Page 1 Instruction Manual for the Aquaprobe ® AP-7000 Multiparameter Water Quality Probe and associated Aquameter ® , Utilities & Accessories Aquameter ® firmware Version 6.20 and Above AP-7000 firmware Version 4.07 and Above Document No. 10401-00894 Revision: M Date: March 27, 2017...
Page 2 Notwithstanding the foregoing, in no event shall Aquaread ® Ltd and/or its affiliates aggregate liability arising under or in connection with Aquaread ® products, regardless of the number of events, occurrences, or claims giving rise to liability, be in excess of the price paid by the purchaser for the Aquaread ®...
Page 3: What's New In This Manual
Aquameters currently running firmware revisions lower than 4.50 can not be upgraded due to lack of sufficient memory space. Other Manual Additions This manual also includes two new sections. These are: ➢ Appendix 7. Troubleshooting Turbidity ➢ Appendix 8. Flow Through Cell © 2017 Aquaread Ltd. www.aquaread.com Page 3 of 143 ®...
Page 4: Table Of Contents
ESSAGES 10.5. R ......................32 ESETTING TO ACTORY ALIBRATION EFAULTS 10.6. C ..............................32 ALIBRATION EPORTS 10.7. C .......................32 ALIBRATION TORAGE AND ETRIEVAL 11. AFTER USE..................................34 12. THE AP-7000 AUTOMATIC CLEANING SYSTEM.....................35 © 2017 Aquaread Ltd. www.aquaread.com Page 4 of 143 ®...
Page 5 15.5.8. Calculating and Applying a Grab Sample Factor..................64 15.5.9. Errors During Calibration..........................65 15.5.10. Lens and Sleeve Maintenance........................65 15.6. 7000-BGA-PE S .............66 ALTWATER REEN LGAE PHYCOERYTHRIN LECTRODE 15.6.1. Principle of Operation...........................66 15.6.2. Limitations of Use............................66 © 2017 Aquaread Ltd. www.aquaread.com Page 5 of 143 ®...
Page 6 15.11.2. Limitations of Use............................90 15.11.3. Calibrating the CDOM Electrode........................91 15.11.4. Calibration Solutions...........................91 15.11.5. Zero Point Calibration..........................91 15.11.6. Calibrating Point 2............................93 15.11.7. Calculating and Applying a Grab Sample Factor..................93 15.11.8. Errors During Calibration...........................94 © 2017 Aquaread Ltd. www.aquaread.com Page 6 of 143 ®...
Page 7 17.10. E ..............................118 XPORTING 17.11. E ............................118 XPORTING EPORTS 17.12. T ........................120 YPICAL EPORT OVER 17.13. E ® F ............................121 XPORTING XCEL ILES 17.14. E ™ F ...........................121 XPORTING OOGLE ILES © 2017 Aquaread Ltd. www.aquaread.com Page 7 of 143 ®...
Page 8 RIOR ETURN 18.2. D ..........................124 ECONTAMINATION ERTIFICATE 19. TROUBLESHOOTING.............................125 20. DECLARATION OF CONFORMITY........................126 21. APPENDIX 1. THE TECH BEHIND AQUAREAD’S OPTICAL DO MEASUREMENT SYSTEM....127 21.1. P ............................127 RINCIPLE OF PERATION 21.2. S ..............................128 ENSOR 22. APPENDIX 2. FITTING AUX ELECTRODES.....................129 22.1.
Page 9: Introduction
Aquameter ® are running earlier firmware, the functionality may differ from that shown in this manual. In this case, contact Aquaread for an earlier version of this manual or return your equipment for firmware upgrade. 2. What’s in the Box? The Aquameter ®...
Page 10 10401-00894 Rev M ® All Aquaread® Optical Electrodes are incredibly sensitive. For example, the Turbidity electrode is capable of measuring between 0 and 3000NTU with an internal resolution of greater than 0.1NTU. This means that the electrode is able to detect changes in turbidity that are less than 0.003% of the full range! The other optical electrodes have a similar level...
Page 11: Top Tips For Successful Measurements Using Optical Electrodes
Also remember, in order to achieve accurate readings with ISE electrodes, the Probe needs to be placed in flowing water with a minimum flow rate of 0.3m/s over the electrode. © 2017 Aquaread Ltd. www.aquaread.com Page 11 of 143 ®...
