biochrom Anthos Fluido 2 User Manual
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biochrom Anthos Fluido 2 User Manual

Microplate washer
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Biochrom Anthos Fluido 2
Biochrom Ltd
22 Cambridge Science Park,
Milton Rd, Cambridge CB4 0FJ UK
Tel: +44 (0)1223 423723
Fax: +44 (0)1223 420164
Email: enquiries@biochrom.co.uk
www.biochrom.co.uk
Biochrom Anthos Fluido-2 Microplate Washer v2.0
Microplate Washer
User's Manual
Biochrom Ltd
22 Cambridge Science Park,
Milton Rd, Cambridge CB4 0FJ UK
Tel: +44 (0)1223 423723
Fax: +44 (0)1223 420164
Email: enquiries@biochrom.co.uk
www.biochrom.co.uk
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  • Page 1 Milton Rd, Cambridge CB4 0FJ UK Milton Rd, Cambridge CB4 0FJ UK Tel: +44 (0)1223 423723 Tel: +44 (0)1223 423723 Fax: +44 (0)1223 420164 Fax: +44 (0)1223 420164 Email: enquiries@biochrom.co.uk Email: enquiries@biochrom.co.uk www.biochrom.co.uk www.biochrom.co.uk Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 1...

  • Page 2: Table Of Contents

    Run Procedure ............................25 7.3.1 Prime / Rinse ............................26 7.3.2 Define Procedure ............................ 27 7.3.3 Define Cycle ............................28 7.3.4 Define Plate ............................29 7.3.5 Setup ..............................30 7.3.6 Service ..............................31 7.3.7 Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 2...
  • Page 3 11.1 Approved parts ............................73 11.2 Exchanging a Fuse ............................73 11.3 Cleaning the instrument ..........................74 11.4 Disinfection Procedure ..........................74 11.5 Intended Use ....................76 Quality control ............................76 12.1 Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 3...

  • Page 4: Introduction

    The Fluido 2 can be operated with up to 3 different wash liquids to be selected by software. An easy plate setup is provided to optimise the movement of the plate and the manifold according to the different dimensions of different plate types. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 4...

  • Page 5: Warnings And Hazards

    Any adjustment or repair of the opened apparatus under voltage should be avoided, but, if necessary, it must be carried out by qualified service personnel who are aware of the hazards involved. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 5...

  • Page 6: Safety Symbols

    The following safety symbols may be found in several locations on the instrument. Only persons who fully understand the safety precautions and recognize shock hazards should operate this instrument. Alternating current In Vitro Diagnostic Device Protective ground terminal Caution, see enclosed document Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 6...
  • Page 7 The symbol on the product indicates that this product shall not be treated as household waste. Instead it shall be handed over to the applicable collection point for the recycling of electrical and electronic equipment Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 7...

  • Page 8: Precautions

    The usual wash buffer tends to crystallize easily on air and can then block tubing and fittings. Use the automatic rinse program daily and do not leave the tubing filled with wash buffer for an extended period of time. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 8...

  • Page 9: Specifications

    The tolerances of the measuring devices are based on the manufacturer‟s information. General technical data 4.2.1 Dimensions Weight: 6,2 kg net Dimensions (width x length x height): 21 cm x 46 cm x 21 cm 4.2.2 Power supply Consumption: 80VA (maximum) Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 9...

  • Page 10: Interfacing

    General features The Fluido 2 incorporates state of the art technology and software both necessary to allow optimum performance and easy use. The Fluido 2 is designed around your application. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 10...
  • Page 11  Bottom Detection As part of the plate setup this unique feature supports optimization of plate specific parameters. The instrument is able to automatically detect the Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 11...

  • Page 12: Standard Delivery Content

    Standard delivery content Washer Fluido 2 external tubing set Manifold (8-way) 1 or 3 wash bottles 2 L (depending on configuration) Rinse bottle 2 L Waste bottle 2.5 L Power cord Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 12...

  • Page 13: Warnings And Directions

    Do not touch the plate during movement of the plate transport (risk of injury). The equipment may not be opened other than as described in the chapter 11 Maintenance, since it contains live parts (danger to life). Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 13...

  • Page 14: Unpacking Procedure

    Only use the power cable supplied with the instrument or a power cable with protective earth connection carrying the CE-mark. The power outlet the instrument is connected to has to meet the applicable technical safety requirements! Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 14...

