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3DHISTECH PANNORAMIC MIDI RX 1.0 User Manual

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PANNORAMIC® MIDI RX
1.0
U
'
G
SER
S
UIDE
May 13, 2021 – Rev.1
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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  • Page 1 PANNORAMIC® MIDI RX ’ UIDE May 13, 2021 – Rev.1 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

  • Page 2: Table Of Contents

    PANNORAMIC® MIDI RX 1.0 CONTENTS Contents Contents Disclaimer Declaration of Conformity About this User's Guide Character Formats and Symbols Notes Regarding Operational Safety Notes on Warranty Terms and Abbreviations Product Description 1.1 Product Overview 1.1.1 Intended Use 1.1.2 Features and Benefits 1.1.3 Warning and Information Labels 1.1.4...
  • Page 3 PANNORAMIC® MIDI RX 1.0 CONTENTS 4.1.2 Exiting Pannoramic® Scanner Software for Research control software Main Window Functions Hotkeys Scanning Modes and Settings Brightfield Scanning Mode 5.1.1 Profile Settings Panel 5.1.2 Microscope Control Panel 5.1.3 Quick Preview Functions 5.1.4 Quick Microscope Functions Fluorescence Microscope Mode 5.2.1 Profile Settings Panel 5.2.2 Microscope Control Panel...
  • Page 4 PANNORAMIC® MIDI RX 1.0 CONTENTS 9.3.3 Preventive Maintenance Troubleshooting 9.4.1 Fluorescent Applications (Optional) Maintenance 9.5.1 Opening the Protective Covers 9.5.2 Removing a Slide 9.5.3 Installing and Removing the Filter Module (Fluorescent Option) 9.5.4 Transporting Pannoramic® MIDI RX Technical Data MAY 13, 2021 FOR RESEARCH USE ONLY.

  • Page 5: Disclaimer

    IP are the sole properties of the 3DHISTECH Ltd. 3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequential damage or incidental damage,...
  • Page 6 RF sources), as these may interfere with the proper operation. Third-party disclaimer 3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general or specific damage, indirect damage, consequential damage or incidental damage, including...

  • Page 7: Declaration Of Conformity

    DECLARATION OF CONFORMITY Declaration of Conformity 3DHISTECH Ltd. declares that the product Pannoramic® MIDI RX slide scanner is designed and produced with consideration of specified requirements according to the IEC 61326-2-6:2012 standard, and conforms to the directives and other standards listed below: IEC 61010-1:2010, “Safety Requirements for Electrical Equipment for Measurement, Control, and...

  • Page 8: About This User's Guide

    This User's Guide is a part of the Pannoramic® MIDI RX system identified by the relevant article numbers included in the 3DHISTECH Ordering Guide. MAY 13, 2021 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

  • Page 9: Character Formats And Symbols

    PANNORAMIC® MIDI RX 1.0 CHARACTER FORMATS AND SYMBOLS Character Formats and Symbols The following character formats and symbols call your attention in this manual: Example (text in bold) Words or characters that appear on the screen. These include field names, screen and window titles, push-button and menu names, paths or options.

  • Page 10: Notes Regarding Operational Safety

    PANNORAMIC® MIDI RX 1.0 NOTES REGARDING OPERATIONAL SAFETY Notes Regarding Operational Safety The below section of this User’s Guide contains information and warnings of a kind that must be followed by owner/operator personnel. Warning and advisory symbols which are used on the base unit of the Pannoramic® MIDI RX and in section 1.1.3 Warning and Information Labels have the following meanings: Warning! Disconnect mains plug! Remember to unplug the power cord before you open the...
  • Page 11 PANNORAMIC® MIDI RX 1.0 NOTES REGARDING OPERATIONAL SAFETY Warning! Never operate the device in explosion-risk environment. Warning! If protective devices are found to be ineffective, the Pannoramic® MIDI RX must be shut down and precautions must be taken to prevent unintended usage. For necessary repair work or work to restore proper operating condition, you should contact the 3DHISTEC H Customer Service.
  • Page 12 PANNORAMIC® MIDI RX 1.0 NOTES REGARDING OPERATIONAL SAFETY Warning! Use of this instrument in a dry environment, especially if synthetic material is present (synthetic clothing, carpets, etc.) may cause damaging electrostatic discharges that may cause erroneous results. Warning! If a Lumencor SOLA fluorescent illumination unit is used, the corresponding warnings included in the manufacturer’s operating manual have to be observed.
  • Page 13 PANNORAMIC® MIDI RX 1.0 NOTES REGARDING OPERATIONAL SAFETY Caution! Dirt or dust may adversely impact the operating capability of the Pannoramic® MIDI RX. For this reason, measures must be taken to eliminate such influences on a maximum possible degree. During periods of non-use, the Pannoramic® MIDI RX must be protected with a dust cover.

  • Page 14: Notes On Warranty

    NOTES ON WARRANTY Notes on Warranty 3DHISTECH Ltd. – as the Product Manufacturer – warrants the Pannoramic® MIDI RX to be free from faults in material and workmanship at the moment of installation. Defects must be notified immediately on identification and maximum efforts must be undertaken to minimize damage. On...

  • Page 15: Terms And Abbreviations

    PANNORAMIC® MIDI RX 1.0 TERMS AND ABBREVIATIONS Terms and Abbreviations Term Description Comma Separated Value Field of View Numerical Aperture A phenomenon that leads to a dramatic loss of fluorescence emission intensity (a Photobleaching fluorophore permanently loses the ability to fluoresce) due to photon-induced chemical damage and covalent modification, therefore fluorescent signals cannot be properly detected.

