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HOMOGENIZING KITS
USER MANUAL

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Summary of Contents for Omni International TIP HOMOGENIZING KIT

  • Page 1 ™ HOMOGENIZING KITS USER MANUAL...
  • Page 2: Warranty Information

    WARRANTY INFORMATION This manual is a guide for the use of the Omni International’s Omni Tip™ Homogenizing Kits and accessories. Data herein has been verified and validated. It is believed adequate for the intended use of the instrument. If the instrument or procedures are used for...
  • Page 3 Do not attempt to modify any part of the Omni TH. If you experience problems with or have questions about your Omni TH, contact your authorized dealer or call Omni International at 1-800-776-4431 or 1-770-421-0058. DANGER -DO NOT allow the machine to be submerged in any liquid.
  • Page 4: Table Of Contents

    TABLE OF CONTENTS Warranty Information…………………………………………. Section 1. Omni Tip™ Tissue Homogenizing Kit 1.1 Omni Tip Kits………………………………………………….. 1.2 Optional Accessories…………………………………………... Section 2. Omni TH, Tissue Homogenizer 2.1 Specifications…………………………………………………... 2.2 Parts and Accessories…………………………………………. 2.3 Stand-Mounting the Omni TH…………………………...…….. Section 3. Omni TH Operation 3.1 Installing Rotor-Stator Generator Probes……………………...
  • Page 5: Section 1. Omni Tip™ Tissue Homogenizing Kit

    SECTION 1. OMNI TIP™ TISSUE HOMOGENIZING KITS 1.1 OMNI TIP™ KITS Omni’s convenient Tissue Homogenizing Kits come with everything needed to homogenize tissue samples quickly and efficiently for successful RNA extraction. Each kit includes an Omni TH Homogenizer with an adapter for mounting Omni Tips™...
  • Page 6: Optional Accessories

    SECTION 1. OMNI TIP™ TISSUE HOMOGENIZING KITS 1.2 OPTIONAL ACCESSORIES Omni Tip™ Homogenizing Kits are supplied with a handheld Omni TH Homogenizer. For applications where hands-free operation is desired, an optional stand assembly is available. A post clamp assembly is included with the Omni TH for post-mounting the unit to any comparable size ring stand.
  • Page 7: Section 2. Omni Th, Tissue Homogenizer

    Prior to operation, please remove all parts from the shipping container and inspect for damaged or missing parts. If any parts are found to be damaged or missing, please contact Omni International at 1-800-776-4431. The Omni TH, Tissue Homogenizer consists of the following:...
  • Page 8: Stand-Mounting The Omni Th

    SECTION 2. OMNI TH, TISSUE HOMOGENIZER (cont.) 2.3 STAND-MOUNTING THE OMNI TH (Optional) To mount the Omni TH, Tissue Homogenizer to a stand assembly, refer to the figure and follow these steps: 1) Secure the 24” post by twisting it into the base plate until securely tightened.
  • Page 9: Omni Tip™ Plastic Probes

    SECTION 3. OMNI TH OPERATION 3.2 OMNI TIP™ PLASTIC PROBES An Omni Tip™ Adapter (A1000SB) is required to use Omni Tip™ Plastic Probes with the Omni TH Homogenizer. First, insert the smaller end of the Omni Tip Adapter into the Omni TH as far as possible, turn clockwise, and release.
  • Page 10: Theory Of Operation

    SECTION 3. OMNI TH OPERATION NOTE: For optimal sample recovery during processing, reduce the motor speed and completely remove the generator probe from the sample. Then turn off the motor drive unit. WARNING: DO NOT process pathogenic material in an open container, since aerosols created during normal processing could be inhaled by the operator.
  • Page 11: Section 4. Operational Guidelines

    SECTION 4. OPERATIONAL GUIDELINES 4.1 OPERATIONAL GUIDELINES Rotor-stator homogenization provides highly efficient cellular lysis and membrane disruption while reducing the high-molecular weight of cellular components. Omni Tip Tissue Homogenizing Kits allow rapid and complete disruption of cell lysates to expose intracellular contents and ensure increased yields.
  • Page 12 Round vessels encourage swirling. Fluted or cornered vessels disrupt flow patterns, allowing for more effective mixing/processing. Should further information or assistance be required for using Omni Tip™ Homogenizing Kits, please contact Omni International, Inc. Toll free: 1-800-776-4431 Outside the US: 1-770-421-0058...
  • Page 13: Section 5. Omni Tip™ Plastic Probes

    SECTION 5. OMNI TIP™ PLASTIC PROBES 5.1 SPECIFICATIONS 7mm Diameter Disposable Plastic Generator Probe Minimum Vessel Inside Diameter: 8mm Applications: Soft Tissue Omni Tips: liquid/liquid or soft tissue processing Hard Tissue Omni Tips: frozen or fibrous tissue processing Processing Range: 0.25–30ml Length: 110mm The Omni Tips™...
  • Page 14: Installing Omni Tips

    SECTION 5. OMNI TIP™ PLASTIC PROBES WARNING: Safety glasses are recommended when using Omni Tip™ plastic probes. It is possible for plastics to shatter or break after exposure to certain chemicals, or after repeated autoclaving. CAUTION: Large, solid particles, such as frozen tissue, may cause damage to the standard Omni Tip™...
  • Page 15: Autoclaving Omni Tips

    SECTION 5. OMNI TIP™ PLASTIC PROBES 5.4 AUTOCLAVING OMNI TIPS™ Omni Tips™ generator probes can be autoclaved up to seven times and re- tain their mechanical properties. Due to different combinations of chemicals and environments, it is suggested that the autoclaving recommendations be followed as a guideline only, and the user should decide how to best alter the procedure for their specific application.
  • Page 16: Section 6. Motor Maintenance

    It probably requires a new set of brushes. The average brush life for the Omni TH, Tissue Homogenizer is 80-100 hours. For service, or brush replacement, please contact Omni International at 1-800-776-4431. 6.3 MOTOR BEARINGS The Omni TH, Tissue Homogenizer motor is equipped with sealed ball bearings.
  • Page 17: Wiring Diagram

    SECTION 6. MOTOR MAINTENANCE WARNING: DO NOT modify the plug or cord that is provided. If the plug will not fit the outlet, have the proper outlet installed by a qualified electrician. WARNING: Reduce the risk of unintentional starting; make sure the power switch is in the OFF position before plugging in the motor.
  • Page 18: Replacement Parts

    SECTION 6. MOTOR MAINTENANCE 6.6 REPLACEMENT PARTS Table of Replacement Parts for the Omni TH, Tissue Homogenizer # Description 115V 220V 1 Aluminum Washer 12-113 12-113 2 Wave Spring 00-544 00-544 3 Set Screws 00-695 00-695 4 Coupling 12-112 12-112 5 Bearing Insulator 12-114 12-114...
  • Page 19: Section 7. Service

    SECTION 7. SERVICE 7.1 A SSISTANCE Should this product ever require service, please contact Omni International at 1–800–776-4431. 7.2 D ECONTAMINATION Should an instrument or component that has been used with radioactive or pathogenic material require factory or field service, comply with the following procedure to ensure the safety of service personnel: ...
  • Page 20 935-C Cobb Place Blvd. Toll Free: 1-800-776-4431 Kennesaw, GA 30144 Phone: 770-421-0058 Email: omni@omni-inc.com FAX: 770-421-0206 Patents Pending Rev. 01/25/11 rb P/N: PCR-258 www.omni-inc.com...

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