Teledyne Photometrics PRIME 95B User Manual
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Teledyne Photometrics PRIME 95B User Manual

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Prime 95B
Scientific CMOS User Manual
Prime 95B Manual
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  • Page 1 Prime 95B Scientific CMOS User Manual Prime 95B Manual...
  • Page 2 Photometrics. Acrobat and Reader are registered trademarks of Adobe Systems Incorporated in the United States and/or other countries. Teledyne Photometrics and PVCAM are registered trademarks of Teledyne Technologies. Prime and Prime 95B are trademarks of Teledyne Photometrics. Intel Core is a trademark of Intel Corporation in the U.S. and/or other countries.
  • Page 3 Teledyne Photometrics warrants this product against substantial defects in materials and/or workmanship for a period of up to two (2) years after shipment. During this period, Teledyne Photometrics will repair the product or, at its sole option, repair or replace any defective part without charge to you. You must deliver the entire product to the Teledyne Photometrics factory or, at our option, to a factory-authorized service center.
  • Page 4 Teledyne Photometrics. You must notify the Teledyne Photometrics factory service center within thirty (30) days after you have taken delivery of a product or part that you believe to be defective. With the exception of customers who claim a “technical issue” with the operation of the product or part, all invoices must be paid in full in accordance with the terms of sale.
  • Page 5 In no event shall Teledyne Photometrics’ liability exceed the cost of the repair or replacement of the defective product or part.
  • Page 6 Prime 95B Scientific CMOS User Manual...

  • Page 7: Table Of Contents

    Prime 95B Scientific CMOS User Manual Table of Contents Chapter 1 - Overview About This Manual Precautions Environmental Requirement Storage Requirements Microscopes, Lenses, and Tripods Repairs Cleaning Chapter 2 - System Installation Introduction Getting to Know Prime Software Compatibility Requirements...
  • Page 8 Images Not Displayed Camera Running Too Warm PVCAM Error Message Appears Lengthy Pauses During Imaging Camera Hand with ROIs Chapter 6 - Basic Specifications Prime 95B Front, Side, Rear Views Camera Weight Power Supply Specifications Liquid Cooling Setup Instructions INDEX...

  • Page 9: Chapter 1 - Overview

    Chapter 1 - Overview About This Manual This Prime 95B Scientific CMOS Camera User Manual is divided into five chapters. Teledyne Photometrics recommends you read this entire manual before operating the camera to ensure proper use. The chapter contents are briefly described below.

  • Page 10: Environmental Requirement

    Repairs Please save the original packing materials so you can safely ship the camera to another location or return it for repairs if necessary. The Prime 95B camera system contains no user-serviceable parts. Repairs must be done by Teledyne Photometrics. Should the camera system require repairs, please contact Teledyne Photometrics Customer Service.

  • Page 11: Chapter 2 - System Installation

    Again, use only a Prime 95B PCI Express data cable and Prime PCI Express interface card with the camera. Using a di erent cable or interface card may result in in unexpected errors or permanent damage to the system.

  • Page 12: Getting To Know Prime

    Scientific CMOS User Manual Getting to Know Prime 95B Highlights of the Prime 95B camera are shown below: The Prime 95B package includes the PVCAM drivers designed to allow you to use this camera with a variety of third party imaging software - To see a list of supported software, visit the Teledyne Photometrics website.

  • Page 13: Host Computer Requirements

    CMOS camera platform provides both USB 3.0 for convenience and a PCI- Express interface to ensure that the camera is capable of sustaining the required bandwidth of the camera. The data rate of the Prime 95B camera is sustainable through both the USB 3.0 and PCI-Express interfaces.
  • Page 14 Note: The model of PCIe card shipped with the camera may di er from the one shown in the photo. Warning: Do not use the PCIe interface supplied with the QImaging optiMOS sCMOS camera with the Teledyne Photometrics Prime 95B camera. While they have a common cable and connector, they are not compatible.
  • Page 15 Prime 95B Scientific CMOS User Manual Before attempting to operate the camera, first install this interface card into the PC with the following steps: 1. Shut down the PC 2. Unplug the PC from power mains and ensure the camera is turned o 3.

  • Page 16: Connecting Prime To Pci Express Bus

    Prime 95B Scientific CMOS User Manual Connecting Prime The Prime 95B camera data cable is a quick insertion, quick release cable that to the PCIe Bus works with the interface card and camera. Either end of the cable can be plugged into either device, and in any order.