Page 12 During long term deployment, the AP-7000 can be suspended by the Extension Cable, but in order to provide a fail-safe for the plug and socket, always Ty-wrap the Extension Cable to the bracket just above the plug. © 2017 Aquaread Ltd. www.aquaread.com Page 12 of 143 ®...
Page 13: Battery Installation And Care
After a further 15 seconds the backlight will switch off. During normal operation, if you want to activate the backlight without changing the Meter function, simply press the ESC key. © 2017 Aquaread Ltd. www.aquaread.com Page 13 of 143...
Page 14: Overview Of The Operating System
When the time and date are correct, press the OK key. Don’t worry if you make a mistake first time round. You can easily get back to these screens later through the MENU key. © 2017 Aquaread Ltd. www.aquaread.com Page 14 of 143 ®...
Page 15: Connecting An Ap-7000
To connect the Extension Cable to the AP-7000, align the coloured dot on the AP-7000 with the ◄AQUAREAD logo on the plug body, then press the plug into the socket and tighten the retaining collar fully. Once the AP-7000 has been connected to the Extension Cable, the Aquameter ®...
Page 16: Taking Measurements
EC:6541µS/cm   TDS:3271mg/L GPS:3D Pos   SAL:03.57 PSU    SSG:01.3σt  GPS:3D Pos    BARO:1013mb DEPTH:01.75m Hit [OK] to zero GPS:3D Pos   © 2017 Aquaread Ltd. www.aquaread.com Page 16 of 143 ®...
Page 17: What Does It All Mean
In addition to the individual trend indications, there is a global stability indication, which is displayed when all readings are stable. This takes the form of a flashing double headed arrow which is displayed at the start of the third line of the display. © 2017 Aquaread Ltd. www.aquaread.com Page 17 of 143 ®...
Page 18: Temperature Compensation
(usually 20ºC or 25ºC) although a variation of up to +/-10ºC makes very little difference in reality. During pH measurement, temperature variation is automatically compensated for. © 2017 Aquaread Ltd. www.aquaread.com Page 18 of 143 ®...
Page 19: Gps Reception
® (AM-200) contains a built-in GPS/GLONAS receiver and antenna. The antenna is situated at the top of the case, just behind the AQUAREAD Logo. For optimum signal reception, the antenna must be able to ‘see’ a reasonably large amount of the sky. The GPS receiver will not work indoors or when shielded from the sky by any solid structure.
Page 20: Depth Measurement
The depth sensor is automatically re-zeroed each time the DO 100% point is calibrated in free air. The depth sensor is factory calibrated at two temperatures and two pressures. No further calibration is required for the lifetime of the sensor. © 2017 Aquaread Ltd. www.aquaread.com Page 20 of 143 ®...
Page 21: Memory Mode
Using the up/down keys, select the electrode you are interested in, then press either the OK key or the right arrow key. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. © 2017 Aquaread Ltd. www.aquaread.com Page 21 of 143...
Page 22: Clearing The Memory
Calibration Setup & Install Select Auto Data Logging by pressing the down arrow key then the right arrow key or the OK key. The Auto Data Logging screen will be displayed. © 2017 Aquaread Ltd. www.aquaread.com Page 22 of 143...
Page 23: Battery And Memory Duration In Low Power Logging Mode
45 mins 30 Days 312 Days 30 mins 26 Days 208 Days 15 mins 18 Days 104 Days 5 mins 8 Days 34 Days 1 min 45 Hours 6.9 Days © 2017 Aquaread Ltd. www.aquaread.com Page 23 of 143 ®...
Page 24: Important Information About Memory Mode
The same rules apply when data is output to a PC running AquaLink Software via the USB cable. The data that is output is always as per the Meter’s current configuration . You can output the data as many times as you like in various Meter configurations. © 2017 Aquaread Ltd. www.aquaread.com Page 24 of 143 ®...
Page 25: Setup & Install
Finally, this screen allows you to set the factor that the Meter uses to calculate Total Dissolved Solids from Specific EC. This is the TDS Fact: (TDS = EC x TDS Fact) and can be set anywhere between 0.00 and 1.00. Default value is 0.65. © 2017 Aquaread Ltd. www.aquaread.com Page 25 of 143 ®...
Page 26: Aux Socket Assignment
The Socket Assignment option is only available if the Aquameter ® is connected to an AP- 7000. This is because the assignment data is held in the Aquaprobe ® , not in the Aquameter ® © 2017 Aquaread Ltd. www.aquaread.com Page 26 of 143 ®...
