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Summary of Contents for Cleaver Scientific OmniPAGE Series
- Page 1 OmniPAGE Vertical Electrophoresis Systems OmniPAGE Mini Instruction Manual Catalogue No: CVS10D CVS10DSYS CVS10PRE CVS10DSYS-CU Version 1...
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Page 2: Table Of Contents
Table of Contents Page Section 1 Safety Information Safety Precaution Section 2 General Information Introduction Product Description Packing Lists Specifications Care and Maintenance Section 3 Operating Instructions Setting up the omniPAGE Gel Tank Gel casting omniPAGE module assembly and Sample loading Gel Running Gel Removal Section 4... -
Page 3: Safety Information
Section 1 Safety Information 1.1 Safety Precaution When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. -
Page 4: General Information
Section 2 General Information 2.1 Introduction Cleaver Scientifics’ omniPAGE range of Vertical Gel Units combines ease of use with high resolution separations. Four sizes, Mini 10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm, VS 30 x 30cm share a host of common features including a guaranteed leak proof seal required for trouble free, rapid and uncomplicated gel casting. -
Page 5: Packing Lists
2.3 Packing Lists CVS10D, CVS10DSYS, CVS10PRE, CVS10DSYS-CU Each unit includes a tank, lid, internal module, electrodes and the following accessories: Glass Plates Combs Casting base Cooling Pack Cables Screws CVS10D VS10NG – Notched, 2 of VS10-12-1 VS10ICB CSL-CAB VS10-SCREW x 4 Pk/2 1mm thick, 12 VS10PGS1 –... -
Page 6: Specifications
2.4 Specifications of OmniPAGE Vertical Electrophoresis Units omniPAGE Mini omniPAGE Mini omniPAGE Maxi omniPAGE VS30 Wide Plate 10x10cm 20 X 10cm 20 X 20CM 30 X 22cm Dimensions 7.5x8cm 18 X 8cm 16 X 17.5CM 28 X 20cm Gel Dimensions (WxL) Unit Dimensions 19x13x15cm 26 X 16 X 16cm... -
Page 7: Care And Maintenance
2.5 Care and Maintenance Cleaning Large Format Vertical Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60 C can cause damage to the unit and components. The inner module should be thoroughly rinsed with warm water or distilled water to prevent buildup of salts but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised. -
Page 8: Operating Instructions
Section 3 Operating Instructions 3.1 Setting up the omniPAGE Gel Tank Note: Before setting up the Gel Tank please ensure that it has been properly cleaned and dried. 1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of the cables, black is negative and red positive. - Page 9 Glass cassette Assembly Assemble the glass plates so that the bottom of the glass plates and the spacers are perfectly aligned. For triple plate sandwiches, the free spacers Need to be perfectly aligned which is best performed using a small spacer or comb to push the spacers apart. Notched glass plates with bonded spacers do not need manual alignment.
- Page 10 SCREW VERSION SLIDING CLAMP VERSION Fully tighten the screw for the Mini vertical and the screws sequentially and in an even manner for the maxi vertical in the order middle two, top then bottom, making sure not to wobble the unit. When using the Slide Clamp Mini version, simply slide both gates outwards until fully tightened.
- Page 11 NOTE: It is best to turn the cams in opposite directions to each other. Do not overturn as this will cause the glass plates to push upwards and the assembly will be more likely to leak. The unit is now ready for gel preparation and pouring.
- Page 12 6. Let the resolving gel polymerize. Usually this takes around 15 to 30 minutes but this can vary due to the freshness of the reagents used. If polymerization is taken a lot longer than this, use fresher stock solutions or add more APS and TEMED. 7.
