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multiSUB® Electrophoresis Systems
MSMINI MSMIDI MSCHOICE MSCHOICEST MSMAXI
Instruction Manual
Catalogue No:
Version 1
MSMINI7, MSMINI10, MSMINIDUO
MSMIDI7, MSMIDI10, MSMIDIDUO
MSCHOICE7, MSCHOICE10, MSCHOICE15, MSCHOICETRIO
MSCHOICEST20, MSCHOICEST25
MSMAXI10, MSMAXI15, MSMAXI20, MSMAXIDUO, MSMAXI25
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Summary of Contents for Cleaver Scientific multiSUB MSMINI
- Page 1 multiSUB® Electrophoresis Systems MSMINI MSMIDI MSCHOICE MSCHOICEST MSMAXI Instruction Manual Catalogue No: MSMINI7, MSMINI10, MSMINIDUO MSMIDI7, MSMIDI10, MSMIDIDUO MSCHOICE7, MSCHOICE10, MSCHOICE15, MSCHOICETRIO MSCHOICEST20, MSCHOICEST25 MSMAXI10, MSMAXI15, MSMAXI20, MSMAXIDUO, MSMAXI25 Version 1...
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Page 2: Table Of Contents
Table of Contents Page Section 1 Safety Information Safety Precaution Section 2 General Information Packing Lists Care and Maintenance Section 3 Operating Instructions Usage Guidance and restrictions Setting up the Horizontal Gel Tanks Gel Preparation Gel Pouring Running the Gel Gel Staining and Viewing Solutions Section 4... -
Page 3: Safety Information
Section 1 Safety Information 1.1 Safety Precaution When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. -
Page 4: General Information
Section 2 General Information 2.1 Packing Lists MSMINI7, MSMINI10, MSMINIDUO Units include tank, lid and electrodes and include the following accessories: Tray Tray Dams Combs Loading Guides Cables 2 x MS7-8-1 MS7-LG – Strips MS7-UV7 MS7-UVDAM CSL-CAB MSMINI7 1mm, 8 MS7-WP –... - Page 5 MSCHOICE7, MSCHOICE10, MSCHOICE15, MSCHOICETRIO Units include tank, lid and electrodes and include the following accessories: Tray Tray Dams Combs Loading Guides Cables 2 x MS15-20- MS15-LG – MS15-UV7 MS15- MSCHOICE7 Strips 15 x 7cm UVDAM CSL-CAB 1mm, 20 MS15-WP – (W x L) Pack of 2 sample...
- Page 6 MSMAXI10, MSMAXI15, MSMAXI20, MSMAXIDUO, MSMAXI25 Units include tank, lid and electrodes and include the following accessories: Tray Tray Combs Loading Guides Cables Dams 2 x MS20-20- MS20-UV10 MS20- MS20-LG – Strips MSMAXI10 20 x 10cm (W x UVDAM MS20-WP – CSL-CAB 1mm, 20 Pack of 2...
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Page 7: Care And Maintenance
2.2 Care and Maintenance Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60 C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent buildup of salts but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised. -
Page 8: Operating Instructions
Section 3 Operating Instructions Further information (including videos) regarding setting up and running the multiSUB® units can be found at www.cleaverscientific.com 3.1 Usage Guidance and restrictions • Maximum altitude 2,000m. • Temperature range between 4°C and 65°C. • Maximum relative humidity 80% for temperatures up to 31 C decreasing linearly to 50% relative humidity at •... -
Page 9: Setting Up The Horizontal Gel Tanks
3.2 Setting up the Horizontal Gel Tanks Instructions for fitting Electrode Cables 1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of the cables, black is negative and red positive. 2. -
Page 10: Gel Preparation
3.3 Gel Preparation 1. Table 1 below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size. For a standard 0.7% agarose gel, add 0.7 grams of agarose to 100 ml of 1x TAE or TBE solution. The same 1 x solution should be used in the tank buffer solution. -
Page 11: Gel Pouring
3.4 Gel Pouring The CSL Multi sub range of units allows three different methods of gel casting: Casting Dams Flexicaster Traditional Tape Using trays with Casting Dams 1. To fit the casting dams, place one casting dam on the bench with the groove facing upwards (see figure 1). Push the edge of the tray down firmly into the groove (see figure 2). - Page 12 Using The Flexicaster See figures 4 and 5 for Flexicaster MS7-FC and MS20-FC 1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly central. 2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster.