Page 27 Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. If you subsequently remove an electrode, be sure to set the socket assignment back to EMPTY. © 2017 Aquaread Ltd. www.aquaread.com Page 27 of 143...
Page 28: Rapidcal Calibration Method
The caps should be fitted to all ISE Electrodes during when using RapidCal order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2017 Aquaread Ltd. www.aquaread.com Page 28 of 143 ®...
Page 29: Using Rapidcal
7. Select RapidCal. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until all readings are stable, then it will send the RapidCal command to the Probe, where the calibration takes place. © 2017 Aquaread Ltd. www.aquaread.com Page 29 of 143 ®...
Page 30: To Check / Calibrate The 100% Saturation Point In Damp Air
Finally, if you have any optical electrodes fitted, you should calibrate the zero points now using fresh, still mineral water. Refer to the relevant sections in this manual for advice on zero point calibration of your optical electrodes. © 2017 Aquaread Ltd. www.aquaread.com Page 30 of 143 ®...
Page 31: Calibration Error Messages
If you try to calibrate any of the optical electrodes or the EC without the sleeve, end cap and wiper fitted, you may get an error message. © 2017 Aquaread Ltd. www.aquaread.com Page 31 of 143...
Page 32: Resetting To Factory Calibration Defaults
Meter, this data is transferred for display and logging. This is a major advantage and allows you to use a variety of different Probes with a single Meter, without the need for re-calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 32 of 143...
Page 33 Pushing the left or right arrow keys will toggle this screen with the calibration report screen. PH7 Offset:+01.2mV PH4 Slope:56.8mV/pH PH10 Slope:56.3mV/pH ORP Offset:-02.6mV The calibration report screen displays the calibration report values from the last calibration. To exit this screen press the ESC key. © 2017 Aquaread Ltd. www.aquaread.com Page 33 of 143 ®...
Page 34: After Use
TIP: Occasional application of a smear of silicone grease (provided) to the connector O- rings and thread, Sleeve thread, the protective Sleeve End Cap thread will make fitting and removal of these parts easier. © 2017 Aquaread Ltd. www.aquaread.com Page 34 of 143...
Page 35: The Ap-7000 Automatic Cleaning System
Occasional cleaning or replacement of the wiper brushes may be required due to fouling. This is a simple job and requires no tools. First remove the sleeve then pull the stainless-steel spring pin out of the central shaft. © 2017 Aquaread Ltd. www.aquaread.com Page 35 of 143 ®...
Page 36 ® Next, slide the wiper off the central shaft. Next, slide the top brush carrier out of the wiper. Next, slide the top brush out of the top brush carrier. © 2017 Aquaread Ltd. www.aquaread.com Page 36 of 143 ®...
Page 37 Ensure that the torque arm (see below) is aligned with the corresponding receiving • hole in the end of the wiper. Finally, ensure the spring clip is correctly and securely fitted. © 2017 Aquaread Ltd. www.aquaread.com Page 37 of 143...
Page 38: Important Notes Regarding Long -Term Deployment
It is strongly recommended that after initial deployment, maintenance visits are made on a weekly basis in order to assess the rate of fouling. After several weeks, it should be possible to establish the optimum time interval for routine maintenance at any given site. © 2017 Aquaread Ltd. www.aquaread.com Page 38 of 143 ®...
Page 39: Ph/Orp Electrode Calibration And Maintenance
Electrode Storage Solution each time it is removed and replaced. If a pH/ORP electrode is inadvertently allowed to dry out, it must be re-hydrated by soaking in storage solution for at least one hour prior to use. © 2017 Aquaread Ltd. www.aquaread.com Page 39 of 143 ®...
Page 40: Calibrating Ph
3. Wait until the temperature and pH measurements are completely stable. 4. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF – 104ºF). 5. Press the MENU key then select Calibration. The following screen will be displayed. © 2017 Aquaread Ltd. www.aquaread.com Page 40 of 143 ®...
Page 41 This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2017 Aquaread Ltd. www.aquaread.com Page 41 of 143...
Page 42: Calibrating The Second Point
85%, ‘ERROR 01’ will be flashed on the bottom line of the display. If this occurs, or if the pH reading response becomes slow, recondition the electrode as described below. © 2017 Aquaread Ltd. www.aquaread.com Page 42 of 143...
Page 43: Servicing The Ph Electrode
Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2017 Aquaread Ltd. www.aquaread.com Page 43 of 143 ®...
