto ROpto Boar Board d Horizontal and Vertical motors and the Sample Rotor unit have a common Opto-board. Horizontal and Vertical motors and the Sample Rotor unit have a common Opto-board. Sample Sample Motor Motor Rotor opto Rotor opto connections connections...

  • Page 33: Wash Head Wash Head

    Hemolyzer 5 Hemolyzer 5 3.4.5 3.4.5 Wash Wash head head Wash head is located at the bottom of the Wash head is located at the bottom of the XY unit and it is for XY unit and it is for cleaning cleaning the outer surface of the sampling needle.
  • Page 34 Servicemanual handles all the measuring processes. The Low level SW can be updated from the service software update menu. Flash (BIOS) Vortex86DX Edge MicroSD Card Flash (BIOS) FPGA interface The FPGA is connected through the ISA bus of the DimmBoard. The FPGA implements several registers, and the DimmBoard can reach these registers as memory-mapped.
  • Page 35  As the FPGA is SRAM based, it is configured after every power on. The program is stored in a Xilinx configuration flash memory. The flash memory can be programmed through a JTAG port by a Xilinx Parallel Cable or in-circuit from the Hemolyzer 5 program.  Analo g i np ut s The LS-DACQ has 4 analog inputs (AIN0-AIN3).
  • Page 36 Servicemanual Power voltages on the LS-DACQ board: +12V – Provides +12V to the Cell Counter Amplifier board (AJ5-MEAS), Pin Photodiode and  Amplifier board (OPTSENSOR), High Voltage Board (AJ-HVB) and the Laser Driver board (LASERDRV). -12V – Generated by a DC-DC converter. Not used on the LS-DACQ, output to the Cell Counter Amplifier board (AJ5-MEAS) and the Pin Photodiode and Amplifier board (OPTSENSOR).
  • Page 37 Hemolyzer 5 The LS-DACQ board provides power and an I C interface to the LASERDRV board through the LASER DRIVER connector. PRESSURE Connector The LS-DACQ board provides power and an I C interface to the PRESSMEAS board through the PRESSURE connector.

  • Page 38: Mini-Itx Pc Mot Her Board (In Mini-Itx Pc Mot Her Board (In Hardware Block)

    Servicemanual 3.4.7 Mini -ITX PC mot her board (In Hardware blo ck) 1. PS/2 mous e port (green).  This port is for a PS/2 mouse.  This 9-pin COM1 port is for serial devices. 2. Serial c onnector. 3. DVI connector.  This 24-pin DVI port connects to a DVI monitor.

  • Page 39: Electronic Block Diagram

    Hemolyzer 5 4 Electronic block diagram The analyzer hardware consists of two systems, an Analytical Unit and a Data Acquisition Unit. The two autonomous systems communicate via an USB interface. A 400W ATX PC power supply generates the necessary voltages for the two units.
  • Page 40 Servicemanual 10.4” LCD- LED 600x800 Touch screen Data and Power connector Back light connector connector Touch screen USB Power supply interface board Power & Video Power supply connector Mini-ITX main board PS2 Mouse 4 USB  Audio Keyboard  Audio Keyboard Mouse 3.5”...

  • Page 41: Nit

    Hemolyzer 5 4.2 Data Acqui sit ion Unit This unit executes the commands of the Analytical Unit. It controls all the processes of the sampling, sample actuation, motor and valve control, measurement control and the data acquisition. It preprocesses the raw sampled data and forwards it to the analytical unit.
  • Page 42 Servicemanual   r    t   o   n   s   e   n   s   s   s   s   s   s   e   e    l   e   e   s   e   e   e   e   e   m    l   v    l   v   e    l   v    l   v    l   v    l   v    l   v    S   s   n...

  • Page 43: Mplifier Board

    Hemolyzer 5 4.3 Ampl ifi er board This unit is responsible to gather information during impedance measurements. The amplifier board receives the signal of cells gathered by the aperture and gains these analog signals before transferring it to the LSDACQ.

  • Page 44: Optical Head

    Servicemanual 4.4 Optic al Head Optical head provide WBC differential result applying a hydrodynamic focused flow cell, a laser light, light detectors and amplifiers to gain the detector signals. The laser driver unit has two Safety switches on the Laser head cover fixing to turn off the laser light when the cover is removed.

  • Page 45: Operation Of The Fluidic System

    Hemolyzer 5 5 OPERATION OF THE FLUIDIC SYSTEM This section describes the main fluidic steps of Hemol yzer 5 measurement cycle. The following figures show total measurement flow diagram and detailed descriptions of processes for understanding the fluidic system work.

  • Page 46: Flow Diagram Of Measurement

    Servicemanual Flow diagram of measur ement START BLANK START Meas. Needle to SR Sample in Needle piercing Cleaning Sampling Preparing for processes making dilutions Needle washing Generating (outside) HGB blank Vacuums SV to CP Generating Dilutions in parallel Needle Pre- WBC, MIX, 4diff Vacuums washing (Inside)
  • Page 47 Hemolyzer 5 Cleaning Making 4diff Needle (Inside) dilution + measurement refilling sheath puffer Generating vacuum SV to NP measurement + reset Cleaning 4diff measurement measurement MIX, RBC chambers, TCU loops 1  time Taking BASO Regenerate sample vacuum SV to CP...

  • Page 48: Initialization Of The Fluidic System

    Servicemanual 5.3 Initialization of the Fluidic System Fluidic initialization process performs the following steps: Checking the hardware conditions of the unit. • Checking of pump and pressures sensor by generating measuring vacuum. • Positioning all mechanical components by scanning moving range (with end- •...

