Aquaread Aquaprobe AP-6000 Instruction Manual

Aquaread Aquaprobe AP-6000 Instruction Manual

Multiparameter water quality probe
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Instruction Manual
for the
Aquaprobe
®
AP-6000 & AP-7000Mk2
Multiparameter Water Quality Probe
and associated
Aquameter
®
, Utilities & Accessories
Aquameter
®
firmware Version 6.20 and Above
®
Aquaprobe
firmware Version 5.10 and Above
Document No. 10401-01668
Revision: B
Date: May 11, 2021
Aquaread
®
Ltd
Bridge House
Northdown Industrial Park
Broadstairs
Kent
CT10 3JP
ENGLAND
Phone: +44 1843 600 030
www.aquaread.com

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Summary of Contents for Aquaread Aquaprobe AP-6000

  • Page 1 Aquameter ® firmware Version 6.20 and Above ® Aquaprobe firmware Version 5.10 and Above Document No. 10401-01668 Revision: B Date: May 11, 2021 Aquaread ® Bridge House Northdown Industrial Park Broadstairs Kent CT10 3JP ENGLAND Phone: +44 1843 600 030...
  • Page 2 Notwithstanding the foregoing, in no event shall Aquaread ® Ltd and/or its affiliates aggregate liability arising under or in connection with Aquaread ® products, regardless of the number of events, occurrences, or claims giving rise to liability, be in excess of the price paid by the purchaser for the Aquaread ®...
  • Page 3: Table Of Contents

    ESSAGES 10.5. R ......................32 ESETTING TO ACTORY ALIBRATION EFAULTS 10.6. C ..............................32 ALIBRATION EPORTS 10.7. C .......................32 ALIBRATION TORAGE AND ETRIEVAL 11. AFTER USE..................................34 12. THE AQUAPROBE® AUTOMATIC CLEANING SYSTEM................35 © 2021 Aquaread Ltd. www.aquaread.com Page 3 of 145 ®...
  • Page 4 15.5.7. Calibrating Point 2............................65 15.5.8. Calculating and Applying a Grab Sample Factor..................65 15.5.9. Errors During Calibration..........................66 15.5.10. Lens and Sleeve Maintenance........................66 15.6. 6000/7000-BGA-PE S ...........67 ALTWATER REEN LGAE PHYCOERYTHRIN LECTRODE © 2021 Aquaread Ltd. www.aquaread.com Page 4 of 145 ®...
  • Page 5 .........91 HROMOPHORIC LUORESCENT ISSOLVED RGANIC ATTER 15.11.1. Principle of Operation..........................91 15.11.2. Limitations of Use............................91 15.11.3. Calibrating the CDOM Electrode........................92 15.11.4. Calibration Solutions...........................92 15.11.5. Zero Point Calibration..........................92 15.11.6. Calibrating Point 2............................94 © 2021 Aquaread Ltd. www.aquaread.com Page 5 of 145 ®...
  • Page 6 17.6. D GPS C ..........................118 ISPLAYING ORDINATES 17.7. O ..............................119 CREEN 17.8. S .............................119 AVING OGGED 17.9. R ............................119 ETRIEVING OGGED 17.10. E ..............................119 XPORTING 17.11. E ............................119 XPORTING EPORTS © 2021 Aquaread Ltd. www.aquaread.com Page 6 of 145 ®...
  • Page 7 RIOR ETURN 18.2. D ..........................125 ECONTAMINATION ERTIFICATE 19. TROUBLESHOOTING.............................126 20. DECLARATION OF CONFORMITY........................128 21. APPENDIX 1. THE TECH BEHIND AQUAREAD’S OPTICAL DO MEASUREMENT SYSTEM....129 21.1. P ............................129 RINCIPLE OF PERATION 21.2. S ..............................130 ENSOR 22. APPENDIX 2. FITTING AUX ELECTRODES.....................131 22.1.
  • Page 8: Introduction

    If your Aquameter ® is running earlier firmware, the functionality may differ from that shown in this manual. In this case, contact Aquaread for an earlier version of this manual or return your equipment for firmware upgrade. 2. What’s in the Box? The Aquameter ®...
  • Page 9: About The Probe Sleeve And Sleeve End Cap

    Probe’s optical and EC measurement system, and MUST be fitted during calibration and measurement for correct operation. All Aquaread® Optical Electrodes are incredibly sensitive. For example, the Turbidity electrode is capable of measuring between 0 and 3000NTU with an internal resolution of greater than 0.1NTU.
  • Page 10: Top Tips For Successful Measurements Using Optical Electrodes

    Drift should not be a major problem where the electrodes can be frequently calibrated. Also remember, in order to achieve accurate readings with ISE electrodes, the Probe needs to be placed in flowing water with a minimum flow rate of 0.3m/s over the electrode. © 2021 Aquaread Ltd. www.aquaread.com Page 10 of 145 ®...
  • Page 11: Important Notes Regarding Galvanic Corrosion

