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- Page 1 Instruction Manual for the Aquaprobe Lite ® Optical Water Quality Probe and associated Aquameter ® , Utilities & Accessories ® Aquameter Software Version 6.00 and Above AP-Lite Software Version 4.00 and Above Document No. 10401-00976 Revision: F Date: February 19, 2015...
- Page 2 Notwithstanding the foregoing, in no event shall Aquaread ® Ltd and/or its affiliates aggregate liability arising under or in connection with Aquaread ® products, regardless of the number of events, occurrences, or claims giving rise to liability, be in excess of the price paid by the purchaser for the Aquaread product.
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Page 3: Table Of Contents
11.3. 2000-TURB T ...........................24 URBIDITY LECTRODE 11.3.1. About Turbidity..............................24 11.3.2. Precautions During Use..........................25 11.3.3. Negative Turbidity Readings..........................25 11.3.4. Calibrating the Turbidity Electrode.......................26 11.3.5. Calibration Points............................26 11.3.6. Turbidity Zero Point Calibration........................26 © 2015 Aquaread Ltd. www.aquaread.com Page 3 of 72 ®... - Page 4 EFINED LECTRODE 11.9.1. Principle of Operation...........................49 11.9.2. Limitations of Use............................49 11.9.3. Special Precautions When Using the REFOIL Electrode................50 11.9.4. Calibrating the REFOIL Electrode.......................50 11.9.5. Calibration Solution Preparation........................50 11.9.6. Serial Dilution..............................50 © 2015 Aquaread Ltd. www.aquaread.com Page 4 of 72 ®...
- Page 5 CAN SEE ALGAE IN THE WATER BUT MY SENSOR IS GIVING LOW READINGS 18.4. W ? ................72 HAT IS THE ANGE AND ESOLUTION OF THE PTICAL LECTRODES 18.5. W ?....................73 HAT IS THE CCURACY OF THE PTICAL LECTRODES © 2015 Aquaread Ltd. www.aquaread.com Page 5 of 72 ®...
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Page 6: Introduction
Aquameter ® are running earlier software, the functionality may differ from that shown in this manual. In this case, contact Aquaread for an earlier version of this manual or return your equipment for software upgrade. 2. What’s in the Box? The AP-Lite is supplied with the following: ... -
Page 7: About The Probe Sleeve And End Cap
A perfect example of this is calibrating with the end cap removed then measuring with the end cap fitted (or vice-versa). By changing the physical characteristics of the measurement chamber, you also change the calibration and response of the electrode. © 2015 Aquaread Ltd. www.aquaread.com Page 7 of 72 ®... -
Page 8: Top Tips For Successful Measurements Using Optical Electrodes
In order to obtain accurate results, data obtained with a fluorescent electrode in the field must be post-calibrated with data from standard laboratory analysis of grab samples acquired during the study. © 2015 Aquaread Ltd. www.aquaread.com Page 8 of 72... -
Page 9: Battery Installation And Care
After a further 15 seconds the backlight will switch off. During normal operation, if you want to activate the backlight without changing the Meter function, simply press the ESC or OK key. © 2015 Aquaread Ltd. www.aquaread.com Page 9 of 72 ®... -
Page 10: Overview Of The Operating System
When the time and date are correct, press the OK key. Don’t worry if you make a mistake first time round. You can easily get back to these screens later through the MENU key. © 2015 Aquaread Ltd. www.aquaread.com Page 10 of 72 ®... -
Page 11: Fitting Electrodes
