Page 1 , Utilities & Accessories Aquameter ® firmware Version 6.20 and Above AP-5000 firmware Version 4.07 and Above Document No. 10401-00906 Revision: M Date: August 20, 2019 Aquaread ® Bridge House Northdown Industrial Park Broadstairs Kent CT10 3JP ENGLAND Phone: +44 1843 600 030...
Page 2 Notwithstanding the foregoing, in no event shall Aquaread ® Ltd and/or its affiliates aggregate liability arising under or in connection with Aquaread ® products, regardless of the number of events, occurrences, or claims giving rise to liability, be in excess of the price paid by the purchaser for the Aquaread ®...
Page 3: Table Of Contents
To Check / Calibrate the 100% Saturation Point in Damp Air................28 10.4. C ...........................29 ALIBRATION RROR ESSAGES 10.5. R ......................30 ESETTING TO ACTORY ALIBRATION EFAULTS 10.6. C ..............................30 ALIBRATION EPORTS 10.7. C .......................30 ALIBRATION TORAGE AND ETRIEVAL 11. AFTER USE..................................32 © 2019 Aquaread Ltd. www.aquaread.com Page 3 of 138 ®...
Page 4 15.5.9. Errors During Calibration..........................60 15.5.10. Lens and Sleeve Maintenance........................60 15.6. 2000/5000-BGA-PE S ...........61 ALTWATER REEN LGAE PHYCOERYTHRIN LECTRODE 15.6.1. Principle of Operation...........................61 15.6.2. Limitations of Use............................61 15.6.3. Calibrating the BGA-PE Electrode........................61 © 2019 Aquaread Ltd. www.aquaread.com Page 4 of 138 ®...
Page 5 15.11.3. Calibrating the CDOM Electrode........................86 15.11.4. Calibration Solutions...........................86 15.11.5. Zero Point Calibration..........................86 15.11.6. Calibrating Point 2............................88 15.11.7. Calculating and Applying a Grab Sample Factor..................88 15.11.8. Errors During Calibration...........................89 15.11.9. Lens and Sleeve Maintenance........................89 © 2019 Aquaread Ltd. www.aquaread.com Page 5 of 138 ®...
Page 6 17.10. E ..............................113 XPORTING 17.11. E ............................113 XPORTING EPORTS 17.12. T ........................115 YPICAL EPORT OVER 17.13. E ® F ............................116 XPORTING XCEL ILES 17.14. E ™ F ...........................116 XPORTING OOGLE ILES © 2019 Aquaread Ltd. www.aquaread.com Page 6 of 138 ®...
Page 7 RIOR ETURN 18.2. D ..........................119 ECONTAMINATION ERTIFICATE 19. TROUBLESHOOTING.............................120 20. DECLARATION OF CONFORMITY........................121 21. APPENDIX 1. THE TECH BEHIND AQUAREAD’S OPTICAL DO MEASUREMENT SYSTEM....122 21.1. P ............................122 RINCIPLE OF PERATION 21.2. S ..............................123 ENSOR 22. APPENDIX 2. INSTALLING AUX ELECTRODES.....................124 22.1.
Page 8: Introduction
Aquameter ® are running earlier firmware, the functionality may differ from that shown in this manual. In this case, contact Aquaread for an earlier version of this manual or return your equipment for firmware upgrade. 2. What’s in the Box? The Aquameter ®...
Page 9: About The Probe Sleeve And Sleeve End Cap
Aquaprobe undamaged. Sacrificial anodes are, as the name suggest, sacrificial, so will need replacing periodically. Sacrificial anodes are available for all models of Aquaprobes. Please contact Aquaread Ltd for more details. 2.4. About the Probe Sleeve and Sleeve End Cap The AP-5000 is constructed with an aluminium sleeve surrounding the delicate sensing electrodes.
Page 10: Top Tips For Successful Measurements Using Optical Electrodes
➢ Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. This is especially important when using the Turbidity electrode. © 2019 Aquaread Ltd. www.aquaread.com Page 10 of 138 ®...
Page 11: Battery Installation And Care
After a further 15 seconds the backlight will switch off. During normal operation, if you want to activate the backlight without changing the Meter function, simply press the ESC key. © 2019 Aquaread Ltd. www.aquaread.com Page 11 of 138...
Page 12: Overview Of The Operating System
When the time and date are correct, press the OK key. Don’t worry if you make a mistake first time round. You can easily get back to these screens later through the MENU key. © 2019 Aquaread Ltd. www.aquaread.com Page 12 of 138 ®...
Page 13: Connecting An Ap-5000
To connect the Extension Cable to the AP-5000, align the coloured dot on the AP-5000 with the ◄AQUAREAD logo on the plug body, then press the plug into the socket and tighten the retaining collar fully. Once the AP-5000 has been connected to the Extension Cable, the Aquameter ®...
Page 14: Taking Measurements
EC:6541µS/cm   TDS:3271mg/L GPS:3D Pos   SAL:03.57 PSU    SSG:01.3σt  GPS:3D Pos    BARO:1013mb DEPTH:01.75m Hit [OK] to zero GPS:3D Pos   © 2019 Aquaread Ltd. www.aquaread.com Page 14 of 138 ®...
Page 15: What Does It All Mean