    ® undamaged. Sacrificial anodes are, as the name suggest, sacrificial, so will need replacing periodically. Sacrificial anodes are available for the AP-6000 and AP-7000. Please contact your Aquaread dealer and order part number SA-7000. © 2021 Aquaread Ltd. www.aquaread.com Page 11 of 145...
  • Page 12: Battery Installation And Care

    After a further 15 seconds the backlight will switch off. During normal operation, if you want to activate the backlight without changing the Meter function, simply press the ESC key. © 2021 Aquaread Ltd. www.aquaread.com Page 12 of 145...
  • Page 13: Overview Of The Operating System

    When the time and date are correct, press the OK key. Don’t worry if you make a mistake first time round. You can easily get back to these screens later through the MENU key. © 2021 Aquaread Ltd. www.aquaread.com Page 13 of 145 ®...
  • Page 14: Connecting An Aquaprobe

    , align the white dot on the Aquaprobe ® with the ◄AQUAREAD logo on the plug body, then press the plug into the socket and tighten the retaining collar fully. Once the Probe has been connected to the Extension Cable, the Aquameter ®...
  • Page 15: Taking Measurements

    EC:6541µS/cm   TDS:3271mg/L GPS:3D Pos   SAL:03.57 PSU    SSG:01.3σt  GPS:3D Pos    BARO:1013mb DEPTH:01.75m Hit [OK] to zero GPS:3D Pos   © 2021 Aquaread Ltd. www.aquaread.com Page 15 of 145 ®...
  • Page 16: What Does It All Mean

    In addition to the individual trend indications, there is a global stability indication, which is displayed when all readings are stable. This takes the form of a flashing double headed arrow which is displayed at the start of the third line of the display. © 2021 Aquaread Ltd. www.aquaread.com Page 16 of 145 ®...
  • Page 17: Temperature Compensation

    (usually 20ºC or 25ºC) although a variation of up to +/-10ºC makes very little difference in reality. During pH measurement, temperature variation is automatically compensated for. © 2021 Aquaread Ltd. www.aquaread.com Page 17 of 145 ®...
  • Page 18: Gps Reception

    ® (AM-200) contains a built-in GPS/GLONAS receiver and antenna. The antenna is situated at the top of the case, just behind the AQUAREAD Logo. For optimum signal reception, the antenna must be able to ‘see’ a reasonably large amount of the sky. The GPS receiver will not work indoors or when shielded from the sky by any solid structure.
  • Page 19: Depth Measurement

    The depth sensor is automatically re-zeroed each time the DO 100% point is calibrated in free air. The depth sensor is factory calibrated at two temperatures and two pressures. No further calibration is required for the lifetime of the sensor. © 2021 Aquaread Ltd. www.aquaread.com Page 19 of 145 ®...
  • Page 20: Memory Mode

    Using the up/down keys, select the electrode you are interested in, then press either the OK key or the right arrow key. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. © 2021 Aquaread Ltd. www.aquaread.com Page 20 of 145...
  • Page 21: Clearing The Memory

    Calibration Setup & Install Select Auto Data Logging by pressing the down arrow key then the right arrow key or the OK key. The Auto Data Logging screen will be displayed. © 2021 Aquaread Ltd. www.aquaread.com Page 21 of 145...
  • Page 22: Battery And Memory Duration In Low Power Logging Mode

    5 mins 8 Days 34 Days 1 min 45 Hours 6.9 Days So, it can be seen that although the Meter has a maximum data capacity of 625 days, for © 2021 Aquaread Ltd. www.aquaread.com Page 22 of 145 ®...
  • Page 23: Important Information About Memory Mode

    The same rules apply when data is output to a PC running AquaLink Software via the USB cable. The data that is output is always as per the Meter’s current configuration. You can output the data as many times as you like in various Meter configurations. © 2021 Aquaread Ltd. www.aquaread.com Page 23 of 145 ®...
  • Page 24: Setup & Install

    Finally, this screen allows you to set the factor that the Meter uses to calculate Total Dissolved Solids from Specific EC. This is the TDS Fact: (TDS = EC x TDS Fact) and can be set anywhere between 0.00 and 1.00. Default value is 0.65. © 2021 Aquaread Ltd. www.aquaread.com Page 24 of 145 ®...
  • Page 25: Aux Socket Assignment

    The Socket Assignment option is only available if the Aquameter ® is connected to an Aquaprobe®. This is because the assignment data is held in the Aquaprobe ® , not in the Aquameter ® © 2021 Aquaread Ltd. www.aquaread.com Page 25 of 145 ®...
  • Page 26 Please note: changing an AUX Socket assignment will clear all the calibration data for that socket. If you subsequently remove an electrode, be sure to set the socket assignment back to EMPTY. © 2021 Aquaread Ltd. www.aquaread.com Page 26 of 145...
  • Page 27: Rapidcal Calibration Method