  • Page 16: Product Description

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION 1 Product Description 1.1 Product Overview 1.1.1 Intended Use The Pannoramic® MIDI RX is designed to digitize biological samples and specimens (histological sections, typically) using transmitted light microscopy of fluorescent microscopy techniques. Warning! After each scanning make sure to inspect the digital slide for over-exposed areas. If the slide has been scanned based on improperly set parameters, it can highly affect the result because of information loss.
  • Page 17 PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION Key features of the Pannoramic® MIDI RX scanner are: • A single monochrome camera is used both for Brightfield and Fluorescent scanning . • LED-based RGB illumination unit • Up to 9 freely selectable physical color filters for fluorescence •...

  • Page 18: Warning And Information Labels

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION 1.1.3 Warning and Information Labels The following figure contains the labels used on the Pannoramic® MIDI RX main unit, the external mains power supply unit, and the Lumencor SOLA illumination unit. Figure 1: Warning and information labels MAY 13, 2021 FOR RESEARCH USE ONLY.

  • Page 19: Base Unit

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION 1.1.4 Base Unit The following figure shows the main parts of the base unit. Figure 2: Main parts of the base unit Protective covers Point Grey Grasshopper 3 or pco.edge 4.2 bi camera Fluorescent option A Lumencor SOLA fiber-coupled illumination unit with Lumencor illumination adapter and cable Fluorescent option B Lumencor SPECTRA 6 Light Engine fiber-coupled illumination unit...

  • Page 20: System Overview

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION 1.2 System Overview The standard scope of delivery of the product includes the following hardware and software items. 1.2.1 Pannoramic® MIDI RX system The following figure shows the main components of the Pannoramic® MIDI RX system. Figure 3: Pannoramic®...

  • Page 21: Hardware Options

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION 1.2.2 Hardware Options Notice: The configuration of the control computer and monitor can vary depending on the product order. Cameras Point Grey Grasshopper 3 1 CCD monochrome camera for high-quality applications (available for “Brightfield only” MIDI RX systems) pco.edge 4.2 bi Back illuminated 16-bit sCMOS pco.edge 4.2 bi camera for Fluorescent application...
  • Page 22 Notice: If Lumencor SPECTRA 6 unit is ordered, the custom light source and filter modules can be variable. For more information, contact 3DHISTECH customer service. Objectives • Plan-Apochromat 10×/NA 0.45 (optional, can be used only for FL scanning) •...

  • Page 23: Control Software

    PANNORAMIC® MIDI RX 1.0 PRODUCT DESCRIPTION Fluorescent Option for Pannoramic® MIDI RX Fluorescent illumination unit Description Lumencor SOLA Fluorescent illumination unit Lumencor SPECTRA 6 Light Engine illumination unit 1.2.3 Control Software • Pannoramic® Scanner Software for Research 1.0 (control software) Optional: •...

  • Page 24: Installation

    PANNORAMIC® MIDI RX 1.0 INSTALLATION 2 Installation Warning! The installation of Pannoramic® MIDI RX must be carried out by a trained professional! In any other case the warranty will be automatically voided. The supplier or distributor is not liable for any damages caused by an installation carried out by an unauthorized person. Only qualified service assistants are allowed to install the hardware and software.
  • Page 25 C:\Program Files\3DHISTECH • C:\ProgramData\3DHISTECH • C:\Program Files (x86)\3DHISTECH • (E:\ScannerSwap\ as the SWAP folder by default) We recommend that you use the "SWAP" partition for storing temporary files generated during scanning, as the lack of space required will result in interruption of scanning.

  • Page 26: Control Computer Ports

    PANNORAMIC® MIDI RX 1.0 INSTALLATION 2.2 Control Computer Ports The following figure shows the location of the connectors to enable easier transportation of the product for a short distance, by disconnecting cables and detaching hardware components from each other. Figure 4: Ports on control computer Status display DVD drive (RW) Power on/off button...

  • Page 27: Connectors And Cables

    PANNORAMIC® MIDI RX 1.0 INSTALLATION 2.3 Connectors and Cables • Control – USB (between scanner and control computer) • Preview camera – USB cable (between scanner and control computer) • Point Grey Grasshopper 3 – USB 3.0 cable (between Point Grey Grasshopper 3 camera and control computer) •...

  • Page 28: Coupling The Lumencor Spectra 6 Unit With The Scanner

    PANNORAMIC® MIDI RX 1.0 INSTALLATION 2.5 Coupling the Lumencor Spectra 6 Unit with the Scanner To operate the Lumencor Spectra 6 Light Engine fluorescent illumination unit, it has to be attached to the Pannoramic® MIDI RX base unit, and connected to the camera, and the control computer with cables.

  • Page 29: External Power Supply Unit

    PANNORAMIC® MIDI RX 1.0 INSTALLATION 2.6 External Power Supply Unit For the proper operation of the scanner, the SINPRO – MPU101-108 24V external power supply unit must be connected between the mains power and the device. Front panel: main power switch power supply connector Back panel: detachable power cord to connect the external power supply unit with the main...

  • Page 30: Preparing Slides And Trays

    PANNORAMIC® MIDI RX 1.0 PREPARING SLIDES AND TRAYS 3 Preparing Slides and Trays This section demonstrates how to prepare slides and trays. You may use slides and coverslips that meet the following specifications: Slides Cover slips Length 75.0 to 76.0 mm max.
  • Page 31 PANNORAMIC® MIDI RX 1.0 PREPARING SLIDES AND TRAYS Warning! If the scanner is equipped with a 40× objective, the outer rim of the sample should be at least 3mm distance from the slide edge on the left side, since the working distance of the 40×...

