Teledyne Photometrics KINETIX Manual

Teledyne Photometrics KINETIX Manual

10 megapixel 6.5 um pixel size 500 frames per second 29.4 mm field of view 95% quantum efficiency
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Summary of Contents for Teledyne Photometrics KINETIX

  • Page 2: Limited Warranty

    Teledyne Photometrics warrants this product against substantial defects in materials and/or workmanship for a period of up to three (3) years after shipment. During this period, Teledyne Photometrics will repair the product or, at its sole option, repair or replace any defective part without charge to you. You must deliver the entire product to the Teledyne Photometrics factory or, at our option, to a factory-authorized service center.
  • Page 3: Limited One (1) Year Warranty On Refurbished Or Discontinued Products

    During this period, Teledyne Photometrics will repair or replace, at its sole option, any defective parts, without charge to you. You must deliver the entire product to the Teledyne Photometrics factory or, at our option, a factory-authorized service center. You are responsible for the shipping costs to return the product to Teledyne Photometrics.
  • Page 4: U.s. Government Restricted Rights

    In no event shall Teledyne Photometrics’ liability exceed the cost of the repair or replacement of the defective product or part.
  • Page 5 58-723-004 Rev A01...
  • Page 6 58-723-004 Rev A01...
  • Page 7: Table Of Contents

    Host Computer Requirements ..............................4 Software Installation ................................5 Installing the PCI Express Interface Card ..........................5 Connecting Kinetix to PCI Express Bus ............................. 8 Connecting Kinetix with USB..............................10 Chapter 3 – Theory of Operation ............................11 Introduction ................................... 11 CMOS Image Sensor Structure ...............................
  • Page 8 Digital Binning ..................................14 Sensor Clearing ..................................14 Bias Offset Settings ................................14 Pixel Noise Filters ................................... 15 Chapter 4 – Operating Features ............................18 Introduction ................................... 18 Modes and Gain States ................................18 Clearing Mode ..................................18 Regions of Interest ................................. 19 Programmable Scan Mode ..............................
  • Page 9 Camera Running Too Warm ..............................30 PVCAM Error Message Appears ............................. 31 Lengthy Pauses During Imaging ............................. 31 Chapter 6 – Basic Specifications ............................32 Kinetix Dimensional Drawings ............................... 32 Camera Weight ..................................32 Sensor Specifications ................................33 Power Supply Specifications ..............................33 Appendix A –...
  • Page 10: Chapter 1 - Overview

    Chapter 1 – Overview About This The Kinetix Scientific CMOS Camera User Manual is divided into six chapters and three appendices. Teledyne Photometrics recommends you read the entire manual before Manual operating Kinetix to ensure proper use. The chapters that follow the Overview are briefly described below.
  • Page 11: Environmental Requirements

    Do not use a C-mount lens with optics that extend behind the lens flange. Environmental The Kinetix camera system should be operated in a clean, dry environment. The camera system’s ambient operating temperature is 0°C to 30°C with 80% relative humidity, Requirements non-condensing.
  • Page 12: Chapter 2 - System Installation

    Note: Carefully review the Precautions section in the previous chapter before performing any of the procedures outlined in this chapter. Again, use only a Kinetix PCI Express data cable and Kinetix PCI Express interface card with the camera. Using a different cable or interface card may result in unexpected errors or permanent damage to the system.
  • Page 13: Getting To Know Kinetix

    Getting to Highlights of the Kinetix camera are shown below. The Kinetix package includes the PVCAM drivers designed to allow you to use this camera with a variety of third-party imaging Know Kinetix software. For a list of supported software, visit the Teledyne Photometrics website.
  • Page 14: Software Installation

    Note: To achieve optimal performance, see our latest recommended PC specifications at https://www.photometrics.com/support/recommended-pc-specifications. Software An appropriate Quick Installation Guide is included as an insert with the Kinetix camera. This guide provides step-by-step instructions for installing the camera interface software Installation for Windows-based computers.
  • Page 15 Note: The model of PCIe card shipped with the camera may differ from the one shown in the photo. Before attempting to operate the camera, first install this interface card into the PC via the following steps: Shut down the PC. Unplug the PC from power mains and ensure the camera is turned off.
  • Page 16 Locate an available 8-channel or higher PCIe slot (marked x8). Refer to the PC’s documentation to locate a suitable slot. User Tip: The PC may have motherboard slot information on the side cover. Hold the PCIe card (being careful not to touch the board components or PCIe bridge pins) and insert it, properly orientated, into the open slot.
  • Page 17: Connecting Kinetix To Pci Express Bus