  • Page 15: Instrument Installation Procedure

    When connecting the instrument with an external computer make sure the instrument is switched off (the power indicator is dark). If the instrument is turned on turn it off using the power switch on the rear side of the device. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 15...
  • Page 16 In order to make sure the instrument is disconnected from power the main switch on the rear side of the unit has to be switched to the "OFF-position" indicated with a "0" symbol. Alternatively the power cord can be disconnected from the unit. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 16...

  • Page 17: Re-Packing To Prevent Damage During Transport

    It is therefore recommended to save the original carton with its foam parts and accessories box. Warranty claims are void if transport damages are caused by improper packing! picture 1 picture 2 picture 3 Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 17...

  • Page 18: Theory Of Operation

    Any kind of container with sufficient volume can be used for the waste. In addition the risk of infectious aerosols is much reduced. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 18...
  • Page 19 Diagram 1. Hydraulic Diagram Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 19...

  • Page 20: Warranty

    Liability In original condition the instrument meets all safety regulations for a risk- less operation. Anthos cannot warrant damages or any resulting costs caused by alterations, repairs or modifications of the equipment. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 20...

  • Page 21: Software Principle

    (e.g. "Proc1" for the procedure with this name). To change the selection use the <+> and <-> keys. These keys can also be used for numerical or alphanumerical inputs by means of in- or decreasing a value or scrolling through the alphabet. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 21...
  • Page 22 By pressing <enter> the current selection is confirmed and the software leads to the next menu to allow further selections. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 22...

  • Page 23: Programming Sequence

    Detailed information for all menus and their functions can be found in the following chapters. Run Procedure: For details please refer to chapter 9.1 Run Procedure << >> Proc1 Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 23...
  • Page 24 For details please refer to chapter 9.5 Define Plate Menu << >> <new> Setup For details please refer to chapter 9.6 Setup Menu << >> Service For details please refer to chapter 9.7 Service Menu << >> Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 24...

  • Page 25: Run Procedure

    <-| if disabled W ashliquid OK? if disabled W aste em pty? Plate inserted? if disabled Perform Stop Procedure Table 4. Run Procedure For details please refer to chapter 9.1 Run Procedure Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 25...

  • Page 26: Prime / Rinse

    <-| prime/rinse Automatic Volume Automat <-| Liquid <-| Rinse Stop Rinse Period <-| Liquid <-| Volume <-| Periodic Rinse interval Table 5. Prime / Rinse For details please refer to chapter 9.2 Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 26...

  • Page 27: Define Procedure

    A N D 'F in a l a sp tim e ' > 0 < -| S to rin g Table 6. Define Procedure For details please refer to chapter 9.3 Define Procedure Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 27...

  • Page 28: Define Cycle

    < < Min im u m so a k tim e E sc < -| S to rin g Table 7. Define Cycle For details please refer to chapter 9.4 Define Cycle Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 28...

  • Page 29: Define Plate

    B o tto m d isp h t < -| O vflo w d isp h t E sc < -| S to rin g Table 8. Define Plate For details please refer to chapter 9.5 Define Plate Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 29...

  • Page 30: Setup

    < < Pla te wa rn in g < -| < < Ke y click < -| Sto rin g Table 9. Setup For details please refer to chapter 9.6 Setup Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 30...

  • Page 31: Service

    7.3.7 Service << Service >> <-| Sensors Valves Pumps PlatInit PlatPos MFInit MFPos MFAdjust PlAdjust Eeprom DelParam Table 10. Service For details please refer to chapter 9.7 Service Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 31...

  • Page 32: Description Of The Key Functions

    <+> / <-> - These keys are used for numerical or text input and to select options from a list (e.g. selection of the procedure to be started). <enter> - confirms the text / numerical entry or selection and proceeds to the next dialogue element. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 32...

  • Page 33: Start-Up And System Setup

    Proc1 - current selection (or field for text or numerical input); use the <-> and <+> keys to alter a selection or Setup << >> change a value Control of the system setup Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 33...
  • Page 34 Please make sure to set all options according to your requirements before programming new procedures, plates or cycles and before running procedures. For details on this menu please refer to chapter 9.6 Setup Menu Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 34...