  • Page 17: Connecting Prime With Usb

    SuperSpeed+ logo and are typically blue in color. Tip: USB devices sharing the same bus as the Prime 95B contend for available bandwidth, potentially causing the camera to drop frame rate. For this reason, Teledyne Photometrics recommends isolating the camera to its own USB3.0...

  • Page 18: Chapter 3 - Theory Of Operation

    Prime 95B Scientific CMOS User Manual Chapter 3 – Theory of Operation Introduction Backside illuminated scientific CMOS (BSI Scientific CMOS) sensors are a recent development in image sensor technology. BSI CMOS sensors are able to provide the highest levels of sensitivity with a near perfect 95% quantum e ciency (QE).

  • Page 19: Gain Combining And Bit-Depth

    FPGA. The result is a single gain of approximately 1.2 e /ADU. In practice, the Prime 95B o ers both a combined ADC 16-bit output as well as a 12-bit single ADC output. The combined gain takes two measurements of a pixel for the combined ADC 16-bit output.

  • Page 20: Rolling And Global Shutter Readout

    (The row being digitized “rolls” through the sensor). For Prime 95B, the time to digitize a single row is 20µs, and consequently the delay between the start of exposure between two adjacent rows is approximately 9.6µs.

  • Page 21: Digital Binning

    Scientific CMOS User Manual Digital Binning Highlights of the Prime 95B camera are shown below: CCD image sensors are capable of charge binning (combining adjacent pixels into one super pixel). This is accomplished as part of the charge transfer process and has the advantage of increasing signal to noise in read-noise limited situations, at the expense of spatial resolution.

  • Page 22: Pixel Noise Filters

    Scientific CMOS User Manual Pixel Noise Filters Note: The Prime 95B camera ships with an optimized default setting for Real Time Pixel Noise Filtering. Normally these values do not need to be adjusted. Additionally, the features described in this section may not be controllable in the software application.

  • Page 23: Signal Processing

    Prime 95B Scientific CMOS User Manual Given the new terminology – a simplified way to visualize the region in which each filter operates is shown below: Figure 8: Pixel Noise Filter The general principle for setting the pixel noise filters is to use as little filtering as possible.

  • Page 24: Denoising

    Prime 95B Scientific CMOS User Manual Teledyne Photometrics leveraged the revolution in computational imaging with two new capabilities, PrimeEnhance™ and PrimeLocate™. Prime’s computational power matches the latest generation of GP GPU’s while making the technology easy to deploy and accessible by embedding computational power inside the camera.

  • Page 25: Localization Microscopy

    Prime 95B Scientific CMOS User Manual Localization Microscopy The hallmark feature of localization microscopy are sparse images of individual (PrimeLocate™) point emitters that blink at random times during an image sequence. By finding the centroid of each emitter’s di raction limited spot, and combining the localization results from each frame, a super-resolution image of the original fluorescence...

  • Page 26: Chapter 4 - Operating Features

    Clearing Mode Selection In normal video-rate imaging, Prime’s clearing mode should be set to “Clear Never.” The Prime 95B is continuously clearing the sensor while there are no images being acquired. This eliminates unnecessary sensor clearing and maximizes frame rates.

  • Page 27: Single And Multiple Regions Of Interest

    Prime 95B Scientific CMOS User Manual Figure 11 Tip: In some software applications, multiple clearing modes may be listed as they are required for other cameras, but when using Prime, be sure to only use “Clear Pre Sequence”. For time-lapse acquisitions with a significant delay between frames, clearing before each exposure may be necessary to clear accumulated dark current.

  • Page 28: Device Synchronization (Triggering)

    Prime 95B Scientific CMOS User Manual Prime 95B supports advanced multi-ROI acquisition in part due to the exceptionally powerful on camera FPGA. Up to 16 user defined ROIs can be collected and sent to the host PC. The regions may overlap.

  • Page 29: Trigger Modes

    Level Trigger Mode The Level trigger mode is an addition to the Prime 95B cameras that enables hardware control over the exposure time of each frame. A TTL pulse can be used as input to the camera and the length of time that the pulse stays high will equal the length of time that the camera acquires data for that image.

