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Cellulose Acetate
Tank
Instruction Manual
Catalogue Numbers
CSL-CELLAS
27/05/2020
CSL-CELLASMINI
Record the following for your records:
Model _____________________
Catalogue No. _____________________
Date of Delivery _____________________
Warranty Period _____________________
Serial No. _____________________
Invoice No. _____________________
Purchase Order No. _____________________
Page 1
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Table of Contents
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Summary of Contents for Cleaver Scientific CSL-CELLAS
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Page 1: Instruction Manual
Cellulose Acetate Tank Instruction Manual Catalogue Numbers CSL-CELLAS CSL-CELLASMINI Record the following for your records: Model _____________________ Catalogue No. _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No. _____________________ Invoice No. _____________________ Purchase Order No. _____________________ 27/05/2020 Page 1... -
Page 2: Table Of Contents
Contents Instruction Manual Catalogue Numbers Contents Safety Information Packing List Operating Instructions Usage Guidance and restrictions Setting up the Cellulose Acetate Tanks Fitting Electrode cables Preparation and Gel running Example of Staining Example of Solutions Care and Maintenance Cleaning Cellulose Acetate Units RNAse Decontamination Warranty 27/05/2020... -
Page 3: Safety Information
Safety Information When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly. -
Page 4: Packing List
The unit should be checked for damage when received. Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue. -
Page 5: Operating Instructions
Operating Instructions Usage Guidance and restrictions • Maximum altitude 2,000m. • Temperature range between 4°C and 65°C. • Maximum relative humidity 80% for temperatures up to 31 C decreas- ing linearly to 50% relative humidity at 40 • Not for outdoor Use. This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664. -
Page 6: Preparation And Gel Running
11 for details on commonly used buffers. Fill the tank with 180ml of buffer per chamber side, 360ml in total for the CSL-CELLAS unit and 100ml of buffer per chamber side, 200ml in total for CSL-CELLASMINI Holding the strip by the edges only in gloved hands, blot the strip in between two pieces of filter paper. - Page 7 Place the strip on top of the wicks and between the bars with the sample end at the negative electrode. Make sure there is good con- tact by pushing down on the ends of the strip. Glass slides can be placed over each end to further ensure a good contact.
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Page 8: Example Of Staining
Example of Staining Ponceau S 1. Immerse the strips in about 50 ml of Ponceau S staining solution for 5 to 10 min. (Amidoblack may be used if preferred) with agitation. 2. Remove Ponceau Example of Solutions Running Buffer • Tris Hippurate 0.05 M, pH 8.8 •... -
Page 9: Rnase Decontamination
RNAse Decontamination This can be performed using the following protocol: Clean the units with a mild detergent as described above. Wash with 3% hydrogen peroxide (H2O2) for 10 minutes. Rinsed with 0.1% DEPC-(diethyl pyro carbonate) treated distilled water, Caution: DEPC is a suspected carcinogen. Always take the necessary pre- cautions when using. -
Page 10: Warranty
Warranty The Cleaver Scientific Ltd. (CSL) units have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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