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3DHISTECH TMA Grand Master 3.2 User Manual

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TMA Grand Master
3.2
User's Guide
August 6, 2020
GM32-UG-EN Rev1
Rev. 1

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Summary of Contents for 3DHISTECH TMA Grand Master 3.2

  • Page 1 TMA Grand Master User’s Guide August 6, 2020 GM32-UG-EN Rev1 Rev. 1...

  • Page 2: Table Of Contents

    TMA Grand Master 3.2 – User’s Guide Contents Disclaimer............................4 Declaraton Of Conformity......................6 Character Formats and Symbols....................7 Terms and abbreviatons......................8 1 Intended Use..........................9 1.1 Main Features of TMA Grand Master....................9 2 TMA Grand Master Hardware....................10 2.1 TMA Grand Master Main Unit......................10 2.2 Cabling..............................11...
  • Page 3 TMA Grand Master 3.2 – User’s Guide 5.4 Insertng Donor Blocks.........................45 5.5 Launcting tte transfer process......................47 5.6 Slide overlay............................57 5.7 Import data............................64 5.8 Restore medical informaton database....................67 5.9 Exportng donor block data witt TMA Register...................68 5.9.1 Export................................68 5.9.2 Searct................................77 5.9.3 Slides................................80 5.10 Batct removal of blocks........................82...

  • Page 4: Disclaimer

    IP are tte sole propertes of tte 3DHISTECH Ltd. 3DHISTECH Ltd. is not liable for damane of whatever nature (includinn, but not limited to, neneral or specific damane, indirect damane, consequental damane or incidental damane, includinn the...
  • Page 5 IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Further Informaton For tte latest informaton on 3DHISTECH products and services, please visit our website at tte following URL: ttp ://www.3dtistect.com Company Address: 3 Öv Street 1141 Budapest –...

  • Page 6: Declaraton Of Conformity

    Declaration of Conformity Declaraton of Conformity 3DHISTECH Ltd. declares ttat tte product TMA Grand Master is designed and produced witt consideraton of specifed requirements according to tte IEC 61326-2-6:2005 standard, and conforms to tte directves and otter standards listed below: IEC 61010-1:2001 (ed.

  • Page 7: Character Formats And Symbols

    TMA Grand Master 3.2 – User’s Guide Character Formats and Symbols Character Formats and Symbols Example Abbreviaton or term ttat is explained in tte secton Terms and Abbreviatons. Example Words or ctaracters ttat appear on tte screen. Ttese include feld names, screen ttles, pustbutons and menu names, patts or optons.

  • Page 8: Terms And Abbreviatons

    TMA Grand Master 3.2 – User’s Guide Terms and Abbreviations Terms and Abbreviatons Dinital slide / Slide Slide digitized witt a scanner microscope, for example Pannoramic SCAN. Consists of an MRXS fle and a folder witt tte same name as tte MRXS fle.

  • Page 9: Main Features Of Tma Grand Master

    TMA Grand Master 3.2 – User’s Guide 1 About TMA Grand Master 1 About TMA Grand Master TMA Grand Master is a sofware-controlled electronic laboratory device ttat automates tte creaton of tssue microarrays (TMAs) and also tte process of PCR sampling.
  • Page 10 TMA Grand Master 3.2 – User’s Guide 1 About TMA Grand Master 1.2 New Features Custom barcode informatonn barcode informaton can be used as an extra informaton • instead of donor/recipient name. If you ctoose to save it to tte database you can later view ttis informaton in your export fles.

  • Page 11: Tma Grand Master Hardware

    TMA Grand Master 3.2 – User’s Guide 2 TMA Grand Master Hardware 2 TMA Grand Master Hardware Hardware: TMA Grand Master main unit • Control computer witt a monitor, a mouse and a keyboard • USB cables between tte main unit and tte control computer •...

  • Page 12: Cabling

    TMA Grand Master 3.2 – User’s Guide 2 TMA Grand Master Hardware Back iew Figure 2 – TMA Grand Master back view 1. Terminal point for tte power supply cord 2. Main power supply switct 3. Fuse compartment 4. USB ports to connect witt tte computer.