  • Page 32: Affixing Barcode Stickers To Slides

    PANNORAMIC® MIDI RX 1.0 PREPARING SLIDES AND TRAYS 3.1 Affixing Barcode Stickers to Slides This section describes how to affix barcode stickers to large slides. Barcodes enable virtual slide identification. Figure 7: slide with a barcode sticker The label area (1) is recorded by the preview camera and can be displayed by the Pannoramic®...

  • Page 33: Inserting And Removing Slides

    PANNORAMIC® MIDI RX 1.0 PREPARING SLIDES AND TRAYS 3.2 Inserting and Removing Slides To insert slides into a tray Figure 8: Inserting slides into a tray A tray can contain a maximum of 12 slides. • Push a slide (1) into the desired slot (4) of the tray (3) with its barcode end (2) first. •...

  • Page 34: Inserting And Removing The Tray

    The edge of the broken area of the slide is sharp and its removal can lead to injury and contagion! Broken slide can be removed only by a well-trained person! Only original 3DHISTECH trays are allowed for use with Pannoramic® MIDI RX! Notice: The cover slip and the label area should be on the top.
  • Page 35 PANNORAMIC® MIDI RX 1.0 PREPARING SLIDES AND TRAYS Caution! To insert or remove a filled tray from the device, first check tray for incorrectly inserted slides (refer to Figure 9) and always ensure also when moving the tray, slightly tilt it to its closed side to prevent the slides from accidentally falling out.

  • Page 36: Software Description

    PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION 4 Software Description 4.1 Starting/Exiting Pannoramic® Scanner Software for Research 4.1.1 Starting the Control Software To start the program, double-click on the program icon on the desktop. Before software initialization the user interface is opened, and the mode selection icons are displayed.

  • Page 37: Main Window Functions

    PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION 4.2 Main Window Functions Figure 13: Pannoramic® Scanner Software for Research 1.0 main window The following functions are available for you to select from at the main menu bar: Switch between Microscope and Planner modes Microscope initialization –...
  • Page 38 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Objective changer Live view is on Live view is on with the shutter closed Toolbar for live images Click to display the toolbar for Live view. These functions are only available for the displayed live image (i.e., Live view is ON), but not available for camera images during digitization.
  • Page 39 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION • – Image intensity Figure 15: Image intensity The intensity of the pixel at which the cursor is placed is displayed by I in the Image intensity window. X and Y stand for the location of the pixel within the image coordinate system. •...
  • Page 40 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Scan Properties Unique settings can be defined even for each separate scanning. These settings are included but not editable on the profile cards in Planner mode. • Scan to Local Figure 16: Scan to Local Slide naming Slide name can be defined manually or automatically.
  • Page 41 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Click at the left of a list item to open the Barcode editor window in which the settings of the selected profile can be edited. Figure 18: Barcode editor window If you click Add new item ... on the list, blank input fields will be displayed. Figure 19: Create new barcode •...
  • Page 42 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION • If the option Separator is selected at Parsing modes, the character used as a separator in the selected barcode type can be defined in the Separator input field. Figure 20: Parsing settings Click to delete range values set for the corresponding field.
  • Page 43 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION • Target format: The slide is converted to the selected final format. Click one of the buttons to define the final slide format (MRXS, Tiled-TIFF, Roche-TIFF, SVS, DICOM). • Click to open MRXS, Tiled-TIFF and DICOM conversion settings panel: MRXS settings Figure 21: MRXS settings ▪...
  • Page 44 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION ▪ Automatic: the converter automatically selects the most appropriate method based on the size of the source slide. ▪ Force simple tiff: TIFF files use 32-bit offset addressing; therefore, file size is limited to a maximum of 4Gb. ▪...
  • Page 45 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION ▪ Shared plane position (active by default) ▪ Shared optical reference (active by default) Conversion save path: The folder set in this box is designed to store the final slides after conversion. Conversion progress is displayed in the Converter queue window of the converter.
  • Page 46 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Click to assign the appropriate Quant probe to the digital slide. If the scanning of slide has finished, the progress of Quant evaluation is displayed in the QuantCenter™ Processing Queue window. For further information on QuantCenter™, please read QuantCenter™ User's Guide. Other settings •...
  • Page 47 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Scan to Server Figure 25: Scan to Server settings • Server settings Select the dedicated server to which you want to scan from the Server drop-down list. Click the arrow to show the list of saved server profiles, or click Add new item ... at the bottom of the list to create another server connection.
  • Page 48 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Figure 26: Window of server editor Server Editor stores each of the created server in a list. List entries can be removed, or a new entry can be added if required. With the Filters function you can narrow down the results by searching for a specific server data.
  • Page 49 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION • Slide naming Barcode parsing: Similar to the settings of the Scan to local panel, barcode settings for parsing can be modified in the Barcode Editor window. Figure 28: Barcode Editor If using MIL for barcode reading, you can select the following barcode types: 1D barcode types •...
  • Page 50 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION 2D barcode types • Aztec • Data Matrix • PDF417/Compact • Micro PDF417 • • Micro QR • MaxiCode Notice: If embedded barcode library (provided by Vintasoft barcode reader software) is used, refer to Figure 70 Barcode settings in chapter 6.2 Service for the list of supported barcode types.
  • Page 51 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Quant settings • Run QuantCenter™: Turn this function on for automatic evaluation of the completed digital slide by using the integrated QuantCenter™, right after the scanning has finished. Other settings • Scan separately: The FOV areas of the preview image will be scanned and saved separately as discrete digital slides.
  • Page 52 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION Each preview of slides in the tray is taken automatically. Preview types are the following (from top to bottom): • Brightfield • Pre-Scan • Pre-Scan High-Resolution • Pre-Scan + High-Resolution Activate Retake Preview for all the slides in the magazine if necessary. This operation can be initiated from the slide preview window as well.
  • Page 53 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION The Profile Browser window containing a list of previously saved profiles is opened. Based on the Illumination type, profiles are grouped into two clusters: Brightfield and Fluorescence. Additional profiles can be created here as well, and you can also search for a specific profile by filtering based on the following data: •...