In addition to the individual trend indications, there is a global stability indication, which is displayed when all readings are stable. This takes the form of a flashing double headed arrow which is displayed at the start of the third line of the display. © 2019 Aquaread Ltd. www.aquaread.com Page 15 of 138 ®...
Page 16: Temperature Compensation
(usually 20ºC or 25ºC) although a variation of up to +/-10ºC makes very little difference in reality. During pH measurement, temperature variation is automatically compensated for. © 2019 Aquaread Ltd. www.aquaread.com Page 16 of 138 ®...
Page 17: Gps Reception
® (AM-200) contains a built-in GPS/GLONAS receiver and antenna. The antenna is situated at the top of the case, just behind the AQUAREAD Logo. For optimum signal reception, the antenna must be able to ‘see’ a reasonably large amount of the sky. The GPS receiver will not work indoors or when shielded from the sky by any solid structure.
Page 18: Depth Measurement
Probe. If two point depth calibration is required, it must be done using an AP-PC KIT and associated AquaCal PC Software. Full depth calibration can not be performed using an Aquameter ® © 2019 Aquaread Ltd. www.aquaread.com Page 18 of 138...
Page 19: Memory Mode
Using the up/down keys, select the electrode you are interested in, then press either the OK key or the right arrow key. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. © 2019 Aquaread Ltd. www.aquaread.com Page 19 of 138...
Page 20: Clearing The Memory
Please note, the first item on the menu, 'Clean Probe', will only be active if an Aquaprobe ® AP-7000 (which has an automatic cleaning system) is connected. Clean Probe  Auto Data Logging Calibration Setup & Install © 2019 Aquaread Ltd. www.aquaread.com Page 20 of 138 ®...
Page 21: Battery And Memory Duration In Low Power Logging Mode
The battery life figures quoted below are based on fresh, good quality alkaline batteries at a Meter temperature of 21°C or over. Colder Meter temperatures will drastically reduce the battery life. For example, at 5°C, the battery life will be approximately half that quoted. © 2019 Aquaread Ltd. www.aquaread.com Page 21 of 138 ®...
Page 22: Important Information About Memory Mode
The same rules apply when data is output to a PC running AquaLink Software via the USB cable. The data that is output is always as per the Meter’s current configuration. You can output the data as many times as you like in various Meter configurations. © 2019 Aquaread Ltd. www.aquaread.com Page 22 of 138 ®...
Page 23: Setup & Install
Finally, this screen allows you to set the factor that the Meter uses to calculate Total Dissolved Solids from Specific EC. This is the TDS Fact: (TDS = EC x TDS Fact) and can be set anywhere between 0.00 and 1.00. Default value is 0.65. © 2019 Aquaread Ltd. www.aquaread.com Page 23 of 138 ®...
Page 24: Aux Socket Assignment
The Socket Assignment option is only available if the Aquameter ® is connected to an AP- 5000. This is because the assignment data is held in the Aquaprobe ® , not in the Aquameter ® © 2019 Aquaread Ltd. www.aquaread.com Page 24 of 138 ®...
Page 25 Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. If you subsequently remove an electrode, be sure to set the socket assignment back to EMPTY. © 2019 Aquaread Ltd. www.aquaread.com Page 25 of 138...
Page 26: Rapidcal Calibration Method
Probe is to be used. In addition, you should check the DO 100% calibration point and zero any optical electrodes you may have fitted. To use RapidCal: © 2019 Aquaread Ltd. www.aquaread.com Page 26 of 138 ®...
Page 27 If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Calibrating 100% Press [OK] When calibration is complete, press OK then ESC to return to normal reading mode. © 2019 Aquaread Ltd. www.aquaread.com Page 27 of 138 ®...
Page 28: To Check / Calibrate The 100% Saturation Point In Damp Air
Finally, if you have any optical electrodes fitted, you should calibrate the zero points now using fresh, still mineral water. Refer to the relevant sections in this manual for advice on zero point calibration of your optical electrodes. © 2019 Aquaread Ltd. www.aquaread.com Page 28 of 138 ®...
Page 29: Calibration Error Messages
Probe’s optical and EC measurement system, and MUST be fitted during calibration and measurement for correct operation. If you try to calibrate the Probe without the sleeve fitted, you may get an error message. © 2019 Aquaread Ltd. www.aquaread.com Page 29 of 138...
Page 30: Resetting To Factory Calibration Defaults
Meter, this data is transferred for display and logging. This is a major advantage and allows you to use a variety of different Probes with a single Meter, without the need for re-calibration. © 2019 Aquaread Ltd. www.aquaread.com Page 30 of 138...
Page 31 Pushing the left or right arrow keys will toggle this screen with the calibration report screen. PH7 Offset:+01.2mV PH4 Slope:56.8mV/pH PH10 Slope:56.3mV/pH ORP Offset:-02.6mV The calibration report screen displays the calibration report values from the last calibration. To exit this screen press the ESC key. © 2019 Aquaread Ltd. www.aquaread.com Page 31 of 138 ®...
