Kurabo QuickGene-Auto240L Operation Manual
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Kurabo QuickGene-Auto240L Operation Manual

Automated nucleic acid isolation system
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Operation Manual
Automated Nucleic Acid Isolation System
QuickGene-Auto240L
Ver. 1.0.1

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Summary of Contents for Kurabo QuickGene-Auto240L

  • Page 1 Operation Manual Automated Nucleic Acid Isolation System QuickGene-Auto240L Ver. 1.0.1...
  • Page 3 Introduction Thank you very much for purchasing our Automated Nucleic Acid Isolation System QuickGene-Auto240L (hereafter referred to as "this system"). This document contains important information about correct and safe use of the functions of this system. Before using, be sure to read this document. For quick reference, please keep this document within easy reach of this system.
  • Page 4 "Biohazard" indicates a risk of infection. Caution: "Caution" indicates a risk of lighter or moderate injury, or property damage. Important: "Important" indicates critical or prohibited actions. Note: "Note" indicates precautions, instructions to abide by, supplemental explanations, etc. QuickGene-Auto240L Operation Manual...
  • Page 5 ・ Use genuine parts designated by our company. ・ The repair of this system shall be conducted by a contractor designated by our company. ・ Use reagents and consumables included in our genuine parts and reagent kits. QuickGene-Auto240L Operation Manual...
  • Page 6 (a permanent outlet, etc.) QuickGene-Auto240L Kit (Hereafter, the QuickGene-Auto240L Kit is referred to as a "dedicated kit" in this document.) ・ Refer to the procedures for use of the dedicated kit in the handbook attached to the kit.
  • Page 7 Warning: of ultraviolet leakage from the sliding door window, fan area, joints with exteriors, etc. Exposure to ultraviolet light may cause damage such as eye or skin irritation. Avoid exposure of skin or eyes to ultraviolet light. QuickGene-Auto240L Operation Manual...
  • Page 8 Warning Labels Warning, caution, instruction and obligation labels are affixed to this system. QuickGene-Auto240L Operation Manual...
  • Page 9 Do not work for a long time in the proximity during UV irradiation. Risk of damage to eyes and skin. Caution: Damage Do not apply force to the flap door by leaning or sitting on it or by placing any objects on it. Doing so may cause damage or injury. QuickGene-Auto240L Operation Manual...
  • Page 10 Restrictions on Use This section indicates the restrictions on use of QuickGene-Auto240L and QuickGene Consumables. ・ No liability is assumed for the results regardless of the purpose of use. ・ Validation of performance on samples shall be based on the judgment and responsibility of the user.

  • Page 11: Table Of Contents

    Preparation of Consumables and Accessories Preparation of Samples Start-up of System Registration and Deletion of User IDs Isolating Operation Operation to Stop Automatic Operation 4 Operating Procedures (Semi-Automatic Protocol) p. 65 For Isolating Operation Confirmation of Articles to be Prepared QuickGene-Auto240L Operation Manual...
  • Page 12 Cleaning of Wash Buffer Feeding Line UV (Ultraviolet Light) Irradiating Function 8 Before Concluding as a Failure p. 127 Troubleshooting Error Messages Appendix A p. 141 Options Warranty After-Sales Services Customer Consultation Desk Precautions for Transportation Disposal QuickGene-Auto240L Operation Manual...
  • Page 13 The procedures for daily inspection and maintenance are explained. 8 Before Concluding as a Failure The handling procedures when you consider a failure has occurred are explained. Appendix A The warranties, after-sales services and precautions for transportation are explained. QuickGene-Auto240L Operation Manual...
  • Page 14 QuickGene-Auto240L Operation Manual...

  • Page 15: System Installation Procedures

    Required footprint: Width ≧178 cm × Depth ≧152 cm × Height ≧205 cm (when placed on an 80-cm high experiment table) (20 cm) 205 cm 10cm (10 cm) (10 cm) (40 cm) (70 cm) 152 cm 178 cm QuickGene-Auto240L Operation Manual...
  • Page 16 10-80 During standby Max. Wet-bulb Temp. In operation 29 (no condensation) During standby 29 (no condensation) ≦12 (no condensation) Temperature Gradient C / Hr) Humidity Gradient ≦30 (no condensation) (% / day) Use at ≦1600 Altitude (m) QuickGene-Auto240L Operation Manual...

  • Page 17: How To Open The Package

    1 System Installation Procedures 1.2 How to Open the Package QuickGene-Auto240L unpacking instruction Refer to the separately attached " " QuickGene-Auto240L Operation Manual...