  • Page 54: Hotkeys

    PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION – Help In the About window the version of the Pannoramic® Scanner Software for Research control software and licensing details are included. Figure 31: About window 4.3 Hotkeys • Press F1 to open/close hotkeys window •...
  • Page 55 PANNORAMIC® MIDI RX 1.0 SOFTWARE DESCRIPTION • SHIFT+mousewheel: Change Focus by step +36/-36 • W/S: Change Focus by step +1/-1 (fine-tuning) • Arrows: Move stage (within a limited area) • F2: Change Pseudocolor • CTRL+F: Start Autofocus • Left Mouse Button+Drag: Pan view •...

  • Page 56: Scanning Modes And Settings

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5 Scanning Modes and Settings After microscope initialization, if fluorescence hardware option is provided, you can select from the following two modes: • Brightfield scanning mode (See chapter 5.1 Brightfield Scanning Mode for more information.) •...

  • Page 57: Profile Settings Panel

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.1.1 Profile Settings Panel Objective settings This field displays the objective used for scanning. Objective type: 20x; 40x. Figure 33: Objective settings Focus settings for the scan profile Figure 34: Focus settings for scan profile •...
  • Page 58 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Sample detection This panel offers options for threshold setting and selection of the area to be scanned. Figure 36: Sample detection window • Source: Brightfield preview – a normal type of illumination in the microscope for scanning the slide.
  • Page 59 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Use marker with threshold If the specimen cannot be identified as required for digitization, (for example, weak contrast), it can help to encircle completely the specimen with a black marker pen. You have to draw a full circle with the pen to enable the detection. Marker pen detection will make specimen detection easier by limiting the scanned area.
  • Page 60 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Threshold enhancement Remove coverslip – If selected, the edges of the coverslip are not detected as specimen to be scanned. Depending on the threshold level, it is possible that the edges of the coverslip are detected as areas to be digitized. Figure 38: Preview image with and without coverslip edge Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to...
  • Page 61 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Scanmap A previously saved scanmap can be loaded and applied. • Use Threshold: Preview settings defined in the Microscope Controller Panel / Preview window are applied as the basis of scanmap. • Load scanmap: This function is useful if the slides contain areas of interest that cannot be detected by the preview camera.
  • Page 62 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Z-Stack: For Extended focus and Z-Stack scanning methods, you can determine the desired number of focus levels with the Levels selection box. The current focus value is the initial level. If you set 5 for example, then the initial level, plus four (two focus levels above and below the FOVs) are captured.
  • Page 63 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Image settings You can choose the following predefined image formats (and a subsequent custom option) from the Image Quality drop-down list in the Image Encoding section of the window: • Excellent (JPEG: 90) •...

  • Page 64: Microscope Control Panel

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.1.2 Microscope Control Panel The Microscope Control panel includes the main control features of the microscope. 5.1.2.1 Preview Window The Preview panel offers options for threshold setting and selection of the area to be scanned.
  • Page 65 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Preview Type setting determines the type of illumination. In Brightfield mode when using MIDI RX, the following preview types are available: • Brightfield preview – a normal type of illumination in the microscope for scanning the slide.
  • Page 66 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Notice: If a hematology slide is detected including blood smear, the Remove Coverslip function cannot be activated and focusing on the coverslip area is not available. If you want to use the Remove Coverslip function, first switch to Custom Scanmap. Fill holes inside scanmap –...
  • Page 67 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Use marker with threshold – If the specimen cannot be identified as required for digitization, (for example, weak contrast), it can help to encircle completely the specimen with a black marker pen. You have to draw a full circle with the pen to enable the detection.
  • Page 68 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Preview Toolbar This section is located at the bottom of the preview image. • Save preview – Click this icon to save the image of the slide recorded with the preview camera. •...
  • Page 69 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Edit – The size of selected area (positive or negative) can be modified at the borderlines or corners. Figure 46: Edited negative and positive selection masks • Crop – Crop the area of the selected area. •...
  • Page 70 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • TMA tool – TMA tool is used for selecting TMA spots on TMA slides. You can set the selection settings on the following four tabs: Figure 47: TMA grid preview • TMA Grid Setting –...
  • Page 71 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Use selection box restriction – The preview image shows the area that will be digitized with a red and/or an orange mask. If the Use selection box is enabled, and the crop function is selected, a black frame displayed in the image, and only the area inside the box is scanned.
  • Page 72 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Areas that will be scanned These areas are digitized based on user settings (for example, Fill holes in scan area) and not detected by the selected threshold value. Detected sample areas that will be scanned These areas are digitized.
  • Page 73 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Click the button to display barcode label area. To rotate the label area by 180°, click Figure 51: Barcode label area window – Undo / Redo modification made during specifying the Scanmap. These buttons are inactive when Use threshold function is applied.
  • Page 74 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.1.2.3 Exposure Indicator Activate Exposure indicator function by clicking . Click to calculate exposure time for the actual Live image. In Brightfield mode there is no option for manually setting the values. Exposure time can be displayed in microsecond, millisecond, and in second. Figure 53: Window of Exposure Indicator in Brightfield scan mode Image Intensity Click...