Page 32: After Use
TIP: Occasional application of a smear of silicone grease or similar lubricant to the connector O-rings and thread, Sleeve thread, the protective Sleeve End Cap thread will make fitting and removal of these parts easier. © 2019 Aquaread Ltd. www.aquaread.com Page 32 of 138 ®...
Page 33: General Probe Maintenance
The photograph below shows the two standard electrodes, the Combi pH/ORP electrode and the DO/EC electrode. The DO/EC electrode includes the Optical DO sensor the EC sensor and the temperature sensor. These electrodes will be referred to in the next two sections. © 2019 Aquaread Ltd. www.aquaread.com Page 33 of 138...
Page 34: Ph/Orp Electrode Calibration And Maintenance
Electrode Storage Solution each time it is removed and replaced. If a pH/ORP electrode is inadvertently allowed to dry out, it must be re-hydrated by soaking in storage solution for at least one hour prior to use. © 2019 Aquaread Ltd. www.aquaread.com Page 34 of 138 ®...
Page 35: Calibrating Ph
4. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF – 104ºF). 5. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2019 Aquaread Ltd. www.aquaread.com Page 35 of 138 ®...
Page 36 Press OK then ESC repeatedly to return to normal reading mode. Remove the Probe from the calibration cup, rinse thoroughly in de-ionised water, shake off any excess and dry the outer sleeve with a soft cloth. © 2019 Aquaread Ltd. www.aquaread.com Page 36 of 138...
Page 37: Calibrating The Second Point
13.8. Servicing the pH Electrode Remove the pH or combined pH/ORP electrode from the Probe body (see Electrode Removal and Replacement). Rinse with methyl alcohol. Replace the electrode. Re-calibrate. © 2019 Aquaread Ltd. www.aquaread.com Page 37 of 138 ®...
Page 38: Calibrating Orp
Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2019 Aquaread Ltd. www.aquaread.com Page 38 of 138 ®...
Page 39 Remove the Probe from the calibration cup, rinse thoroughly in fresh water, shake off any excess and dry the outer sleeve with a soft cloth. Dampen the sponge in the storage cap with storage solution and fit it to the pH/ORP electrode. ORP calibration is now complete. © 2019 Aquaread Ltd. www.aquaread.com Page 39 of 138 ®...
Page 40: Converting Orp Readings To The Hydrogen Scale
The ORP reference electrode used in Aquaread ® combination electrodes is a 3MPK1 silver...
Page 41: Do/Ec Electrode Calibration And Maintenance
The AP-5000 features an optical DO sensor. This sensor does not use a liquid electrolyte and has a black rubber gas-permeable membrane. See Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System for further details. 14.3. Precautions During Use EC measurement is not possible with the Probe's Sleeve removed as the Sleeve forms an integral part of the measurement system.
Page 42 This value is stored in the Probe's memory and can be recalled at any time. See section 10.7. Calibration Data Storage and Retrieval Press OK then ESC repeatedly to return to normal reading mode. © 2019 Aquaread Ltd. www.aquaread.com Page 42 of 138...
Page 43: Calibrating The Do 100% Saturation Point In Moist Air
2. Unscrew the Optical DO Cap from the end of the DO/EC electrode by rotating it anti- clockwise. Do not touch the exposed optical components. 3. Apply a light smear of silicone grease to the thread and O ring. © 2019 Aquaread Ltd. www.aquaread.com Page 43 of 138...
Page 44 5. Carry out both Zero point and 100% point DO calibration as described earlier. Please Note: It is essential when replacing the Optical DO Cap to calibrate the Zero point BEFORE calibrating the 100% point. © 2019 Aquaread Ltd. www.aquaread.com Page 44 of 138 ®...
Page 45: Calibrating Ec
® dealers or can be easily manufactured by adding 33.03 grams of laboratory grade sodium chloride (99.9% pure) to 1 litre of deionised water. RapidCal is available from all Aquaread ® dealers. The Probe sleeve forms an integral, working part of the Probe’s EC measurement system, and MUST be fitted during calibration and measurement for correct operation.
Page 46 The screen will change to: PLEASE WAIT Stabilising 000% If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Cell constant:0.98 Calibrating 100% Press [OK] © 2019 Aquaread Ltd. www.aquaread.com Page 46 of 138 ®...
Page 47: Verifying Ec Calibration
DO/EC electrode with a soft cloth or toothbrush and non-abrasive detergent. Never use solvent or alcohol based products to clean the DO/EC electrode. After cleaning, replace the Probe sleeve and re-calibrate. © 2019 Aquaread Ltd. www.aquaread.com Page 47 of 138...
Page 48: Calibrating Temperature
If the offset has been correctly input, the Aquameter ® will now be reading the corrected temperature. The temperature correction offset is stored in the Aquaprobe ® and applied at all times going forward. © 2019 Aquaread Ltd. www.aquaread.com Page 48 of 138 ®...