Chlorophyll Cphl 2000-BGA-PC Phycocyanin (Blue-Green Algae PC) BGA-PC 2000-BGA-PE Phycoerythrin (Blue-Green Algae PE) BGA-PE 2000-RHOD Rhodamine WT Dye Rhod 2000-FSCEIN Fluorescein Dye Fcein 2000-REFOIL Refined Oil R-OIL 2000-CDOM CDOM/FDOM CDOM © 2015 Aquaread Ltd. www.aquaread.com Page 11 of 72 ®... -
Page 12: Initial Calibration
YOUR NEW ELECTRODE WILL NOT GIVE SENSIBLE READINGS UNTIL IT HAS BEEN FULLY CALIBRATED. Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. © 2015 Aquaread Ltd. www.aquaread.com Page 12 of 72 ®... -
Page 13: Connecting The Ap-Lite To An Aquameter
Probe to allow easy tightening of the collar. To connect the Extension Cable to the AP-Lite, align the white dot on the AP-Lite with the ◄AQUAREAD logo on the plug body, then press the plug into the socket and tighten the retaining collar fully. -
Page 14: Taking Measurements
During calibration of the optical electrodes, variations in the calibration solutions due to temperature are automatically compensated for. During the measurement, temperature is automatically compensated for. © 2015 Aquaread Ltd. www.aquaread.com Page 14 of 72... -
Page 15: Gps Reception
(AM-200) contains a built-in GPS receiver and antenna. The antenna is situated at the top of the case, just behind the AQUAREAD Logo. For optimum signal reception, the antenna must be able to ‘see’ a reasonably large amount of the sky. The GPS receiver will not work indoors or when shielded from the sky by any solid structure. -
Page 16: Memory Mode
Point 2. If the date field is dashed (==/===/==), this means the electrode had not been calibrated at that point. To exit this screen press the ESC key or the left arrow key. © 2015 Aquaread Ltd. www.aquaread.com Page 16 of 72... -
Page 17: Clearing The Memory
If permanent display logging mode was selected (Status set to ON), the Meter will record a full set of data automatically at the set rate until either the memory is full or the batteries go flat. © 2015 Aquaread Ltd. www.aquaread.com Page 17 of 72 ®... -
Page 18: Battery And Memory Duration In Low Power Logging Mode
(at 21°C or greater). Useful Tip: If you want GPS data logged in association with your other data, ensure the Meter is positioned face up with a clear view of the sky. © 2015 Aquaread Ltd. www.aquaread.com Page 18 of 72... -
Page 19: Setup & Install
The first line allows you to choose between displaying Barometric pressure in millibars (mb) or in mm of mercury (mmHg). The second line allows you to choose between displaying altitude in metres (M) or feet (F). © 2015 Aquaread Ltd. www.aquaread.com Page 19 of 72 ®... -
Page 20: About Calibration
You are trying to calibrate an upper CAL ZERO then without switching the calibration point on an optical electrode FIRST Aquameter off, calibrate the without first calibrating the zero point. upper point. © 2015 Aquaread Ltd. www.aquaread.com Page 20 of 72 ®... -
Page 21: Resetting To Factory Calibration Defaults
Please note however, in order to maximise the value of this feature, all calibrations must be performed at the same temperature otherwise the recorded values will not be comparable over time. © 2015 Aquaread Ltd. www.aquaread.com Page 21 of 72... -
Page 22: Electrode Calibration And Maintenance
The photograph to the right was taken in a calibration tube after fresh water was poured in. The bubbles are clearly visible in the light beam. © 2015 Aquaread Ltd. www.aquaread.com Page 22 of 72... -
Page 23: Top Tips For Successful Measurements Using Optical Electrodes
Particle size relative to the wavelength of the transmitted light, particle shape and refractive index modify the distribution of scattered light. Sample colour, (particularly dark colours) can also reduce a certain portion of the scattered light by varying degrees. © 2015 Aquaread Ltd. www.aquaread.com Page 23 of 72 ®... -
Page 24: Precautions During Use
