Page 1 Aquaprobe ® firmware Revision 4.07 and Above Aquameter ® firmware Revision 6.20 and Above Document No. 10401-00870 Revision: R Date: March 27, 2017 Aquaread ® Bridge House Northdown Industrial Park Broadstairs Kent CT10 3JP ENGLAND Phone: +44 1843 600 030...
Page 2: Aquaprobe
Notwithstanding the foregoing, in no event shall Aquaread ® Ltd and/or its affiliates aggregate liability arising under or in connection with Aquaread ® products, regardless of the number of events, occurrences, or claims giving rise to liability, be in excess of the price paid by the purchaser for the Aquaread ®...
Page 3: What's New In This Manual
In order to have access to the new features, both the Aquaprobe ® and associated Aquameter ® must be upgraded together. Aquameters currently running firmware revisions lower than 4.50 can not be upgraded due to lack of sufficient memory space. © 2017 Aquaread ® Ltd. www.aquaread.com Page 3 of 141...
Page 4: Table Of Contents
To Check / Calibrate the 100% DO Saturation Point in Damp Air................30 10.4. C ...........................31 ALIBRATION RROR ESSAGES 10.5. R ......................31 ESETTING TO ACTORY ALIBRATION EFAULTS 10.6. C ..............................32 ALIBRATION EPORTS 10.7. C .......................32 ALIBRATION TORAGE AND ETRIEVAL 11. AFTER USE..................................34 © 2017 Aquaread ® Ltd. www.aquaread.com Page 4 of 141...
Page 5 PHYCOCYANIN LECTRODE 16.5.1. Principle of Operation...........................60 16.5.2. Limitations of Use............................60 16.5.3. Calibrating the BGA-PC Electrode........................60 16.5.4. Calibration Solution Preparation........................61 16.5.5. Serial Dilution..............................61 16.5.6. Zero Point Calibration...........................62 16.5.7. Calibrating Point 2............................63 © 2017 Aquaread ® Ltd. www.aquaread.com Page 5 of 141...
Page 6 16.10.10. Errors During Calibration.........................87 16.10.11. Lens and Sleeve Maintenance........................87 16.11. 2000/5000-CDOM/FDOM C .........88 HROMOPHORIC LUORESCENT ISSOLVED RGANIC ATTER 16.11.1. Principle of Operation..........................88 16.11.2. Limitations of Use............................88 16.11.3. Calibrating the CDOM Electrode........................89 © 2017 Aquaread ® Ltd. www.aquaread.com Page 6 of 141...
Page 7 18.5. U ®......................115 PLOADING QUAMETER 18.6. D GPS C ..........................126 ISPLAYING ORDINATES 18.7. O ..............................126 CREEN 18.8. S .............................127 AVING OGGED 18.9. R ............................127 ETRIEVING OGGED 18.10. E ..............................127 XPORTING © 2017 Aquaread ® Ltd. www.aquaread.com Page 7 of 141...
Page 8 RIOR ETURN 19.2. D ..........................132 ECONTAMINATION ERTIFICATE 20. TROUBLESHOOTING.............................133 21. DECLARATION OF CONFORMITY........................134 22. APPENDIX 1. THE TECH BEHIND AQUAREAD’S OPTICAL DO MEASUREMENT SYSTEM....135 22.1. P ............................135 RINCIPLE OF PERATION 22.2. S ..............................136 ENSOR 23. APPENDIX 2. FLOW THROUGH CELL......................137 23.1.
Page 9: Introduction
Aquameter are running earlier firmware, the functionality may differ from that shown in this manual. In this case, contact Aquaread for an earlier version of this manual or return your equipment for firmware upgrade. 2. What’s in the Box? The Aquameter ®...
Page 10: Important Information About The Probe Sleeve & Sleeve End Cap
A perfect example of this is calibrating with the end cap removed then measuring with the end cap fitted (or vice-versa). By changing the physical characteristics of the measurement chamber, you also change the calibration and response of the electrode. © 2017 Aquaread ® Ltd.
Page 11: Top Tips For Successful Measurements Using Optical Electrodes
The extra length also allows the meter to be held in a comfortable position in front of you during normal use. In order to prevent you being dragged into the water in the event of the Probe cable becoming snagged, the lanyard includes a quick-release clip. © 2017 Aquaread ® Ltd.
Page 12: Battery Installation And Care
After a further 15 seconds the backlight will switch off. During normal operation, if you want to activate the backlight without changing the Meter function, simply press the ESC key. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 13: Overview Of The Operating System
When the time and date are correct, press the OK key. Don’t worry if you make a mistake first time round. You can easily get back to these screens later through the MENU key. © 2017 Aquaread ® Ltd.
Page 14: Connecting An Ap-2000
Always ensure the Aquameter ® is switched off prior to connecting or disconnecting an Aquaprobe ® . Align the ◄Aquaread ® logo on the plug body with the red on/off switch on the Aquameter ® , then press the plug into the socket and tighten the retaining collar.