Page 49: Optional Optical Electrodes Calibration And Maintenance
➢ Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. This is especially important when using the Turbidity electrode. © 2019 Aquaread Ltd. www.aquaread.com Page 49 of 138 ®...
Page 50: Optical Electrode Calibration Sequence
If you attempt to calibrate an upper calibration point without first calibrating the ZERO point, a calibration error will occur. 15.3. Fluorescent Electrode Grab Sample Correction Factor A unique feature of the Aquaread ® fluorescent type electrodes is the ability to include a correction factor based upon a grab sample.
Page 51: 2000/5000-Turb Turbidity Electrode
Sleeve End Cap fitted. If you experience any problems calibrating or using the Turbidity Electrode, refer to Appendix 7. Troubleshooting Turbidity © 2019 Aquaread Ltd. www.aquaread.com Page 51 of 138...
Page 52: Negative Turbidity Readings
A three point calibration should be carried out once a month to ensure optimum accuracy. Zeroing of the Turbidity electrode is normally carried out automatically during RapidCal (see RapidCal Calibration Method). © 2019 Aquaread Ltd. www.aquaread.com Page 52 of 138 ®...
Page 53: Turbidity Zero Point Calibration
OK or right arrow key to select TURB. The screen will change to: CALIBRATE TURB ZERO? [01/Jan/14]  1000? [01/Jan/14] [01/Jan/14] The dates shown to the right of each point are the dates of the last successful calibration. © 2019 Aquaread Ltd. www.aquaread.com Page 53 of 138 ®...
Page 54: Verifying The Zero Calibration
Turbidity Standard solution (available from most lab supply companies) several times to thoroughly mix. Formazin Turbidity Standard is hazardous to your health. Be sure to handle with care and to read and comply with all health and safety advice. © 2019 Aquaread Ltd. www.aquaread.com Page 54 of 138...
Page 55: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 55 of 138 ®...
Page 56: 2000/5000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2019 Aquaread Ltd. www.aquaread.com Page 56 of 138...
Page 57: Calibration Solution Preparation
100μg/L. This solution can now be used as Pt-2 calibration of the BGA-PC sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2019 Aquaread Ltd. www.aquaread.com Page 57 of 138...
Page 58: Zero Point Calibration
Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. Select ZERO. The screen will change to: © 2019 Aquaread Ltd. www.aquaread.com Page 58 of 138...
Page 59: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2019 Aquaread Ltd. www.aquaread.com Page 59 of 138 ®...
Page 60: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 60 of 138 ®...
Page 61: 2000/5000-Bga-Pe Saltwater Blue-Green Algae ( Phycoerythrin ) Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2019 Aquaread Ltd. www.aquaread.com Page 61 of 138...
Page 62: Calibration Solution Preparation
8μg/L. This solution can now be used as Pt-2 calibration of the BGA-PE sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2019 Aquaread Ltd. www.aquaread.com Page 62 of 138...
Page 63: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2019 Aquaread Ltd. www.aquaread.com Page 63 of 138 ®...
Page 64: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2019 Aquaread Ltd. www.aquaread.com Page 64 of 138 ®...
Page 65: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 65 of 138 ®...
Page 66: 2000/5000-Cphyll Chlorophyll Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2019 Aquaread Ltd. www.aquaread.com Page 66 of 138...
Page 67: Calibration Solution Preparation
500μg/L. This solution can now be used as Pt-2 calibration of the CPHYLL sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2019 Aquaread Ltd. www.aquaread.com Page 67 of 138...
Page 68: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2019 Aquaread Ltd. www.aquaread.com Page 68 of 138 ®...
Page 69: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2019 Aquaread Ltd. www.aquaread.com Page 69 of 138 ®...
Page 70: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 70 of 138 ®...
Page 71: 2000/5000-Rhod Rhodamine Electrode
This is exactly the same calibration solution that is recommended for calibration of the BGA-PC electrode. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Rhodamine WT standard is recommended: © 2019 Aquaread Ltd. www.aquaread.com Page 71 of 138...
Page 72: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2019 Aquaread Ltd. www.aquaread.com Page 72 of 138 ®...
Page 73 Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Output:2500mV Calibrating 100% Press [OK] © 2019 Aquaread Ltd. www.aquaread.com Page 73 of 138 ®...
Page 74: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 74 of 138 ®...
Page 75: 2000/5000-Fscein Fluorescein Electrode
In order to calibrate the FSCEIN electrode, a 100µg/L calibration solution of Fluorescein Dye should be used. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Fluorescein Dye is recommended: © 2019 Aquaread Ltd. www.aquaread.com Page 75 of 138...
