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Biochrom EZ Read 400
Biochrom Ltd
22 Cambridge Science Park,
Milton Rd, Cambridge CB4 0FJ UK
Tel: +44 (0)1223 423723
Fax: +44 (0)1223 420164
Email: enquiries@biochrom.co.uk
www.biochrom.co.uk
Biochrom EZ Read 400 User's Manual
Microplate Reader
User's Manual
Version 2.1
Biochrom US
84 October Hill Road,
Holliston, MA 01746-1388 USA
Tel: (Toll free): 877-BIO-CHROM (877-246-2476)
Fax: 508-429-5732
Email:
sales@biochrom-us.com
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Table of Contents
Related Manuals for biochrom EZ Read 400
Summary of Contents for biochrom EZ Read 400
- Page 1 84 October Hill Road, Milton Rd, Cambridge CB4 0FJ UK Holliston, MA 01746-1388 USA Tel: +44 (0)1223 423723 Tel: (Toll free): 877-BIO-CHROM (877-246-2476) Fax: +44 (0)1223 420164 Fax: 508-429-5732 Email: enquiries@biochrom.co.uk Email: sales@biochrom-us.com www.biochrom.co.uk Biochrom EZ Read 400 User’s Manual Version 2.1...
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Page 2: Table Of Contents
Table 1: Rated Operating Conditions ................9 Unpacking the EZ Read 400 ..................10 Powering up the EZ Read 400 Microplate Reader ............ 10 3.10 Cleaning and Disinfecting the Instrument ..............11 3.11 Emergency Instructions ..........Error! Bookmark not defined. - Page 3 Contact Information ....................19 Table 3 Contact Information ..................19 Appendix: ........................ 20 Helpful Hints for Successful Microplate Assays ............20 Quick Start Guide ...................... 21 Troubleshooting and Frequently Asked Questions ........... 23 Biochrom EZ Read 400 User’s Manual Version 2.1...
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Page 4: Declaration Of Conformity For Biochrom Manufactured Products
Declaration of Conformity for Biochrom Manufactured Products This is to certify that the following Biochrom manufactured products conform to the requirements of the following Directives: EZ Read 400 Elisa (80-4001-40), EZ Read 400 Research (80-4001-41) and EZ Read 400 Flexi (80-4001-42) 2006/95/EC... -
Page 5: Introduction And Intended Use
400-750 nm in optically clear, flat-bottomed 96-well plates. Only trained laboratory personnel should operate the Biochrom EZ Read 400 Microplate Reader. The Biochrom EZ Read 400 Microplate Reader is intended for general laboratory and research use only. Biochrom EZ Read 400 User’s Manual... -
Page 6: Safety Information
Use the equipment only in the intended manner. Chemical and Biological Safety Normal operation of the Biochrom EZ Read 400 Microplate Reader may involve the use of materials that are toxic, flammable, infectious or otherwise biologically harmful. This general purpose laboratory equipment could be used with IVD samples and as such can be considered to be IVD medical equipment. -
Page 7: Safety Issues Regarding The Use Of A Pc With The Instrument
Dispose of all waste solutions safely and in accordance with the policies and practices of your facility. Operate the Biochrom Anthos Microplate Reader in accordance with the instructions outlined in this manual. Use appropriate precautions when using pathological, toxic, or radioactive materials. -
Page 8: Instrument Inputs And Outputs
Place plate here prior to measurement. Figure 1 Front of the EZRead 400 Microplate Reader On/Off Switch Power Supply Input USB A to B cable Input Figure 2 Back of the EZREAD 400 Microplate Reader Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 9: Instrument Validation
Spectral width at half maximum 8 nm intensity Ventilation There are no special ventilation requirements Warming-up time None required Wavelength accuracy 2 nm <6 kg. Does not require any special lifting or carrying Weight instructions Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 10: Unpacking The Ez Read 400
Place the device on a suitable working surface Remove the transportation lock (foam part) from the plate holder Powering up the EZ Read 400 Microplate Reader Connect the USB A to B cable from the PC to the instrument. Connect mains lead to the instrument mains inlet. -
Page 11: Cleaning And Disinfecting The Instrument
3.12 Safe Disposal of the Instrument This symbol can be found on the outside of the instrument. It means that the Biochrom EZ Read 400 microplate reader cannot be disposed of in municipal waste. The instrument must be decontaminated as detailed in part 2.8 of this section. -
Page 12: Regular Maintenance