  • Page 49: Needle Washing Processes

    Hemolyzer 5 5.7 Needle washin g proc esses When the primary sample is taken out, the needle will be washed outside by the wash-head using diluent and continuous drawing. During Diluting process, the needle will be washed through twice. First a preliminary flow of diluent slowly washes out the remnant of the whole blood into the washing head.

  • Page 50: Rbc Counting Process

    Servicemanual In the TCU unit, there are loops for lyse, stopper, blood + lyse, pre-diluted blood mixture. Temperature of these loops is controlled accurately.  After the 4-diff primer sample is pre-diluted and its temperature has been set, 3 syringes (lyse, stop, diluent) make the precise dilution through the TCU unit, the Shear Valve 4-diff sample loop, ending in the big vacuum puffer.

  • Page 51: Chamber Draining Processes

    Optical head and sampling line washing with sheath fluid 5.15 Standb y pro cess Because of the high throughput of the Hemolyzer 5, the measuring cycle ends with empty WBC chamber, and leaves the BASO sampling line is not cleaned. During continuous measurement the beginning of the next measurement will automatically clean this loop.

  • Page 52: Wake Up Process

    Servicemanual 5.16 Wake up process In this case the instrument prepares itself for the upcoming measurement cycle. Cleaning the wash head and calibrating the blood sensor. 5.17 Shutdow n proc ess The fluidic shutdown performs the following steps: Priming chambers with diluent to avoid drying out of aperture and prevent the chamber from contamination.

  • Page 53: Adjustments

    Hemolyzer 5 6 ADJUSTMENTS For proper functioning, it is vital to adjust the system correctly. Although Analyticon production sets the system in house, sometimes these adjustments needs to be done on the field also. Both mechanical and software adjustments has to be done properly to avoid measurement results or pneumatic errors.

  • Page 54: Set Opto Flags For Horizontal Movement

    Servicemanual The third rod is squire shaped and responsible to hold the vertical belt for the needle. The front of this rod is not fixed in a metal block, it “hangs” free. Guide Square shaped Guide rod Test the movement of the needle to Front or Rear positions in Service testing menu. If there is any grinding noise because of the improper lubrication, these three rods has to be lubricated.
  • Page 55 Hemolyzer 5 Opto flags Opto LEDs Opto Fixing Front wall Use handmade horizontal positioning of the needle, place the needle to the correct position, then align the opto flag to the opto sensor, see that the indication LEDs are turned on when the flag reached the sensor.

  • Page 56: Xy Vertical Adjustment

    Servicemanual Needle holdin block Washing Washing head  AS dockin Tube The opto sensors are fixed to the XY PCB and cannot be adjusted. 6.1.2 XY vertic al adjust ment This setting is necessary for the vertical motor movements because this adjustment sets the opto end-switches of the XY unit.

  • Page 57: Adjusting The Needle's Vertical Position

    Hemolyzer 5 position and hits the needle's vertical block, while the opto sensor still didn't received the 'Up' position signal. 6.1.2.2 Adjusting the needle’s vertical position The problem can be easily set by losing the two screws behind the needle and let the needle "release"...

  • Page 58: Needle Setting

    Servicemanual ‘Ok’. The system will pull the needle and the washing head to Up position. Now the system holds the XY unit in the Up position until the setting is r eady. The washing head is in position if the distance between the top of the head and the metal block above it is close to 1mm. 1 mm gap This distance is not strict, but it’s enough to provide space for the washing head when the needle moves.

  • Page 59: Software Made Setting

    The setting of the mechanics and the opto sensors are made by Analyticon during production, sometimes these alignments has to be checked or realigned.

  • Page 60: Shear Valve Lower Disc Position Setting

     After the lower disc is aligned, replace the upper disc and fix the thumb screw back to position. 6.2.2 Shear valve upper dis c settin g The upper disc and the mechanical stoppers (bumpers) are aligned by Analyticon during QC and End of Line procedures. If this setting is compromised, the mechanics should be aligned...
  • Page 61 Hemolyzer 5 again. To have access to the mechanical stoppers, the metal cover of the shear valve should be removed. The cover is fixed to its place by the two inner screws behind the thumb screw. The shear valve’s task is to change position to guide the sample and the reagents to the correct direction.

  • Page 62: Setting The Opto Sensors Of The Shear Valve

    Servicemanual The paper clip has a size and straight part just enough to push through the ceramics. Opto Opto Paper clip in alignment  Alignment The lower disc has two holes to test both positions. Test one position at a time. If the paper clip can go through both discs, it means the shear valve is in position.

  • Page 63: Amplifier Offset Setting

    Hemolyzer 5 The sensors are connected to a LED, which turn on when the sensors are triggered. Use these LED to check alignment. Turn the shear valve clockwise to the outer (chamber) position. Make sure that the shear valve is in the maximal position and cannot turn more. Put the paper clip to the alignment hole.

  • Page 64: Blood Sensor Calibration

    LSDACQ. If Auto-offset is not working or values are not close to zero, replace the LSDACQ. 6.4 Bloo d sensor calibratio n Hemolyzer 5 contains a blood sensor to ensure that the value of the sample is the same during every measurement. After every startup, the system calibrates the blood sensor automatically by making a Wakeup process.
  • Page 65 Hemolyzer 5 Field to enter number of test functions Checkbox to enable or disable blood sensor Press MDA to view MDA screen If the number of steps are the same for two times (out of three), the sensor calibration is successful.

  • Page 66: Sample Position Setting

    The purple area is a schematic representation of the purple area on the shear valve in the Hemolyzer 5. Please locate this tube in the analyzer (the tube is connected to the V41/1 tube in the vertical tube organizer.

  • Page 67: Pressure Sensor Adjustment

    Hemolyzer 5 If the bubble is not arrived to the correct position, press Cancel to make the system • (the dilutor) pulls the bubble higher from the shear valve. The system will ask to check every new position after Cancel is pushed.