  • Page 75: Quick Preview Functions

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.1.3 Quick Preview Functions Use Ctrl+mouse wheel to zoom in or out to the preview area or the Live image. A quick preview function tool pops up by right-clicking to the preview area. It allows a quick access to main preview-related functions: •...
  • Page 76 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Autofocus Figure 58: Autofocus • Live tools Figure 59: Live tools Fast mode Screenshot Magnifier Ruler Image processor plugins Add scanmap • Auto exposure Figure 60: Auto exposure MAY 13, 2021 FOR RESEARCH USE ONLY.

  • Page 77: Fluorescence Microscope Mode

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.2 Fluorescence Microscope Mode Fluorescence Microscope View consist of three different sections: • Profile Settings panel: Profile settings containing parameters used for scanning • Microscope Control Panel: Live microscope movement functions • Main functions: Located at below the window header, these functions are identical in both modes.
  • Page 78 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • E / Z: Multilayer option: Select Extended focus or Z-stack for scanning the required channels in accordance with the scanning mode set in the profile. • D: Deconvolution can be activated per channel in this column. Figure 61: Filter table Objective Settings This field displays the objective used for scanning.
  • Page 79 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS If you deselect the Use fine focus checkbox, the software immediately starts scanning based on the interpolated values, without pre-fine-focusing. Figure 62: Focus settings • Auto focus: Focus value is set automatically to match sample characteristics. •...
  • Page 80 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Pre-scan high-resolution – creates a High-Resolution fast FL preview image of every FOV within the selected area (in single layer DAPI, with an illumination time of 20ms) that is displayed in the Profile Wizard window. Scanning process takes a couple of minutes only and cannot be interrupted.
  • Page 81 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Everything within marker To scan everything within the marked area, check the Everything within marker selection box. Alternatively, click in the preview window to activate this function. Figure 63: Scan everything within the marked area (selected / not selected) •...
  • Page 82 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to digitize coverslip edges. Select Remove coverslip function to eliminate those areas. Warning! Do not use Remove coverslip function on TMA slides! The layout of TMA slides may mislead the detecting algorithm.
  • Page 83 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Loaded scanmap: This function is useful if the slides contain areas of interest that cannot be detected by the preview camera. This function along with saving profiles helps to scan all slides with the most adequate settings. To load a mask, click first, then browse for the scanmap to be loaded.
  • Page 84 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Figure 67: Focus level and step size Scan settings Figure 68: Scan settings • Change filters after whole slide scanning To optimize speed, filter change should take place after the digitization of the slide with one channel.
  • Page 85 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Caution! If the specimen area is barely visible, it is possible that the Pannoramic® Scanner Software for Research control software will analyze that area as an empty one. • Maximum exposure time – A limit value can be set to determine that the exposure time cannot be longer than the time of focusing and calculation of exposure.
  • Page 86 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Image settings Choose one of the following predefined image formats (and a subsequent custom option) from the Image Quality drop-down list: • Excellent (JPEG: 90) • Good (JPEG: 80) • Medium (JPEG: 60) •...

  • Page 87: Microscope Control Panel

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.2.2 Microscope Control Panel 5.2.2.1 Preview Window Click to open the Preview panel that offers options for threshold setting and selection of the area to be scanned. Figure 71: Pre-scan preview image Threshold options This section is located at right side of the preview window.
  • Page 88 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Preview Types In FL Microscope mode the following additional preview modes are available: Brightfield preview – a normal type of illumination for scanning. This option is recommended when scanning in Brightfield mode. Pre-scan –...
  • Page 89 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Caution! It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to digitize coverslip edges. Select Remove coverslip function to eliminate those areas. Warning! Do not use Remove coverslip function on TMA slides! The layout of TMA slides may mislead the detecting algorithm.
  • Page 90 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Manual threshold Set value manually or calculate value by using the Calculate Threshold function (can be activated only when manual thresholding is selected). This sets the sensitivity of the specimen detection. To set the desired threshold value manually, change the setting in the Specimen threshold level selection box in the range of 0-255.
  • Page 91 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Dilate mask Adjust the slider to enlarge or decrease the scanned area. To switch to manual mode, click the top left corner of the button and the required value can be entered in the box Preview Toolbar This section is located at the bottom of the preview image.
  • Page 92 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Edit The size of selected area (positive or negative) can be modified at the borderlines or corners. Figure 75: Edited negative and positive selection masks • Crop – Crop the area of the selected area. •...
  • Page 93 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • – Rectangle tool Draw rectangle shaped area. • – Ellipse tool Draw ellipse selection. • – Polygon tool Area selection by defining a polygon shape. Finish area selection by double-clicking with the mouse at or near the starting point. •...
  • Page 94 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • – Clear scanmap The entire scanmap area will be cleared and reset to default. • – Use selection box restriction The preview image shows the area that will be digitized with a red and/or an orange mask. If the Use selection box is enabled, and the crop function is selected, a black frame displayed in the image, and only the area inside the box is scanned.
  • Page 95 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Areas that will be scanned These areas are digitized based on user settings (for example, Fill holes in scan area) and not detected by the selected threshold value. Detected sample areas that will be scanned These areas are digitized.
  • Page 96 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Figure 80: Pseudo-colorized pre-scan image Click the button to display barcode label area. To rotate the label area by 180°, click Figure 81: Barcode label area window – Undo / Redo modification made during specifying the Scanmap. These buttons are inactive when Use threshold function is applied.
  • Page 97 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Set the correct focus value of the desired preview position by clicking SetI1, SetI2, or SetI3. If you want to go back to any of the previously set focus points, click the appropriate button.
  • Page 98 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Exposure Settings You can set the Exposure time weight (%) here (displayed under TW in the Channel Settings frame). Set Lumencor excitation intensity at the Excitation intensity (%) field. The defined value will be used as maximum when applying Auto Exposition time if you activate the Max. excit.