  • Page 35: Programming

    A warning to check the liquid level of the wash bottle is displayed (this function needs to be activated in the "Setup Menu").  Function keys: <F1> or <F2> Select "Yes" or "No". If "No" is selected the instrument will return to the main menu. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 35...
  • Page 36 The display prompts you to enter the right numbers of stripes (this function needs to be activated in the "Setup Menu").  Function keys: <F1> Select Esc: the instrument will return to the main menu <->/<+> Select number of stripes Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 36...
  • Page 37 <enter> Confirms the selection and starts procedure After confirmation of the above warnings the selected procedure will be started. During the run the display will show the currently performed cycles. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 37...

  • Page 38: Prime/Rinse Menu

    <F1> or <F2> Select "Esc" to return to the main menu. Manual pri/rinse <+> or <-> Select the desired liquid. <enter> Confirm the selection of the liquid and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 38...

  • Page 39: Automatic Prime/Rinse

    <+> or <-> Select the desired liquid. <enter> Confirm the selection of the liquid and go to the next screen. Screen 22. Volume Selection Select the liquid volume for prime / rinse. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 39...

  • Page 40: Periodic Prime/Rinse

    Select "Stop" to cancel the current prime / rinse cycle and return to the main menu. 9.2.3 Periodic Prime/Rinse Screen 25. Liquid Selection Select the liquid to be used for prime / rinse. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 40...
  • Page 41 Select "Esc" to return to the main menu. <+> or <-> Select the desired time period in steps of 1min (max. 200min). <enter> Confirm the selection of the time and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 41...

  • Page 42: Define Procedure Menu

    The cycles and the plate must be defined prior to the procedure.  Function keys: <F1> or <F2> Select another menu. <+> or <-> Select "<new>" to define a new procedure or select an existing procedure to edit it. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 42...
  • Page 43 <F1> or <F2> Select "Esc" to return to the main menu without saving any changes or "<<" to return to the previous screen. <+> or <-> Use these keys to enter the wash liquid. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 43...
  • Page 44 "<<" to return to the previous screen. <+> or <-> Use these keys to scroll through the available cycles. Final asp time: << 0 sec <enter> Confirm the selection of the cycle and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 44...
  • Page 45 Select the processing mode to be used for this procedure.  Function keys: <F1> or <F2> Select "Esc" to return to the main menu without saving any changes or "<<" to return to the previous screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 45...
  • Page 46 Use these keys to toggle between "Yes" and "No". If "Yes" is selected you can define the last strip every time the procedure is started. "No" means that every time all strips of a plate are washed. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 46...

  • Page 47: Define Cycle Menu

    Select another menu. <+> or <-> Select "<new>" to define a new cycle or select an existing cycle to edit <enter> Confirm the selection of the cycle and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 47...
  • Page 48 Use these keys to change the aspiration time (the time the manifold remains inside a well during aspiration) in steps of 1sec (range: 0 - 10 seconds). <enter> Confirm the selection of the aspiration time and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 48...
  • Page 49 Use these keys to change the dispense volume for this cycle in steps of 50µl (range 0 - 2000µl). <enter> Confirm the selection of the volume and go to the next screen. Disp power: << Esc High Screen 48. Dispenser Power Selection Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 49...
  • Page 50 Select "Esc" to return to the main menu without saving any changes or "<<" to return to the previous screen. <+> or <-> Use these keys to toggle between "Bottom" and "Overflow". Screen 50. Shaking Time Entry Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 50...
  • Page 51 Select "esc" to return to the main menu without saving any changes or "<<" to return to the previous screen. <+> or <-> Use these keys to change the minimum soaking time in steps of 10sec (range: 0 - 1000 seconds). Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 51...