Page 15: What Does It All Mean
If an asterisk (*) character is flashing just below the battery symbol, this indicates that Auto Data Logging is switched on. See Automatic Data Logging in section The table below explains the readings and indicates which to expect with each Meter/Probe combination. © 2017 Aquaread ® Ltd. www.aquaread.com Page 15 of 141...
Page 16: Trend Indication
The electrochemical properties of all solutions change with the solution's temperature. In addition, the response of electrochemical measuring electrodes change with temperature. It is a fundamental, practical requirement in the field of water quality monitoring that test measurements taken at different temperatures can be compared. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 17: Gps Reception
The GPS version of the Aquameter ® (AM-200) contains a built-in GPS/GLONAS receiver and antenna. The antenna is situated at the top of the case, just behind the Aquaread ® Logo. For optimum signal reception, the antenna must be able to ‘see’ a reasonably large amount of the sky.
Page 18 If you switch the meter on indoors, then carry it outside after several minutes, there may be a considerable delay in acquiring satellites. In this case, switch the meter off, then back on again to reset the acquisition process. © 2017 Aquaread ® Ltd.
Page 19: Depth Measurement (Aquaprobes With A - D Suffix Only)
Probe. If two point depth calibration is required, it must be done using an AP-PC KIT and associated AquaCal PC Software. Full depth calibration can not be performed using an Aquameter ® © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 20: Memory Mode
Using the up/down keys, select the electrode you are interested in, then press either the OK key or the right arrow key. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 21: Clearing The Memory
Calibration Setup & Install Select Auto Data Logging by pressing the down arrow key then the right arrow key or the OK key. The Auto Data Logging screen will be displayed. © 2017 Aquaread ® Ltd. www.aquaread.com Page 21 of 141...
Page 22 You can cancel Auto Data Logging at any time by going back into the screen above and setting the Status to OFF. Auto Data Logging will also be cancelled if you switch the Meter off manually. © 2017 Aquaread ® Ltd.
Page 23: Battery And Memory Duration In Low Power Logging Mode
The same rules apply when data is output to a PC running AquaLink Software via the USB cable. The data that is output is always as per the Meter’s current configuration . You can output the data as many times as you like in various Meter configurations. © 2017 Aquaread ® Ltd.
Page 24: Setup & Install
Finally, this screen allows you to set the factor that the Meter uses to calculate Total Dissolved Solids from Specific EC. This is the TDS Fact: (TDS = EC x TDS Fact) and can be set anywhere between 0.00 and 1.00. Default value is 0.65. © 2017 Aquaread ® Ltd.
Page 25: Aux Socket Assignment (Not Applicable To The Ap-700)
This is because the assignment data is held in the Aquaprobe ® , not in the Aquameter ® When the Socket Assignment option has been selected, the following screen will be displayed. © 2017 Aquaread ® Ltd. www.aquaread.com Page 25 of 141...
Page 26 Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. If you subsequently remove an electrode, be sure to set the socket assignment back to EMPTY. © 2017 Aquaread ® Ltd. www.aquaread.com Page 26 of 141...
Page 27: Rapidcal Calibration Method
As a general rule, pH and EC should be calibrated as close to 25ºC as possible. Optical electrodes should be calibrated as close to their deployment temperature as possible. In order to standardise calibration techniques, Aquaread provide plastic calibration bottles ®...
Page 28 When calibration is complete, press OK then ESC to return to normal reading mode. Important If you have ISE electrodes fitted to your Aquaprobe ® , you must now remove the rubber sealing caps from all the ISE electrodes. © 2017 Aquaread ® Ltd. www.aquaread.com Page 28 of 141...
Page 29: To Check / Calibrate The 100% Do Saturation Point In Damp Air
ESC repeatedly to return to normal reading mode. Finally, if you have a Turbidity electrode fitted, you should calibrate the zero point now using fresh, still mineral water. Refer to section 16.4.7 Turbidity Zero Point Calibration . © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 30: Calibration Error Messages
In some cases, if there has been a serious calibration error, the easiest way to rectify the situation is to reset the Probe to its factory defaults. To do this, first bring up the Calibration screen: © 2017 Aquaread ® Ltd.
Page 31: Calibration Reports
In order to recall the calibration data for a certain electrode on the Meter, first select the calibration screen for that electrode. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 32 Pushing the left or right arrow keys will toggle this screen with the calibration report screen. PH7 Offset:+01.2mV PH4 Slope:56.8mV/pH PH10 Slope:56.3mV/pH ORP Offset:-02.6mV The calibration report screen displays the calibration report values from the last calibration. To exit this screen press the ESC key. © 2017 Aquaread ® Ltd. www.aquaread.com Page 32 of 141...