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Page 13: Omnipage Module Assembly And Sample Loading
Using Precast Gels omniPAGE mini is compatible with all the precast gels available in the market. Simply remove the precast gel from the storage pouch. Gently remove the comb. Keep the Inner module upstand on a flat surface and place the precast gel between the pressure bar and the blue gasket. -
Page 14: Gel Running
3.4 Gel Running 1. Fit the lid and connect to a power supply. 2. Consult Table 8 for details on recommended power supply voltage settings. 3.5 Gel Removal 1. Turn the power supply off when the loading dye reaches the bottom of the gel, sooner if your proteins are below 4Kd in size. -
Page 15: Volumes Required Per Gel
See Table 1 below explaining which percentage of gel to use to separate the sizes of proteins indicated. Table 1 Acrylamide percentages Acrylamide Percentage Separating Resolution 60 - 220 KD 7.5 % 30 - 120 KD 10 % 20 - 75 KD 17 –... -
Page 16: Gel Preparation
4.3 Gel Preparation Prepare gel solutions as per tables below. These give the volumes of solutions from the standard stock solutions. These should be gently mixed avoiding generation of bubbles which will inhibit polymerization by removing free radicals. Table 3 Preparation of the separating gel solution for two 10 x 10cm CVS10D gels using 1 mm spacers. Solution 7.5% 10 %... -
Page 17: Buffer Volume
4.5 Buffer Volume Table 5 Buffer Volumes Buffer Volume CVS10D Minimum – Inner tank is filled to above the wells. Outer Tank is 250ml filled to just flood the bottom of the glass plates. Cooling potential is at a minimum which may affect resolution. 500ml Maximum –... -
Page 18: Preparation Of Denatured Protein Samples For Loading
4.7 Preparation of denatured protein samples for loading The instructions given below are for denatured samples. For Native samples, please consult a laboratory handbook. 1. Prepare the protein samples for loading. The volume of sample depends on the capacity of the wells (See Comb specifications section 6.2). -
Page 19: Stock Solutions
4.8 Stock Solutions (For SDS PAGE gels) Stock 30% Acrylamide Gel Solution: 30.0 g acrylamide 0.8 g methylene bisacrylamide Distilled Water to 100ml Stock 4 X Resolving Gel Tris (1.5 M Tris HCl pH8.8, 0.4 % SDS) To 110ml Distilled Water add 36.4 g of Tris base Add 8ml of 10 % SDS Adjust pH to 8.8 with 1N HCl Adjust the final volume to 200ml with Distilled Water. -
Page 20: Trouble Shooting Guide
Section 5 Troubleshooting Guide Problem: Sample Preparation Cause Solution Laemmil sample buffer turns yellow Sample buffer too acidic Add Tris base until buffer turns blue again. Sample very viscous High DNA or carbohydrate content Fragment DNA with ultrasonic waves during cell lysis and protein solubilization. - Page 21 Problem: Electrophoresis Cause Solution Current zero or less than expected Tape at the bottom of precast gel Remove tape. and samples do not migrate into gel cassette not removed Insufficient buffer in inner buffer Fill buffer chamber with running buffer. chamber Insufficient buffer in outer buffer Fill inner and outer buffer chambers to ensure...
- Page 22 Problem: Evaluation of Cause Solution Separation Diffuse or broad bands Poor quality acrylamide or bis- Use electrophoresis-grade reagents. acrylamide incomplete Check polymerization conditions. polymerization Old SDS or sample buffer Prepare fresh solutions. Gel temperature too high Use external cooling during run or run out a lower voltage.
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Page 23: Product Information
Section 6 Product Information 6.1 Catalogue numbers and product descriptions Catalogue No. Product description omniPAGE Mini, 10 x 10cm Dual, 2 sets of Glass Plates, 1mm thick bonded Spacers, 2 x 12 CVS10D sample, 1mm thick combs. CLAMP VERSION omniPAGE Mini, 10 x 10cm Dual, 2 sets of Glass Plates, 1mm thick bonded Spacers, 2 x 12 CVS10DSYS sample, 1mm thick combs including caster. -
Page 24: Combs
VS10S1 10cm Spacers - 1mm thick (pk/2) VS10S1.5 10cm Spacers - 1.5mm thick (pk/2) VS10S2 10cm Spacers - 2mm thick (pk/2) RPW-0.2 Replacement Platinum Wire - 0.2mm, 50cm Multiple Minigel Casting CSL-6CAST 6 gel caster for 8 x 10cm or 10 x 10cm gels CSL-12CAST 12 gel caster for 8 x 10cm or 10 x 10cm gels CSL-24CAST... - Page 25 VS10-16MC-1.5 Comb 16 sample MC, 1.5mm thick VS10-20-1.5 Comb 20 sample, 1.5mm thick 1300 VS10-1-2 Comb 1 Prep, 1 Marker, 2mm thick VS10-5-2 Comb 5 sample, 2mm thick VS10-8MC-2 Comb 8 sample MC, 2mm thick VS10-9-2 2 Comb 9 sample, 2mm thick 100 VS10-10-2 Comb 10 sample, 2mm thick VS10-12-2...
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Page 26: Catalogue Numbers And Product Descriptions For Csl Related Products
6.3 Catalogue numbers and product descriptions for CSL Related Products Catalogue No. Product description CSL-PPL CSL Pink Plus Prestained Protein Ladder, 10-175kDa, with 10, 40 & 90kDa reference bands, 1x 500μL vial. CSL-BBL CSL BLUE Wide Range Prestained Protein Ladder, 10-245kDa, with 25 & 75kDa reference bands, 1x 500μL vial. -
Page 27: Warranty
Section 7 Warranty The Cleaver Scientific Ltd. (CSL) Electrophoresis units have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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