  • Page 68: Set Serial Number

    Every laser head has unique values which have to be set in the instrument for proper working. These values were set by Analyticon, but has to be set again if the laser head was replaced. In Auto Alignment screen it's possible to check or overwrite the values. The Read DC button and the laser values are accessible, but other options are password protected.
  • Page 69 Hemolyzer 5 2. In service/service calibration menu, set Scatter X and Scatter Y values to 1,1. Press  Accept when done. These values needs to be set to make a proper start for the laser to calibrate. 3. In settings/system/set menu, select Gravity X and Gravity Y columns on the right list, and put them to the used columns on the left list by pressing the left arrow.
  • Page 70 Servicemanual 4. Do a control measurement using a control blood. In the database find the results of the measurement. Check the Gravity X and Gravity Y values of the measurement and compare them with the Gravity values shown on the blood sheet of the actual control lot.
  • Page 71 Hemolyzer 5 Hemolyzer 5 7. 7. If measured Gravity Y If measured Gravity Y is lower is lower than 80% than 80% of Lot of Lot Gravity Y, raise high Gravity Y, raise high angle gain angle gain in in auto alignment menu to bring the measured Gravity Y value closer to Lot Gravity Y.

  • Page 72: Short Guide For Laser Values

    Servicemanual Servicemanual 6. 6.9. 9.2 2 Short gui Short gui de de for for laser laser values values This is a This is a short description to give information about the values and settings of short description to give information about the values and settings of the optical the optical head assuming that user understand the operation of the optical measurement.
  • Page 73 In auto alignment menu, there is a chance to set the optical head values, laser power, low and high angle gains. These values are set by Analyticon QC, but these values can be and high angle gains. These values are set by Analyticon QC, but these values can be modified to align the optical head operation to modified to align the optical head operation to the instrument.
  • Page 74 Servicemanual Laser power values (both for 4diff and Baso) indicate the current operating the Laser Diode. The higher the laser power value, the lower the current flows though the diode. If the power values are increased, the current will be decreased making the scatter smaller in both dimensions (X and Y).

  • Page 75: Verification Procedures

    Hemolyzer 5 7 Verificatio n procedures 7.1 Self-test The analyzer has built in test function to check and evaluate operation of internal modules and systems. The function is accessible from the Main Menu, Diagnostics, Self-test function. There are two subsets of tests: electronic and pneumatic. Each process takes approximately 1 minute, and provides results of each tested parameter.

  • Page 76: Hemolyzer 5 Self-Test Guide

    Servicemanual 7.1.1 Hemolyzer 5 Self-Test Guide The Hemolyzer 5 Self-Test provides information about the actual status of hardware and software of the analyzer. This guide is intended for helping service engineers to correctly interpret Self-test result and to provide guidelines in troubleshooting the instrument.
  • Page 77 Hemolyzer 5 Laser DC level: Laser DC (Dark Current) is the reference value of the optical • measurement circuit. Measurement is performed on diluent. When the optical flow cell is clean and bubble-free, laser light isn’t scattered by anything and only a very small signal should be measured by the detectors.

  • Page 78: Pneumatic Self-Test Parameters

    Servicemanual DO NOT CHANGE THIS VALUE UNLESS IT'S NECESSARY OR YOU INSTALL A NEW LASER HEAD IN THE ANALYZER. IN THAT CASE LASER POWER VALUE TO BE SET IS WRITTEN ON THE AUTOALIGNMENT SHEET OF NEW LASER HEAD OR ON THE SIDE OF THE OPTICAL HEAD.
  • Page 79 Hemolyzer 5 valves, and pressure sensor board, run pressure offset adjustment and repeat S elf- test. Noise/Pulse: This is an electronic test but it require diluent to be present in the • counting chambers. pls/8 sec: if failed either electronic noise is picked up by the electrode or the amplifier •...