    The caps should be fitted to all ISE Electrodes during when using RapidCal order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2021 Aquaread Ltd. www.aquaread.com Page 27 of 145 ®...
  • Page 28: Using Rapidcal

    7. Select RapidCal. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until all readings are stable, then it will send the RapidCal command to the Probe, where the calibration takes place. © 2021 Aquaread Ltd. www.aquaread.com Page 28 of 145 ®...
  • Page 29: To Check / Calibrate The 100% Saturation Point In Damp Air

    Finally, if you have any optical electrodes fitted, you should calibrate the zero points now using fresh, still mineral water. Refer to the relevant sections in this manual for advice on zero point calibration of your optical electrodes. © 2021 Aquaread Ltd. www.aquaread.com Page 29 of 145 ®...
  • Page 30: Calibration Error Messages

    If you try to calibrate any of the optical electrodes or the EC without the sleeve, end cap and wiper fitted, you may get an error message. © 2021 Aquaread Ltd. www.aquaread.com Page 30 of 145...
  • Page 31: Resetting To Factory Calibration Defaults

    Meter, this data is transferred for display and logging. This is a major advantage and allows you to use a variety of different Probes with a single Meter, without the need for re-calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 31 of 145...
  • Page 32 Pushing the left or right arrow keys will toggle this screen with the calibration report screen. PH7 Offset:+01.2mV PH4 Slope:56.8mV/pH PH10 Slope:56.3mV/pH ORP Offset:-02.6mV The calibration report screen displays the calibration report values from the last calibration. To exit this screen press the ESC key. © 2021 Aquaread Ltd. www.aquaread.com Page 32 of 145 ®...
  • Page 33: After Use

    TIP: Occasional application of a smear of silicone grease (provided) to the connector O- rings and thread, Sleeve thread, the protective Sleeve End Cap thread will make fitting and removal of these parts easier. © 2021 Aquaread Ltd. www.aquaread.com Page 33 of 145...
  • Page 34: The Aquaprobe® Automatic Cleaning System

    When connected to an AquaLogger, the cleaning rate can be set in the LoggerLink software. When connected to a third party telemetry device via an Aquaread® BlackBox, the BlackBox will send a clean command every six hours. Please note, power must be left permanently connected to the BlackBox in order for this feature to work correctly.
  • Page 35 Next, slide the brush holder off the central shaft. Next, slide the wiper arm out of the brush holder. Next, slide the top brush out of the wiper arm. © 2021 Aquaread Ltd. www.aquaread.com Page 35 of 145 ®...
  • Page 36 When the brush holder has been re-fitted and the spring clip is in place, rotate the • brush holder manually until you feel the drive clutch engage. Activate the cleaning system after re-assembly to ensure correct operation. • © 2021 Aquaread Ltd. www.aquaread.com Page 36 of 145 ®...
  • Page 37: Wiper Motor Removal And Replacement

    Includes AP/AS-6000 brush holder, one set of brushes and one 6000- Brush Holder spring clip. 7000Mk2-Brush Includes AP/AS-7000 brush holder, one set of brushes and one Holder spring clip. © 2021 Aquaread Ltd. www.aquaread.com Page 37 of 145 ®...
  • Page 38: Important Notes Regarding Long-Term Deployment

    , especially for a long term deployment, it is important to recognise and avoid the possibility of creating a situation where galvanic corrosion can occur. See section 2.5.1 Important Notes Regarding Galvanic Corrosion for more details. © 2021 Aquaread Ltd. www.aquaread.com Page 38 of 145 ®...
  • Page 39: Ph/Orp Electrode Calibration And Maintenance

    Electrode Storage Solution each time it is removed and replaced. If a pH/ORP electrode is inadvertently allowed to dry out, it must be re-hydrated by soaking in storage solution for at least one hour prior to use. © 2021 Aquaread Ltd. www.aquaread.com Page 39 of 145 ®...
  • Page 40: Calibrating Ph

    3. Wait until the temperature and pH measurements are completely stable. 4. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF – 104ºF). 5. Press the MENU key then select Calibration. The following screen will be displayed. © 2021 Aquaread Ltd. www.aquaread.com Page 40 of 145 ®...
  • Page 41 This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2021 Aquaread Ltd. www.aquaread.com Page 41 of 145...
  • Page 42: Calibrating The Second Point

    85%, ‘ERROR 01’ will be flashed on the bottom line of the display. If this occurs, or if the pH reading response becomes slow, recondition the electrode as described below. © 2021 Aquaread Ltd. www.aquaread.com Page 42 of 145...
  • Page 43: Servicing The Ph Electrode

    Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2021 Aquaread Ltd. www.aquaread.com Page 43 of 145 ®...
  • Page 44 Remove the Probe from the calibration cup, rinse thoroughly in fresh water, shake off any excess and dry the outer sleeve with a soft cloth. Dampen the sponge in the storage cap with storage solution and fit it to the pH/ORP electrode. ORP calibration is now complete. © 2021 Aquaread Ltd. www.aquaread.com Page 44 of 145 ®...
  • Page 45: Converting Orp Readings To The Hydrogen Scale

    The ORP reference electrode used in Aquaread ® combination electrodes is a 3MPK1 silver...
  • Page 46: Do/Ec Electrode Calibration And Maintenance

    The Aquaprobe® features an optical DO sensor. This sensor does not use a liquid electrolyte and has a black rubber gas-permeable membrane. See Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System for further details. 14.3. Precautions During Use EC measurement is not possible with the Probe central wiper removed as the central wiper forms an integral part of the measurement system.
  • Page 47 This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2021 Aquaread Ltd. www.aquaread.com Page 47 of 145...
  • Page 48: Calibrating The Do 100% Saturation Point In Moist Air

    2. Unscrew the Optical DO Cap from the end of the DO/EC electrode by rotating it anti- clockwise. Do not touch the exposed optical components. 3. Apply a light smear of silicone grease to the thread and O ring. © 2021 Aquaread Ltd. www.aquaread.com Page 48 of 145...
  • Page 49 5. Carry out both Zero point and 100% point DO calibration as described earlier. Please Note: It is essential when replacing the Optical DO Cap to calibrate the Zero point BEFORE calibrating the 100% point. © 2021 Aquaread Ltd. www.aquaread.com Page 49 of 145 ®...
  • Page 50: Calibrating Ec

    ® dealers or can be easily manufactured by adding 33.03 grams of laboratory grade sodium chloride (99.9% pure) to 1 litre of deionised water. RapidCal is available from all Aquaread ® dealers. The Probe's central wiper forms an integral, working part of the Probe’s EC measurement system, and MUST be fitted during calibration and measurement for correct operation.
  • Page 51 The screen will change to: PLEASE WAIT Stabilising 000% If the calibration is successful, the counter will reach 100% and the following screen will be displayed. Cell constant:0.98 Calibrating 100% Press [OK] © 2021 Aquaread Ltd. www.aquaread.com Page 51 of 145 ®...
  • Page 52: Verifying Ec Calibration

    DO/EC electrode with a soft cloth or toothbrush and non-abrasive detergent. Never use solvent or alcohol based products to clean the DO/EC electrode. After cleaning, replace the Probe sleeve and re-calibrate. © 2021 Aquaread Ltd. www.aquaread.com Page 52 of 145...
  • Page 53: Calibrating Temperature

    If the offset has been correctly input, the Aquameter ® will now be reading the corrected temperature. The temperature correction offset is stored in the Aquaprobe ® and applied at all times going forward. © 2021 Aquaread Ltd. www.aquaread.com Page 53 of 145 ®...
  • Page 54: Optional Optical Electrodes Calibration And Maintenance

    Probe’s optical and EC measurement system, and MUST be fitted during calibration and measurement for correct operation. All Aquaread® Optical Electrodes are incredibly sensitive. For example, the Turbidity electrode is capable of measuring between 0 and 3000NTU with an internal resolution of greater than 0.1NTU.
  • Page 55: Top Tips For Successful Measurements Using Optical Electrodes

    If you attempt to calibrate an upper calibration point without first calibrating the ZERO point, a calibration error will occur. 15.3. Fluorescent Electrode Grab Sample Correction Factor A unique feature of the Aquaread ® fluorescent type electrodes is the ability to include a correction factor based upon a grab sample.
  • Page 56: 6000/7000-Turb Turbidity Electrode

    Sleeve End Cap fitted. If you experience any problems using the Turbidity Electrode, refer to Appendix 7. Troubleshooting Turbidity © 2021 Aquaread Ltd. www.aquaread.com Page 56 of 145 ®...
  • Page 57: Negative Turbidity Readings

    A three point calibration should be carried out once a month to ensure optimum accuracy. Zeroing of the Turbidity electrode is normally carried out automatically during RapidCal (see RapidCal Calibration Method). © 2021 Aquaread Ltd. www.aquaread.com Page 57 of 145 ®...
  • Page 58: Turbidity Zero Point Calibration

    1000? [01/Jan/14] [01/Jan/14] The dates shown to the right of each point are the dates of the last successful calibration. Select ZERO. The screen will change to: PLEASE WAIT Stabilising 000% © 2021 Aquaread Ltd. www.aquaread.com Page 58 of 145 ®...
  • Page 59: Verifying The Zero Calibration