Page 44 Remove the Probe from the calibration cup, rinse thoroughly in fresh water, shake off any excess and dry the outer sleeve with a soft cloth. Dampen the sponge in the storage cap with storage solution and fit it to the pH/ORP electrode. ORP calibration is now complete. © 2017 Aquaread Ltd. www.aquaread.com Page 44 of 143 ®...
Page 45: Converting Orp Readings To The Hydrogen Scale
The ORP reference electrode used in Aquaread ® combination electrodes is a 3MPK1 silver...
Page 46: Do/Ec Electrode Calibration And Maintenance
The AP-7000 features an optical DO sensor. This sensor does not use a liquid electrolyte and has a black rubber gas-permeable membrane. See Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System for further details. 14.3. Precautions During Use EC measurement is not possible with the Probe central wiper removed as the central wiper forms an integral part of the measurement system.
Page 47 This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2017 Aquaread Ltd. www.aquaread.com Page 47 of 143...
Page 48: Calibrating The Do 100% Saturation Point In Moist Air
2. Unscrew the Optical DO Cap from the end of the DO/EC electrode by rotating it anti- clockwise. Do not touch the exposed optical components. 3. Apply a light smear of silicone grease to the thread and O ring. © 2017 Aquaread Ltd. www.aquaread.com Page 48 of 143...
Page 49 5. Carry out both Zero point and 100% point DO calibration as described earlier. Please Note: It is essential when replacing the Optical DO Cap to calibrate the Zero point BEFORE calibrating the 100% point. © 2017 Aquaread Ltd. www.aquaread.com Page 49 of 143 ®...
Page 50: Calibrating Ec
® dealers or can be easily manufactured by adding 33.03 grams of laboratory grade sodium chloride (99.9% pure) to 1 litre of deionised water. RapidCal is available from all Aquaread ® dealers. The Probe's central wiper forms an integral, working part of the Probe’s EC measurement system, and MUST be fitted during calibration and measurement for correct operation.
Page 51 The screen will change to: PLEASE WAIT Stabilising 000% If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Cell constant:0.98 Calibrating 100% Press [OK] © 2017 Aquaread Ltd. www.aquaread.com Page 51 of 143 ®...
Page 52: Verifying Ec Calibration
DO/EC electrode with a soft cloth or toothbrush and non-abrasive detergent. Never use solvent or alcohol based products to clean the DO/EC electrode. After cleaning, replace the Probe sleeve and re-calibrate. © 2017 Aquaread Ltd. www.aquaread.com Page 52 of 143...
Page 53: Calibrating Temperature
If the offset has been correctly input, the Aquameter ® will now be reading the corrected temperature. The temperature correction offset is stored in the Aquaprobe ® and applied at all times going forward. © 2017 Aquaread Ltd. www.aquaread.com Page 53 of 143 ®...
Page 54: Optional Optical Electrodes Calibration And Maintenance
Another particular problem when trying to measure very low concentrations is air in the form of both visible and microscopic bubbles. These act like tiny prisms and can refract and reflect both the excitation light and the return signal being measured. © 2017 Aquaread Ltd. www.aquaread.com Page 54 of 143 ®...
Page 55: Top Tips For Successful Measurements Using Optical Electrodes
If you attempt to calibrate an upper calibration point without first calibrating the ZERO point, a calibration error will occur. 15.3. Fluorescent Electrode Grab Sample Correction Factor A unique feature of the Aquaread ® fluorescent type electrodes is the ability to include a correction factor based upon a grab sample.
Page 56: 7000-Turb Turbidity Electrode
Sleeve End Cap fitted. If you experience any problems using the Turbidity Electrode, refer to Appendix 7. Troubleshooting Turbidity © 2017 Aquaread Ltd. www.aquaread.com Page 56 of 143 ®...
Page 57: Negative Turbidity Readings
A three point calibration should be carried out once a month to ensure optimum accuracy. Zeroing of the Turbidity electrode is normally carried out automatically during RapidCal (see RapidCal Calibration Method). © 2017 Aquaread Ltd. www.aquaread.com Page 57 of 143 ®...
Page 58: Turbidity Zero Point Calibration
1000? [01/Jan/14] [01/Jan/14] The dates shown to the right of each point are the dates of the last successful calibration. Select ZERO. The screen will change to: PLEASE WAIT Stabilising 000% © 2017 Aquaread Ltd. www.aquaread.com Page 58 of 143 ®...