Page 33: After Use
TIP: Occasional application of a smear of silicone grease or similar lubricant to the connector O-rings and thread, Sleeve thread, the protective Sleeve End Cap O-ring and the inside rim of the lower Probe Sleeve will make fitting and removal of these parts easier. © 2017 Aquaread ® Ltd.
Page 34: General Probe Maintenance
Please note: The photograph shows the AP-2000 with Optical DO. The AP-700 and AP- 800 models may have either Optical DO or Galvanic DO sensors. Galvanic DO caps and later model Optical DO caps do not have the red warning label fitted to the DO cap. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 35: Ph/Orp Electrode Calibration And Maintenance
It is very important that the pH/ORP electrode is kept moist when not in use. This is achieved by always fitting the storage cap, which incorporates a sponge that should be soaked in a special storage solution. © 2017 Aquaread ® Ltd.
Page 36: Calibrating Ph
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 37 Retrieval. Press OK then ESC repeatedly to return to normal reading mode. Remove the Probe from the calibration bottle, rinse thoroughly in de-ionised water, shake off any excess and dry the outer sleeve with a soft cloth. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 38: Calibrating The Second Point
13.8. Servicing the pH Electrode Remove the pH or combined pH/ORP electrode from the Probe body (see Electrode Removal and Replacement). Rinse with methyl alcohol. Replace the electrode. Re-calibrate. © 2017 Aquaread ® Ltd. www.aquaread.com Page 38 of 141...
Page 39: Calibrating Orp
Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2017 Aquaread ® Ltd. www.aquaread.com Page 39 of 141...
Page 40 Remove the Probe from the calibration bottle, rinse thoroughly in fresh water, shake off any excess and dry the outer sleeve with a soft cloth. Dampen the sponge in the storage cap with storage solution and fit it to the pH/ORP electrode. ORP calibration is now complete. © 2017 Aquaread ® Ltd.
Page 41: Converting Orp Readings To The Hydrogen Scale
The ORP reference electrode used in Aquaread ® combination electrodes is a 3MPK1 silver...
Page 42: Do Electrode Calibration And Maintenance
Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC TURB © 2017 Aquaread ® Ltd. www.aquaread.com Page 42 of 141...
Page 43: Calibrating The Do 100% Saturation Point In Moist Air
14.4.2. Calibrating the DO 100% Saturation Point in Moist Air 1. Wash the probe thoroughly in fresh water, then shake off ensuring there are no droplets adhering to the DO membrane. © 2017 Aquaread ® Ltd. www.aquaread.com Page 43 of 141...
Page 44: Replacing A Galvanic Do Electrode Membrane Cap
9. Wait at least six hours (preferably over-night) to allow any oxygen dissolved in the filling solution to be consumed. 10. Carry out both Zero point and 100% point DO calibration as described earlier. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 45: Optical Do Electrode
If your Aquaprobe ® is fitted with an Optical DO electrode, the following sections apply. See Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System for more technical details of the Optical DO measurement system. Unlike the Galvanic DO electrode, the Optical DO electrode does not consume oxygen during operation so does not require a constant flow of water or stirring.
Page 46: Calibrating The Do 100% Saturation Point In Moist Air
0.8 and 1.5 (at 25°C). If the value returned is less than 0.8, the Optical DO Cap should be replaced. These values are not stored in memory so should be noted down in a calibration record book for the probe. © 2017 Aquaread ® Ltd.
Page 47: Replacing The Optical Do Cap
5. Carry out both Zero point and 100% point DO calibration as described earlier. Please Note: It is essential when replacing the Optical DO Cap to calibrate the Zero point BEFORE calibrating the 100% point. © 2017 Aquaread ® Ltd.
Page 48: Ec / Temperature Electrode Calibration And Maintenance
® dealers or can be easily manufactured by adding 33.03 grams of laboratory grade sodium chloride (99.9% pure) to 1 litre of deionised water. RapidCal is available from all Aquaread ® dealers. The Probe sleeve forms an integral, working part of the Probe’s EC measurement system, and MUST be fitted during calibration and measurement for correct operation.
Page 49 The screen will change to: PLEASE WAIT Stabilising 000% If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Cell constant:0.98 Calibrating 100% Press [OK] © 2017 Aquaread ® Ltd. www.aquaread.com Page 49 of 141...
Page 50: Verifying Ec Calibration