  • Page 18: How To Remove The Interior Packing Material And Fixtures

    1 System Installation Procedures 1.3 How to Remove the Interior Packing Material and Fixtures QuickGene-Auto240L Fixture Removing Procedures Refer to the separately attached " ". QuickGene-Auto240L Operation Manual...

  • Page 19: Checking Of Packed Contents

    □ Waste Tube Holder ×3 □ Collection Tube Holder ×3 □ Waste Container □ Wash Buffer Bottle □ Reagent Container S ×3 □ Reagent Container L ×4 □ UV Lamp Caution: Note: Use power cables, holders, containers included in package QuickGene-Auto240L Operation Manual...

  • Page 20: Names Of Parts

    Flap Door (L) Power Switch Power Cable Flap Door (R) Drawer ■ System Side View Right Side Left Side Power Cable Not Connected Power Cable Connected Inlet Power Cable Port 2 Port Serial Port Port 1 (9 pin) QuickGene-Auto240L Operation Manual...
  • Page 21 Lysate Unit Collection Tube Holder Sample Tip Holder Slot Slot Reagent Tip Holder Slot Cartridge/Waste Tube Holder Slot Sample Holder Slot ■ Inside Drawer Wash Buffer Feed Pump Breaker Switch Waste Container Rack Wash Buffer Bottle Rack QuickGene-Auto240L Operation Manual...
  • Page 22 1 System Installation Procedures ■ Robot Unit Laser Sensor (Rear) [For detection of Work/Consumables] Wash Buffer Nozzle Pressurizing Nozzle/ Pressurizing Packing Dispenser Drip Tray Level Detection Monitor Panel Tip Pressure Monitor Panel Syringe UV Lamp QuickGene-Auto240L Operation Manual...

  • Page 23: Mounting Of Uv Lamp

    (1) Insert the UV lamp along the vertical groove of the lamp socket (to the end). (2) Turn the UV lamp 90° to mount on the lamp socket. (Turn until a clicking sound is heard.) NB: To remove the UV lamp, follow the reverse order of above. QuickGene-Auto240L Operation Manual...

  • Page 24: Checking The System Functions

    ・ Clean the fluid feeding line with wash buffer before storage.* ・ If the system will be stored for a lengthy period, place an appropriate cover over it for protection from dust and staining. * Refer to "7 Daily Inspections and Maintenance" QuickGene-Auto240L Operation Manual...

  • Page 25: Basic Functions

    2 Basic Functions The QuickGene-Auto240L is a system that can isolate high-purity and high-yield DNA in a short time. All of the processes from the primary tube (from 2 mL of whole blood) to the collection of DNA are automatically processed within the system.
  • Page 26 The wash buffer used for isolation is fed from the feeder zone to Fluid Feeding Zone the wash buffer nozzle of the robot unit in accordance with the isolation program. QuickGene-Auto240L Operation Manual...

  • Page 27: Basic Operations, Basic Functions

    Collection ID Confirmation BACK Confirmation Message Screen (2-Dimensional Cord Reading) * Confirm all the collection racks CANCEL are removed from system Screen Close COMPLETE CANCEL Screen Close A-7-k Confirmation Message BACK BACK A-10 Reagent Confirmation Screen BACK BACK QuickGene-Auto240L Operation Manual...
  • Page 28 EXPERT MODE BARCODE READING C-2 Parameter Setup Screen ELUTION BUFFER VOLUME BACK BACK BACK C-3 Barcode (ID) Reading Function C-5 Expert Mode C-４ Recovering Fluid Quantity Setup Setup Screen Password Input Screen Screen (DISP ELUTION BUFFER (1)) QuickGene-Auto240L Operation Manual...
  • Page 29 CANCEL CANCEL E-8 Wash Buffer Feeding Line E-2-a Confirmation Message E-2-b Confirmation Message Maintenance Screen START START Robot moves to O-ring replacement position Robot moves to pressuring nozzle (packing) maintenance position (Power Switch OFF) (Power Switch OFF) QuickGene-Auto240L Operation Manual...

  • Page 30: Safety Mechanisms

    The system lysate unit is equipped with an open/close type cover to prevent the ejection of the lysate tube during automatic operation. ■ Operation Panel, Alarm The system operating condition is indicated using the operation panel and the alarm. QuickGene-Auto240L Operation Manual...

