Cleaver Scientific multiSUB MSMINI7 Instruction Manual
Horizontal electrophoresis units
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Summary of Contents for Cleaver Scientific multiSUB MSMINI7
- Page 1 multiSUB Horizontal System...
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Page 3: Instruction Manual
multiSUB Horizontal ® Electrophoresis Units Instruction Manual Catalogue Numbers MSMINI7 MSMIDI7 MSMINI10 MSMIDI10 MSMINIDUO MSMIDIDUO MSCHOICE7 MSMAXI10 MSCHOICE10 MSMAXI15 MSCHOICE15 MSMAXI20 MSCHOICETRIO MSMAXIDUO MSCHOICEST20 MSMAXI25 MSCHOICEST25 Record the following for your records: Model _____________________ Catalogue No. _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No. -
Page 4: Table Of Contents
Contents Instruction Manual Catalogue Numbers Contents Safety Information Packing List Specifications Operating Instructions Usage Guidance and restrictions Setting up the Horizontal Gel Tanks Fitting Electrodes Fitting Loading Guides Gel Preparation Gel Pouring Casting Dams Flexicaster Tape Running the Gel Gel Staining and Viewing Solutions References Troubleshooting... - Page 5 15/11/2018 Page 3...
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Page 6: Safety Information
Safety Information When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly. -
Page 7: Packing List
Packing List Each multiSUB unit includes a tank, wired electrodes, lid and the following items: Tray Tray Dams Combs Loading Guides Cables MSMINI7 MS7-UV7, 7 x MS7-UVDAM, 2 x MS7-8-1, MS7-LG – Strips, CSL- 7cm (W x L) Pack of 2 1mm, 8 sample MS7-WP –... - Page 8 The unit should be checked for damage when received. Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue.
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Page 9: Specifications
Specifications Power supply cables 1500V rated with retractable 4mm connectors Safety lid and viewing pane High impact moulded acrylic construction Height adjustable combs Acrylic UV transparent gel tray UV transparent above 300nm, Moulded acrylic Comb Slots Allow multiple combs per tray Casting dams Natural rubber Colour-coded electrodes... -
Page 10: Operating Instructions
Operating Instructions Further information (including videos) regarding setting up and running the multiSUB units can be found at www.cleaverscientific.com ® Usage Guidance and restrictions Maximum altitude 2,000m. Temperature range between 4°C and 65°C. Maximum relative humidity 80% for temperatures up to 31 decreasing linearly to 50% relative humidity at 40 ... -
Page 11: Gel Preparation
2. Remove the tray. 3. Peel the back off the loading guide and carefully apply the loading guide directly to the gel platform. The unit is now ready to be used. Gel Preparation The Table below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size. -
Page 12: Gel Pouring
should be heated until all crystals are dissolved. This is best viewed against a light background. Crystals appear as translucent crystals. These will interfere with sample migration if not completely dissolved. The gel must be cooled to between 50°C and 60°C degrees before pouring. Gel Pouring The multiSUB range of units allows three different methods of gel casting:... - Page 13 5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank. 15/11/2018 Page 11...
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Page 14: Flexicaster
Flexicaster 1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly central. 2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster. -
Page 15: Tape
Tape 1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray and stuck m1cm in from the tray edge. -
Page 16: Gel Staining And Viewing
Gel Staining and Viewing The Multi Sub trays allow staining to be performed without removing the gel from the tray if this is preferred. 1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium bromide stain for 15–30 minutes, see solutions for stock stain concentration and adjust to the volume used accordingly. -
Page 17: References
10X TBE stock (1L) dissolve in 750 ml distilled water: 108 g tris base (FW = 121) 55 g boric acid (FW = 61.8) 40 ml 0.5 M EDTA (pH 8.0) Fill to 1 litre final volume with distilled water Loading Dye 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE buffer. -
Page 18: Troubleshooting
Troubleshooting Problem Cause Solution Gel agarose percentage too Decrease agarose percentage. Bands sharp but not enough high Review enzyme activity, digest bands seen Incomplete digestion further. Reduce salt concentration to Agarose has improper ≤0.1M. endosmosis Reduce voltage. See Salt concentration in sample too electrophoresis instructions. - Page 19 15/11/2018 Page 17...