The Aquaprobe's temperature sensor is built into the oval resin pocket located on the back of the EC sensor. The temperature sensor is extremely linear and by default is set up to read within +/- 0.5°C of the true temperature, which is ample for most applications. © 2017 Aquaread ® Ltd.
Page 51 If the offset has been correctly input, the Aquameter ® will now be reading the corrected temperature. The temperature correction offset is stored in the Aquaprobe ® and applied at all times going forward. © 2017 Aquaread ® Ltd. www.aquaread.com Page 51 of 141...
Page 52: Optional Optical Electrodes Calibration And Maintenance
Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. This is especially important when using the Turbidity electrode, which can display negative readings if the zero point is not correctly calibrated. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 53: Top Tips For Successful Measurements Using Optical Electrodes
If you attempt to calibrate an upper calibration point without first calibrating the ZERO point, a calibration error will occur. 16.3. Fluorescent Electrode Grab Sample Correction Factor A unique feature of the Aquaread ® fluorescent type electrodes is the ability to include a correction factor based upon a grab sample.
Page 54: 2000-Turb Turbidity Electrode
Electrodes. The turbidity electrode, when fitted, sits adjacent to the optical DO electrode. If the optical DO electrode has a red warning label fitted (see photo below), the label must be removed in order to prevent reflections. © 2017 Aquaread ® Ltd.
Page 55: Precautions During Use
The Zero NTU point must always be calibrated first, followed by the other two points, all within the same calibration session (i.e. without turning the Aquameter ® off). © 2017 Aquaread ® Ltd. www.aquaread.com Page 55 of 141...
Page 56: Turbidity Zero Point Calibration
OK or right arrow key to select TURB. The screen will change to: CALIBRATE TURB ZERO? [01/Jan/14]  1000? [01/Jan/14] [01/Jan/14] The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread ® Ltd. www.aquaread.com Page 56 of 141...
Page 57: Verifying The Zero Calibration
Three-quarters fill a calibration bottle with the solution and drop the Probe in all the way. Again, agitate and swirl the Probe and bottle several times in order to remove any air bubbles that may be clinging to the Turbidity electrode. © 2017 Aquaread ® Ltd.
Page 58: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 59: 2000/5000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode
When a BGA-PC electrode is first installed, it MUST be calibrated at both points in order to set the electrode's relative sensitivity and establish its slope. Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 60: Calibration Solution Preparation
100μg/L. This solution can now be used as Pt-2 calibration of the BGA-PC sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 61: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread ® Ltd. www.aquaread.com Page 61 of 141...
Page 62: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2017 Aquaread ® Ltd.
Page 63: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 64: 2000/5000-Bga-Pe Saltwater Blue-Green Algae ( Phycoerythrin ) Electrode
When a BGA-PE electrode is first installed, it MUST be calibrated at both points in order to set the electrode's relative sensitivity and establish its slope. Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 65: Calibration Solution Preparation
8μg/L. This solution can now be used as Pt-2 calibration of the BGA-PE sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 66: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread ® Ltd. www.aquaread.com Page 66 of 141...
Page 67: Calibrating Point 2
GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. To calculate a GS Factor, first take measurements using the fully calibrated electrode. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 68: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 69: 2000/5000-Cphyll Chlorophyll Electrode
When a CPHYLL electrode is first installed, it MUST be calibrated at both points in order to set the electrode's relative sensitivity and establish its slope. Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 70: Calibration Solution Preparation
500μg/L. This solution can now be used as Pt-2 calibration of the CPHYLL sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 71: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread ® Ltd. www.aquaread.com Page 71 of 141...
Page 72: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2017 Aquaread ® Ltd.
Page 73: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 74: 2000/5000-Rhod Rhodamine Wt Electrode
This is exactly the same calibration solution that is recommended for calibration of the BGA-PC electrode. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Rhodamine WT standard is recommended: © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 75: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2017 Aquaread ® Ltd. www.aquaread.com Page 75 of 141...