Page 76: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2019 Aquaread Ltd. www.aquaread.com Page 76 of 138 ®...
Page 77 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7. Calibration Data Storage and Retrieval Press OK then ESC repeatedly to return to normal reading mode. © 2019 Aquaread Ltd. www.aquaread.com Page 77 of 138 ®...
Page 78: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 78 of 138 ®...
Page 79: 2000/5000-Refoil Refined Oil Electrode
Differences in the fluorescent response between various types of oil. • Differences in the fluorescent response caused by temperature. • Differences in the fluorescent response caused by ambient light. • Interference caused by turbidity. • © 2019 Aquaread Ltd. www.aquaread.com Page 79 of 138 ®...
Page 80: Special Precautions When Using The Refoil Electrode
Be sure to handle chemicals with care and to read and comply with all health and safety advice. 15.10.6. Serial Dilution 10ppm Napthalene salt can be prepared either as a one or two step process dependent upon the accuracy of the scales used. © 2019 Aquaread Ltd. www.aquaread.com Page 80 of 138 ®...
Page 81: Zero Point Calibration
4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2019 Aquaread Ltd. www.aquaread.com Page 81 of 138 ®...
Page 82: Calibrating Point 2
Pour 300mL of freshly mixed 1-5, naphthalenedisulfonic acid disodium salt calibration solution into a clean calibration cup then gently lower the Probe in all the way. © 2019 Aquaread Ltd. www.aquaread.com Page 82 of 138...
Page 83: Calculating And Applying A Grab Sample Factor
On a daily basis, the lenses on the electrode should be wiped over with a soft damp cloth. Similarly, the inside of the Probe Sleeve and Sleeve Cap should be kept clean and free from any deposits that may cause stray fluorescence. © 2019 Aquaread Ltd. www.aquaread.com Page 83 of 138 ®...
Page 84 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 84 of 138 ®...
Page 85: 2000/5000-Cdom/Fdom C
Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing concentrations. © 2019 Aquaread Ltd. www.aquaread.com Page 85 of 138...
Page 86: Calibrating The Cdom Electrode
In order to 'calibrate' the CDOM electrode, a 100ppb solution of Quinine Sulphate in sulphuric acid can be used. However, since Quinine Sulphate is extremely expensive and sulphuric acid is dangerous to handle, Aquaread Ltd has formulated an equivalent, non toxic standard for use during CDOM electrode calibration. This is available in 600mL bottles.
Page 87 Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. © 2019 Aquaread Ltd. www.aquaread.com Page 87 of 138 ®...
Page 88: Calibrating Point 2
This value should now be input on the bottom line of the electrode's calibration screen. Once the GS Factor value has been input, the OK key should be hit to send the Factor to the Probe. © 2019 Aquaread Ltd. www.aquaread.com Page 88 of 138 ®...
Page 89: Errors During Calibration
In order to avoid this, do not leave the Aquaprobe sitting in CDOM solution for more time than it takes to do the actual calibration. As soon as the Point 2 calibration is complete, remove the Aquaprobe from the CDOM calibration solution and rinse thoroughly. © 2019 Aquaread Ltd. www.aquaread.com Page 89 of 138...
Page 90: Optional Ise Electrodes Calibration And Maintenance
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2019 Aquaread Ltd. www.aquaread.com Page 90 of 138 ®...
Page 91: 5000/7000-Amm Ammonium/Ammonia Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2019 Aquaread Ltd. www.aquaread.com Page 91 of 138 ®...
Page 92 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: © 2019 Aquaread Ltd. www.aquaread.com Page 92 of 138 ®...
Page 93: Point 2
The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up as shown above. If the calibration is © 2019 Aquaread Ltd. www.aquaread.com Page 93 of 138 ®...
Page 94: Two-Point Calibration
1 only. 16.4.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2019 Aquaread Ltd. www.aquaread.com Page 94 of 138 ®...
Page 95: 5000/7000-Nit Nitrate Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2019 Aquaread Ltd. www.aquaread.com Page 95 of 138 ®...
Page 96 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2019 Aquaread Ltd. www.aquaread.com Page 96 of 138 ®...
Page 97: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2019 Aquaread Ltd. www.aquaread.com Page 97 of 138 ®...
Page 98: Two-Point Calibration
1 only. 16.5.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2019 Aquaread Ltd. www.aquaread.com Page 98 of 138 ®...
Page 99: 5000/7000-Chl Chloride Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2019 Aquaread Ltd. www.aquaread.com Page 99 of 138 ®...
Page 100 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2019 Aquaread Ltd. www.aquaread.com Page 100 of 138 ®...
Page 101: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2019 Aquaread Ltd. www.aquaread.com Page 101 of 138 ®...
Page 102: Two-Point Calibration
1 only. 16.6.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2019 Aquaread Ltd. www.aquaread.com Page 102 of 138 ®...