  • Page 13: Delivery

    TMA Grand Master 3.2 – User’s Guide 2 TMA Grand Master Hardware Warninn! Only connect external devices ttat are safety extra low voltage rated to tte TMA Grand Master instrument to avoid tte risk of electrical stock. 2.3 Delivery Packane 1 TMA Grand Master main unit (packed in a mandatory polyettylene container in a •...

  • Page 14: Transportng Tte Tma Grand Master Main Unit

    Warninn! Only qualifed personnel may install TMA Grand Master and perform tte inital start-up procedure. 3DHISTECH Service tectnicians install and confgure TMA Grand Master and tand it over to you in operatng conditon. 2.5 Transportnn the TMA Grand Master Main Unit...
  • Page 15 TMA Grand Master 3.2 – User’s Guide 2 TMA Grand Master Hardware 3. Lif tte main unit by its recessed transportng tandles ttat can be found bott sides of tte botom plate, underneatt tte side-plates of tte main unit. Figure 4 – Transporting handle 4.

  • Page 16: Installaton Of Tma Control Sofware

    500 GB – for saving tte TMA database, and for storing digital slides TMA Control Sofware Installaton Important! 3DHISTECH service tectnicians perform tte installaton and confguraton of tte TMA Control sofware. Tte following installaton procedure is for informaton purposes only – follow tte below steps only if you need to re-install tte sofware, or upgrade to a newer sofware version.
  • Page 17 TMA Grand Master 3.2 – User’s Guide 3 Installation of TMA Control Software 1. Launct tte TMAControl_3.2_RTM_x64.exe fle ttat you received on a 3DHISTECH Installaton Disc or downloaded in a compressed fle. 2. Follow tte instructons in tte installaton wiizard.
  • Page 18 TMA Grand Master 3.2 – User’s Guide 3 Installation of TMA Control Software 4. Tte installer notfes you if it cannot fnd tte prerequisites. Click Install to start tte installaton of missing prerequisites. 5. Click Next to contnue witt tte installaton of tte TMA Control sofware applicaton.
  • Page 19 TMA Grand Master 3.2 – User’s Guide 3 Installation of TMA Control Software 6. Select tte type of license – browse for tte license fle or select HASP dongle. 7. Ctoose tte installaton folder for tte TMA Control sofware applicaton. Click Browse for locatng a diferent folder ttan tte default one, tten click Next to contnue.
  • Page 20 TMA Grand Master 3.2 – User’s Guide 3 Installation of TMA Control Software 8. Ctoose tte Start menu folder. 9. Afer completng tte installaton of TMA Control, click Finish to close tte wiizard. Important! Afer tte installaton, RW access rigtt to database and confguraton fle must be granted for Admin and Standard users.

  • Page 21: Operatnn The Main Unit

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 4 Operatnn the Main Unit 4.1 Switchinn the Main Unit On or Of Turninn the Power On 1. Switct on tte TMA Grand Master (frst, tte main switct on tte back panel – see Figure 2 (2), tten tte one in tte front –...
  • Page 22 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit Warninn! You receive a warning message afer startng tte sofware, if not taving a valid license for running tte applicaton. A separate license is necessary to actvate tte PCR functon of tte TMA Control sofware.
  • Page 23 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit To load an existng project, tte following window is displayed afer clicking Load, in wtict you can select tte desired project to be loaded, and narrow project list by actvatng Date Ranne and/or Diameter flters.

  • Page 24: Main User Interface

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 4.3 Main User Interface Ttis version of TMA Control ofers two diferent views of recipient and donor block panels. Based on tte loading and processing mettod of blocks tte normal view includes ttumbnails of all recipient tray positons, and as for donor blocks tte selected tray is displayed in its full capacity.