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Page 20: Care And Maintenance
Care and Maintenance Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60°C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts, but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised. -
Page 21: Ordering Information
Ordering information Catalogue Product description MSMINI7 multiSUB® Mini, 7 x 7cm UV Tray, 2 x 8 sample combs, loading guides and dams MSMINI10 multiSUB® Mini, 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams MSMINIDUO multiSUB®... - Page 22 MS10-UVS 10cm UV Gel Scoop CSL-CAB Electrophoresis cable (Black & Red) MSCHOICE7 multiSUB® ™ Choice 15 x 7cm UV Tray, 2 x 20 sample combs, loading guides and dams MSCHOICE10 multiSUB® ™ Choice 15 x 10cm UV Tray, 2 x 20 sample combs, loading guides and dams MSCHOICE15 multiSUB®...
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Page 23: Comb Options
MS20-UV15 20 x 15cm UV Tray MS20-UV20 20 x 20cm UV Tray MS20-UV25 20 x 25cm UV Tray MS20-UVDAM Casting Dams MS15/20-FC multiSUB Choice/Maxi Flexi caster MS20-LG Adhesive Loading Guides MS20-WP Viewing Platform MS20-PE Positive Electrode MS20-NE Negative Electrode MS20-UVS 20cm UV Gel Scoop MSMAXICP Cool-Pack and Platform... - Page 24 MS7-4-1.5 Mini 10.63 MS7-8MC-1.5 Mini MS7-8-1.5 Mini MS7-10-1.5 Mini MS7-12-1.5 Mini MS7-16-1.5 Mini MS7-1-2 Mini MS7-2-2 Mini MS7-4-2 Mini 10.63 MS7-8MC-2 Mini MS7-8-2 Mini MS7-10-2 Mini MS7-12-2 Mini MS7-16-2 Mini MS10-1-0.75 Midi 0.75 MS10-2-0.75 Midi 0.75 MS10-4-0.75 Midi 0.75 MS10-8-0.75 Midi 0.75 MS10MC-10-0.75 Midi...
- Page 25 MS10MC-10-1.5 Midi MS10-12-1.5 Midi MS10-16-1.5 Midi MS10-20MC-1.5 Midi MS10-1-2 Midi MS10-2-2 Midi MS10-4-2 Midi MS10-8-2 Midi MS10MC-10-2 Midi MS10-12-2 Midi MS10-16-2 Midi MS10-20MC-2 Midi MS15-1-0.75 Choice 0.75 MS15-2-0.75 Choice 0.75 MS15-4-0.75 Choice 0.75 MS15-10-0.75 Choice 0.75 MS15-10MC-0.75 Choice 0.75 MS15-12-0.75 Choice 0.75 MS15-14MC-0.75 Choice...
- Page 26 MS15-1-1.5 Choice MS15-2-1.5 Choice MS15-4-1.5 Choice MS15-10-1.5 Choice MS15-10MC-1.5 Choice MS15-12-1.5 Choice MS15-14MC-1.5 Choice MS15-16-1.5 Choice MS15-20-1.5 Choice 4.75 MS15-28MC-1.5 Choice MS15-35-1.5 Choice MS15-1-2 Choice MS15-2-2 Choice MS15-4-2 Choice MS15-10-2 Choice MS15-10MC-2 Choice MS15-12-2 Choice MS15-14MC-2 Choice MS15-16-2 Choice MS15-20-2 Choice 4.75 MS15-28MC-2...
- Page 27 MS20-4-1 Maxi MS20-10-1 Maxi MS20-16-1 Maxi MS20-20MC-1 Maxi MS20-25-1 Maxi MS20-30-1 Maxi MS20-36-1 Maxi MS20-40MC-1 Maxi MS20-1-1,5 Maxi 1013 MS20-2-1.5 Maxi MS20-4-1.5 Maxi MS20-10-1.5 Maxi MS20-16-1.5 Maxi MS20-20MC-1.5 Maxi MS20-25-1.5 Maxi MS20-30-1.5 Maxi MS20-36-1.5 Maxi MS20-40MC-1.5 Maxi MS20-1-2 Maxi 1350 MS20-2-2 Maxi MS20-4-2...
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Page 28: Related Products
Related Products Catalogue No. Product description nanoPAC-300 Mini Power supply, 300V, 400mA, 60W -100 -240VAC nanoPAC-500 Mini Power supply, 500V, 400mA, 120W -100 -240VAC POWERPRO300 MIDI Power Supply, 300V, 700mA, 150 – 100 -240VAC OMNIDOC plus Blue LED Epi-illumination Module (OMNIDOC-BL), and omniDOCPROSAFE 520, 560 &... - Page 29 15/11/2018 Page 27...
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Page 30: Warranty
Warranty The Cleaver Scientific Ltd. (CSL) multiSUB Horizontal Electrophoresis units ® have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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