Page 76 The Calibration Report on the top line displays the voltage output from the electrode in millivolts (mV). This value is not stored in memory so should be noted down in a calibration record book for the probe. © 2017 Aquaread ® Ltd.
Page 77: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 78: 2000/5000-Fscein Fluorescein Wt Electrode
In order to 'calibrate' the FSCEIN electrode, a 100µg/L calibration solution of Fluorescein Dye should be used. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Fluorescein Dye is recommended: © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 79: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2017 Aquaread ® Ltd. www.aquaread.com Page 79 of 141...
Page 80 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2017 Aquaread ® Ltd. www.aquaread.com Page 80 of 141...
Page 81: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 82: 2000/5000-Refoil Refined Oil Electrode
Differences in the fluorescent response between various types of oil. • Differences in the fluorescent response caused by temperature. • Differences in the fluorescent response caused by ambient light. • Interference caused by turbidity. • © 2017 Aquaread ® Ltd. www.aquaread.com Page 82 of 141...
Page 83: Special Precautions When Using The Refoil Electrode
Be sure to handle chemicals with care and to read and comply with all health and safety advice. 16.10.6. Serial Dilution 10ppm Napthalene salt can be prepared either as a one or two step process dependent upon the accuracy of the scales used. © 2017 Aquaread ® Ltd. www.aquaread.com Page 83 of 141...
Page 84: Zero Point Calibration
4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal 5. Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2017 Aquaread ® Ltd. www.aquaread.com Page 84 of 141...
Page 85: Calibrating Point 2
Calibrating Point 2 Remove the Probe from the calibration cup, shake off any excess water then dry the outer sleeve with a soft cloth. Pour 300mL of freshly mixed 1-5, naphthalenedisulfonic acid disodium salt calibration © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 86: Calculating And Applying A Grab Sample Factor
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 87: 2000/5000-Cdom/Fdom Chromophoric (Fluorescent) Dissolved Organic Matter
Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing © 2017 Aquaread ® Ltd.
Page 88: Calibrating The Cdom Electrode
In order to 'calibrate' the CDOM electrode, a 100ppb solution of Quinine Sulphate in sulphuric acid can be used. However, since Quinine Sulphate is extremely expensive and sulphuric acid is dangerous to handle, Aquaread Ltd has formulated an equivalent, non toxic standard for use during CDOM electrode calibration. This is available in 600mL bottles.
Page 89 Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Output:2500mV Calibrating 100% Press [OK] © 2017 Aquaread ® Ltd. www.aquaread.com Page 89 of 141...
Page 90: Calibrating Point 2
Now that this GS Factor has been applied to the electrode, all future measurements will be multiplied by 1.25 prior to being displayed. In this way, the electrode has been corrected for the local conditions and species of organic matter. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 91: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 92: Optional Ise Electrodes Calibration And Maintenance
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2017 Aquaread ® Ltd. www.aquaread.com Page 92 of 141...
Page 93: 2000-Amm Ammonium/Ammonia Electrode
® the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2017 Aquaread ® Ltd. www.aquaread.com Page 93 of 141...
Page 94 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2017 Aquaread ® Ltd. www.aquaread.com Page 94 of 141...
Page 95: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2017 Aquaread ® Ltd.
Page 96: Two-Point Calibration
1 only. 17.4.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread ® Ltd. www.aquaread.com Page 96 of 141...
Page 97: 2000-Nit Nitrate Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2017 Aquaread ® Ltd. www.aquaread.com Page 97 of 141...
Page 98 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2017 Aquaread ® Ltd. www.aquaread.com Page 98 of 141...
Page 99: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2017 Aquaread ® Ltd.
Page 100: Two-Point Calibration
1 only. 17.5.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread ® Ltd. www.aquaread.com Page 100 of 141...
Page 101: 2000-Chl Chloride Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2017 Aquaread ® Ltd. www.aquaread.com Page 101 of 141...
Page 102 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2017 Aquaread ® Ltd. www.aquaread.com Page 102 of 141...
Page 103: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2017 Aquaread ® Ltd.
Page 104: Two-Point Calibration
1 only. 17.6.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread ® Ltd. www.aquaread.com Page 104 of 141...
Page 105: 2000-Cal Calcium Electrode