  • Page 52: Define Plate Menu

    The following drawings help to find the correct positions of the manifold and plate. Please note that curved bottom plates do not allow crosswise aspiration! Dispense needle Aspiration needle Dispense needle Aspiration needle Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 52...
  • Page 53 Aspiration needle Aspiration needle Vertical 0-Position Vertical 0-Position Ovflow disp ht Bottom disp ht Horizontal 0-Position Disp pos Horizontal 0-position Disp pos Drawing 54. Wash Positions for flat bottom and curved bottom plates Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 53...
  • Page 54 "Sel" to select the current letter/number. <+> or <-> Use these keys to scroll through the available letters (A-Z) and numbers (0-9) <enter> Confirm the name of the plate and go to the next screen. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 54...
  • Page 55 The center position may be defined by "teach-in" (move the plate to the correct position with the <+> and <-> keys). Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 55...
  • Page 56 (side pos rear). There the needle is moved down and another aspiration sequence takes place. For a better aspiration result crosswise Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 56...
  • Page 57 Crosswise aspiration can only be used for flat- bottom plates. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 57...
  • Page 58 In this "teach-in" you can move the plate to the correct position with the <+> and <-> keys.  Function keys: <F1> or <F2> Select "" to automatically detect the bottom of the well (changes to Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 58...
  • Page 59 In this "teach-in" mode the plate may be moved to the correct position with the <+> and <-> keys.  Function keys: <F1> or <F2> Select "" to automatically detect the bottom of the well (changes to “”). Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 59...
  • Page 60 Select "" to move the manifold up. Select “Mov” to move the plate transport to the current side position rear. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 60...
  • Page 61 Select "" to automatically detect the bottom of the well. <+> or <-> Use these keys to move the manifold. The manifold can be moved in steps of ~0.1mm (range: 0 – 18.8mm). Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 61...
  • Page 62 <+> and <-> keys. With “Mov” and “” check if the needles are in correct position.  Function keys: <F1> or <F2> Select "" to automatically detect the bottom of the well (changes to “”). Select "" to move the manifold up. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 62...
  • Page 63 In this "teach-in" mode you can move the manifold to the correct height with the <+> and <-> keys.  Function keys: <F1> or <F2> Select "" to automatically detect the bottom of the well. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 63...
  • Page 64 In this "teach-in" mode you can move the manifold to the correct height with the <+> and <-> keys.  Function keys: <F1> or <F2> Select "" to automatically detect the bottom of the well. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 64...

  • Page 65: Setup Menu

    Use the <-> / <+> keys to select the language and confirm with <enter>. Manifold: << 8needle Screen 77. Manifold Setup Use the <-> / <+> keys to select the manifold and confirm with <enter>. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 65...
  • Page 66 Use the <-> / <+> keys to select the number of wash liquids connected to your instrument and confirm with <enter>. Depending on the hardware configuration of your instrument you can use up to 3 wash liquids . Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 66...
  • Page 67 In case that the level of the Wash2 empty det: << liquid in the wash1 bottle is below the sensor a corresponding warning will be displayed. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 67...
  • Page 68 Use the <-> / <+> keys to activate/deactivate the liquid warning and confirm with <enter>. If activated a reminder to check the level of the wash bottle(s) is displayed prior to the start of a procedure. Screen 85. Waste Warning Setup Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 68...

  • Page 69: Service Menu

    Service << >> Sensors Service Menu Screen 89.Service Menu This menu allows to check various functions of the instrument. It is only intended to be used by trained service personnel. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 69...

  • Page 70: Error Messages

    Confirm the message. 10.1.3 No Cycle Defined No cycle defined Press any key Screen 92.No Cycle Defined There is no cycle defined.  Function keys: <enter> Confirm the message. 10.1.4 No Plate Defined Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 70...

  • Page 71: Waste Full

     Function keys: <enter> Confirm the message. Liquid empty Press any key 10.1.6 Liquid Empty Screen 95. Liquid Empty The selected wash liquid is empty.  Function keys: <enter> Confirm the message. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 71...

  • Page 72: Stop Pressed

    The plate transport failed.  Function keys: <enter> Confirm the message. MF-trans error Press any key 10.1.9 Manifold Transport Error Screen 98.Manifold Transport Error The manifold transport failed.  Function keys: <enter> Confirm the message. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 72...

  • Page 73: Maintenance

     Disconnect the instrument from mains by unplugging the power cable.  Open fuse-carrier next to the mains socket with a screwdriver  Remove fuse  Insert spare fuse included in supply (2 pcs. 2.5 A TH250VAC Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 73...

  • Page 74: Cleaning The Instrument

    2. Carefully wipe all the outside surfaces of the instrument and the plate area with a wad of cotton wool that has been soaked in the disinfection solution. Note: Ensure that disposable gloves are worn. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 74...
  • Page 75 The pumps can be disinfected by dispensing and aspirating a sufficient amount of disinfectant fluid. After the disinfection procedure dispense > 200 ml of distilled water to remove all disinfectant fluid from the liquid carrying parts. Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 75...

  • Page 76: Intended Use

    For the performance of any reagent quality control it has to be referred exclusively to those instructions. It is the user's responsibility to ensure proper operation according to the instructions given with each individual test! Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 76...

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