  • Page 31: Operation Panel Displaying Contents

    [ENT]. Similarly, re-enter an identical number with [PASSWORD] in the [PASSWORD (RE-ENTER)] frame as well and press [ENT]. Finally, press [REGISTER] to register the User ID. If the passwords in the 2 places do not match, the User ID will not be registered. QuickGene-Auto240L Operation Manual...
  • Page 32 (1–24) or the buttons (A, B and C) for sample holder names. Numbers smaller than the pressed one will be automatically selected. By pressing a button with reverse color again, selection of the relevant sample will be canceled. Press [OK] to move on to the next screen. QuickGene-Auto240L Operation Manual...
  • Page 33 The [RESUME] button will be enabled when the operation is suspended. Press [RESUME] to resume automatic operation. QuickGene-Auto240L Operation Manual...
  • Page 34 [PREVIOUS] to go back to the previous item. Press [COMPLETE] while selecting data to save, and then the data can be saved in an external memory after a confirmation message. Press [BACK] to return to the operating history screen. QuickGene-Auto240L Operation Manual...
  • Page 35 ・[ERROR HISTORY]: Error history screen ・[INDIVIDUAL OPERATION]: Individual operation screen (NB: used under instruction of the manufacturer or sales agent) ・[DATE AND TIME SETTING]: Clock setting screen ・[MANUFACTURER MAINTENANCE]: Manufacturer maintenance screen (NB: use by the user is prohibited) QuickGene-Auto240L Operation Manual...
  • Page 36 Touch the white frame for each "MONTH", "DAY", "YEAR", "HH” (hour) and "MM” (minute) to display a ten-key pad, enter a value, and press [ENT]. Finally, press [OK] to set up the clock. QuickGene-Auto240L Operation Manual...
  • Page 37 [ENTER]： Finalize the entered data (Finalization is not accepted if the entered number of characters is insufficient for ID, password, etc.) [BS]： Delete 1 entered digit [CL]： Delete all entered digits [ESC]：Return to previous screen from the ten-key input screen QuickGene-Auto240L Operation Manual...

  • Page 38: Barcode Scanning Functions

    Reads out the collection ID from the Barcode Reader for 2-dimensional code on the bottom surface of a Collection Matrix Tube Option collection tube such as a matrix tube with 2-dimensional code. The ID information readout is checked against the sample ID. QuickGene-Auto240L Operation Manual...

  • Page 39: Basic Specifications

    50-60 Hz Environment for Use Indoor Use Overvoltage Category Transient overvoltage category II Applied Rated Pollution Degree 2 Contamination IP Classification IPX0 Temp. /Humid. Range for Temperature: 15°C-30°C Humidity: 30%-80%RH (no condensation) ≦1600 m for use Altitude QuickGene-Auto240L Operation Manual...

  • Page 40: Product Labeling

    Label indicating the reagent tip holder. Label indicating the sample tip holder. Label indicating reagent container setting position No.1. Label indicating reagent container setting position No.2. Label indicating reagent container setting position No.3. Label indicating reagent container setting position No.5. QuickGene-Auto240L Operation Manual...
  • Page 41 The numbers 1-6 indicate the setting positions for reagent containers in the holder, and “WASTE” indicates the setting position for the waste fluid container. Label indicating setting position for waste container. The setting position is in a location pointed with an arrow. Label indicating reagent container setting position No.4. QuickGene-Auto240L Operation Manual...
  • Page 42 Affixed on the bottom right side surface of this system (front side). “MODEL” indicates the model number of this system. “INPUT” indicates the input power voltage, current and frequency. “S/N” indicates the serial number. CE symbol marks are clearly stated. QuickGene-Auto240L Operation Manual...

  • Page 43: Implemented Protocols

    The parameter “W BLOOD DNA 2.0 mL FULL-AUTO” is (Whole CUSTOM 1 (Full-Auto) (DNA) input in protocols 1-4 as a default Blood 2 mL) value. To change the parameters, a separate input of EXPERT PASSWORD is required. QuickGene-Auto240L Operation Manual...
  • Page 44 2 Basic Functions QuickGene-Auto240L Operation Manual...

  • Page 45: How To Operate (Full-Automatic Protocol)

    In addition, pay attention to the risk of causing contamination or system failure. Waste Tube Collection Tube Cartridge Rule 1. Holder Holder Holder Waste tubes, cartridges and collection tubes should be set in order from opposite side of handle. Handle QuickGene-Auto240L Operation Manual...
  • Page 46 A→B→C. Use holder B (or C) when waste tubes, cartridges and collection tubes are occupied in all positions in holder A (or A and B) All positions are occupied → Use the next holder Handle QuickGene-Auto240L Operation Manual...