Page 59: Verifying The Zero Calibration
20 or 1000, dependant upon the solution the probe is in. Wait while the Meter stabilises and calibrates. After successful calibration, the ‘Calibrating 100%’ screen will be displayed along with the Calibration Report, which will show the voltage output from the Turbidity Receiver Electrode © 2017 Aquaread Ltd. www.aquaread.com Page 59 of 143...
Page 60: Errors During Calibration
 Turbidity Science, Michael J. Sadar, Hach Company 1998.  Guidelines and Standard Procedures for continuous Water-Quality Monitors: Site Selection, Field Operation, Calibration, Record Computation and Reporting, Richard J. Wagner et al., USGS Reston VA Meeting, 2000. © 2017 Aquaread Ltd. www.aquaread.com Page 60 of 143...
Page 61: 7000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread Ltd. www.aquaread.com Page 61 of 143...
Page 62: Calibration Solution Preparation
100μg/L. This solution can now be used as Pt-2 calibration of the BGA-PC sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread Ltd. www.aquaread.com Page 62 of 143...
Page 63: Zero Point Calibration
Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. Select ZERO. The screen will change to: © 2017 Aquaread Ltd. www.aquaread.com Page 63 of 143...
Page 64: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2017 Aquaread Ltd. www.aquaread.com Page 64 of 143 ®...
Page 65: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 65 of 143 ®...
Page 66: 7000-Bga-Pe Saltwater Blue-Green Algae ( Phycoerythrin ) Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread Ltd. www.aquaread.com Page 66 of 143...
Page 67: Calibration Solution Preparation
8μg/L. This solution can now be used as Pt-2 calibration of the BGA-PE sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread Ltd. www.aquaread.com Page 67 of 143...
Page 68: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 68 of 143 ®...
Page 69: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2017 Aquaread Ltd. www.aquaread.com Page 69 of 143 ®...
Page 70: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 70 of 143 ®...
Page 71: 7000-Cphyll Chlorophyll Electrode
Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2017 Aquaread Ltd. www.aquaread.com Page 71 of 143...
Page 72: Calibration Solution Preparation
500μg/L. This solution can now be used as Pt-2 calibration of the CPHYLL sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2017 Aquaread Ltd. www.aquaread.com Page 72 of 143...
Page 73: Zero Point Calibration
Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 73 of 143 ®...
Page 74: Calibrating Point 2
If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2017 Aquaread Ltd. www.aquaread.com Page 74 of 143 ®...
Page 75: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 75 of 143 ®...
Page 76: 7000-Rhod Rhodamine Electrode
This is exactly the same calibration solution that is recommended for calibration of the BGA-PC electrode. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Rhodamine WT standard is recommended: © 2017 Aquaread Ltd. www.aquaread.com Page 76 of 143...
Page 77: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2017 Aquaread Ltd. www.aquaread.com Page 77 of 143 ®...
Page 78 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2017 Aquaread Ltd. www.aquaread.com Page 78 of 143 ®...
Page 79: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 79 of 143 ®...
Page 80: 7000-Fscein Fluorescein Electrode
In order to 'calibrate' the FSCEIN electrode, a 100µg/L calibration solution of Fluorescein Dye should be used. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Fluorescein Dye is recommended: © 2017 Aquaread Ltd. www.aquaread.com Page 80 of 143...
Page 81: Serial Dilution
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2017 Aquaread Ltd. www.aquaread.com Page 81 of 143 ®...
Page 82 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2017 Aquaread Ltd. www.aquaread.com Page 82 of 143 ®...
Page 83: Calibrating Point 2
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 83 of 143 ®...
Page 84: 7000-Refoil Refined Oil Electrode
Differences in the fluorescent response between various types of oil. • Differences in the fluorescent response caused by temperature. • Differences in the fluorescent response caused by ambient light. • Interference caused by turbidity. • © 2017 Aquaread Ltd. www.aquaread.com Page 84 of 143 ®...
Page 85: Special Precautions When Using The Refoil Electrode
Be sure to handle chemicals with care and to read and comply with all health and safety advice. 15.10.6. Serial Dilution 10ppm Napthalene salt can be prepared either as a one or two step process dependent upon the accuracy of the scales used. © 2017 Aquaread Ltd. www.aquaread.com Page 85 of 143 ®...
Page 86: Zero Point Calibration
4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2017 Aquaread Ltd. www.aquaread.com Page 86 of 143 ®...