Probe and re-calibrate. Do not leave the Probe sitting in aerated water, the bubbles will simply cling to the inside surface of the Probe and make the problem worse. © 2015 Aquaread Ltd. www.aquaread.com Page 24 of 72... -
Page 25: Calibrating The Turbidity Electrode
Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. © 2015 Aquaread Ltd. www.aquaread.com Page 25 of 72 ®... -
Page 26: Verifying The Zero Calibration
Calibration Report, which will show the voltage output from the Turbidity Receiver Electrode in millivolts (mV). Press the OK key to continue. Rinse the probe thoroughly then repeat this procedure for the third point. © 2015 Aquaread Ltd. www.aquaread.com Page 26 of 72... -
Page 27: Errors During Calibration
Turbidity Science, Michael J. Sadar, Hach Company 1998. Guidelines and Standard Procedures for continuous Water-Quality Monitors: Site Selection, Field Operation, Calibration, Record Computation and Reporting, Richard J. Wagner et al., USGS Reston VA Meeting, 2000. © 2015 Aquaread Ltd. www.aquaread.com Page 27 of 72... -
Page 28: 2000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode
Subsequently, a single point (Zero) calibration should be carried out at the beginning of each day's use, as close to the measurement temperature as possible. Full two-point calibration should be carried out every few months. © 2015 Aquaread Ltd. www.aquaread.com Page 28 of 72 ®... -
Page 29: Calibration Solution Preparation
The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. 11.4.6. Zero Point Calibration To calibrate the zero point, follow these steps: © 2015 Aquaread Ltd. www.aquaread.com Page 29 of 72 ®... -
Page 30: Calibrating Point 2
Fill a calibration bottle with freshly mixed Rhodamine calibration solution and drop the Probe in all the way. Again, agitate the Probe several times in order to remove any air bubbles that may be clinging to the electrode. © 2015 Aquaread Ltd. www.aquaread.com Page 30 of 72 ®... -
Page 31: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 31 of 72 ®... -
Page 32: 2000-Bga-Pe Saltwater Blue-Green Algae ( Phycoerythrin ) Electrode
Subsequently, a single point (Zero) calibration should be carried out at the beginning of each day's use, as close to the measurement temperature as possible. Full two-point calibration should be carried out every few months. © 2015 Aquaread Ltd. www.aquaread.com Page 32 of 72 ®... -
Page 33: Calibration Solution Preparation
To calibrate the zero point, follow these steps: 1. Fill a calibration bottle with mineral water, wash the Probe in mineral water, then drop the Probe in all the way. The Sleeve End Cap must be fitted. © 2015 Aquaread Ltd. www.aquaread.com Page 33 of 72 ®... -
Page 34: Calibrating Point 2
Follow the procedure detailed above for Zero point calibration as far as step 4, then select Pt-2. Wait while the Meter stabilises and calibrates. © 2015 Aquaread Ltd. www.aquaread.com Page 34 of 72... -
Page 35: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 35 of 72 ®... -
Page 36: 2000-Cphyll Chlorophyll Electrode
Subsequently, a single point (Zero) calibration should be carried out at the beginning of each day's use, as close to the measurement temperature as possible. Full two-point calibration should be carried out every few months. © 2015 Aquaread Ltd. www.aquaread.com Page 36 of 72 ®... -
Page 37: Calibration Solution Preparation
Probe in all the way. The Sleeve End Cap must be fitted. Gently agitate the Probe several times in order to remove any air bubbles that may be clinging to the electrode. © 2015 Aquaread Ltd. www.aquaread.com Page 37 of 72... -
Page 38: Calibrating Point 2
Pt-2. Wait while the Meter stabilises and calibrates. After successful calibration, the ‘Calibrating 100%’ screen will be displayed along with the Calibration Report, which will show the voltage output from the electrode in millivolts (mV). © 2015 Aquaread Ltd. www.aquaread.com Page 38 of 72 ®... -