3.13 Regular Maintenance The EZ Read 400 microplate reader does not require regular maintenance for successful use unless it is used in a manner that is outside of the recommendations laid out in this manual or stored in an environment that is excessively dusty or humid. Please contact your distributor for any questions or concerns regarding the performance of the instrument. -
Page 13: Software Installation And Use
4 Software Installation and Use Software Installation ADAP Basic is supplied with all EZ Read 400 instruments. The software can be used to control the reader to perform endpoint, kinetic and multiwavelength assays. Data analysis options are available with a software upgrade to ADAP Plus or ADAP Expert (See www.biochrom.co.uk for more information regarding ADAP Microplate Software). -
Page 14: Endpoint Measurements
(the minimum interval time is 45 seconds). Select Cycles for the number of measurements that will be made in total. Select Data Reduction method for analysis and Smoothing points, Upper and Lower Limit.* Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 15: Multiwavelength Measurements
Edit menu to select specific rows, columns for measurement. Specific wells can also be selected by highlighting the desired wells and then selecting Set Selected Wells. If the incorrect wells were selected simply select De-select all wells to start again. Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 16: Calibrating The Instrument
Calibrating the Instrument The EZ Read 400 performs an automatic calibration of the lamp energy before it measures each well. However if the user would like additional confirmation of the performance of the instrument; a calibration plate containing a series of neutral density filters with known optical density can be purchased to verify reader performance. -
Page 17: General Features
In its original condition, the instrument meets all safety regulations for safe operation. If the instrument is used in a manner not specified in this user’s manual, safety and performance may be affected. Biochrom is not liable for damages or costs caused by unauthorized alterations, repairs or modifications of the equipment. -
Page 18: Ordering Information And Accessories
Biochrom EZ Read 400 Flexi Microplate Reader B032082 ADAP Plus Software B032083 ADAP Expert Software S01087 Anthos 2010 Reader Check Plate See www.biochrom.co.uk for a list of filters that are available for purchase. Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 19: Contact Information
+44 (0) 1223 427890 Orders orders@biochrom.co.uk +44 (0) 1223 427861 +44 (0) 1223 420 164 (Fax) Returns returns@biochrom.co.uk +44 (0) 1223 427861 Human Resources hr@biochrom.co.uk Accounts accounts@biochrom.co.uk +44 (0) 1223 427816 Biochrom EZ Read 400 User’s Manual Version 2.1... -
Page 20: Appendix
Use optically clear microplates with flat wells for the best results. Other microplates can be used as well: consult the Biochrom website for a list of microplates that are approved for use with the Biochrom EZ Read 400 Microplate Reader. -
Page 21: Quick Start Guide
Quick Start Guide Print this guide and keep in the lab with the Biochrom EZ Read 400 Microplate Reader for easy reference. To turn on the instrument: Connect instrument to a power source using the appropriate power cord. Turn on the instrument using the switch at the back of the instrument. - Page 22 ADAP. When prompted, enter a plate ID. Data can be exported to data analysis software using the Copy icon. Data will paste as a matrix with filter wavelength, with time and date. Biochrom EZ Read 400 User’s Manual Version 2.1...
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Page 23: Troubleshooting And Frequently Asked Questions
Below is a list of frequently asked questions and answers: What is the indication range of the EZ Read 400 and how does this specification differ from the linear range? The Biochrom EZ Read 400 has an indication range of 0.000-3.300 OD and a linear range of 0.100-2.500 OD. - Page 24 Set the instrument type to that of your instrument. g. Click on ‘Read Configuration’ button h. If the serial number and type values below the button are filled with numbers of the instrument, communication is successful Biochrom EZ Read 400 User’s Manual Version 2.1...
- Page 25 I can’t get the plate back. How can I return the plate transporter to its original position? Go to Setup>Instrument and select the Functions 1 tab. Select initialize and wait a few minutes for the plate transporter to return to the home position Biochrom EZ Read 400 User’s Manual Version 2.1...
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