  • Page 80: Error Messages

    Servicemanual 7.2 Error Messages The analyzer checks the operations of several mechanic, fluidic and electronic parts during measurement. The system shows the type of the error on the LCD display if any kind of malfunction is detected. When an error message pops up, the details contains the processes were involved when the error appeared.
  • Page 81 Hemolyzer 5 EG_NEXT_PIERCE EG_INIT_PORT EG_INIT_FPGA EG_INIT_PIC EG_INIT_MDATA EG_LOOP EG_SAMPLER_DELEG EG_MOVING_DELEG EG_NMOVING_DELEG EG_COMM_PARAM EG_TEST_DISLPAY EG_INIT_STATE EG_FILE_NOT_FOUND EG_FILE_NO_INFORMATION EG_FILE_OPEN EG_GET_FILE EG_SEND_FILE EG_CRC_CREATE EG_UPGRADE EG_TEST_SPEC EG_ANSWER EG_COMMUNICATION EG_DRAIN EG_NEEDLESETTINGS EG_HARDCLEANING EG_MAININIT EG_WAITFORCONTROLEVENT EG_S_MSGMLR_TIMEOUT EG_NM_MSGMLR_TIMEOUT EG_M_MSGMLR_TIMEOUT EG_PRESSURE EG_THREADS EG_WAITFOREVENT EG_SENDEVENT EG_DELETE_FILE EG_SETTINGS EG_REAGENTS EG_SAMPLER_CONSTRUCTOR...
  • Page 82 Servicemanual EG_SAMPLER_CANCEL_WAITSTATE EG_SAMPLER_WAITSTATE EG_SAMPLER_ONLINE EG_SAMPLER_SAMPLERDY EG_SAMPLER_ERROR EG_SAMPLER_FATALERROR EG_SAMPLER_SENDPOS EG_SAMPLER_BARCODE EG_SAMPLER_INITDONE EG_SAMPLER_STOPPED EG_SAMPLER_TRAYDONE EG_SAMPLER_PONG EG_SAMPLER_SAMPLEMIX EG_SAMPLER_BAROK EG_SAMPLER_SENDTRAYINFO EG_SAMPLER_SENDRACKINFO EG_SAMPLER_UNKNOWNCMD EG_SAMPLER_CMD EG_SAMPLER_TRAY_DONE EG_SAMPLER_STARTPOS EG_AUTOSAMPLER_MEASURE EG_FILL EG_TEST_PIERCE EG_MOVE_DILUTOR_1 EG_MOVE_DILUTOR_2 EG_MOVE_DILUTOR_3 EG_MOVE_DILUTOR_4 EG_BOB EG_HORIZONTAL EG_VERTICAL EG_DILUFULL EG_GET_BUBBLE EG_BUBBLING EG_MICRO EG_MEAPUFF EG_MEAPUFFBIG EG_MEAPUFFSMALL EG_PREDIL EG_DIL_L EG_SAMPLE_MOVE EG_NEEDLE_WASHING EG_WBC_STOP...
  • Page 83 Hemolyzer 5 Hemolyzer 5 EG_CHAMBER_WBC EG_CHAMBER_WBC EG_HGB_MEASURE EG_HGB_MEASURE EG_BASO_MEASURE EG_BASO_MEASURE EG_BASO_SAMPLE EG_BASO_SAMPLE EG_RBC_MEASURE EG_RBC_MEASURE EG_WASHING_DIFF EG_WASHING_DIFF EG_PREDRAIN EG_PREDRAIN EG_SENDTHEEND EG_SENDTHEEND EG_WBC_MEASURE EG_WBC_MEASURE EG_OPT_SAMP_CL EG_OPT_SAMP_CL EG_SET_POSITION EG_SET_POSITION EG_BURNRBC EG_BURNRBC EG_BURNWBC EG_BURNWBC EG_TIMEOUT EG_TIMEOUT EG_TOTTI EG_TOTTI EG_AAMVERT EG_AAMVERT EG_AAMHORI EG_AAMHORI EG_AUTO_ALIGNEMENT EG_AUTO_ALIGNEMENT...
  • Page 84 Servicemanual Servicemanual EG_CELL_CLOGGING EG_CELL_CLOGGING EG_FILL_LASER EG_FILL_LASER EG_DEBUG EG_DEBUG EG_REPAIR EG_REPAIR EG_CANCEL_ANSWER EG_CANCEL_ANSWER EG_CANCEL_AT_END EG_CANCEL_AT_END EG_PIC_ERROR EG_PIC_ERROR EG_SET_OFFSET EG_SET_OFFSET EG_AS_INFO EG_AS_INFO EG_REAGENT_LOCK EG_REAGENT_LOCK EG_RV_RD_ERROR EG_RV_RD_ERROR EG_RV_WR_FAULT_ERROR EG_RV_WR_FAULT_ERROR EG_RV_WR_PROTECT_ERROR EG_RV_WR_PROTECT_ERROR EG_RV_ARG_ERROR EG_RV_ARG_ERROR EG_RV_UNEXPECTED_ERROR EG_RV_UNEXPECTED_ERROR EG_RV_KEY_ERROR EG_RV_KEY_ERROR EG_RV_CRC_ERROR EG_RV_CRC_ERROR EG_RV_PERMISSION_ERROR EG_RV_PERMISSION_ERROR EG_RV_DEVICE_MISSING_ERROR EG_RV_DEVICE_MISSING_ERROR EG_RV_NOT_ENOUGH_MEASUREMENTS EG_RV_NOT_ENOUGH_MEASUREMENTS EG_SERIALS...
  • Page 85 Hemolyzer 5 Hemolyzer 5 7.3 Se Serv rvic ice e Menu Menu There is a There is a Service Service  menu for servicing and operation checking purposes; however it is hidden  menu for servicing and operation checking purposes; however it is hidden from the standard user.

  • Page 86: Service Menu

    Servicemanual Once you typed in the correct service code, you will enter into the service menu. The system is designed to provide you continuous access to service functions: you will have to enter the service code once for a specific service session. The button in the lower right corner says “Service mode OFF”.

  • Page 87: Service Functions

    Hemolyzer 5 7.3.1 Servic e func tio ns...

  • Page 88: Test Functions

    Servicemanual 7.3.1.1 Test functions This item allows running specific test procedures. You have to enter the “ID” of the required service and RUN the process. The list of available functions and their expected result is listed below. Name Expected result Go to Standby The system will go into standby independently of standby timer status...

  • Page 89: Network Management

     Allows selection of RAW (unprocessed) data file save mode. Raw data files can be important for Analyticon support or R&D team to analyze and troubleshoot. Turn on Raw data saving mode to make the system save measurement data during further measurements.

  • Page 90: Small Sample Mode

    Servicemanual 7.3.1.15 Small sample mode Enables to use small sample mode if it's installed into the system. 7.3.1.16 Pre-diluted mode Enables to use pre-diluted blood sample. 7.3.1.17 Monitoring  Allows to connect the system to an external computer by entering its IP address. If monitoring is enabled, it's possible to see how the unit is used in real time on a PC or laptop which is located in another area.

  • Page 91: Service Calibration

    Hemolyzer 5 7.3.3 Servic e calibr ation The analyzer provides a menu for Service calibration purposes. This screen displays the actual calibration factors, service level calibration factors and the user level calibration factors. Service and User level factors are multiplied and the system is using the result as the actual calibration factor for each measured value.