    20 or 1000, dependant upon the solution the probe is in. Wait while the Meter stabilises and calibrates. After successful calibration, the ‘Calibrating 100%’ screen will be displayed along with the Calibration Report, which will show the voltage output from the Turbidity Receiver Electrode © 2021 Aquaread Ltd. www.aquaread.com Page 59 of 145...
  • Page 60: Errors During Calibration

     Turbidity Science, Michael J. Sadar, Hach Company 1998.  Guidelines and Standard Procedures for continuous Water-Quality Monitors: Site Selection, Field Operation, Calibration, Record Computation and Reporting, Richard J. Wagner et al., USGS Reston VA Meeting, 2000. © 2021 Aquaread Ltd. www.aquaread.com Page 60 of 145...
  • Page 61: 6000/7000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode

    Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2021 Aquaread Ltd. www.aquaread.com Page 61 of 145...
  • Page 62: Calibration Solution Preparation

    100μg/L. This solution can now be used as Pt-2 calibration of the BGA-PC sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2021 Aquaread Ltd. www.aquaread.com Page 62 of 145...
  • Page 63: Zero Point Calibration

    Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. Select ZERO. The screen will change to: © 2021 Aquaread Ltd. www.aquaread.com Page 63 of 145...
  • Page 64: Calibrating Point 2

    If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2021 Aquaread Ltd. www.aquaread.com Page 64 of 145 ®...
  • Page 65: Lens And Sleeve Maintenance