  • Page 30: Expose Out Behaviors

    Ideally, the same system controlling the exposure time for the camera is also managing the illumination cycles to ensure data integrity and eliminating image cross-talk. The expose-out features of the Prime 95B o er some system flexibility in this regard, as they can provide output trigger signals for illumination control through the di erent expose out modes.
  • Page 31 Prime 95B Scientific CMOS User Manual Although “First Row” behavior provides the maximum camera frame rates, it does not avoid the overlap due to rolling shutter. This mode is not recommended if trying to alternate between excitation wavelengths. Figure 16 Any Row The “Expose Out”...
  • Page 32 Prime 95B Scientific CMOS User Manual Figure 18 All Rows provides an e ective global shutter with the speed and low noise benefits of rolling shutter, but eliminates rolling shutter motion artifacts. This mode is recommended for synchronizing the camera with high speed light sources that alternate excitation wavelengths for high speed multi-channel fluorescence.
  • Page 33 Prime 95B Scientific CMOS User Manual When Exposure time is more than readout time: Figure 20 Below is a comparison of the di erent Expose Out behaviors under di erent exposure time lengths: Figure 21 From this comparison, the following conditions can be defined for each expose...

  • Page 34: Multiple Output Triggers

    Prime 95B Scientific CMOS User Manual Multiple Output Triggers The Prime 95B camera has four independent trigger output signals. This enables hardware control over light sources that cycle through di erent excitation wavelengths during a sequence acquisition. For example, a multi-wavelength LED light source can rapidly switch between two di erent fluorochromes.
  • Page 35 Prime 95B Scientific CMOS User Manual The following table shows the frame rate advantages possible using multiple trigger outputs and multiple triggers with SMART Streaming™ enabled. Fans are often used to remove heat from the camera due to their convenience.

  • Page 36: Advanced Features

    + 1. The smallest ROI is a 3x3 pixel region (radius=1), and the largest ROI is a 31x31 pixel region (radius=15). If it is required to go outside of these ranges, please contact Teledyne Photometrics customer service. These ranges were chosen based on the specific use case of...

  • Page 37: Time Stamps

    Scientific CMOS User Manual Time Stamps Prime 95B is able to output “metadata” that describes data associated with each frame. Metadata contains useful information like the exposure time, ROI location and time stamps. Metadata is inserted directly into the frame bu er and transferred together with the image data.

  • Page 38: Chapter 5 - Troubleshooting

    1. Make sure the new interface card is inserted in an expansion slot according to the computer manufacturer’s instructions 2. Ensure Prime 95B is connected and powered on at least 10 seconds before starting the computer when using the PCIe interface.

  • Page 39: Images Not Displayed

    If no images appear: 1. Confirm the camera switch is set to on. 2. Confirm that the Prime 95B camera is selected in the imaging software application. 3. Power o the camera and the host computer and check all system connections (particularly the DATA and power cables), then restart.

  • Page 40: Chapter 6 - Basic Specifications

    Prime 95B Scientific CMOS User Manual Chapter 6 – Basic Specifications Prime 95B: Front, Side And Rear Views Figure 24: Prime 95B Front View Prime 95B Side View Prime 95B Rear View Camera Weight Weight: 3.8 lbs. (1.7 kg) Sensor Specifications...

  • Page 41: Power Supply Specifications

    Prime 95B Scientific CMOS User Manual Power Supply Specifications Voltage Input: 100-240 V~ @ 50-60 Hz Current Input: 2.5 A (110V nominal) Voltage Output: +12V @ 8 A Maximum Power Output: 140 W Power Supply Weight: 1.80 lb (0.82 kg) Supply Cable Length: 4 ft.

  • Page 42: Liquid Cooling Setup Instructions

    Scientific CMOS User Manual Appendix Liquid Cooling Setup Instructions Warning: Use of equipment not originally provided by Teledyne Photometrics for use with liquid cooled cameras will void any and all warranty coverage of the product. 1. Unpack the cooler and hose assembly.

  • Page 43: Index

    Prime 95B Scientific CMOS User Manual Index Advanced Features, 28 Operating Frequencies, 18 Bias O set, 13, 18 PCIe Connection, 3, 5-9, 30 Binning, 13 Power Connector, 8 Power Supply, 2-4, 8, 33 Power Switch, 4, 8 Calibration, 11 Precautions, 1, 3...
  • Page 44 Prime 95B Scientific CMOS User Manual www.photometrics.com Main Phone: +1 520.889.9933 Support: +1 604.530.5800 / +1 800.874.9789...

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