  • Page 92: Accept User Factors

    Servicemanual 7.3.3.5 Accept User factors Pushing this button will reset the User factor values to 1.00. Reset values will be multiplied with Service factors, creating new Service factors. 7.3.3.6 Back Returns to Service menu screen. 7.3.3.7 Accept  Actual calibration factors can be modified by tapping the touch screen. After new values are typed in, Accept has to be pushed to make the system use the new factors.

  • Page 93: Auto Alignment

    Hemolyzer 5 7.3.5 Auto alignm ent This function allows access to adjusting the optical head’s laser alignment. The page is password protected, but some functions are accessible without entering the password. Laser settings can be typed in manually. •  After values are entered, press Save values to confirm the settings.

  • Page 94: Set Up Barcode Reader

    Servicemanual 7.3.6.6 Set up Barcode Reader 7.3.7 Adjustments This page contains software and mechanical adjustments. These adjustments and values have to be set correctly for proper working. Optical channels offset • Reduces the offset voltages in the optical head. Can be set by hand or automatically.

  • Page 95: Multiuser Settings

    Level software in the DimmBoard. Regularly, communication and pneumatic procedures post status report on this screen.  Analyticon Support may ask you to open this window and report messages. MdaOn checkbox has to be enabled to see the activity of the Low Level software.

  • Page 96: Software Upgrade

    The software of the Instrument can be upgraded using a commercially available USB flash memory device. Hemolyzer 5 software can be upgraded with software releases from Analyticon. It is recommended to install both upper (Windows XP) and lower (DimmBoard) software from the same release package to keep maximum performance and compatibility.

  • Page 97: Reagent Lock

    Hemolyzer 5 The Auto-Sampler has software similar to the Low Level software in the DimmBoard. This software can be controlled by the High Level software. It is possible to update the software here is new software is enable. 7.3.11 Reagent Loc k...

  • Page 98: Qc Wizard

    Servicemanual 7.3.12 QC Wizard It's possible to run several automatic tests on the analyzer. QC Wizard is used by Analyticon production to ensure that all mechanical, pneumatic and electrical settings are correct. QC wizard requires a 'lim' file containing ranges for all the tests.

  • Page 99: Software Systems

    Hemolyzer 5 7.4 Software syst ems Hemolyzer 5 has a complex software system. This system can be divided into two parts, low level system and high level system. The low level system (DimmBoard SW) is responsible for the moving parts of the analyzer and the high level system is responsible for the user interactions.
  • Page 100 Servicemanual checkbox next to the file, then clicking OK. The process will start. Click “next, next…install”. Wait for the process to end. • Click on “Finish” (the new SW will start). • Note: Software restart (especially if there are more than 1000 samples present in the database) might take several minutes.

  • Page 101: Install Operating System Windows 2.0 Install Using Pendrive

    Hemolyzer 5 7.5.1 Install operating syst em Window s 2.0 inst all usin g pendri ve Tools needed: Pendrive (at least 4 GB) Pendrive with high level software Windows iso image file 2.0 (winpe_x86_v2.0) Rufus v1.2.0(or higher) application Additional files directory External usb keyboard and mouse Step 1.
  • Page 102 Servicemanual Step 4. Let „Rufus” copy the ISO to the pendrive. It will take approximately 80-120 minutes, depending on the hardware. Do not remove the pendrive during the copy! Step 5. When copying if finished, you have to select the files in the  Addi ti on al f il es directory and copy it to the...
  • Page 103 Hemolyzer 5 Step 6. Now we have to set the hematology analyzer to recognize to Pendrive. Insert the pendrive to the hematology analyzer, also attach the USB keyboard and turn the analyzer on. While the booting process is going, press „del” button to enter to BIOS.
  • Page 104 Servicemanual Step 7. available. In this case, remove the  and type in the bootable pendrive below commands into the console to create proper partition on the HDD. DISKPART SELECT DISK 0 CLEAN CREATE PARTITION PRIMARY SIZE=100000 FORMAT FS=NTFS QUICK  ACTIVE  ASSIGN LETTER = C CREATE PARTITION PRIMARY FORMAT FS=NTFS QUICK...

  • Page 105: Installing A Printer

    7.5.2 Installi ng a Print er Hemolyzer 5 is using a Windows operating system. To add a new printer on Hemolyzer 5 you have to install the printer specific driver. The operating system has limited possibilities for user interaction affecting operating system functions. Follow the steps below t o install a printer, or use one of the printer install functions from the service menu (see section  7.3.13).

  • Page 106: Installation

    If you decide to route the reagent tubing through the tabletop, please ensure that any necessary holes are drilled before the installation process starts. If you plan to connect the ‘Hemolyzer 5’ analyzer to any external devices (keyboard, mouse, printer, host computer, etc.), please ensure that all necessary preparations (cable-channels,...

  • Page 107: Gather Your Peripherals Devices

    5’ analyzer. Safely move the ‘Hemolyzer 5’ analyzer, the accessory box, and the optional  Autosampler (if ordered) and the Autosampler accessories to the selected location. Keep the ‘Hemolyzer 5’ analyzer in an upright position and gently set it down in its new location. 8.3.2 Visual Inspectio n Visually inspect the ‘Hemolyzer 5’...

  • Page 108: Connect The Optional Auto-Sampler

    2. Figu re: Shear Valve Prot ectiv e Card 8.3.4 Connect the Option al Auto-Sampler To connect the optional Auto-Sampler to the ‘Hemolyzer 5’ analyzer, perform the following steps: Unscrew the five screws and remove the small cover plate on the right side of the •...

  • Page 109: Connect The Power Cord

    Please read reagent descriptions carefully for possible emergency actions. 8.3.6 Connect the Power Cord Before connecting the power cord, make sure that all of the ‘Hemolyzer 5’ back panel switches and the optional Autosampler switches are turned off:...