    The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 65 of 145 ®...
  • Page 66 Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2021 Aquaread Ltd. www.aquaread.com Page 66 of 145...
  • Page 67 8μg/L. This solution can now be used as Pt-2 calibration of the BGA-PE sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2021 Aquaread Ltd. www.aquaread.com Page 67 of 145...
  • Page 68 Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 68 of 145 ®...
  • Page 69 If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2021 Aquaread Ltd. www.aquaread.com Page 69 of 145 ®...
  • Page 70 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 70 of 145 ®...
  • Page 71 Subsequently, a single point (Zero) calibration should be carried out daily. Full two-point calibration should be carried out every few months. © 2021 Aquaread Ltd. www.aquaread.com Page 71 of 145...
  • Page 72 500μg/L. This solution can now be used as Pt-2 calibration of the CPHYLL sensor. The dilute solution can be stored in a dark bottle in a refrigerator for up to five days. After that time it must be discarded. © 2021 Aquaread Ltd. www.aquaread.com Page 72 of 145...
  • Page 73 Pt-2? [01/Jan/17] GS Factor:01.00 Calibration point 2 (Pt-2) is the upper calibration point. The dates shown to the right of each point are the dates of the last successful calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 73 of 145 ®...
  • Page 74 If grab sample data is available for the location in which you plan to take measurements, you should calculate a GS Factor for the electrode and input it on the bottom line of the electrode's calibration screen. © 2021 Aquaread Ltd. www.aquaread.com Page 74 of 145 ®...
  • Page 75 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 75 of 145 ®...
  • Page 76 This is exactly the same calibration solution that is recommended for calibration of the BGA-PC electrode. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Rhodamine WT standard is recommended: © 2021 Aquaread Ltd. www.aquaread.com Page 76 of 145...
  • Page 77 3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2021 Aquaread Ltd. www.aquaread.com Page 77 of 145 ®...
  • Page 78 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2021 Aquaread Ltd. www.aquaread.com Page 78 of 145 ®...
  • Page 79 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 79 of 145 ®...
  • Page 80 In order to 'calibrate' the FSCEIN electrode, a 100µg/L calibration solution of Fluorescein Dye should be used. The 100µg/L calibration solution should be freshly prepared by serial dilution from 200g/L standard using deionised water. The following Fluorescein Dye is recommended: © 2021 Aquaread Ltd. www.aquaread.com Page 80 of 145...
  • Page 81 3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF - 104ºF). 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal © 2021 Aquaread Ltd. www.aquaread.com Page 81 of 145 ®...
  • Page 82 (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7 Calibration Data Storage and Retrieval. Press OK then ESC repeatedly to return to normal reading mode. © 2021 Aquaread Ltd. www.aquaread.com Page 82 of 145 ®...
  • Page 83 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 83 of 145 ®...
  • Page 84 Differences in the fluorescent response caused by temperature. • Differences in the fluorescent response caused by ambient light. • Interference caused by turbidity. • Fluorescence measurement techniques are ideal for researchers who are interested in © 2021 Aquaread Ltd. www.aquaread.com Page 84 of 145 ®...
  • Page 85 Be sure to handle chemicals with care and to read and comply with all health and safety advice. 15.10.6. Serial Dilution 10ppm Napthalene salt can be prepared either as a one or two step process dependent upon the accuracy of the scales used. © 2021 Aquaread Ltd. www.aquaread.com Page 85 of 145 ®...
  • Page 86 4. Press the MENU key then select Calibration. The following screen will be displayed. Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes © 2021 Aquaread Ltd. www.aquaread.com Page 86 of 145 ®...
  • Page 87 15.10.8. Calibrating Point 2 Remove the Probe from the calibration cup, shake off any excess water then dry the outer sleeve with a soft cloth. © 2021 Aquaread Ltd. www.aquaread.com Page 87 of 145 ®...
  • Page 88 10 for error message handling. 15.10.11. Lens and Sleeve Maintenance On a daily basis, the lenses on the electrode should be wiped over with a soft damp cloth. © 2021 Aquaread Ltd. www.aquaread.com Page 88 of 145 ®...
  • Page 89 The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses. © 2021 Aquaread Ltd. www.aquaread.com Page 89 of 145 ®...
  • Page 90 Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing concentrations. © 2021 Aquaread Ltd. www.aquaread.com Page 90 of 145...
  • Page 91 In order to 'calibrate' the CDOM electrode, a 100ppb solution of Quinine Sulphate in sulphuric acid can be used. However, since Quinine Sulphate is extremely expensive and sulphuric acid is dangerous to handle, Aquaread Ltd has formulated an equivalent, non toxic standard for use during CDOM electrode calibration. This is available in 600mL bottles.
  • Page 92 Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed. © 2021 Aquaread Ltd. www.aquaread.com Page 92 of 145 ®...
  • Page 93 This value should now be input on the bottom line of the electrode's calibration screen. Once the GS Factor value has been input, the OK key should be hit to send the Factor to the Probe. © 2021 Aquaread Ltd. www.aquaread.com Page 93 of 145 ®...
  • Page 94 In order to avoid this, do not leave the Aquaprobe sitting in CDOM solution for more time than it takes to do the actual calibration. As soon as the Point 2 calibration is complete, remove the Aquaprobe from the CDOM calibration solution and rinse thoroughly. © 2021 Aquaread Ltd. www.aquaread.com Page 94 of 145...
  • Page 95 Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2021 Aquaread Ltd. www.aquaread.com Page 95 of 145 ®...
  • Page 96 1. The Aquaprobe's sleeve and wiper should also be removed in order to reduce the Probe's thermal mass. To calibrate the ISE electrode follow these steps: © 2021 Aquaread Ltd. www.aquaread.com Page 96 of 145...
  • Page 97 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: © 2021 Aquaread Ltd. www.aquaread.com Page 97 of 145 ®...
  • Page 98 4. Referring to steps 4-7 above, select Pt-3 and press OK. The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. © 2021 Aquaread Ltd. www.aquaread.com Page 98 of 145 ®...
  • Page 99 1 only. 16.4.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2021 Aquaread Ltd. www.aquaread.com Page 99 of 145 ®...
  • Page 100 During three-point calibration, the Aquaprobe® and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2021 Aquaread Ltd. www.aquaread.com Page 100 of 145 ®...
  • Page 101 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 101 of 145 ®...
  • Page 102 100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. The Meter will wait until the readings are stable, then it will send the calibration command © 2021 Aquaread Ltd. www.aquaread.com Page 102 of 145 ®...
  • Page 103 1 only. 16.5.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2021 Aquaread Ltd. www.aquaread.com Page 103 of 145 ®...
  • Page 104 During three-point calibration, the Aquaprobe® and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2021 Aquaread Ltd. www.aquaread.com Page 104 of 145 ®...
  • Page 105 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 105 of 145 ®...
  • Page 106 3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2021 Aquaread Ltd. www.aquaread.com Page 106 of 145 ®...
  • Page 107 1 only. 16.6.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2021 Aquaread Ltd. www.aquaread.com Page 107 of 145 ®...
  • Page 108 During three-point calibration, the Aquaprobe® and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1. © 2021 Aquaread Ltd. www.aquaread.com Page 108 of 145 ®...
  • Page 109 Calibration point 1 (Pt-1) is the warm 10ppm point. Calibration point 2 (Pt-2) is the warm 100ppm point. Calibration point 3 (Pt-3) is the cool 10ppm point. The dates shown to the right of each point are the dates of the last successful calibration. © 2021 Aquaread Ltd. www.aquaread.com Page 109 of 145 ®...
  • Page 110 3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 100ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2021 Aquaread Ltd. www.aquaread.com Page 110 of 145 ®...
  • Page 111 1 only. 16.7.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2021 Aquaread Ltd. www.aquaread.com Page 111 of 145 ®...
  • Page 112 10ºC cooler. In order to achieve this, one batch of the 0.5ppm solution should be put into a refrigerator and the other two solutions should be put into a water bath at 25ºC. © 2021 Aquaread Ltd. www.aquaread.com Page 112 of 145...
  • Page 113 Pt-2? [01/Jan/17] Pt-3? [01/Jan/17] Calibration point 1 (Pt-1) is the warm 0.5ppm point. Calibration point 2 (Pt-2) is the warm 5ppm point. Calibration point 3 (Pt-3) is the cool 0.5ppm point. © 2021 Aquaread Ltd. www.aquaread.com Page 113 of 145...
  • Page 114 3. Ensure the temperature of the solution is at least 10ºC cooler than the previous 5ppm calibration point. If the solution is too warm, calibration will fail. 4. Referring to steps 4-7 above, select Pt-3 and press OK. © 2021 Aquaread Ltd. www.aquaread.com Page 114 of 145 ®...
  • Page 115 1 only. 16.8.5. Errors During Calibration If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. © 2021 Aquaread Ltd. www.aquaread.com Page 115 of 145 ®...
  • Page 116 Windows ® on a stand-alone PC with a minimum screen resolution of 1024 x 768 and an available USB 2.0 socket. 17.1. Downloading AquaLink PC Software from the Aquaread ® website ™ The AquaLink PC Software is available for download using the following link: ™...
  • Page 117 On the right of the screen, the position at which the data was logged is displayed in the GPS boxes (when logged using an AM-200 GPS Aquameter ® only). Latitude and longitude © 2021 Aquaread Ltd. www.aquaread.com Page 117 of 145...
  • Page 118 Once a set of logged data has been uploaded from the Aquameter ® , it can be saved on your PC as a Raw Data file. These files use a proprietary Aquaread ® format and are saved with a .amf (aquameter file) extension.
  • Page 119 , Microsoft ® WordPad is the preferred text editor for viewing AquaLink Text Reports as this handles text file formatting better than Microsoft ® Notepad. A typical report cover page follows. © 2021 Aquaread Ltd. www.aquaread.com Page 119 of 145 ®...
  • Page 120 Blocks of individual readings, laid out in chronological order, follow this cover page. The readings picked out on the cover page can be cross-referenced to the blocks of individual readings using the Tag numbers. © 2021 Aquaread Ltd. www.aquaread.com Page 120 of 145...
  • Page 121: Exporting Excel Files

