Define The Plate Layout; Key To Control And Standards Used In Plate Layout; Press Enter To Move To The Next Method Definition Box; Press Enter To Get To The Next Method Definition: Standards - biochrom ASYS EXPERT PLUS Quick Start Manual

 For kinetics: Press F4 to select interval in seconds and number of readings using the
number key pad. Press F4 to confirm choices or F3 to discard.
If shaking and/or kinetics options were selected, the filters method definition window will
show ON after shaking and/or kinetics.
8. Next, define the plate layout. For controls, standards and samples, replicate direction can be
selected under F3, Options. F1 selects the type, F3 selects horizontal or vertical replicates
and F4 positions control in the plate. Press place to select the well of choice. Continue the
plate layout is completed. Use the backspace arrow to erase choices.
Code
BK
NC
PC
LP
HP
CO
QC
ST
 Samples can be placed either individually by moving the cursor to the desired
location or all wells can be filled with individual samples by selecting F2, Fill All.
9. Press enter to move to the next method definition box. If a kinetic measurement was
selected in a previous option, the kinetics definition options will be displayed (if not continue
on). Select enter to reach next window.
10. Press enter to get to the next method definition: standards. Use this screen to enter the
values of the standards, the units for the concentration and to choose the curve-fit method.
Press enter.
 Choose the F1, Quick, for entering standards using a dilution or multiplication factor.
12. If measurements are to be analyzed, select method to be used. Options include:
Method
Factor
July 2010
Table I. Key to Control and Standards used in Plate Layout
Control Type
The absorbance of the blank is automatically subtracted
Blank
from the absorbance of all other wells on the plate.
Negative control Use for qualitative/screening tests
Positive control Use for qualitative/screening tests
Low positive Use to define range of positive samples
High positive Use to define range of positive samples
Control Use as required
Quality control Use as required
Use for quantitative determinations; a maximum of 16
Standards
standards can be used.
When to Use:
If a batch-specific calibration factor is used
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When to Use:
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