    PCIe card at the full speeds available. Connecting The two Kinetix camera data cables are identical, quick-insertion, quick-release cables that connect the interface card and the camera. One of these cables is shown below.
  • Page 18 For Kinetix builds June 25, 2021 (or later), camera serial numbers A21F723001 (or greater), it is no longer necessary to turn the camera on before powering up the computer. Kinetix can be turned on after the computer starts up and power cycled while the computer remains on.
  • Page 19: Connecting Kinetix With Usb

    Connecting The Kinetix camera’s USB 3.2 10 Gbps interface (formally known as USB 3.1 Gen 2) is ubiquitous and easy to use. To use the interface, the PC must have an open USB 3.2 or Kinetix with Thunderbolt port.
  • Page 20: Chapter 3 - Theory Of Operation

    Chapter 3 – Theory of Operation Introduction Backside-illuminated scientific CMOS (BSI Scientific CMOS) sensors are a recent but rapidly maturing development in image sensor technology. Boasting near-perfect 95% quantum efficiency (QE), they are able to provide the highest levels of sensitivity. New cameras can now leverage these high-QE sensors to offer a combination of low read noise, high full well capacity, and fast readout rates that is ideal for modern low-light imaging.
  • Page 21: Gain Combining And Bit Depth

    “gain combiner.” This mathematical operation is performed on the camera’s FPGA. The result is a single gain of approximately 0.25 e-/ADU. In practice, Kinetix offers both a combined 16-bit ADC output as well as 12-bit and 8-bit single ADC output. The 12-bit Sensitivity Mode uses the same approach as 16-bit Dynamic Range Mode but combines two high-gain measurements to reduce the camera read noise.
  • Page 22 (The row being digitized “rolls” through the sensor.) For Kinetix, the time to digitize a single row is 3.75 µsec in Dynamic Range Mode. Digitizing 3200 rows of pixels, the time delay from the top to the middle of the sensor is approximately 12 msec.
  • Page 23: Digital Binning

    4x and improve SNR by 2x as the noise from each pixel adds in quadrature. The Kinetix system includes symmetric and asymmetric on-camera digital binning of up to 4 x 4 pixels.
  • Page 24: Pixel Noise Filters

    The dynamic fluctuations must be detected and corrected with each acquisition. The Kinetix camera has noise filters for this purpose. Detection of a transiently high or low pixel value due to RTN is based on comparison to the median value of the neighboring 3x3 pixel array.
  • Page 25 “Dark” filters work on the low side of the local median, while “Bright” filters work on the high side of the local median. The filter is only applied if the pixel’s value exceeds (or is below) a threshold expressed as a percent of the local median x 100. For example, in the figure above, a Despeckle Dark Low threshold of “66”...
  • Page 26 Generally, the optimal values for these filters are set at the factory. However, some experimental approaches utilize much better postprocessing noise-reduction algorithms than can be implemented in-camera. Should you find the need to alter the filter strength, the general principle for setting the pixel noise filters is to use as little filtering as possible. Often the best way to determine this is by viewing a real-time histogram with log scaling of the frequency.
  • Page 27: Chapter 4 - Operating Features

    Chapter 4 – Operating Features Introduction This section explains the Kinetix camera system’s different modes of operation and the best modes to optimize imaging performance. Modes and Kinetix has four modes: • Dynamic Range Gain States • Sensitivity • Speed •...
  • Page 28: Regions Of Interest

    For the 16-bit Dynamic Range (DR) Mode of the Kinetix camera, the reset and readout signals propagate at a rate of 1 row per line time. However, for non-DR ports of the Kinetix camera, these signals propagate at a rate of 2 rows per line time. In 16-bit Dynamic Range Mode, the camera takes advantage of the two data channels available in the sensor to read a single row with different gains.
  • Page 29: Auto

    This rounding is only valid for the Kinetix non-Dynamic Range ports. Using the camera line times in these examples, a scan delay of 3 line times for the Kinetix 16-bit DR port yields: ��������...
  • Page 30: Scan Width

    In 16-bit Dynamic Range Mode, the Scan Width is equal to the input number. Odd and even widths are allowed. In the other ports of the Kinetix camera, which read 2 lines at a time, only even numbers of rows are used. Odd numbers of rows are incremented by 1 to the next highest even number.
  • Page 31: Device Synchronization (Triggering)

    When using this mode, the orientation of an object in an image will be the opposite of that from Down orientation. Device Kinetix offers several methods of integrating with external hardware devices. A 10-pin, Hirose HR212-10RC-10SDL(74) connector is located on the back of the camera for trigger Synchronization input/output operations.
  • Page 32: Trigger Modes