    Please note: Although you have downloaded the Google™ Earth application and are running it from your PC, you still need to be connected to the Internet in order for the application to access satellite images. A typical Google™ Earth image follows. © 2021 Aquaread Ltd. www.aquaread.com Page 121 of 145...
  • Page 122: Google™ Examples

    If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo. © 2021 Aquaread Ltd. www.aquaread.com Page 122 of 145 ®...
  • Page 123: Limited Warranty

    Meter and Probe sockets, and damage caused by leaking batteries are not covered. If service is required, contact our Service Department directly by email in the first instance (service@aquaread.com). Report the model number, date of purchase, serial number and problem. You will be given a Returns Authorisation number by our Service Department.
  • Page 124: Decontamination Certificate

    Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________ Decontamination Procedure: _______________________________________________________________________ _______________________________________________________________________________________________ Certified by (print name) : ___________________________________ Title: __________________________________ Date: ______________________________________ Signature: __________________________________ © 2021 Aquaread Ltd. www.aquaread.com Page 124 of 145 ®...
  • Page 125: Troubleshooting

    This section details some of the common difficulties you may encounter when using the Aquameter ® , Aquaprobe ® and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@aquaread.com). Problem Cause / Remedy The Aquameter ® will not ...
  • Page 126 Thoroughly clean the Probe then re-zero in completely clean/clear water. Refer to Appendix 7. Troubleshooting Turbidity  © 2021 Aquaread Ltd. www.aquaread.com Page 126 of 145 ®...
  • Page 127: Declaration Of Conformity

    Aquaprobe AP-6000 & AP-7000Mk2 Instruction Manual 10401-01668 Rev B ® 20. DECLARATION OF CONFORMITY © 2021 Aquaread Ltd. www.aquaread.com Page 127 of 145 ®...
  • Page 128: Appendix 1. The Tech Behind Aquaread's Optical Do Measurement System

    Aquaprobe AP-6000 & AP-7000Mk2 Instruction Manual 10401-01668 Rev B ® 21. Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System 21.1. Principle of Operation The Aquaread ® AquaPlus™ Optical DO measurement system works on the principle of Dynamic Luminescence Quenching. A gas-permeable chemical known as a luminophore is excited with short bursts of blue light, which causes molecules in the luminophore to emit red photons.
  • Page 129: Sensor Cap Life

    The combination of low duty cycle and variable excitation brightness can stretch the useful life of a sensor cap as far as several years. © 2021 Aquaread Ltd. www.aquaread.com Page 129 of 145 ®...
  • Page 130 ENSURE NO GREASE IS APPLIED TO THE GOLD CONTACTS. Using a clean cloth or tissue paper, polish the gold contacts ensuring they are completely clean. Carefully insert the electrode into the AUX socket and tighten firmly until the O-ring is completely compressed. © 2021 Aquaread Ltd. www.aquaread.com Page 130 of 145...
  • Page 131: 6000/7000-Bga-Pe Saltwater