  • Page 25: Main Toolbar

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit If you want to load a recipient block into tte unit, click at tte Unused Block(s) panel teader. As for donor blocks, frst click tte buton, tten if ttere is a free slot on tte actual tray, tte door can be opened (otterwise wait untl tte closest tray witt a free slot on it is rotated to loading positon) and tte block can be inserted.
  • Page 26 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit - Settinns window contains tte setngs for some important features in TMA Control, including Overlay, Annotaton, Restore Medical Info Database, Deptts, Images, Layout PCR Setngs, Barcode, Tteme and Language. See ctapter 6 Settgs for more informaton.
  • Page 27 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit Tutorial ideos – Selected tutorial videos are listed and can be opened by clicking • tte links. NOTEn Before opening tte videos, make sure to copy video fles to <C:\Program Files\3DHISTECH\TMA Control\Videos\TMA Grand Master>...

  • Page 28: Insertng And Removing Blocks

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 4.4 Insertnn and Removinn Blocks 4.4.1 Block Preparaton Warninn! If tte parafn swells ttrougt tte botom or tte sides of tte plastc cassete, cut of tte excess parafn before insertng tte block into tte main unit.

  • Page 29: Cleaning Block And Pcr Cassete Preparaton

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 4.4.2 Cleaninn Block and PCR Cassete Preparaton Important! Before insertng a PCR cassete into its slot, ensure ttat tte cover plate and tubes are uncontaminated. One PCR cassete can be inserted into its slot before tte frst positon on eact donor tray.

  • Page 30: Insertng A Block Into Tte Main Unit

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit Important! Before startng a larger project it is advised to calculate tte number of cores ttat will be inserted to PCR tubes, so placing a sufcient number of cleaning blocks accordingly.
  • Page 31 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 2. For tte recipient blocks (1) click tte (Channe Blocks) buton next to tte image of tte specifc block pairs. Depending on tte donor block tray ttat you want to rotate to insertng positon, click tte tray ID: A –...

  • Page 32: Removing A Block From Tte Main Unit

    TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 4.4.4 Removinn a Block from the Main Unit 1. Recipient block positons are displayed in a double-column on tte lef side of tte screen. Donor block tray positons are identfed by six butons (A-F). Tte (Channe Blocks) butons are responsible for rotaton of tte donor block tolder cylinder.
  • Page 33 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 3. Open tte tool exctanger compartment door. Figure 7 – Tool compartment 4. Loosen tte drill ctuck locking lever (marked witt red) ttat fxes tte drill ctuck in positon and remove tte drill ctuck by lifing it upwards.
  • Page 34 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 5. Hold tte front cog wteel frmly and loosen tte rear cog wteel (marked witt red) and turn it antclockwise about two turns (360-720 degrees), tten remove tte drill bit from tte drill ctuck.
  • Page 35 TMA Grand Master 3.2 – User’s Guide 4 Operating the Main Unit 10. Pull out tte piston, tten told tte front cog wteel frmly and unscrew tte rear cog wteel (marked witt red) by turning it antclockwise about two turns (360-720 degrees), and now you are able to remove tte puncter.

  • Page 36: Usinn Tma Grand Master

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5 Usinn TMA Grand Master Ttis TMA Grand Master device is designed to create TMA blocks and make it available for you to place extracted cores into PCR tubes at tte same tme, wittin tte same process. Witt tte operatng sofware you may set processes for creatng TMA blocks or PCR sampling exclusively or bott.

  • Page 37: Before You Start

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.1.1 Before You Start Warninn! Do not use parafn blocks wtict dimension values are tigter ttan specifed (24mm (W) x 37mm (H) x 10mm (D)), otterwise tte drilling and puncting teads may be damaged.
  • Page 38 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn Ttere is a icon at tte botom of ttis buton, and if you click it tte layout will be deleted. New layout (TMA block array) can be created or edited in tte Layout Editor window. Already existng layout can be selected from tte Select Layout drop-down list.
  • Page 39 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Columns, rows, or feld selecton: wtile keeping tte mouse buton pressed, select • tte unwanted area on tte layout. Figure 12 – Layout pattern If you actvate tte Use Sectors functon, tte layout can be divided into several sectors. Mark •...
  • Page 40 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Tte flling of sectors will be processed uniquely during TMA block creaton, one sector is • completed at a tme, tten tte device processes tte upcoming sector you tave created.
  • Page 41 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master It is also available to use dividing rows and columns in tte block by leaving actve spots • between tte sectors ttat can be flled up witt Agarose or otter tssue (for example, liver) ttat difers in color from tte core tssue.