If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. To calibrate the ISE electrode follow these steps: © 2017 Aquaread ® Ltd. www.aquaread.com Page 105 of 141...
Page 106 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% © 2017 Aquaread ® Ltd. www.aquaread.com Page 106 of 141...
Page 107: Point 2
If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported. If this happens, adjust the temperature and try again. © 2017 Aquaread ® Ltd.
Page 108: Two-Point Calibration
1 only. 17.7.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread ® Ltd. www.aquaread.com Page 108 of 141...
Page 109: 2000-Flu Fluoride Electrode
The second batch of 0.5ppm solution must be at least 10ºC cooler. In order to achieve this, one batch of the 0.5ppm solution should be put into a refrigerator and the other two solutions should be put into a water bath at 25ºC. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 110: Three-Point Calibration
Calibration point 1 (Pt-1) is the warm 0.5ppm point. Calibration point 2 (Pt-2) is the warm 5ppm point. Calibration point 3 (Pt-3) is the cool 0.5ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread ® Ltd.
Page 111: Point 3
The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up as shown above. © 2017 Aquaread ® Ltd.
Page 112: Two-Point Calibration
1 only. 17.8.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread ® Ltd. www.aquaread.com Page 112 of 141...
Page 113: Aqualink Pc Software
Windows ® on a stand-alone PC with a minimum screen resolution of 1024 x 768 and an available USB 2.0 socket. 18.1. Downloading AquaLink PC Software from the Aquaread ® website ™ The AquaLink PC Software is available for download using the following link: ™...
Page 114: Uploading Data From Your Aquameter
18.6. Displaying GPS Co-ordinates On the right of the screen, the position at which the data was logged is displayed in the GPS boxes (when logged using an AM-200 GPS Aquameter ® only). © 2017 Aquaread ® Ltd. www.aquaread.com Page 114 of 141...
Page 115: O Nscreen Help
Once a set of logged data has been uploaded from the Aquameter ® , it can be saved on your PC as a Raw Data file. These files use a proprietary Aquaread ® format and are saved with a .amf (Aquameter ®...
Page 116 , Microsoft ® WordPad is the preferred text editor for viewing AquaLink Text Reports as this handles text file formatting better than Microsoft ® Notepad. A typical report cover page follows. © 2017 Aquaread ® Ltd. www.aquaread.com Page 116 of 141...
Page 117: Typical Text Report Cover Page
Blocks of individual readings, laid out in chronological order, follow this cover page. The readings picked out on the cover page can be cross-referenced to the blocks of individual readings using the Tag numbers. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 118: Exporting Excel Files
Please note: Although you have downloaded the Google™ Earth application and are running it from your PC, you still need to be connected to the Internet in order for the application to access satellite images. A typical Google™ Earth image follows. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 119: Google™ Example
If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo. © 2017 Aquaread ® Ltd.
Page 120: Limited Warranty
Meter and Probe sockets, and damage caused by leaking batteries are not covered. If service is required, contact our Service Department directly by email in the first instance (service@aquaread.com). Report the model number, date of purchase, serial number and problem. You will be given a Returns Authorisation number by our Service Department.
Page 121: Decontamination Certificate
Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________ Decontamination Procedure: _______________________________________________________________________ _______________________________________________________________________________________________ Certified by (print name) : ___________________________________ Title: __________________________________ Date: ______________________________________ Signature: __________________________________ © 2017 Aquaread ® Ltd. www.aquaread.com Page 121 of 141...
Page 122: Troubleshooting
This section details some of the common difficulties you may encounter when using the Aquameter ® , Aquaprobe ® s and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@Aquaread.com). Problem Cause / Remedy The Aquameter ® will not ...
Page 123: Declaration Of Conformity
Appendix 7. Troubleshooting Turbidity. 21. DECLARATION OF CONFORMITY Aquaread ® Ltd declares that the equipment described herein is in compliance with the essential requirements and other relevant provisions of Directives 2004/108/EC and 1999/5/EC. © 2017 Aquaread ® Ltd. www.aquaread.com Page 123 of 141...
Page 124: Appendix 1. The Tech Behind Aquaread's Optical Do Measurement System