  • Page 110: Verify The 'Hemolyzer 5' Computer Operation

    8.3.7 Verif y the ‘Hemolyzer 5’ Computer Operation We are now going to start up, then shutdown the ‘Hemolyzer 5’ analyzer to ensure that the computer system and software start and shut down correctly. To power and shut down up the ‘Hemolyzer 5’ analyzer, perform the following steps: Turn on the main power switch on the rear panel of the ‘Hemolyzer 5’...

  • Page 111: How To Install A Printer

    Windows recognizes the newly attached hardware and it starts the installing process automatically by popping up the install window. If you press the touch while the printer install screen is loading, the window will hide behind the 'Hemolyzer 5' software. Use ALT+TAB to switch between the two screens in the popped up printer install screen, browse the driver files from the pendrive •...
  • Page 112 Servicemanual Make sure that the cover of the Autosampler is closed and that the ‘Cover’ led • changes to green; Double click the AS state on the left side of the Status Bar at the bottom of the screen • to bring up the ‘Autosampler info’...

  • Page 113: Using The Settings Menu

    'Hemolyzer 5' analyzer supports uploading of measurement data to an LIS or host computer. If your laboratory has an LIS system, the ‘Hemolyzer 5’ analyzer can be connected to it using a serial or an Ethernet connection. An LIS system is not required to operate the ‘Hemolyzer 5’...

  • Page 114: Set Up A Serial Lis Connection

    Servicemanual 8.3.13 Set Up a Seri al LIS Connect ion To connect the ‘Hemolyzer 5’ analyzer to an LIS system using a serial LIS connection, perform the following steps: Ensure that your LIS system is compatible with the Analyticon 3.1 protocol using a •...

  • Page 115: Set Up An Ethernet Lis Connection

    Analyticon certified service engineer can complete the installation. To connect the ‘Hemolyzer 5’ analyzer to an LIS system using an Ethernet LIS connection, perform the following steps: Ensure that your LIS system is compatible with the HL7 version 2.5 protocol or higher...

  • Page 116: Checklist For Installing Hemolyzer 5 Hematology Analyzer

    Servicemanual Lastly, it's possible that a calibration procedure is needed on the ‘Hemolyzer 5’ analyzer. See section 11 in User Manual for additional details regarding ‘Hemolyzer 5’ calibration. 8.4 Checkl ist fo r installin g Hemolyzer 5 hematolog y analyzer 1.
  • Page 117 22.7.9 in User’s manual. 15. Run a control blood in ’Control’ mode or run QC procedure. 16. Check if results are within the acceptable range. Run calibration if necessary. 17. The Hemolyzer 5 is ready for routine measurements.

  • Page 118: Troubleshooting

    Servicemanual 9 Troubleshooting This chapter will show how to do a step by step troubleshooting on the 'Hemolyzer 5' analyzer. Following the troubleshooting process ensures that the problem can be identified without making any harm on the system. This chapter will helps find the root cause of the problem and navigate to the page where you can find information how to solve the problem.

  • Page 119: Powering Up The System

    Analyticon, but in some cases (windows recovery, high level sw reinstall) this setting has to be done again or at least has to be checked. 'Hemolyzer 5' analyzer has two software levels. The High Level Software is responsible to communicate with the user and to give the user's commands to the Low Level Software.

  • Page 120: Checking The Hardware Adjustments

    The proper settings of the mechanical parts are important to make good measurements and to increase the lifetime of the system. The mechanics are aligned by Analyticon during production but some realignment may need when some mechanical parts are replaced or repaired.

  • Page 121: Run A Self-Test

    Hemolyzer 5 4. Press 'Rear' and 'Front' buttons at MHori test to move the needle back to the Auto- Sampler station and back to sample rotor position. Listen the sound of the mechanics, if there is a grinding noise or a pneumatic error, please see section 6.1.

  • Page 122: Run Control Measurements

    Servicemanual PrV RBV PrV WBC   Warnings Gravity X Gravity Y Woc 4 Woc B Select these columns and add them from the right to the left column block then press Accept and Save. If you don't press save the setting were not made and the result columns will not appear in the database.

  • Page 123: Mechanical Problems

    Hemolyzer 5 Cell numbers (Woc 4, Woc B) are proper on normal control. Woc numbers (White blood cell Optical Count) indicates how many cells are present during optical measurement. These values have only minimum number. Woc 4 min : 2.72 ...

  • Page 124: Sample Rotor (Sr Or Bob) Failures

    Servicemanual Test the motor with the built in functions in service testing menu. It will always try to • operate all sensors, and will drive the motors with necessary current and power. Any Setting or adjusting (mechanical, opto sensor) should be done only after the •...

  • Page 125: Shear Valve (Sv) Related Errors

    Hemolyzer 5 The motor gives a strange noise (not “smooth”) even when moving. Check cable or • connection problem The problem comes up only when using Stastedt (or similar) sample tubes from • sample rotor. This could happen because the system is set to pierce a monovette type of tube, not a Stastedt or the incorrect setting of opto flags on horizontal movement.

  • Page 126: Dilutor Errors

    Servicemanual 9.2.5 Diluto r errors 9.2.5.1 Dilutor is making noise Dilutor pistons are not attached correctly to the dilutor’s moving part. The piston has a • different size hole on the bottom; the larger hole has to face the instrument. One optosensor on the dilutor PCB is faulty.

  • Page 127: Electronics Related Problems

    Hemolyzer 5 There is not enough vacuum to fill up the reservoirs. Check all the valves in Service • Testing menu. Replace faulty or non-opening or closing valves. The pressure sensor is faulty. Check sensor offsets, see section 6.6. •...