  • Page 99: Quick Preview Functions

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.2.3 Quick Preview Functions Use Ctrl + mouse wheel to zoom in or out to the preview area or the Live image. 5.2.3.1 Quick Toolbar of Preview Window Figure 86: Settings of preview quick toolbar A Quick Toolbar pops up by right clicking any of the control menu items.
  • Page 100 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.2.3.2 Quick Microscope Functions A quick microscope functions tool can be displayed upon right-clicking on the background of the main window (outside the preview window). It includes quick-access-buttons to main functions for MIDI RX: Figure 87: Quick toolset for microscope •...
  • Page 101 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • Microscope mode Brightfield Fluorescence Figure 91: Microscope mode • Live tools Fast mode Screenshot Magnifier Ruler Image processor plugins Use pseudo-color Add scanmap Figure 92: Live tools • Auto-exposure Figure 93: Auto-exposure MAY 13, 2021 FOR RESEARCH USE ONLY.

  • Page 102: Bottom Menu Bar Of The Main Window

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.2.4 Bottom Menu Bar of the Main Window Profile History This function can be activated by clicking in Preferences. During setting scanning profile all the previously used parameters will be saved automatically. Each modification in the profile creates a so-called Milestone unit.

  • Page 103: Planner Mode

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.3 Planner Mode The whole scanning procedure can be planned in this mode. Assign profiles to slides, modify scan-maps without performing physical loading of slides. In Planner mode not only the scanning of the actually loaded tray can be planned, but also profile assignment to slides of prepared virtual trays is available to be performed.
  • Page 104 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Take all previews – Preview images of all the slides on the tray are taken automatically. Select the required preview type from the following (from top to bottom): Brightfield, Pre-scan, Pre-scan high-resolution, Pre-scan + high-resolution. Figure 97: Take all preview selector Load / Save Plan menu –...
  • Page 105 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Profile management for all the magazines – The following profile-related tasks applicable for the planner can be performed: • Add current profile to all racks • Open profile for all racks • Detach current profile from all racks •...

  • Page 106: General Functions At The Tray Menu

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.3.2 General Functions at the Tray Menu Rack view mode selector Switch view by clicking this button. Click to add a virtual tray to the planner, and remove a virtual tray from the planner. Figure 99: Rack view mode selector Profile management for selected magazine menu includes the following global settings applicable to all trays:...
  • Page 107 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS • You can also set Scan properties range to a slide with a profile attached. When more profiles are attached to a slide, always the last scan record will be set with the selected scan property.

  • Page 108: Slide View With Card

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.3.4 Slide View with Card Select from Preferences / Appearance settings / Planner layout selector to activate this view. Depending on the actual view preference selected at , slide positions can have a horizontal or vertical layout.
  • Page 109 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Figure 104: Vertical Slide view with card By clicking profiles can be added or removed for/from the slide, respectively. The parameters of the assigned profile are displayed in a card. The colors of a Brightfield and a Fluorescence profile cards are different.
  • Page 110 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS The following information are included in a profile card: • Profile name in the top left corner. • Profile status is marked with spots of different color: – Stored – Edited • Scanning mode icon representing Brightfield or Fluorescence mode •...
  • Page 111 PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.3.5 Table View Mode By clicking each slide will be displayed in a separate row. Click to assign profile to a slide, and to detach a profile from a slide. A slide can have more than one profile assigned to it, and these profiles can either be BF or FL if the scanner is capable to operate in these two scanning modes.

  • Page 112: Card Collection View Mode

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS 5.3.6 Card Collection View Mode If you click the button, each of the slide previews are displayed in the window. Add profiles by clicking and detach profiles by clicking . Functions are the same as with the other modes.

  • Page 113: Progress Features

    PANNORAMIC® MIDI RX 1.0 SCANNING MODES AND SETTINGS Each of the assigned profiles are checkable and modifiable. The panel at the right side includes all the profile settings, in coherence with the view type of the Profile Setting panel. Slide preview is displayed in the Preview window in which the scanmap can be modified until beginning the scanning process.

  • Page 114: Settings And Services

    PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES 6 Settings and Services Activate Settings by clicking and Services by clicking at the right side of the main menu bar. 6.1 Settings Activate Settings by clicking at the right side of the main menu bar. •...
  • Page 115 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES This panel contains user settings that have global effect on the GUI and some of its functions. Appearance settings Planner layout editor ▪ Slides with card ▪ Table ▪ Card Collection Initial Preview window position ▪...
  • Page 116 Scanning History Hardware log Scanner hardware operations are contained in this log. To enable this function, first the database from the following folder must be loaded: C:\ProgramData\3DHISTECH\SlideScanner\HWLogDataBase. The following motor movements are recorded in the log list: ▪ Camera Changer ▪...
  • Page 117 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES Software log All the circumstances and states of the slide scanning procedure are saved. LogID is the sequence number of scanning performed on the scanner in use (Profile Name, Illumination mode, Scan type, Scan Start, Scan Finished, URL, Error). Figure 111: Scanning log Filtering for the following parameters is available: ▪...
  • Page 118 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – Basic settings Figure 112: Basic settings On this panel, you can define the swap file location in the Swap location field, set the value of Shutter auto close time in FL, and define a value in the Number of compensation images the preview camera should take, if needed.
  • Page 119 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – Profile behavior settings Figure 113: Profile behavior settings Use profile history save – With this function each profile modification is saved in Profile History from which the different stages can be reloaded. Save interval –...
  • Page 120 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – External application call Figure 115: External application call window Two of the three settings are responsible for opening external applications (.exe or .bat) as defined (for example, SlideViewer or CaseViewer™ software can be opened after scanning is finished).
  • Page 121 You can test whether the Scanning Error Occurred settings work by clicking Caution! If C:\ProgramData\3DHISTECH\SlideScanner\MessengerUserPreferences.xml is missing or damaged, the External application call feature will not be able to read the address book, therefore it will not operate. To solve this issue, the address book must be repaired or created again.
  • Page 122 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES In the Message Call section of the window complex messaging functions can be defined. Here you can setup parameters of the mail server, add or remove users, and set the message body in the Message subject field by entering a text for example, to inform recipients on the location of the scanner, or the slide set you are working with.
  • Page 123 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – Report sender settings Figure 117: Report sender settings Add new recipients to the list by clicking + or remove selected from the list by clicking -. To send an example mail to the selected recipient, click .