  • Page 42: Modifying A Recipient Block Layout

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Important! Tte table below stows tte maximum number of toles can be drilled into a parafn block depending on tole siizes: Paraffin Block Siie (mm) Hole Siies (mm) Max.

  • Page 43: Deletng A Recipient Block Layout

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.2.3 Deletnn a Recipient Block Layout If you wist to delete tte layout for tte selected block, eitter click next to tte block image 1. Open tte Layout Editor window.
  • Page 44 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 4. Select a layout for tte recipient block: a) Click tte image of tte recipient block for wtict you want to add a layout: b) Select a pre-defned layout from tte Select Layout drop-down list in tte Layout Editor window, tten click Use this layout.
  • Page 45 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Important! If loading a PCR cassete in tte tray, make sure to insert tte necessary amount of cleaning blocks into recipient block positons. 5. Tte recipient block barcode is automatcally identfed, but if not recogniized properly, tte following message appears: Click Yes, and tte Barcode window is opened in wtict you can modify setngs.

  • Page 46: Insertng Donor Blocks

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.4 Insertnn Donor Blocks 1. Insert donor blocks into tte frst tray, positons A1-10. For more informaton see secton 4.4.3 Itsertitg a block itto the mait utit. Warninn!
  • Page 47 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Furttermore, if otter locatons tave been defned in tte Setngs window for slide images to be superimposed, more images can be found witt an identcal ID. See secton 6 Settgs for more informaton.
  • Page 48 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Barcode informaton can be used as an extra informaton instead of donor/recipient name. If you want to use ttis feature, select Custom Infomaton in tte Barcode label content type secton.

  • Page 49: Launcting Tte Transfer Process

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.5 Launchinn the Transfer Process Warninn! Do NOT ever atempt eitter to remove tte parafn container or to open tte service compartment door during operaton. Warninn! PCR process is not supported in case tte sofware was restarted afer exitng wittout closing it properly or loading tte project ttat tas been saved previously.
  • Page 50 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn Tte frst tube positon must always be on tte lef botom of tte cassete, and tte cover plate tab faces to tte lef side of tte device. Afer you tave actvated tte PCR functon by clicking tte PCR buton, make sure to insert cleaning blocks (or mark a sufcient amount of recipient block as cleaning blocks).
  • Page 51 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Sometmes it is required to alter image setngs suct as brigttness, contrast, or gamma to make label images more identfable or donor and/or recipient block images appear visually beter for identfying...
  • Page 52 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn You can always list bott tte donor block and tte recipient informaton by clicking tte Informaton buton in tte top rigtt corner of eact view. Before startng tte transfer process, follow tte sequence of steps below to place markers on donor blocks from wtict locatons cores will be extracted to tte selected recipient positons or PCR tubes.
  • Page 53 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 2. Wten trying to place a PCR marker on tte donor block, and you tave not yet entered any TubeID for tte tubes, a message appears: 3. Enter Tube IDs into tte corresponding felds. Hit Enter to accept IDs, and click OK to exit from tte PCR Tube ID Editor window.
  • Page 54 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5. Tte number of PCR markers placed on tte donor block appears in tte PCR buton. Place TMA markers on desired areas of tte donor block wtere tssue core stould be extracted from, tten click tte positon of tte recipient block layout ttat you want to fll witt tte core sample.
  • Page 55 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master If you tave not entered Recipient block ID, nor Donor block ID for tte selected transfer, a window opens in wtict you must enter tte Ids. TMA Grand Master inserts eact core into tte next available spot, and proceeds from lef-to- rigtt and from top-to-down order in tte layout grid of tte TMA block.
  • Page 56 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 8. During tte process, tte colors of tte cores on tte layout varies according to tteir statuses. Color markings of cores and tteir statuses are as follows: – transferred;...
  • Page 57 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Wten you place tte cursor over tte locaton of an extracted core on tte donor image, tte spot on tte recipient block is marked by tte center point of tte blue cross-tair. On tte otter...
  • Page 58 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Afer saving, tte Switch Layout/Spots icon appears in tte botom-rigtt corner of tte recipient image. Click ttis icon to open tte spot slide view of tte recipient block. By moving tte cursor over a core wtere a spot slide is applied, a spot slide preview image is displayed.
  • Page 59 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn You will need CaseViewer and Slide Converter tools installed on your computer to take advantage of ttese functons. Re-puncting of cores is available for you (can be initated bott at tte donor and tte recipient •...
  • Page 60 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Click Yes in tte pop-up window to delete ID and tte markers of tte previously used donor block. If you remove a donor block to wtict cores are selected to be transferred, tte sofware displays a message to warn you to place tte block back to its positon.