  • Page 128: Communication Errors

    Servicemanual 9.3.2 Communi cation errors Check the communication by going into Service menu/Sw Upgrade and press Refresh button. If communication is ok, all the available firmware and software will be listed. If there is a problem with communication, the unit will send message “Pneumatic error” or “USB communication lost”.

  • Page 129: I 2 C Errors Displayed At Startup

    • interface SW does not start (just widows desktop appear). In this case the instrument doesn’t have a properly installed High Level Software. Contact the Analyticon support via support@analyticon-diagnostics.com to get the right software. Copy the software to a pen drive and insert it to the instrument. See software installation in section 7.5.

  • Page 130: Removing The Dimmboard

    Servicemanual in the flow cell, press valve #1 and #32 together and check the laser waste tube for bubbles. If there are too many bubbles in the laser, check the tubing and "T" connections in the tubing at valve #20 and #32. If bubbles are leaving the laser but not entering into it, the injector must be cracked.

  • Page 131: Maintenance

    The procedures in this section are service maintenance. Preventive maintenance frequency depends on the daily workload of the customer. In the Hemolyzer 5 User Manual and Service Manual the maintenance interval of moving parts and syringes is determined as to be done  or...
  • Page 132 Spare parts needed: Maintenance KIT for A5 Contains: Part number Descri ption Quantity  A5330 Washing head Hemolyzer 5  A543 4/1.8 Tygon tube, 1 m  A5433 1,27/2,23 Tygon tube (type: S 54-HL)  A5439-S 0,78/2,38 Tygon sampling tube for A5, 26 cm  A5-AJN014 Piercing needle (Hemolyzer 5)  AP251...
  • Page 133 Hemolyzer 5 RBC/WBC Chamber J5P421 RBC/WBC Aperture J5P431 -  2/2 Valve with Coil A504 3/2 Valve with Coil A505 -  Pressure sensor board A5519 Preventive Maintenance Protocol : 1. First steps / Checking the state of the analyzer 1.1 turn on the analyzer, run background and check if values are within accepted range 1.2 run Self-test (electronic and pneumatic), if it is „successful”...
  • Page 134 5.3 cycle dilutors from service menu again, if any of the syringes does not seal properly (air bubbles are still getting inside near the plunger) replace the syringe(s)  Analyticon recommendation as preventive maintenance: replace syringes after 50.000 measurement cycles 5.4 clean dilutor optosensors from dust by means of compressed air 5.5 clean the driving pin of dilutors then lubricate them with teflon grease...
  • Page 135 “flow cell cleaning” procedure option was done. If „laser off” DC level is high that might indicate failure of a component of the laser head. Contact Analy ti co n Supp or t Team ( support@analyticon-diagnostics.com ) for further information and assistance.
  • Page 136 Servicemanual  Analyticon recommendation as preventive maintenance: change the in-line mixer tubes once per year. 8.4 reassemble the TCU unit to its place, follow instructions of the SM Proceed to step 9. 9. Wash head and XY bloc k 9.1 clean the sliding bars of the XY unit with a paper towel and apply a thin film of teflon grease on them 9.2 clean the optosensor on the XY opto board with compressed air...

  • Page 137: Flow Cell Cleaning

    Hemolyzer 5 10.3 Flow cell cleanin g The optical measurement system o f t he Hemolyzer 5 is s tric tly vacuum-based. Please do not apply pressure to the flow cell! Tools needed: small flat screwdriver • Syringe(10-15 ml or bigger) •...
  • Page 138 Servicemanual 6. Pour Hypoclean CC or filtered bleach into a cup and place the end of the sheath tube in it. 7. Gently pull the syringe to make the bleach flow through the Flow Cell. You can increase cleaning efficiency if you let air bubbles in the sheath tube while pulling.
  • Page 139 Hemolyzer 5 10. Remove the laser sample tube from upper disc of SV. This is the thinnest tube. Use the small flat screwdriver to help removal from metal port. 11. Place the end of sample injector tube in bleach. Gently pull the syringe to clean the sample injector tube from the inside.

  • Page 140: Removal And Replacement Procedures

    Servicemanual 11 Removal and replacement procedures 11.1 Opening the ins tru ment Both side covers of the instrument can be easily removed. This allows reaching of the fluidic system and the mechanical parts. Other parts of the analyzer can be reached by lifting up the front panel.

  • Page 141: Shear Valve Assembly

    Hemolyzer 5 11.2 Shear Valve Ass embly Shear Valve Assembly is located in middle of front side of Hemolyzer 5 behind the front cover. Main parts of Shear Valve Unit: Ceramic valve • Driver unit with a stepper motor •...

  • Page 142: Xy Unit

    Servicemanual Shear Valve Opto Sensor board replacement Shear Valve opto sensors are mounted to the right • side on Shear Valve Assembly with 3 screws in such a way the sensors can be set separately. remove screw behind the circular opening (through •...
  • Page 143 Hemolyzer 5 Sampling needle is sharp and represents biohazard! Take extra care and wear gloves when • servicing this part of the analyzer. Remove the baluster. Its lower screw can be access from the bottom of analyzer. • Locate and unscrew the four screws •...
  • Page 144 Servicemanual Gently remove the wash head holder • with the wash head, by twisting it clockwise and pulling it down until released. Disconnect electrical cables • Remove the XY unit • Sampling needle fixing screws Upper MVert Washing head and washing head MHori Sample Rotor basis...

  • Page 145: Sample Rotor

    Hemolyzer 5 11.4 Sampl e Rotor Sample rotor holds the sample tube and turns it inside the instrument for cap piercing. In order to provide its proper operation with XY unit, they are equipped together. Sample Rotor removal: Remove XY unit with Sample Rotor as it is written •...