Page 39: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 39 of 72 ®... -
Page 40: 2000-Rhod Rhodamine Wt Electrode
This is exactly the same calibration solution that is recommended for calibration of the BGA-PC electrode. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Rhodamine WT standard is recommended: © 2015 Aquaread Ltd. www.aquaread.com Page 40 of 72... -
Page 41: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. CALIBRATE Rhod ZERO? [01/Jan/12] Pt-2? [01/Jan/12] © 2015 Aquaread Ltd. www.aquaread.com Page 41 of 72 ®... -
Page 42: Calibrating Point 2
Calibration is now complete. 11.7.8. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2015 Aquaread Ltd. www.aquaread.com Page 42 of 72 ®... -
Page 43: Lens And Sleeve Maintenance
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 43 of 72 ®... -
Page 44: 2000-Fscein Fluorescein Wt Electrode
In order to 'calibrate' the FSCEIN electrode, a 100µg/L calibration solution of Fluorescein Dye should be used. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Fluorescein Dye is recommended: © 2015 Aquaread Ltd. www.aquaread.com Page 44 of 72... -
Page 45: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. CALIBRATE Fcein ZERO? [01/Jan/12] Pt-2? [01/Jan/12] © 2015 Aquaread Ltd. www.aquaread.com Page 45 of 72 ®... -
Page 46: Calibrating Point 2
Calibration is now complete. 11.8.8. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2015 Aquaread Ltd. www.aquaread.com Page 46 of 72 ®... -
Page 47: Lens And Sleeve Maintenance
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 47 of 72 ®... -
Page 48: 2000-Refoil Refined Oil Electrode
Differences in the fluorescent response between various types of oil. • Differences in the fluorescent response caused by temperature. • Differences in the fluorescent response caused by ambient light. • Interference caused by turbidity. • © 2015 Aquaread Ltd. www.aquaread.com Page 48 of 72 ®... -
Page 49: Special Precautions When Using The Refoil Electrode
Weigh out 10.5mg of the recommended salt and add to 1L of deionized water in a volumetric flask. Invert or mix until all salt has dissolved. This gives the Pt-2 10ppm stock solution required for calibration. © 2015 Aquaread Ltd. www.aquaread.com Page 49 of 72 ®... -
Page 50: Zero Point Calibration
4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/ORP DO/EC Aux Electrodes Select Aux Electrodes. The screen will change to: © 2015 Aquaread Ltd. www.aquaread.com Page 50 of 72 ®... -
Page 51: Calibrating Point 2
Pour 300mL of freshly mixed 1-5, naphthalenedisulfonic acid disodium salt calibration solution into a clean calibration cup then gently lower the Probe in all the way. © 2015 Aquaread Ltd. www.aquaread.com Page 51 of 72... -
Page 52: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 52 of 72 ®... -
Page 53: 2000-Cdom/Fdom C
Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing concentrations. © 2015 Aquaread Ltd. www.aquaread.com Page 53 of 72... -
Page 54: Calibrating The Cdom Electrode
In order to 'calibrate' the CDOM electrode, a 100ppb solution of Quinine Sulphate in sulphuric acid can be used. However, since Quinine Sulphate is extremely expensive and sulphuric acid is dangerous to handle, Aquaread Ltd has formulated an equivalent, non toxic standard for use during CDOM electrode calibration. This is available in 600mL bottles. - Page 55 The Calibration Report on the top line displays the voltage output from the electrode in millivolts (mV). This value is not stored in memory so should be noted down in a calibration record book for the probe. © 2015 Aquaread Ltd. www.aquaread.com Page 55 of 72 ®...