  • Page 61: Slide Overlay

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Important! If you replace tte donor block but tte PCR tube for tte given positon remains in tte cassete and additonal cores will be selected for PCR extracton, tte PCR tube will contain cores from diferent blocks.
  • Page 62 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Prior to superimposing a slide on tte donor image, TMA Markers can be placed on tte slide in CaseViewer witt tte TMA Marker functon (refer to ctapter 3.2 Futctiots – 6. Attotatiot mode in CaseViewer User's Guide).
  • Page 63 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 3. If connected to a CaseCenter server, tte images of tte related case will be displayed. If more ttan one images are found in tte set locatons (slide, JPG, PNG, BMP, TIFF, SVS, CC) tten select one image from tte images displayed in tte Select Slide window.
  • Page 64 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master To connect to a dedicated CaseCenter server click CC connect. First, select server address, tten enter your user name and password before clicking OK in tte CaseCenter Lonin window.
  • Page 65 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 7. Slide image is rotated to tte appropriate orientaton automatcally, but if you would like to select tte matcting slide image witt a diferent automatc orientaton, click at tte top of tte overlay editor buton.
  • Page 66 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 9. In tte appearing Manual Overlay window, imported slide image is displayed at tte lef and as superimposed on tte donor image at tte rigtt, accordingly, additonal functons suct as Opacity, Zoom, Rotate, and Flip are available for you in tte extended panel to set tte proper orientaton.
  • Page 67 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5. If you want to reset tte image afer dragging tte ttird reference point, click Use three reference points again. NOTEn Afer placing tte reference points, bott tte overlay image and tte reference points can be moved by dragging.
  • Page 68 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master If you click tte >> icon and close tte overlay window, tte TMA markers will be displayed • on tte donor block image in tte main window. Confrm marker positons by clicking on ttem. Close spots will be automatcally dragged •...
  • Page 69 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn As marker colors and tteir names will be displayed in tte relatng cells of tte Annotaton iolor column on tte export steet, to omit tte usage of ttose colors ttat can...

  • Page 70: Import Data

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn Tte annotaton visualiizaton can be turned of in tte Settinns, and also tte siize of markers to display can be selected on tte Annotaton panel. By default ttose TMA markers appear on tte donor image ttat matct tte siize of tte actual tool inserted.
  • Page 71 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 2. Click Browse in tte Import window to searct for tte data fle to be imported. If tte ID of tte donor block loaded into tte device exists in tte imported database, tte data set of tte specifc donor block can be appended to tte database.
  • Page 72 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 4. Select Replace to overwrite existng data witt tte new ones. In ttis case, tte already existng data is arctived and can be accessed afer clicking Settinns. Click Restore in tte Setngs window to restore tte original data set (see ctapter 6 Settgs for more informaton).

  • Page 73: Restore Medical Informaton Database

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 6. Diferences in tte data (for example, if donor data of a specifc block do not matct – name, age, etc.) are tigtligtted witt red in tte window.
  • Page 74 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.9 Usinn TMA Renister 5.9.1 Lonin If you open TMA Register from TMA Control and you are not logged in to tte CaseCenter server, you can log in ttrougt tte TMA Renister window.
  • Page 75 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master TMA Slides • In tte Auto TMA Slide Search in CaseCenter feld, you can pair tte recipients slide informaton witt tte TMA slides located on tte CaseCenter in 3.1 RTM. You can Set CaseCenter slide folders for TMA slides to matct tte overlay slides folder setngs, but you can ctoose diferent folders, too.
  • Page 76 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 6. Click tte Slides page and select recipient. 7. List tte TMA slides in tte Slide Name column. 8. Save tte table. 9. Click tte Search TMA Slides in CC buton.
  • Page 77 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master irtual Spot Slides Folder Structure in CaseCenter Tte Virtual Spot Slides are located in tte main folder named TMAControl_SpotSlides. In ttis folder, you can fnd tte Reiipients folder to group your recipients. Eact Reiipients folder can contain 200 recipients.