Page 103: 5000/7000-Cal Calcium Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2019 Aquaread Ltd. www.aquaread.com Page 103 of 138 ®...
Page 104 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2019 Aquaread Ltd. www.aquaread.com Page 104 of 138 ®...
Page 105: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2019 Aquaread Ltd. www.aquaread.com Page 105 of 138 ®...
Page 106: Two-Point Calibration
1 only. 16.7.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2019 Aquaread Ltd. www.aquaread.com Page 106 of 138 ®...
Page 107: 5000/7000-Flu Fluoride Electrode
10ºC cooler. In order to achieve this, one batch of the 0.5ppm solution should be put into a refrigerator and the other two solutions should be put into a water bath at 25ºC. © 2019 Aquaread Ltd. www.aquaread.com Page 107 of 138...
Page 108: Three-Point Calibration
Pt-2? [01/Jan/17] Pt-3? [01/Jan/17] Calibration point 1 (Pt-1) is the warm 0.5ppm point. Calibration point 2 (Pt-2) is the warm 5ppm point. Calibration point 3 (Pt-3) is the cool 0.5ppm point. © 2019 Aquaread Ltd. www.aquaread.com Page 108 of 138...
Page 109: Point 3
3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 5ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2019 Aquaread Ltd. www.aquaread.com Page 109 of 138 ®...
Page 110: Two-Point Calibration
1 only. 16.8.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2019 Aquaread Ltd. www.aquaread.com Page 110 of 138 ®...
Page 111: Aqualink Pc Software
Windows ® on a stand-alone PC with a minimum screen resolution of 1024 x 768 and an available USB 2.0 socket. 17.1. Downloading AquaLink PC Software from the Aquaread ® website ™ The AquaLink PC Software is available for download using the following link: ™...
Page 112: Uploading Data From Your Aquameter
On the right of the screen, the position at which the data was logged is displayed in the GPS boxes (when logged using an AM-200 GPS Aquameter ® only). Latitude and longitude © 2019 Aquaread Ltd. www.aquaread.com Page 112 of 138...
Page 113: O Nscreen Help
Once a set of logged data has been uploaded from the Aquameter ® , it can be saved on your PC as a Raw Data file. These files use a proprietary Aquaread ® format and are saved with a .amf (aquameter file) extension.
Page 114 , Microsoft ® WordPad is the preferred text editor for viewing AquaLink Text Reports as this handles text file formatting better than Microsoft ® Notepad. A typical report cover page follows. © 2019 Aquaread Ltd. www.aquaread.com Page 114 of 138 ®...
Page 115: Typical Text Report Cover Page
Blocks of individual readings, laid out in chronological order, follow this cover page. The readings picked out on the cover page can be cross-referenced to the blocks of individual readings using the Tag numbers. © 2019 Aquaread Ltd. www.aquaread.com Page 115 of 138...
Page 116: Exporting Excel Files
Please note: Although you have downloaded the Google™ Earth application and are running it from your PC, you still need to be connected to the Internet in order for the application to access satellite images. A typical Google™ Earth image follows. © 2019 Aquaread Ltd. www.aquaread.com Page 116 of 138...
Page 117: Google™ Example
If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo. © 2019 Aquaread Ltd. www.aquaread.com Page 117 of 138 ®...
Page 118: Limited Warranty
Meter and Probe sockets, and damage caused by leaking batteries are not covered. If service is required, contact our Service Department directly by email in the first instance (service@aquaread.com). Report the model number, date of purchase, serial number and problem. You will be given a Returns Authorisation number by our Service Department.
Page 119: Decontamination Certificate
Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________ Decontamination Procedure: _______________________________________________________________________ _______________________________________________________________________________________________ Certified by (print name) : ___________________________________ Title: __________________________________ Date: ______________________________________ Signature: __________________________________ © 2019 Aquaread Ltd. www.aquaread.com Page 119 of 138 ®...
Page 120: Troubleshooting
This section details some of the common difficulties you may encounter when using the Aquameter ® , Aquaprobe ® and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@aquaread.com). Problem Cause / Remedy The Aquameter ® will not ...
Page 121: Declaration Of Conformity
 20. DECLARATION OF CONFORMITY Aquaread ® Ltd declares that the equipment described herein is in compliance with the essential requirements and other relevant provisions of Directives 2004/108/EC and 1999/5/EC. © 2019 Aquaread Ltd. www.aquaread.com Page 121 of 138 ®...
Page 122: Appendix 1. The Tech Behind Aquaread's Optical Do Measurement System
Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev M ® 21. Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System 21.1. Principle of Operation The Aquaread ® AquaPlus™ Optical DO measurement system works on the principle of Dynamic Luminescence Quenching. A gas-permeable chemical known as a luminophore is excited with short bursts of blue light, which causes molecules in the luminophore to emit red photons.
Page 123: Sensor Cap Life
The combination of low duty cycle and variable excitation brightness can stretch the useful life of a sensor cap as far as several years. © 2019 Aquaread Ltd. www.aquaread.com Page 123 of 138 ®...
Page 124: Appendix 2. Installing Aux Electrodes
ENSURE NO GREASE IS APPLIED TO THE GOLD CONTACTS. Using a clean cloth or tissue paper, polish the gold contacts ensuring they are completely clean. Carefully insert the electrode into the AUX socket and tighten firmly until the O-ring is completely compressed. © 2019 Aquaread Ltd. www.aquaread.com Page 124 of 138...
Page 125: Socket Assignment And Calibration
Keep the blanking plug in a safe place. If you subsequently remove an electrode, be sure to replace the blanking plug and set the socket assignment back to EMPTY. © 2019 Aquaread Ltd. www.aquaread.com Page 125 of 138...
Page 126: Appendix 3. Standard Electrodes Detailed Specification
For this reason, when using the standard pH/ORP electrode, the water under test must have a minimum EC (electrical conductivity) of 100µS/cm. Special low EC pH electrodes are available to special order. © 2019 Aquaread Ltd. www.aquaread.com Page 126 of 138 ®...
Page 127: Appendix 4. Optical Electrodes Detailed Specification And Faqs
Gas or Liquid Chromatography in a laboratory although i f grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen in order to improve the accuracy of future readings. © 2019 Aquaread Ltd. www.aquaread.com Page 127 of 138 ®...
Page 128: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why
2. MLR (Minimum Level of Repeatability) is the value below which optical electrode readings become generally unreliable and unrepeatable (unless taken under ideal conditions) due to interfering factors such as refraction from visible air bubbles and microscopic aeration. © 2019 Aquaread Ltd. www.aquaread.com Page 128 of 138 ®...
Page 129: What Is The Accuracy Of The Optical Electrodes
If grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen of each fluorescent type electrode in order to improve the accuracy of future readings. © 2019 Aquaread Ltd. www.aquaread.com Page 129 of 138...
Page 130: Appendix 4. Ise Electrodes Detailed Specification
All ion selective electrodes exhibit calibration drift over time. Drift should not be a major problem where the electrodes can be frequently calibrated. However, if the electrodes are to be used in long-term deployment studies, drift is almost certain to occur. © 2019 Aquaread Ltd. www.aquaread.com Page 130 of 138...
Page 131: Special Notes Concerning Ise Electrodes And Ph Buffers
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2019 Aquaread Ltd. www.aquaread.com Page 131 of 138 ®...
Page 132: Appendix 7. Troubleshooting Turbidity
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2019 Aquaread Ltd. www.aquaread.com Page 132 of 138 ®...
Page 133: About Turbidity Measurement
If you experience negative turbidity readings, thoroughly clean the Probe then re-zero in completely clean water. Still, bottled mineral water is recommended for zeroing the electrode as it is cheap and readily available. Never use sparkling or carbonated water. © 2019 Aquaread Ltd. www.aquaread.com Page 133 of 138 ®...
Page 134: Aeration
10mm to the probe sleeve. 26.7. Sensor Saturation Sensor saturation is a very rare problem that can occur in shallow water when combined with very bright sunlight. © 2019 Aquaread Ltd. www.aquaread.com Page 134 of 138 ®...
Page 135: Top Tips For Successful Measurements Using Optical Electrodes
➢ Always calibrate and zero the electrode as close to your sample temperature as possible. ➢ Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. © 2019 Aquaread Ltd. www.aquaread.com Page 135 of 138...
Page 136: References
10401-00906 Rev M ® 26.9. References This section is based upon Aquaread's experience in the field and information from the following sources. ➢ National Field Manual For the Collection of Water-Quality Data, Turbidity section 6.7, Revised by Chauncey W. Anderson, USGS, 2004.
Page 137: Appendix 8. Flow Through Cell
AP-5000. It is essential to re-calibrate the AP-5000's optical electrodes AFTER the Adapter Ring has been fitted to ensure correct performance. This is especially important for Turbidity measurement. © 2019 Aquaread Ltd. www.aquaread.com Page 137 of 138 ®...
Page 138: Ap-5000 Installation Into The Flowcell
✔ Aeration of sample water. Check all joints for air abnormally high or are leaks. Reduce flow rate to avoid cavitation. fluctuating wildly. ✔ Optical electrode not zeroed after adapter ring fitted. Zero the optical electrode. © 2019 Aquaread Ltd. www.aquaread.com Page 138 of 138 ®...