Page 87: Calibrating Point 2
15.10.8. Calibrating Point 2 Remove the Probe from the calibration cup, shake off any excess water then dry the outer sleeve with a soft cloth. © 2017 Aquaread Ltd. www.aquaread.com Page 87 of 143 ®...
Page 88: Calculating And Applying A Grab Sample Factor
10 for error message handling. 15.10.11. Lens and Sleeve Maintenance On a daily basis, the lenses on the electrode should be wiped over with a soft damp cloth. © 2017 Aquaread Ltd. www.aquaread.com Page 88 of 143 ®...
Page 89 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 89 of 143 ®...
Page 90: 7000-Cdom/Fdom C
Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing concentrations. © 2017 Aquaread Ltd. www.aquaread.com Page 90 of 143...
Page 91: Calibrating The Cdom Electrode
In order to 'calibrate' the CDOM electrode, a 100ppb solution of Quinine Sulphate in sulphuric acid can be used. However, since Quinine Sulphate is extremely expensive and sulphuric acid is dangerous to handle, Aquaread Ltd has formulated an equivalent, non toxic standard for use during CDOM electrode calibration. This is available in 600mL bottles.
Page 92 Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. © 2017 Aquaread Ltd. www.aquaread.com Page 92 of 143 ®...
Page 93: Calibrating Point 2
100 gives a GS Factor of 1.25. This value should now be input on the bottom line of the electrode's calibration screen. Once the GS Factor value has been input, the OK key should be hit to send the Factor to © 2017 Aquaread Ltd. www.aquaread.com Page 93 of 143 ®...
Page 94: Errors During Calibration
The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2017 Aquaread Ltd. www.aquaread.com Page 94 of 143 ®...
Page 95: Optional Ise Electrodes Calibration And Maintenance
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2017 Aquaread Ltd. www.aquaread.com Page 95 of 143 ®...
Page 96: 7000-Amm Ammonium/Ammonia Electrode
1. The AP-7000's sleeve and wiper should also be removed in order to reduce the Probe's thermal mass. To calibrate the ISE electrode follow these steps: © 2017 Aquaread Ltd. www.aquaread.com Page 96 of 143...
Page 97 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: © 2017 Aquaread Ltd. www.aquaread.com Page 97 of 143 ®...
Page 98: Point 2
4. Referring to steps 4-7 above, select Pt-3 and press OK. The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. © 2017 Aquaread Ltd. www.aquaread.com Page 98 of 143 ®...
Page 99: Two-Point Calibration
1 only. 16.4.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread Ltd. www.aquaread.com Page 99 of 143 ®...
Page 100: 7000-Nit Nitrate Electrode
During three-point calibration, the AP-7000 and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2017 Aquaread Ltd. www.aquaread.com Page 100 of 143 ®...
Page 101 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 101 of 143 ®...
Page 102: Point 2
100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. The Meter will wait until the readings are stable, then it will send the calibration command © 2017 Aquaread Ltd. www.aquaread.com Page 102 of 143 ®...
Page 103: Two-Point Calibration
1 only. 16.5.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread Ltd. www.aquaread.com Page 103 of 143 ®...
Page 104: 7000-Chl Chloride Electrode
During three-point calibration, the AP-7000 and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2017 Aquaread Ltd. www.aquaread.com Page 104 of 143 ®...
Page 105 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 105 of 143 ®...
Page 106: Point 2
3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2017 Aquaread Ltd. www.aquaread.com Page 106 of 143 ®...
Page 107: Two-Point Calibration
1 only. 16.6.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread Ltd. www.aquaread.com Page 107 of 143 ®...
Page 108: 7000-Cal Calcium Electrode
During three-point calibration, the AP-7000 and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2017 Aquaread Ltd. www.aquaread.com Page 108 of 143 ®...
Page 109 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2017 Aquaread Ltd. www.aquaread.com Page 109 of 143 ®...
Page 110: Point 2
3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2017 Aquaread Ltd. www.aquaread.com Page 110 of 143 ®...
Page 111: Two-Point Calibration
1 only. 16.7.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread Ltd. www.aquaread.com Page 111 of 143 ®...
Page 112: 7000-Flu Fluoride Electrode
10ºC cooler. In order to achieve this, one batch of the 0.5ppm solution should be put into a refrigerator and the other two solutions should be put into a water bath at 25ºC. © 2017 Aquaread Ltd. www.aquaread.com Page 112 of 143...