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Page 56: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2015 Aquaread Ltd. www.aquaread.com Page 56 of 72 ®... -
Page 57: After Use
Decon 90. These products can strip the anodised finish from the Probe and damage the plastic and rubber components. Damage caused by the use of aggressive cleaning agents or solvents is not covered by your warranty. © 2015 Aquaread Ltd. www.aquaread.com Page 57 of 72 ®... -
Page 58: Aqualink Pc Software
PC with a minimum screen resolution of 1024 x 768, a CD drive and an available USB 2.0 socket. ® 13.1. Downloading AquaLink PC Software from the Aquaread website ™ The AquaLink PC Software is available for download using the following link: ™... -
Page 59: Uploading Data From Your Aquameter
3D Position fix. GPS position is also displayed as an Ordnance Survey Great Britain (OSGB) grid reference, (if the position falls within the United Kingdom) and UTM (Universal Transverse Mercator) co-ordinates. © 2015 Aquaread Ltd. www.aquaread.com Page 59 of 72... -
Page 60: O Nscreen Help
® , it can be saved on ® your PC as a Raw Data file. These files use a proprietary Aquaread format and are saved with a .amf (aquameter file) extension. To save the uploaded data, click the ‘Save as Raw Data’ button. You will be asked for a file name in the normal Windows ®... -
Page 61: Exporting Excel Files
Please note: Although you have downloaded the Google™ Earth application and are running it from your PC, you still need to be connected to the Internet in order for the application to access satellite images. A typical Google™ Earth image follows. © 2015 Aquaread Ltd. www.aquaread.com Page 61 of 72... -
Page 62: Google™ Example
If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo. © 2015 Aquaread Ltd. www.aquaread.com Page 62 of 72 ®... -
Page 63: Limited Warranty
Meter and Probe sockets, and damage caused by leaking batteries are not covered. If service is required, contact our Service Department directly by email in the first instance (service@aquaread.com). Report the model number, date of purchase, serial number and problem. You will be given a Returns Authorisation number by our Service Department. -
Page 64: Decontamination Certificate
Postal code: ___________________________________ Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________ Decontamination Procedure:______________________________________________________________________ ______________________________________________________________________________________________ Certified by (print name) : ___________________________________ Title:__________________________________ Date: ______________________________________ Signature: __________________________________ © 2015 Aquaread Ltd. www.aquaread.com Page 64 of 72 ®... -
Page 65: Troubleshooting
This section details some of the common difficulties you may encounter when using the Aquameter ® , AP-Lite and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@aquaread.com). Problem Cause / Remedy The Aquameter ®... -
Page 66: Declaration Of Conformity
® 16. DECLARATION OF CONFORMITY Aquaread ® Ltd declares that the equipment described herein is in compliance with the essential requirements and other relevant provisions of Directives 2004/108/EC and 1999/5/EC. © 2015 Aquaread Ltd. www.aquaread.com Page 66 of 72 ®... -
Page 67: Appendix 1. Flow Through Cell
4. Carry out a full calibration with the Adaptor fitted. Re-calibration at this point is extremely important because by fitting the Adaptor Collar, you have altered the dimensions of the measurement chamber and hence the calibration. © 2015 Aquaread Ltd. www.aquaread.com Page 67 of 72 ®... -
Page 68: Spigot Installation
Never clean the Flowcell with concentrated acid or alkaline based cleaning products such as Decon 90. These products can strip the anodised finish from the Flowcell and damage the plastic components. © 2015 Aquaread Ltd. www.aquaread.com Page 68 of 72... -
Page 69: Flowcell Troubleshooting
Operating pressure is too high. Reduce pressure / flow rate. screw collar. Probe is forced up out of Operating pressure is much too high. Reduce pressure / the Flowcell during use. flow rate. © 2015 Aquaread Ltd. www.aquaread.com Page 69 of 72 ®... -
Page 70: Appendix 2. Standard Electrodes Detailed Specification And Faqs
The electrode is not intended for absolute, quantitative measurements. This can only really be done using Gas or Liquid Chromatography in a laboratory. © 2015 Aquaread Ltd. www.aquaread.com Page 70 of 72... -
Page 71: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why
CDOM/FDOM Repeatability ± 10% of reading 10 µg/L (Quinine Sulphate) Aquaread® Ltd reserves the right to change specifications without notice Notes: MLD (Minimmum Level of Detection) is the minimum value the electrode is physically capable of measuring. MLR (Minimum Level of Repeatability) is the value below which optical electrode readings become generally unreliable and unrepeatable (unless taken under ideal conditions) due to interfering factors such as refraction from visible air bubbles and microscopic aeration. -
Page 72: What Is The Accuracy Of The Optical Electrodes
In order to obtain accurate results, data obtained with a fluorescent electrode in the field must be post-calibrated with data from standard laboratory analysis of grab samples acquired during the study. © 2015 Aquaread Ltd. www.aquaread.com Page 72 of 72...
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