  • Page 47: Confirmation Of Articles To Be Prepared

    QuickGene DNA Whole Blood kit L (DB-L) for 48 Specimens/1 Kit - Cartridge x 48 - Waste Tube x 48 - Reagents x 1 set ◆ QuickGene-Auto240L Consumables Kit (QG-240L-CK) for 48 Specimens/1 Kit - Lysate Tube x 48 - 10-mL Tip x 60 - 1.2-mL Tip x 96 ◆...

  • Page 48: Preparation Of Reagents

    Add 160 mL of special grade ethanol in the bottle before use and mix well. After mixing with ethanol, close the bottle lid and preserve at room temperature. ◆ Elution Buffer (CDB) Used for elution of nucleic acid QuickGene-Auto240L Operation Manual...
  • Page 49 Set an empty container at the setting position numbers for reagent containers not to be used and waste containers. Setting Position 6 (Empty) For Additional Reagent Waste Container 1 (Empty) Waste Container 2 (Empty) QuickGene-Auto240L Operation Manual...
  • Page 50 The wash buffer bottle is set in such a way that the opening comes to the left side of the rack. ・ The ends of 2 inlet tubes must reach the bottom of the wash buffer bottle. ・ The inlet tubes should not be kinked midway. QuickGene-Auto240L Operation Manual...

  • Page 51: Preparation Of Consumables And Accessories

    12 Note: Set all reagent tips (1.2ml tips x 12, 10ml tips x4) in the holder Set the number of tips for lysate (10ml x 1 – 24) equal or more to the number of samples QuickGene-Auto240L Operation Manual...
  • Page 52 Use the adapters according to the type of collection tubes. Adapter order Setting 1.5ml Micro Tube Matrix Tube with 2D (without adapter) Barcode Handle (with adapter) Note: When several holders are used, set the collection tubes while paying attention to the holder identification symbols A-C. QuickGene-Auto240L Operation Manual...
  • Page 53 Set a holder in the correct slot according to the manual and the identification label. If a holder is forcibly set in a wrong slot, the holder or system may be damaged. (3) Close the left/right flap doors. QuickGene-Auto240L Operation Manual...
  • Page 54 ○ Note: Be sure to securely close and fix the agitator cover until a click is heard. If the fixing is insecure, an error will occur during the system check before operation. (5) Close the sliding doors QuickGene-Auto240L Operation Manual...
  • Page 55 Be sure to correctly set an empty waste container included in the delivery. The use of container not included in the delivery, use of un-emptied container or use with an erroneous setting may cause waste overflow. (3) Close the drawer QuickGene-Auto240L Operation Manual...

  • Page 56: Preparation Of Samples

    Setting Order for Blood Collection Tubes セット順序 Barcode Reading Surface Handle Adaptor Note： When several holders are used, set the blood collection tubes while paying attention to the holder identification symbols A – C. QuickGene-Auto240L Operation Manual...
  • Page 57 The holder should be securely set in the slot until it contacts the stopper on the end of the system. ・ Set a holder in the correct slot according to the manual and the identification label. If a holder is forcibly set in a wrong slot, the holder or system may be damaged. QuickGene-Auto240L Operation Manual...

  • Page 58: Start-Up Of System

    (3) Confirm that the sliding doors, left/right flap doors and drawer are closed. The power can be turned ON with doors open, but power will not be supplied to the driving system and the system check will not be conducted. QuickGene-Auto240L Operation Manual...
  • Page 59 (3) Press [OK] when the system check for all the items is complete and move on to the user sign on screen. If an item with an indication of [NG] is found, press [NG], confirm the error message, and refer to "8.2 Error Messages" for solving a problem. QuickGene-Auto240L Operation Manual...
  • Page 60 Refer to "3.8 Isolating Operation". OPERATING HISTORY / Operation History Mode Refer to "5 Operation History". PARAMETER SETUP / Parameter Setup Mode Refer to "6 Parameter Set-up Procedures". MAINTENANCE / Maintenance Mode Refer to "7 Daily Inspection and Maintenance". QuickGene-Auto240L Operation Manual...

  • Page 61: Registration And Deletion Of User Ids

    5-10 digit number, and press [ENT] Set up a password for the user ID. (4) Re-enter an identical number with [PASSWORD] in the [PASSWORD (RE-ENTER)] frame and press [ENT]. (5) Press [REGISTER] and register the User ID. QuickGene-Auto240L Operation Manual...
  • Page 62 (3) Touch the white frame of [EXPERT PASSWORD] to display a ten-key pad, enter a password for an expert, and press [ENT]. Contact our sales agent regarding the password for an expert. (4) Press [DELETE] to delete the User ID QuickGene-Auto240L Operation Manual...