Aquaread Aquaprobe AP-5000 Instruction Manual

1 Introduction

2 What's in the Box

3 Battery Installation and Care

4 Overview of the Operating System

5 Connecting an Ap-5000

6 Taking Measurements

7 Depth Measurement

8 Memory Mode

9 Setup & Install

10 Rapidcal Calibration Method

11 After Use

12 General Probe Maintenance

13 Ph/Orp Electrode Calibration and Maintenance

14 Do/Ec Electrode Calibration and Maintenance

15 Optional Optical Electrodes Calibration and Maintenance

16 Optional Ise Electrodes Calibration and Maintenance

17 Aqualink Pc Software

18 Limited Warranty

19 Troubleshooting

20 Declaration of Conformity

21 Appendix 1. the Tech Behind Aquaread's Optical Do Measurement System

22 Appendix 2. Installing Aux Electrodes

23 Appendix 3. Standard Electrodes Detailed Specification

24 Appendix 4. Optical Electrodes Detailed Specification and Faqs

25 Appendix 4. Ise Electrodes Detailed Specification

26 Appendix 7. Troubleshooting Turbidity

27 Appendix 8. Flow through Cell

Quick Links

Troubleshooting

Need help?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Aquaread Aquaprobe AP-5000

Summary of Contents for Aquaread Aquaprobe AP-5000