Aquaprobe AP-700, AP-800 & AP-2000 Instruction Manual 10401-00870 Rev R ® 22. Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System 22.1. Principle of Operation The Aquaread ® AquaPlus™ Optical DO measurement system works on the principle of Dynamic Luminescence Quenching. A gas-permeable chemical known as a luminophore is excited with short bursts of blue light, which causes molecules in the luminophore to emit red photons.
Page 125: Sensor Cap Life
The combination of low duty cycle and variable excitation brightness can stretch the useful life of a sensor cap as far as several years. © 2017 Aquaread ® Ltd.
Page 126: Appendix 2. Flow Through Cell
(8 US gallons/hour), although the Aquaprobe ® is capable of operating at flow rates as low as 15 litres/hour (4 US gallons/hour). Flow rates above 60 litres/hour (16 US gallons/hour) are not recommended. © 2017 Aquaread ® Ltd. www.aquaread.com Page 126 of 141...
Page 127: Caution
Reduce collar. pressure / flow rate. Probe is forced up out of the ✔ Operating pressure is much too high. Reduce Flowcell during use. pressure / flow rate. © 2017 Aquaread ® Ltd. www.aquaread.com Page 127 of 141...
Page 128: Appendix 3. Fitting Aux Electrodes
Apply a small amount of silicone grease (supplied with the Aquaprobe ® ) to the threaded section and the O-ring of the AUX Electrode (see photograph). ENSURE NO GREASE IS APPLIED TO THE GOLD CONTACTS. © 2017 Aquaread ® Ltd. www.aquaread.com Page 128 of 141...
Page 129: Socket Assignment And Calibration
2000/5000-REFOIL Refined Oil R-OIL 2000/5000-CDOM CDOM/FDOM CDOM AP-5000/7000 type ISE Electrodes (AUX1 only) Electrode Part No. Function Aquameter ® Selection 5000/7000-AMM Ammonium/Ammonia 5000/7000-CHL Chloride 5000/7000-FLU Fluoride 5000/7000-NIT Nitrate 5000/7000-CAL Calcium © 2017 Aquaread ® Ltd. www.aquaread.com Page 129 of 141...
Page 130 Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. If you subsequently remove an electrode, be sure to replace the blanking plug and set the socket assignment back to EMPTY. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 131: Appendix 4. Standard Electrodes Detailed Specification
For this reason, when using the standard pH/ORP electrode, the water under test must have a minimum EC (electrical conductivity) of 100µS/cm. Special low EC pH electrodes are available to special order. © 2017 Aquaread ® Ltd.
Page 132: Appendix 5. Optical Electrodes Detailed Specification And Faqs
Gas or Liquid Chromatography in a laboratory although if grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen in order to improve the accuracy of future readings. © 2017 Aquaread ® Ltd.
Page 133: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why
CDOM/FDOM Repeatability ± 10% of reading 10 µg/L (Quinine Sulphate) Aquaread® Ltd reserves the right to change specifications without notice Notes: 1. MLD (Minimmum Level of Detection) is the minimum value the electrode is physically capable of measuring. 2. MLR (Minimum Level of Repeatability) is the value below which optical electrode readings become generally unreliable and unrepeatable (unless taken under ideal conditions) due to interfering factors such as refraction from visible air bubbles and microscopic aeration.
Page 134: What Is The Accuracy Of The Optical Electrodes
If grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen of each fluorescent type electrode in order to improve the accuracy of future readings. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 135: Appendix 6. Ise Electrodes Detailed Specification
4. All ion selective electrodes exhibit calibration drift over time. Drift should not be a major problem where the electrodes can be frequently calibrated. However, if the electrodes are to be used in long-term deployment studies, drift is almost certain to occur. © 2017 Aquaread ® Ltd.
Page 136: Special Notes Concerning Ise Electrodes During Ph Calibration
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2017 Aquaread ® Ltd. www.aquaread.com Page 136 of 141...
Page 137: Appendix 7. Troubleshooting Turbidity
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread ® Ltd.
Page 138: About Turbidity Measurement
If you experience negative turbidity readings, thoroughly clean the Probe then re-zero in completely clean water. Still, bottled mineral water is recommended for zeroing the electrode as it is cheap and readily available. Never use sparkling or carbonated water. © 2017 Aquaread ® Ltd.
Page 139: Aeration
10mm to the probe sleeve. 28.7. Sensor Saturation Sensor saturation is a very rare problem that can occur in shallow water when combined with very bright sunlight. © 2017 Aquaread ® Ltd. www.aquaread.com Page 139 of 141...
Page 140: Ltd
➢ Always calibrate and zero the electrode as close to your sample temperature as possible. ➢ Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. © 2017 Aquaread ® Ltd. www.aquaread.com...
Page 141: References
10401-00870 Rev R ® 28.9. References This section is based upon Aquaread's experience in the field and information from the following sources. ➢ National Field Manual For the Collection of Water-Quality Data, Turbidity section 6.7, Revised by Chauncey w. Anderson, USGS, 2004.