    Internal Mode Internal Mode is the default triggering mode for the Kinetix camera. The start of a sequence of acquisitions is initiated by the software/application. Once initiated, each frame captured in the sequence is controlled by the internal timing generators of the camera.
  • Page 33: Usage Summary Of Level Trigger Modes

    PVCAM command. Expose Out User-settable Expose Out Modes provide flexibility in different experimental scenarios. Modes The Expose Out trigger modes for Kinetix as of August 30, 2021, include: • First Row • All Rows •...
  • Page 34: First Row Mode

    The actions of these modes are illustrated in the next two figures (A, B). Suggested uses, subtle differences between similar modes, and additional details are described in the following text. First Row Mode The trigger signal from the camera is high only when the first row of a frame is being exposed.
  • Page 35: Line Output Mode

    Any Row Mode but using this mode does avoid frame overlap. Multiple The Kinetix camera has four independent trigger output signals. This enables hardware control over light sources that cycle through different excitation wavelengths during a Output Triggers sequence acquisition.
  • Page 36: Smart Streaming

    Control microscope, fan vibration isolation is insufficient. Kinetix solves this problem in two ways. First, a new, innovative fan mounting system was developed that isolates fan vibration from the rest of the camera. Side-by-side testing with competing products indicated that Kinetix outperforms alternatives in terms of vibration isolation.
  • Page 37: Time Stamps

    When setting the Kinetix camera on a flat surface, be careful not to block the air vents. Note: The camera has been designed to operate at 0°C. Should you need to change the temperature setpoint, contact Teledyne Photometrics Customer Service.
  • Page 38: Chapter 5 - Troubleshooting

    B) Each Kinetix comes with two identical PCIe cables and it is important that an individual cable be connected to the same port number on the camera as on the PCIe board.
  • Page 39: New Hardware Found Dialog Box Does Not Appear

    1. Make sure the new interface card is inserted in an expansion slot according to the Box Does Not computer manufacturer’s instructions. Ensure the Kinetix camera is connected and powered on at least 30 seconds Appear before starting the computer when using the PCIe interface.
  • Page 40: Pvcam Error Message Appears

    PVCAM Error If a PVCAM error message appears, note the message’s numerical code and then contact Teledyne Photometrics Customer Service. Message Appears Lengthy If you notice lengthy pauses marked by a lot of disk activity while imaging: Pauses During •...
  • Page 41: Chapter 6 - Basic Specifications

    Chapter 6 – Basic Specifications Kinetix Dimensional Drawings All measurements given in millimeters. Camera 4 lbs. (1.8 kg) Weight 58-723-004 Rev A01...
  • Page 42: Sensor Specifications

    1.80 lbs. (0.82 kg) Supply Cable Length: 4 ft. (1.22 m) Certifications: CE, UL, CUL, FCC, PSE Efficiency level VI Note: CE certification applies to the Kinetix camera only when the camera system operates with a CE-approved power supply. 58-723-004 Rev A01...
  • Page 43: Appendix A - Liquid Cooling Setup Instructions

    Appendix A – Liquid Cooling Setup Instructions Warning: Use of equipment not originally provided by Teledyne Photometrics for use with liquid cooled cameras will void any and all warranty coverage of the product. Unpack the cooler and hose assembly. Confirm the cooler and hoses are pre-filled with blue-colored coolant.
  • Page 44: Appendix B - Hotswap/Hotplug Capability

    (The “21” within the aforementioned serial number indicates the year of the build; the letter following the year-of-build–designating number indicates the month of build.) When using the latest PCIe card, Kinetix can now be turned on after the computer, and the camera can be power cycled without the need to restart the computer.
  • Page 45: Appendix C - Multi-Camera Configuration

    – Multi-Camera Configuration Using multiple Kinetix cameras with a single PCIe card: The Dolphin PXH832 PCI-Express card specified for use with Kinetix has multiple ports and can be configured for either PCI-Express 4 Lane, 8 Lane, or 16 Lane operation. By...
  • Page 46 Using long optical cables with Kinetix: The PXH832 card that comes with Kinetix is compatible with active fiberoptic PCIe cables measuring up to 100 meters in length. No special configuration of the card needs to be performed for working with these cables. Maximum achievable frame rates (in frames per second) using the fiberoptic cables are presented below.
  • Page 47 Notes 58-723-004 Rev A01...
  • Page 48 58-723-004 Rev A01...

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