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GeneQuant
QUICK REFERENCE GUIDE
TM
Biochrom US
84 October Hill Road
Holliston, MA
01746-1388
USA
1300
Telephone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
support@hbiosci.com
www.biochromspectros.com

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Summary of Contents for biochrom GeneQuant 1300

  • Page 1 GeneQuant 1300 QUICK REFERENCE GUIDE Biochrom US Telephone: 1-508-893-8999 84 October Hill Road Toll Free: 1-800-272-2775 Holliston, MA Fax: 1-508-429-5732 01746-1388 support@hbiosci.com www.biochromspectros.com...
  • Page 2 The Instrument Alphanumeric keys Confirm selection/ Go to next screen Display screen Escape/cancel On/Off key Arrow keys Set reference View options Take measurement Action On/off key Turns the instrument on/off Arrow keys Use the four arrow keys to navigate around the display and select the required setting from the active (highlighted) option.
  • Page 3 The Display Screen Navigation Move between boxes using the up and down arrows. Enter parameters by: using the key pad numbers If the box contains the symbol , either type in a value or press the options key , and choose a parameter from the next screen.
  • Page 4 Parameter Dictionary Parameter Folder Sub-Folder Manual Description and options page Life Science Nucleic Acids - Oligo Enter the proportion of Adenine bases. Default is 10, range: 0 – 9999. Only an option when units are pmol/µl A260 Life Science Protein – Protein UV Enter coefficient 2 (for absorbance at 260 nm).
  • Page 5 Parameter Folder Sub-Folder Manual Description and options page Curve Fit Applications Standard curve Select the type of curve fit to be used. Life Science Protein – BCA Options: straight line regression (forces the Protein – Bradford line through the origin), zero regression, Protein –...
  • Page 6 Parameter Folder Sub-Folder Manual Description and options page Factor Life Science OD600 Enter the factor. Range 0 – 9999. Only available if units are cells/ml Folder Ulitilites Folder Names Select a folder to rename. Options: Methods 1-9 or Favourites Life Science Nucleic Acids - Oligo Enter the proportion of Guanine bases.
  • Page 7 Parameter Folder Sub-Folder Manual Description and options page Pathlength Applications Absorbance Ratio - Select the relevant path length – 5 or 10 Parameters Life science Nucleic Acids – DNA Nucleic Acids – RNA Nucleic acids – Oligo Tm Calculation Cy Dye Protein –...
  • Page 8 Parameter Folder Sub-Folder Manual Description and options page Units Applications Concentration Enter the units using the alphanumeric keys Kinetics – Parameters 2 or press and select pre-defined units Standard Curve using the left and right arrows (options: Life Science Protein – BCA (µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, Protein –...
  • Page 9 Parameter Folder Sub-Folder Manual Description and options page Y axis limits Applications Wavescan, options 6 - Set to On to define the start and finish Graph Scale points of the y axis, or off to retain default values Applications Wavescan, options 6 - Enter the minimum value for the y axis Graph Scale Applications...