  • Page 63: Isolating Operation

    (4) Press the full automatic protocol button to operate. (Ex：W BLOOD DNA 2 mL FULL-AUTO) Refer to "2.8 Implemented Protocols" for explanation on protocols. (5) Move on to the next section "<2> Enter Sample Information". QuickGene-Auto240L Operation Manual...
  • Page 64 Check for the correctness of readout positional information and the position of the set sample. (A-4) If there are more holders to set, press [NEXT] and carry out (A-2), (A-3) for holders B, C. QuickGene-Auto240L Operation Manual...
  • Page 65 Pressing A, B or C button will enable selection of all for each holder row. (B-2) Check that the selected button coincides with the number of set samples and press [OK]. (B-3) Move on to the next section "<3> Enter Collection Information" QuickGene-Auto240L Operation Manual...
  • Page 66 ID and directly view the red light source. Direct viewing at length may cause eye 2D Barcode Reader disorders. Note: If a pop-up window is not displayed, check the set position of collection tubes and execute the reading again. QuickGene-Auto240L Operation Manual...
  • Page 67 D. When the collection ID reading function is OFF When the collection ID reading function is OFF, refer to “3.4 Consumables and Accessories” Note: and set the collection tube holders before turning system power ON. (D-1) Go to the next section “<4> Confirmation of Reagent” QuickGene-Auto240L Operation Manual...
  • Page 68 (1) Press [CHECK] for a confirmed reagent. If [ALL] is pressed, all [CHECK] buttons are pressed at the same time. (2) Press [OK]. When all [CHECK] buttons are pressed, the [OK] button will be enabled. QuickGene-Auto240L Operation Manual...
  • Page 69 90 74 58 42 26 10 91 75 59 43 27 11 92 76 60 44 28 12 93 77 61 45 29 13 94 78 62 46 30 14 95 79 63 47 31 15 96 80 64 48 32 16 QuickGene-Auto240L Operation Manual...
  • Page 70 If the set sample quantity is more than 3 mL, press [BACK] to go back to the mode select screen, press [UNLOCK] to release the door locks, and then take out the sample holder from the system and adjust the sample quantity. QuickGene-Auto240L Operation Manual...
  • Page 71 DISPENSING TIP (1.2ml/REAGENT) Consumables/ DISPENSING TIP (10ml/REAGENT) Accessories CARTRIDGE/WASTE TUBE WASTE CONTAINER AGITATOR COVER Note: When the isolating operation is suspended due to an error or trouble during automatic operation, refer to “8.1 Troubleshooting” or “8.2 Error Messages” QuickGene-Auto240L Operation Manual...
  • Page 72 When the collection ID reading function is [OFF], the sample position information (Ex: A-1, A-2) is displayed in the collection ID columns. The ID information will be confirmed and saved later. Refer to “5 Operation History” for the procedures. QuickGene-Auto240L Operation Manual...
  • Page 73 3 Operation Procedures - Full Auto Protocols Press [FINISH] on the operation result screen. Press [OK] in the displayed pop-up window. (5) Press the system power switch and turn the power OFF. QuickGene-Auto240L Operation Manual...
  • Page 74 (2) Close the collection tube cap (if a cap is used) and remove the tube Note: ・ Securely close the cap. ・ If the collection ID reading function is “OFF”, carefully conduct the removal and management of collection tubes paying attention to the holder identification symbols A-C. QuickGene-Auto240L Operation Manual...
  • Page 75 Lysate Tube Dispose of lysate tubes Biohazard: Dispose of lysate tubes in accordance with customer's infectious waste treatment manuals. QuickGene-Auto240L Operation Manual...
  • Page 76 If the operation is to be continued with a full-automatic protocol, start from "3.3 Preparation of Reagent". Important: If the system will not be used again for more than 1 week, refer to "7.2 When System Not in Use for More than One Week" and carry out maintenance. QuickGene-Auto240L Operation Manual...
  • Page 77 Once power is shut down, no resumption of the stopped movement is possible. ・ Even if operation is resumed after stoppage, a sufficient quality/yield of nucleic acid may not be achievable if the stoppage was repeated or lengthy in duration. QuickGene-Auto240L Operation Manual...
  • Page 78 3 Operation Method - Full-Auto Protocol QuickGene-Auto240L Operation Manual...

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