  • Page 78: Export

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master For virtual slide management in CaseCenter, see CaseCetter 2.9 SP2 User's Guide. Searchinn CaseCenter irtual Spot Slides Searcting CaseCenter virtual spot slides is available directly from tte Search menu. Searct for your recipient in Core Info view and click on tte links in tte CC Spots column.
  • Page 79 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Creaton tten set Start Date and End Date for more fltering opton. Click to set date in a pop-up calendar window. Sctemes can be defned based on database-driven project tandling by creatng a scteme or selectng one from tte Scheme drop-down list.
  • Page 80 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 2. Moving blocks from tte Recipient Blocksn feld to tte Coherent Blocksn you can select ttem by ctecking tte boxes next to eact item. Click tte buton to move tte selected blocks to separate Coherent Groups.
  • Page 81 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Tte Recipient Block Imane and Donor Block Imanes columns lists image links (images stored in separate folders at tte same locaton as tte spreadsteet fle, and donor image fle names receive tte project name and block ID).
  • Page 82 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master IV. An Informaton steet includes all tte following data: TMA block name ◦ Heigtt (recipient actual teigtt) ◦ Project name ◦ Creaton date ◦ Modifcaton date ◦ Diameter ◦...
  • Page 83 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Eitter in mixed mode, or if cores tave been transferred to PCR tubes only, actvate Save PCR data opton to create a separate fle for PCR-related data. Enter database-related parameters (Name, Path, Type) and browse for tte locaton of tte database fle you are about to create.
  • Page 84 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master On tte By_Sample_Core steet you can fnd PCR data sorted by tube in tte following ◦ columns: Donor Block ID, Donor Block Images, Donor Core Heigtt, Spot slide, PCR Tube ID, Tube (e.g.: '2/4, PCR Tube 7'), Annotaton Color, and Annotaton name.
  • Page 85 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master By_Annotaton_Color steet lists data by cores, but data are sorted by annotaton color ◦ per donor. Ttis steet is created only if at least one annotaton color is present in tte export parameter list.
  • Page 86 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master C. In “full” mode (if you tave selected tte Free PCR sampling mode and you tave added IDs to tte tubes, tte project includes data imported from Medical Informaton database, and ttere...
  • Page 87 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master 5.9.4 Search Tte TMA Register window includes a Search panel ttrougt wtict TMA data stored in tte database can be fltered, and you can searct for Date, Project name, Recipient Block Name, Donor Block Name, Diameter, TMA Slide and Medical Informaton data.
  • Page 88 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn Wten selectng tte Donor name, PCR donor teigtts are not listed if tte data are created witt a previous sofware version (older ttan v2.7) If Block info buton is actve, and Diameter flter opton is added, results are listed according to Recipient name in tte frst column.
  • Page 89 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master At tte lef of tte window a set of spots extracted from tte donor block, a so-called “spot slide” is visible (images are taken from tte marker annotatons of tte digital slide).

  • Page 90: Slides

    TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master NOTEn If an additonal flter is added, tte previously set flter setng will be inactve and cannot be modifed. 5.9.5 Slides Tte TMA Register feature enables you to append variuos TMA Slide data, suct as slide name, stain, organ, etc.
  • Page 91 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master In ttis view, tte image of tte recipient block can be displayed afer selectng tte corresponding block from tte list. Block propertes secton contains several data suct as: Layout name •...
  • Page 92 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master Data entry is performed in a table, in wtict columns and containing data can be ordered, and are freely variable and editable. A new, empty table can be created by clicking Important! Column teaders (i.e.
  • Page 93 TMA Grand Master 3.2 – User’s Guide 5 Using TMA Grand Master As an opton, you can export TMA Slide data to tte following fle formats: ODS, XLS, XLSX, and CSV at tte botom of tte window. First enter database name into tte top feld, tten patt to tte botom feld, fnally select fle format, and click Export.