    Keep the blanking plug in a safe place. If you subsequently remove an electrode, be sure to replace the blanking plug and set the socket assignment back to EMPTY. © 2021 Aquaread Ltd. www.aquaread.com Page 131 of 145...
  • Page 132: Appendix 3. Standard Electrodes Detailed Specification

    For this reason, when using the standard pH/ORP electrode, the water under test must have a minimum EC (electrical conductivity) of 100µS/cm. Special low EC pH electrodes are available to special order. © 2021 Aquaread Ltd. www.aquaread.com Page 132 of 145 ®...
  • Page 133: Electrode

    Gas or Liquid Chromatography in a laboratory although i f grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen in order to improve the accuracy of future readings. © 2021 Aquaread Ltd. www.aquaread.com Page 133 of 145 ®...
  • Page 134: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why

    2. MLR (Minimum Level of Repeatability) is the value below which optical electrode readings become generally unreliable and unrepeatable (unless taken under ideal conditions) due to interfering factors such as refraction from visible air bubbles and microscopic aeration. © 2021 Aquaread Ltd. www.aquaread.com Page 134 of 145 ®...
  • Page 135 If grab sample data is available, a Grab Sample Factor (GS Factor) can be input on the calibration screen of each fluorescent type electrode in order to improve the accuracy of future readings. © 2021 Aquaread Ltd. www.aquaread.com Page 135 of 145...
  • Page 136: Appendix 4. Ise Electrodes Detailed Specification

    All ion selective electrodes exhibit calibration drift over time. Drift should not be a major problem where the electrodes can be frequently calibrated. However, if the electrodes are to be used in long-term deployment studies, drift is almost certain to occur. © 2021 Aquaread Ltd. www.aquaread.com Page 136 of 145...
  • Page 137 Electrodes during pH calibration or when using RapidCal in order to protect the ISE electrodes from the effects of the buffer solution. At all other times, the ISE electrodes should be left uncovered. © 2021 Aquaread Ltd. www.aquaread.com Page 137 of 145 ®...
  • Page 138: Lens And Sleeve Maintenance

    Never use an abrasive cleaner on the inside of the Probe sleeve or cap as they have been treated with a non-reflective coating which can be easily damaged. © 2021 Aquaread Ltd. www.aquaread.com Page 138 of 145 ®...
  • Page 139: About Turbidity Measurement

    Similarly, if the turbidity electrode was subject to stray reflections from the inside of the calibration bottle during calibration, the absence of those reflections during deployment will result in the sensor seeing less reflected signal and therefore a negative reading will be displayed. © 2021 Aquaread Ltd. www.aquaread.com Page 139 of 145...
  • Page 140: Aeration

    This situation is rare, but can occur if the sensor is sitting on or near the bottom in shallow water in direct sunlight where the sunlight can be reflected back into the Probe by light coloured sand or pebbles. © 2021 Aquaread Ltd. www.aquaread.com Page 140 of 145 ®...
  • Page 141: Top Tips For Successful Measurements Using Optical Electrodes

    Always calibrate and zero the electrode as close to your sample temperature as • possible. Always zero the optical electrodes just prior to use in clean water (bottled still • mineral water is ideal) then deploy without disturbing the measurement chamber. © 2021 Aquaread Ltd. www.aquaread.com Page 141 of 145 ®...
  • Page 142: References

    AP-6000 & AP-7000Mk2 Instruction Manual 10401-01668 Rev B ® 26.8. References This section is based upon Aquaread's experience in the field and information from the following sources. National Field Manual For the Collection of Water-Quality Data, Turbidity section 6.7, •...
  • Page 143: Appendix 8. Flow Through Cell

    The photograph on the far right shows the complete assembly. The Flowcell is designed to be used with the AP-6000 end cap removed. See Zeroing Optical Electrodes on the next page. © 2021 Aquaread Ltd. www.aquaread.com Page 143 of 145 ®...
  • Page 144: Ap-6000 Installation Into The Flowcell

    Aeration of sample water. Check all joints for air • high or are fluctuating wildly. leaks. Reduce flow rate to avoid cavitation. Turbidity or other optical Air bubbles adhering to the Turbidity Electrode • © 2021 Aquaread Ltd. www.aquaread.com Page 144 of 145 ®...
  • Page 145 Reduce flow rate to avoid cavitation. abnormally high or are fluctuating wildly. Optical electrode not zeroed in the Flowcell. Fill • the Flowcell with clean water and Zero the optical electrode. © 2021 Aquaread Ltd. www.aquaread.com Page 145 of 145 ®...

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