Page 113: Three-Point Calibration
Pt-2? [01/Jan/17] Pt-3? [01/Jan/17] Calibration point 1 (Pt-1) is the warm 0.5ppm point. Calibration point 2 (Pt-2) is the warm 5ppm point. Calibration point 3 (Pt-3) is the cool 0.5ppm point. © 2017 Aquaread Ltd. www.aquaread.com Page 113 of 143...
Page 114: Point 3
3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 5ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2017 Aquaread Ltd. www.aquaread.com Page 114 of 143 ®...
Page 115: Two-Point Calibration
1 only. 16.8.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2017 Aquaread Ltd. www.aquaread.com Page 115 of 143 ®...
Page 116: Aqualink Pc Software
Windows ® on a stand-alone PC with a minimum screen resolution of 1024 x 768 and an available USB 2.0 socket. 17.1. Downloading AquaLink PC Software from the Aquaread ® website ™ The AquaLink PC Software is available for download using the following link: ™...
Page 117: Uploading Data From Your Aquameter
On the right of the screen, the position at which the data was logged is displayed in the GPS boxes (when logged using an AM-200 GPS Aquameter ® only). Latitude and longitude © 2017 Aquaread Ltd. www.aquaread.com Page 117 of 143...
Page 118: O Nscreen Help
Once a set of logged data has been uploaded from the Aquameter ® , it can be saved on your PC as a Raw Data file. These files use a proprietary Aquaread ® format and are saved with a .amf (aquameter file) extension.
Page 119 , Microsoft ® WordPad is the preferred text editor for viewing AquaLink Text Reports as this handles text file formatting better than Microsoft ® Notepad. A typical report cover page follows. © 2017 Aquaread Ltd. www.aquaread.com Page 119 of 143 ®...
Page 120: Typical Text Report Cover Page
Blocks of individual readings, laid out in chronological order, follow this cover page. The readings picked out on the cover page can be cross-referenced to the blocks of individual readings using the Tag numbers. © 2017 Aquaread Ltd. www.aquaread.com Page 120 of 143...
Page 121: Exporting Excel Files
Please note: Although you have downloaded the Google™ Earth application and are running it from your PC, you still need to be connected to the Internet in order for the application to access satellite images. A typical Google™ Earth image follows. © 2017 Aquaread Ltd. www.aquaread.com Page 121 of 143...
Page 122: Google™ Examples
If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo. © 2017 Aquaread Ltd. www.aquaread.com Page 122 of 143 ®...
Page 123: Limited Warranty
Meter and Probe sockets, and damage caused by leaking batteries are not covered. If service is required, contact our Service Department directly by email in the first instance (service@aquaread.com). Report the model number, date of purchase, serial number and problem. You will be given a Returns Authorisation number by our Service Department.
Page 124: Decontamination Certificate
Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________ Decontamination Procedure: _______________________________________________________________________ _______________________________________________________________________________________________ Certified by (print name) : ___________________________________ Title: __________________________________ Date: ______________________________________ Signature: __________________________________ © 2017 Aquaread Ltd. www.aquaread.com Page 124 of 143 ®...
Page 125: Troubleshooting
This section details some of the common difficulties you may encounter when using the Aquameter ® , Aquaprobe ® and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@aquaread.com). Problem Cause / Remedy The Aquameter ® will not ...
Page 126: Declaration Of Conformity
 20. DECLARATION OF CONFORMITY Aquaread ® Ltd declares that the equipment described herein is in compliance with the essential requirements and other relevant provisions of Directives 2004/108/EC and 1999/5/EC. © 2017 Aquaread Ltd. www.aquaread.com Page 126 of 143 ®...
Page 127: Appendix 1. The Tech Behind Aquaread's Optical Do Measurement System
Aquaprobe AP-7000 Instruction Manual 10401-00894 Rev M ® 21. Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System 21.1. Principle of Operation The Aquaread ® AquaPlus™ Optical DO measurement system works on the principle of Dynamic Luminescence Quenching. A gas-permeable chemical known as a luminophore is excited with short bursts of blue light, which causes molecules in the luminophore to emit red photons.
Page 128: Sensor Cap Life
The combination of low duty cycle and variable excitation brightness can stretch the useful life of a sensor cap as far as several years. © 2017 Aquaread Ltd. www.aquaread.com Page 128 of 143 ®...