  • Page 124: Services

    PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES 6.2 Services Activate Service menu by clicking at the right side of the main menu bar. • – Service password Enter password first to enable service settings for modification. Figure 119: Enter service password •...
  • Page 125 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES Set Position, Objective type, and Magnification settings of the objective. Use manual camera changer – Changing the cameras is performed manually. Pre-scan objective protection enabled – If activated, slide area near the edges will not be scanned to record fast preview image.
  • Page 126 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES Notice: If Lumencor SPECTRA 6 is configured as illumination unit, the corresponding Enable Lumencor hardware trigger option is displayed on the Filter settings form. Stitching overlap – Set the amount how much the FOVs should extend over. Measure camera rotation –...
  • Page 127 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES On this panel, you can define which filters occupy which turret positions. The actually set filter position is marked at the Current filter hardware position field. Select filter hardware positions from the Filter hardware position: drop-down list. Notice: The maximum number of virtual filters is 50 if you insert Penta filters (and this type is selected from the Filter type list) in the available 10 filter positions in the turret.
  • Page 128 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – Z-difference This function is available only for fluorescence settings; it enables you to set the basic focus differences between the filters. This facilitates more precise focusing during scanning. Figure 122: Filter settings •...
  • Page 129 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES • – Barcode settings By predefining barcode type, foreground color (black or white – the color of the barcode), and other settings, you enable the Read Barcode option when using the Pannoramic® Scanner Software for Research control software Figure 124: Barcode settings To display an image of the barcode area, click .
  • Page 130 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES Click to check that barcode settings are correctly set. Click Reset barcode exposition time to load default exposition time used for barcode reading. Notice: If the figure is not set correctly, or there is no barcode in the barcode area, the software will display the following error message: “Barcode detection unsuccessful! There might be no barcode present.”.
  • Page 131 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES Notice: When having two barcodes on the slide, barcode layout must be consequential. • Resize image – Label image is downsized to 1:4 before barcode reading. • Apply erode algorithm – The Erode algorithm is run before barcode reading. It is a useful function for reading 2D barcode types since the algorithm completes the scuffed and faded areas of the barcode by applying the morphological erode filter to an image.
  • Page 132 PANNORAMIC® MIDI RX 1.0 SETTINGS AND SERVICES ▪ Barcode area light ▪ Backlight illumination intensity Objective guide ▪ Step size ▪ X / Y values or navigation arrows ▪ Goto ▪ Goto Preview Origin RGB LED ▪ Set illumination intensity per channel Sync with microscope Calculate white balance Save RGB intensity...

  • Page 133: Point Spread Function

    PANNORAMIC® MIDI RX 1.0 POINT SPREAD FUNCTION 7 Point Spread Function The Point Spread Function (PSF) is a light scattering pattern of the emission of a point-like object interpreted in three-dimensional space. The scanned image is the result of a convolution of the point object and the PSF.
  • Page 134 PANNORAMIC® MIDI RX 1.0 POINT SPREAD FUNCTION 4. Find the focal plane, then use Go within range and + -Range to set how many images the Scanner should take in the Z-direction. (The distance between the two images in Z- direction is 0.2 μm).

  • Page 135: Using Profiles

    PANNORAMIC® MIDI RX 1.0 USING PROFILES 8 Using Profiles Notice: Saved profile settings cannot be applied if using a different type of Pannoramic® scanner. If you want to scan a group of slides, it is advised to save your settings previously in a profile and continue scanning in Automatic mode.
  • Page 136 PANNORAMIC® MIDI RX 1.0 USING PROFILES Fluorescent settings • Filter position(s) • Selected focus channel • Selected stitching channel • Flat field correction (ON/OFF) Intensity statistics Intensity reducing Intensity equalization • Sharpening (ON/OFF) Other settings • Limitation of focus range (ON/OFF) •...

  • Page 137: Troubleshooting And Maintenance

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 9 Troubleshooting and Maintenance 9.1 Activation of the Control Software The activation of Pannoramic® Scanner Software for Research is required in order to use the service, otherwise the license validation cannot be performed despite possessing a valid Dongle or license file.
  • Page 138 PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 2) Activation web page is opened in the embedded browser. First accept the EULA, then click Continue. 3) Enter all the required data for the activation. If you do not possess a registration code, the Key field may be left blank.
  • Page 139 PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 4) Click Next to finalize installation (if the software is activated during installation), so the activation is performed, and the control software is ready for use. Offline activation 1) If the server is not connected to the internet, then Offline activation can be selected only.
  • Page 140 NOTE: The Next button is active only, if the file has successfully been saved. 3) The saved activation request file must be then copied to the device with internet https://activation.3dhistech.com web page. This step is connection, and uploaded to the mandatory only if Offline activation was selected.
  • Page 141 PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 9) Load this file in the following window (either by clicking Load..., or by drag&drop to the Drop file here... section of the window. Click Next to continue. NOTE: The Next button turns active only if file upload and validation was successful. 10) Upon successful activation the following window is displayed.