  • Page 94: Settinns

    TMA Grand Master 3.2 – User’s Guide 6 Settings 6 Settinns Tte Setngs window contains ten panels on wtict functon-related optons can be actvated and furtter setngs can be modifed. Overlay Slide superimpositon setngs can be modifed on tte Overlay panel of tte Setngs window.
  • Page 95 TMA Grand Master 3.2 – User’s Guide 6 Settings Annotaton Annotated areas (suct as TMA markers and closed annotatons) of tte slide can be displayed on tte donor image. Actvate Show TMA Marker annotatons from slide opton and select tte siize of marker to be displayed (tte marker siize identcal witt tte siize of tte actual tool inserted is selected by default).
  • Page 96 TMA Grand Master 3.2 – User’s Guide 6 Settings Depths Inserton depth (μm) • Deptt of inserton into tte recipient block can be set eitter by setng a negatve or a positve value, so ttat tte tp of tte puncter is positoned below or above tte surface of tte block insertng tte core at a required teigtt.
  • Page 97 TMA Grand Master 3.2 – User’s Guide 6 Settings Imanes Upon actvatng tte Show imane-related functons opton, four butons will be displayed on tte botom rigtt corner of tte block images. – Slide Open • For donor blocks wtere slide overlay is applied, you can eitter open tte original MRXS slide in CaseViewer or tte associated program for image slides by clicking on ttis buton.
  • Page 98 TMA Grand Master 3.2 – User’s Guide 6 Settings Layout Layout numbering directon and row/column labeling setngs are presented in ttis panel. Labelinn display • Actvate tte Show row and column labelinn on layout opton to display labels in layout editor and on tte recipient block image.
  • Page 99 TMA Grand Master 3.2 – User’s Guide 6 Settings PCR Settinns On tte PCR Settinns panel, two panes are available: NOTEn Ttese panes are actve only if ttere is no project open. PCR Inserton Mode • Here, you can select Conventonal PCR core inserton or Freely selectable PCR core inserton modes.
  • Page 100 TMA Grand Master 3.2 – User’s Guide 6 Settings Barcode Tick Enable automatc functon to set tte identfable type of barcode ttat is specifed manually (select Manual) or actvate Autodetect to allow tte program tte automatc identfcaton wittout specifying tte barcode type, ttus blocks witt any kind of barcode will be identfed during creatng a project.
  • Page 101 TMA Grand Master 3.2 – User’s Guide 6 Settings For sofware versions older ttan 2.7 tte default barcode reader is selectable afer tte upgrade. Select intaSof to process barcodes witt tte embedded reader or Matrox to work witt MIL dongle.
  • Page 102 TMA Grand Master 3.2 – User’s Guide 6 Settings Lannuane Tte language of tte sofware can be selected from tte list. August 6, 2020 – Rev. 1 3DHISTECH Ltd. 102(116)

  • Page 103: Maintenance Of Tma Grand Master

    TMA Grand Master 3.2 – User’s Guide 7 Maintenance of TMA Grand Master 7 Maintenance of TMA Grand Master Follow tte maintenance and cleaning tasks discussed in ttis secton to ensure tte fail-safe operaton of tte TMA Grand Master device.

  • Page 104: Opening Tte Service Compartment Door

    TMA Grand Master 3.2 – User’s Guide 7 Maintenance of TMA Grand Master 7.2.1 Openinn the Service Compartment Door Important! NE ER open tte service compartment door or tte trast container during tte operaton of tte TMA Grand Master. Service compartment migtt be cleaned in order to remove dust or objects dropped in. To remove tte door of tte service compartment do tte following: 1.

  • Page 105: Replacing Fuses Of Tte Main Power Switct

    TMA Grand Master 3.2 – User’s Guide 7 Maintenance of TMA Grand Master 7.3 Replacinn Fuses of the Main Power Switch 1. Switct of tte main power supply and disconnect tte power cable. 2. Tte fuse compartment is located next to tte main power supply switct at tte back side of tte TMA Grand Master.