  • Page 146: Dilutors

    Servicemanual 11.5 Dilutors Hemolyzer 5 has two separate main dilutor modules, located behind the front panel on the lower part of the front plate. Each modules is secured by four hex screws and has a f lat cable connection to PPB1 respectively PPB2. The removal/replacement procedure is as follows: 1.

  • Page 147: Tcu Module

    Hemolyzer 5 Correct Incorrect 11.6 TCU Modul e The Temperature Control Unit is located below the Laser Head Assembly. It is connected to the Shear Valve and to the tube organizer via Tygon tubing. The internal electronics are connected to the power supply and to the LSDACQ board. In order to remove this unit, follow...

  • Page 148: Valve Block

    Servicemanual remove the 4 screws in the back of the analyzer securing the rear open the assembly plate on the left side of the analyzer to have access to the TCU control board cables locate and disconnect the power cable of the TCU module disconnect the controlling cable coming from the LSDACQ board...

  • Page 149: Pre -Amplifier Board

    Hemolyzer 5 The tubes are connected to the valve pins. The coil is soldered to valve block board.  A plate fixes the main parts together on the coil site. Pull with pliers To remove individually you’ll need to use a plier to pull the valve fixing plate out at the back and after that it is possible to remove only the valve itself.
  • Page 150 Servicemanual Pull off the connected tubes from inner fittings of Waste output and pump inlet tubes from lower right side of flow Panel. 5. Pull off the connected cables (red-green-black) from the PPB boards. The pump 1 (outer one) power cable is connected to PBB1 (left board), the pump 2 (inner one) power cable is connected to PBB2 (right board).

  • Page 151: Hardware Module

    Hemolyzer 5 11.10 Hardw are Module The whole removing procedure is easier by opening the front cover and with removed side covers. 1. Unscrew the five screws connected on the rear plate. 2. The assembly is positioned and pushed down inside by a spring sheet. Gently pull out the fixing plate until almost the whole module comes out.

  • Page 152: Laser Head Assembly

    Servicemanual 11.11 Laser Head Ass embly To remove t he Laser Head: Turn on the analyzer. 1. Press and hold the Menu  icon until the on-screen keyboard pops up, then type in the service password. Service  button appears on the main screen, click on it to enter the service menu, then click on  and wait until the...
  • Page 153 Hemolyzer 5 8. CLOSE FRONT DOOR 9. Disconnect both cables from the optical head. Refer to picture 4 below. (#1 – autoalignment cable*; #2 – optosensor board cable; #3 – laserdriver board cable) Note: it is easier to disconnect the laserdriver board cable from the LSDACQ board. Units...
  • Page 154 Servicemanual 10. Locate the four holding screws of the base-plate of the optical head. Refer to Picture 5.below. Use a 2,5 mm hex screwdriver to remove the screws. Take extra care of the washers. 11. Gently remove the optical head unit. To avoid damage of the sample injector ...

  • Page 155: Reinstalling The Laser Head

    Hemolyzer 5 11.11.1 Reinst alli ng the Laser Head: (Analyzer is turned off, right side and top covers removed, front door is CLOSED) 1. Connect laser driver board cable to laser head. 2. Place the Laser Head to its’ place. Guide tubes to exit towards the front.

  • Page 156: Wbc Aperture Removal

    Servicemanual 11.12.2 WBC apertu re removal: Drain the chambers in • maintenance menu. Unscrew hex nut on the right site • of front panel that fixes the black metal shield. Remove the black shield. • Remove four screws from the unit. •...

  • Page 157: Hgb Head Removal

    11.13 Displ ay uni t Display unit is installed on to the front panel of Hemolyzer 5 with a metal mounting unit. The LED display is controlled directly by Hardware block, the touch screen is driven by a Touch Controller board. It is behind the display, under a metal cover.
  • Page 158 Servicemanual Display removal: Turn the system off • Remove the front cover fixing arm and two hinges • Disconnect the grounding cable • Take off the front panel and put it on flat surface • Remove the metal cover of Touch Controller board •...

  • Page 159: In Chamber Position

    Hemolyzer 5 11.14 Hemolyzer 5 Fluidi cs v2.1 - SV in Chamber Posit ion...

  • Page 160: In Needle Position

    Servicemanual 11.15 Hemolyzer 5 Fluidi cs v2.1 - SV in Needle Position...

  • Page 161: Tubings

    Hemolyzer 5 11.16 Tubings Preparing of tubings: (A556) (A546) (A545)   V2/2 V15/1 V12/2 PUFFER V12/3 V1/3 V13/2 V12/1 V4/3   V6/ 3 V8/ 3 V3/3 V5/3 V7/3 V23/2 V10/1 Cham   SVF12 BASO Puffer  2   2   V15/2 V9/1 V11/1...
  • Page 162 Servicemanual DILU I. DILU II. PUFFER PUFFER V36/1 V43/1 18,5 V42/1   V40/1 V41/1 V37/1 V29/2 V42/2 V10/2 V9/2 PUFFER V34/2 V3/2 V35/2 MEA Puff Small PUFFER Dilu I. Puffer  Puffer  4    1  0   Small   V23/1 V8/2 Dilu II  1   0 Puffer ...
  • Page 168 Servicemanual Ly se   Lyse -E O - EO...
  • Page 170 Servicemanual...
  • Page 171 Hemolyzer 5 Note: 400cm tube from V20 to SV upper disc is type A5433 1.27/2.23...

  • Page 172: Appendix

    Servicemanual Appendix 12.1 Recomm ended kit of too ls PC standard keyboard (USB) •   Screwdrivers: • Slot Screwdrivers Cross Slot Screwdrivers (Philips) Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes) Pocket digital multimeter • Diagonal Cutter (plier) •   Nipper •...

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