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Page 13: Running The Gel
must be taken to ensure that samples from the first wells do not migrate into the lanes of the second comb wells. 3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip. -
Page 14: Gel Staining And Viewing
3.6 Gel Staining and Viewing The Multi Sub trays allow staining to be performed without removing the gel from the tray if this is preferred. 1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium bromide stain for 15– 30 minutes, see solutions for stock stain concentration and adjust to the volume used accordingly. -
Page 15: References
1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA 10x (1L) dissolve in 750 ml distilled water: 108 g tris base (FW = 121) 55 g boric acid (FW = 61.8) 40 ml 0.5 M EDTA (pH 8.0) Fill to 1 litre with distilled water. -
Page 16: Trouble Shooting Guide
Review enzyme activity, digest further. Band smearing and streaking Agarose has improper endosmosis Consult Cleaver Scientific about agarose. Salt concentration in sample too high Reduce salt concentration to ≤0.1M. Excessive power and heating Reduce voltage. See electrophoresis instructions. Sample spilled out of well Apply sample carefully. -
Page 17: Product Information
Section 6 Product Information 6.1 Catalogue numbers and product descriptions for multiSUB® units & accessories Catalogue No. Product description MSMINI7 multiSUB® Mini, 7 x 7cm UV Tray, 2 x 8 sample combs, loading guides and dams MSMINI10 multiSUB® Mini, 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams MSMINIDUO multiSUB®... - Page 18 MSCHOICE7 multiSUB® ™ Choice 15 x 7cm UV Tray, 2 x 20 sample combs, loading guides and dams MSCHOICE10 multiSUB® ™ Choice 15 x 10cm UV Tray, 2 x 20 sample combs, loading guides and dams MSCHOICE15 multiSUB® ™ Choice 15 x 15cm UV Tray, 2 x 20 sample combs, loading guides and dams MSCHOICETRIO multiSUB®...
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Page 19: Combs
6.2 Combs – MC Denotes Multi Channel Pipette compatible multiSUB® Mini multiSUB® Midi multiSUB® Choice Multi Sub Maxi 0.75mm 0.75mm 0.75mm 0.75mm No. of Part Number No. of Part Number No. of Part Number No. of Part Number Samples Samples Samples Samples MS20-1-0.75... - Page 20 multiSUB® Mini multiSUB® Midi multiSUB® Choice Multi Sub Maxi 1.5mm 1.5mm 1.5mm 1.5mm No. of Part Number No. of Part Number No. of Part Number No. of Part Number Samples Samples Samples Samples MS7-1-1.5 MS10-1-1.5 MS15-1-1.5 MS20-1-1.5 MS7-2-1.5 MS10-2-1.5 MS15-2-1.5 MS20-2-1.5 MS7-4-1.5 MS10-4-1.5...
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Page 21: Catalogue Numbers And Product Descriptions For Csl Related Products
6.3 Catalogue numbers and product descriptions for CSL Related Products Catalogue No. Product description nanoPAC-300 Mini Power supply, 300V, 400mA, 60W -100 -240VAC nanoPAC-500 Mini Power supply, 500V, 400mA, 120W -100 -240VAC CS300V omniPAC MIDI Power Supply, 300V, 700mA, 150 – 100 -240VAC OmniDOCIPROSAFE OMNIDOC-i plus Blue LED Epi-illumination Module (OMNIDOC-BL), and 520, 560 &... -
Page 22: Warranty
Section 7 Warranty The Cleaver Scientific Ltd. (CSL) multiSUB® Horizontal Electrophoresis units have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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