  • Page 106: Contaminated Tray Or Touct Sensor Surface

    TMA Grand Master 3.2 – User’s Guide 7 Maintenance of TMA Grand Master 5. Replace fuses, put tte insert back into its mountng, and pust it inwards untl you tear a snap, so tte lever locks. Figure 17 – Replacing the fuses Important! Use T3.15A/250V fuses only.
  • Page 107 TMA Grand Master 3.2 – User’s Guide 7 Maintenance of TMA Grand Master Afer performing tte above actons, and you tave closed tte door, tte touct sensor must be • cleansed as well. According to tte instructons in tte following message, by using a dry and clean clott, gently rub tte botom surface of tte touct sensor (sensor is marked witt red in Finure 18).

  • Page 108: Troubleshootnn Tma Grand Master

    TMA Grand Master 3.2 – User’s Guide 8 Troubleshooting TMA Grand Master 8 Troubleshootnn TMA Grand Master 8.1 Hardware-Related Issues TMA Grand Master Does Not Work at All What to don Contact 3DHISTECH support. Error Durinn Initaliiaton (Maintenance issue) Causen Tte surface of tte tray between tte positons F9 and F10 or tte touct sensor is contaminated.

  • Page 109: Sofware-Related Issues

    TMA Grand Master 3.2 – User’s Guide 8 Troubleshooting TMA Grand Master 8.2 Sofware-Related Issues Windows 7 Update If Windows 7 Update is launcted during working witt TMA Grand Master, make sure not to restart Windows 7 afer tte update process is fnisted. Follow tte below steps to avoid tte automatc reboot...
  • Page 110 HDD is inaccessible/not writable Tte System Administrator must ensure accessibility by tte control sofware to some folders on tte computer. For instance, write access must be granted to: C:\ProgramData\3DHISTECH\TMAControl folder, its sub-folders/fles, and in additon, enable tte sofware to modify registry logs.
  • Page 111 TMA Grand Master 3.2 – User’s Guide 8 Troubleshooting TMA Grand Master Block imane and label area not visible Causen Problem witt preview camera – COM driver registry entry is missing Solutonn Restart GTMA sofware applicaton. If tte problem stll persists, try to reinstall it.

  • Page 112: Cassete And Block Dimensions

    24 mm (H) x 37 mm (W) x 7.5 mm (D) • NOTEn If using tte 3DHISTECH block moulder, tte ttickness (deptt) of tte block is 13.5 mm. Tte smaller siize (7.5 mm in deptt) means tte parafn ttickness from tte upper edge of tte •...

  • Page 113: Technical Descripton Of The Hardware

    TMA Grand Master 3.2 – User’s Guide 10 Technical Description of the Hardware 10 Technical Descripton of the Hardware 10.1 Physical Dimensions and Weinht Name of the Unit Dimensions in mm* Weinht in kn* (Width x Depth x Heinht) TMA Grand Master main unit 807×505×444...

  • Page 114: Objectve And Cameras

    TMA Grand Master 3.2 – User’s Guide 10 Technical Description of the Hardware 10.3 Objectve and Cameras Component Descripton Objectve 16 mm focal lengtt Camera type VR Magic Camera resoluton 3,14 Million Pixel 10.4 Ambient Conditons Conditon Suitable Ranne Shipment (packed) Ambient temperature -40°C to +70°C...

  • Page 115: Operatng Data

    TMA Grand Master 3.2 – User’s Guide 10 Technical Description of the Hardware 10.5 Operatnn Data Catenory of operatnn environment closed room facilites Electrical protecton class Internal protecton denree IP 20 Electrical safety in compliance witt EN 61010-1 (IEC 61010-1) and taking CSA &...

  • Page 116: Index

    TMA Grand Master 3.2 – User’s Guide Index Index Barcode............................46, 47 Block............................48, 55 Donor block......8, 9, 13, 30, 31, 35, 45, 46, 47, 50, 51, 53, 54, 60, 62, 67, 69, 70, 90, 92 Drill................9, 12, 30, 31, 32, 33, 35, 36, 37, 41, 87, 96, 97 Excel............................13, 69...

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