  • Page 142: Recovery Functions

    TROUBLESHOOTING AND MAINTENANCE If the license file could not be generated due to some reason, the below window appears, and in this case, you must contact 3DHISTECH Support at support@3dhistech.com. 9.2 Recovery Functions PSW automated backups allow you to continue scanning after unexpected events within as little time as possible.
  • Page 143 PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE Only plug the device in a socket with protective grounding (earth) contact. Do not operate the device in explosive environment. Disconnect the device from the power outlet before opening it or replacing a part. The product is not equipped with protection against hazardous materials (infectious, toxic, radioactive, corrosive substances or other effects damaging to health).

  • Page 144: Cleaning The Device

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 9.3.1 Cleaning the Device • Before cleaning, switch off the device and pull the plug out. • Do not let liquid penetrate into the system. • To clean liquid from the device, use a lint-free cotton cloth. You can moisten the cloth with water.
  • Page 145 Disable Limiting focus range function. Poor image quality • Imaging error (for example, chromatic aberrations) • Contact 3DHISTECH Service for support. Warning! Do not modify the camera driver settings, as the default factory settings give the most accurate images. MAY 13, 2021...

  • Page 146: Fluorescent Applications (Optional)

    TROUBLESHOOTING AND MAINTENANCE Virtual slides show poor stitching in Viewer. • Scan camera is not properly adjusted (for example, due to loose fixture) • Contact 3DHISTECH Service for support. Slide is often positioned incorrectly. • Guiding fault in transport system •...

  • Page 147: Maintenance

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE 9.5 Maintenance This section describes the most common maintenance solutions that can be performed by the user of the product. For maintenance solutions not described in this manual, contact the service personnel. 9.5.1 Opening the Protective Covers To perform any maintenance work and to interrupt a running digitization procedure because of slide or tray jamming, the protective covers of the Pannoramic®...

  • Page 148: Removing A Slide

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE Note: If necessary, one or both halves can be removed completely. For this purpose, push them to the right or to the left until they can be detached. On completion of the maintenance or repair work, the cover halves must be pushed together again and locked.

  • Page 149: Installing And Removing The Filter Module (Fluorescent Option)

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE Push protective covers apart (see chapter 9.5.1 Opening the Protective Covers). Move clamp (1) to the left, so the slide could be easily removed. While holding the clamp pushed to the left, remove the slide (2) carefully. Caution! If the slide is broken, make sure to remove all pieces completely from the device.

  • Page 150: Transporting Pannoramic® Midi Rx

    PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE Select the desired filter in the corresponding software window (see chapter Settings). The filter will be positioned in such a way that the user can access it without having to turn the filter wheel manually. Power off the scanner.
  • Page 151 PANNORAMIC® MIDI RX 1.0 TROUBLESHOOTING AND MAINTENANCE Caution! Be sure that the scan camera is not moved during transportation. To transport Pannoramic® MIDI RX do the following: Turn power supply off and detach all cables. Remove the slide tray if necessary. Transport Pannoramic®...

  • Page 152: Technical Data

    PANNORAMIC® MIDI RX 1.0 TECHNICAL DATA 10 Technical Data Dimensions (width x depth x height) Base unit approx. 900 mm (W) x 500 mm (D) x 500 mm (H) Base unit with fluorescence option approx. 900 mm (W) x 500 mm (D) x 500 mm (H) 24V 100W power supply approx.
  • Page 153 PANNORAMIC® MIDI RX 1.0 TECHNICAL DATA Operating data Intended site closed room facilities Electrical protection class Internal protection degree IP 20 in compliance with DIN EN 61010-1:2001, DIN EN 61010-2- Electrical safety 101:2002 (IEC 61010-1:2001, IEC 61010-2-101:2002) Over-voltage category Radio interference suppression in accordance with EN 55011 class B Noise immunity in accordance with IEC 61326-2-6:2012...
  • Page 154 PANNORAMIC® MIDI RX 1.0 TECHNICAL DATA Light sources Brightfield illumination Multichannel LED-based with wide Red, Green, Blue emission Average service life ~ 30000 hrs Fluorescent light source 1 (optional) SOLA SM (5 solid-state sources producing white light) Wavelength range 350–680 nm Average service life 20 000 hours Fluorescent light source 2 (optional)
  • Page 155 PANNORAMIC® MIDI RX 1.0 TECHNICAL DATA Scan cameras Point Grey Grasshopper 3 (GS3-U3-51S5M-C) Global Shutter Type CMOS (Sony IMX250) Pixel size 3.45µm × 3.45µm Camera resolution 2448 (H) × 2048 (W) pixel Frames rates 75 fps Bit-depth 10-bit / 12-bit Pixel resolution with 20x objective and 0.63x C-mount adapter 0.27µm Pixel resolution with 20x objective and 1x C-mount adapter...
  • Page 156 PANNORAMIC® MIDI RX 1.0 TECHNICAL DATA Pixel data rate 184.0 Mpixel/s Region of interest (ROI) horizontal: steps of 32 pixels vertical: steps of 8 pixels Trigger input signals frame trigger, acquire (SMA connectors) Trigger output signals exposure, busy (SMA connectors) Data interface USB 3.1 Gen 1 Time stamp...

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