This manual is also suitable for:

Aquaprobe ap-2000 Aquaprobe ap-700

Aquaread Aquaprobe AP-800 Instruction Manual

What's New in this Manual

1 Introduction

2 What's in the Box

3 Battery Installation and Care

4 Overview of the Operating System

5 Connecting an Ap-2000

6 Taking Measurements

7 Depth Measurement (Aquaprobes with a - D Suffix Only)

8 Memory Mode

9 Setup & Install

10 Rapidcal Calibration Method

11 After Use

12 General Probe Maintenance

13 Ph/Orp Electrode Calibration and Maintenance

14 Do Electrode Calibration and Maintenance

15 Ec / Temperature Electrode Calibration and Maintenance

16 Optional Optical Electrodes Calibration and Maintenance

17 Optional Ise Electrodes Calibration and Maintenance

18 Aqualink Pc Software

19 Limited Warranty

20 Troubleshooting

21 Declaration of Conformity

22 Appendix 1. the Tech Behind Aquaread's Optical Do Measurement System

23 Appendix 2. Flow through Cell

24 Appendix 3. Fitting Aux Electrodes

25 Appendix 4. Standard Electrodes Detailed Specification

26 Appendix 5. Optical Electrodes Detailed Specification and Faqs

27 Appendix 6. Ise Electrodes Detailed Specification

28 Appendix 7. Troubleshooting Turbidity

Quick Links

Troubleshooting

Need help?

Questions and answers

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Summary of Contents for Aquaread Aquaprobe AP-800