Page 129: Appendix 2. Fitting Aux Electrodes
ENSURE NO GREASE IS APPLIED TO THE GOLD CONTACTS. Using a clean cloth or tissue paper, polish the gold contacts ensuring they are completely clean. Carefully insert the electrode into the AUX socket and tighten firmly until the O-ring is completely compressed. © 2017 Aquaread Ltd. www.aquaread.com Page 129 of 143...
Page 130: Socket Assignment And Calibration
Keep the blanking plug in a safe place. If you subsequently remove an electrode, be sure to replace the blanking plug and set the socket assignment back to EMPTY. © 2017 Aquaread Ltd. www.aquaread.com Page 130 of 143...
Page 131: Appendix 3. Standard Electrodes Detailed Specification
For this reason, when using the standard pH/ORP electrode, the water under test must have a minimum EC (electrical conductivity) of 100µS/cm. Special low EC pH electrodes are available to special order. © 2017 Aquaread Ltd. www.aquaread.com Page 131 of 143 ®...
Page 132: Appendix 4. Optical Electrodes Detailed Specification And Faqs
Gas or Liquid Chromatography in a laboratory although if grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen in order to improve the accuracy of future readings. © 2017 Aquaread Ltd. www.aquaread.com Page 132 of 143 ®...
Page 133: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why
2. MLR (Minimum Level of Repeatability) is the value below which optical electrode readings become generally unreliable and unrepeatable (unless taken under ideal conditions) due to interfering factors such as refraction from visible air bubbles and microscopic aeration. © 2017 Aquaread Ltd. www.aquaread.com Page 133 of 143 ®...
Page 134: What Is The Accuracy Of The Optical Electrodes
If grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen of each fluorescent type electrode in order to improve the accuracy of future readings. © 2017 Aquaread Ltd. www.aquaread.com Page 134 of 143...
Page 135: Appendix 4. Ise Electrodes Detailed Specification
All ion selective electrodes exhibit calibration drift over time. Drift should not be a major problem where the electrodes can be frequently calibrated. However, if the electrodes are to be used in long-term deployment studies, drift is almost certain to occur. © 2017 Aquaread Ltd. www.aquaread.com Page 135 of 143...
Page 136: Special Notes Concerning Ise Electrodes And Ph Buffer Solutions
Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2017 Aquaread Ltd. www.aquaread.com Page 136 of 143 ®...
Page 137: Appendix 7. Troubleshooting Turbidity
Never use an abrasive cleaner on the inside of the Probe sleeve or cap as they have been treated with a non-reflective coating which can be easily damaged. © 2017 Aquaread Ltd. www.aquaread.com Page 137 of 143 ®...
Page 138: About Turbidity Measurement
Similarly, if the turbidity electrode was subject to stray reflections from the inside of the calibration bottle during calibration, the absence of those reflections during deployment will result in the sensor seeing less reflected signal and therefore a negative reading will be displayed. © 2017 Aquaread Ltd. www.aquaread.com Page 138 of 143...
Page 139: Aeration
In order to avoid erroneous zero point calibration due to reflection it is important to zero the turbidity electrode in a non-reflective calibration bottle or in a vessel who's sides come no nearer than 10mm to the probe sleeve. © 2017 Aquaread Ltd. www.aquaread.com Page 139 of 143 ®...
Page 140: Sensor Saturation
➢ Always calibrate and zero the electrode as close to your sample temperature as possible. ➢ Always zero the optical electrodes just prior to use in clean water (bottled still mineral water is ideal) then deploy without disturbing the measurement chamber. © 2017 Aquaread Ltd. www.aquaread.com Page 140 of 143...
Page 141: References
10401-00894 Rev M ® 26.9. References This section is based upon Aquaread's experience in the field and information from the following sources. ➢ National Field Manual For the Collection of Water-Quality Data, Turbidity section 6.7, Revised by Chauncey W. Anderson, USGS, 2004.
Page 142: Appendix 8. Flow Through Cell
Tip: use the depth measurement feature of the AP-7000 to measure the operating pressure. At 500mb, the AP-7000 will show a depth of approximately 5 meters. © 2017 Aquaread Ltd. www.aquaread.com Page 142 of 143...
Page 143: Cleaning
Reduce flow rate to avoid cavitation. abnormally high or are fluctuating wildly. ✔ Optical electrode not zeroed in the Flowcell. Fill the Flowcell with clean water and Zero the optical electrode. © 2017 Aquaread Ltd. www.aquaread.com Page 143 of 143 ®...

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