Related Manuals for BioCare Medical Decloaking Chamber Pro
Summary of Contents for BioCare Medical Decloaking Chamber Pro
- Page 1 Decloaking Chamber Pro Manual ™ Digital programmable pressure system for heat-induced epitope retrieval C H A M B E R DISPLAY START STOP www.biocare.net U.S. patent number 6,580,056...
- Page 2 Biocare Medical Technical Support: 800-799-9499 Option 3 Customer Service: 800-799-9499 Fax: 925-603-8080 Corporate Office: 60 Berry Drive Pacheco CA 94553 www.biocare.net...
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Page 3: Table Of Contents
To the extent permitted by law, Biocare Medical disclaims any liability for any incidental or consequential damages related to this equipment or for any warranty... -
Page 4: Specifications
Specifications Decloaking Chamber The Decloaking Chamber is a programmable bench top pressure cooker intended for laboratory use. It is programmed to allow the precise pressurized heating necessary for antigen retrieval and also has the capability to perform at a variety of temperatures ranging from 37 °C to 125 °C. -
Page 5: Assembly
Assembly Unpackaging Upon receipt of the Decloaking Chamber, inspect the packaging and become familiar with the parts. Save the packaging in case returning the instrument becomes necessary. You should have: • Lid with pressure gauge and hot sticker • Gasket (removable) •... -
Page 6: Description Of Parts
Description of Parts Gasket: The rubber seal between the pan and the lid that ensures an airtight chamber. Cat. No. DC037 Pan: The main removable metal chamber that is placed within the outer shell. The pan comes in contact with the heat sensor and heat plate. Vent Nozzle: The top center of the chamber lid that where the weight (petcock) is placed. -
Page 7: Related Parts / Products
The pH of retrieval solutions at high temperatures can be critical for proper IHC staining. However, the pH of retrieval solutions can change at high temperatures. To assure quality control and consistent staining, Biocare Medical has developed heat-tested strips that give accurate and true pH at high temperatures. -
Page 8: Operating Instructions
If you are facing the Decloaking Chamber, the on/off switch is located to the right of the display panel on the front. It will illuminate red if the instrument is turned on. Recommended Protocols Recommended protocols for HIER with Decloaking Chamber Pro: The protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. - Page 9 Operating Instructions (continued) Place the gasket into the groove on the top of the pan. Place the heat shield in the center of the pan at the bottom. Add 500 ml of DI water.
- Page 10 Operating Instructions (continued) Place pan into the body of the instrument. Place the container with the retrieval solution on top of the heat shield. Do not place the container on the center of the heat shield where the heat is concentrated, but towards the edge of the heat shield.
- Page 11 Operating Instructions (continued) Place the weight (petcock) onto the vent nozzle. Make sure there is a snug, flat connection. Open Match the word "OPEN" on the lid to the white dot on the handle. White Dot Secure lid by turning clockwise until the word "CLOSED" is matched with the white dot on the handle.
- Page 12 Operating Instructions (continued) No Gap Correctly secured lid. Gap indicates that the lid is not correctly secured.
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Page 13: Programming Display
Programming Display Control Panel (See the inside back cover for a recommended settings guide) Heat On light: Indicates that the heating element is heating up. Set light: START DISPLAY Indicates that the current parameter, SP1, SP2 or SP Limit can STOP be programmed. -
Page 14: Default Settings
Default Settings When the main power switch is turned on, the temperature for SP (Set Point) 1 is displayed. If you press the "Display Set" button once, the time for SP1 is displayed. Press the "Display Set" button again and the temperature setting for SP2 displays. Pressing the "Display Set"... -
Page 15: Changing The Factory Settings
Changing the Factory Settings • Set the SP1 temperature and time by pressing the "Display Set" button until the SP1 LED light comes on. Use the up or down arrows to adjust the temperature to the desired retrieval temperature. Press the "Display Set" button once and the SP1 time will display. -
Page 16: Maintenance
Maintenance Daily Pour the water out of the pan, rinse it with deionized water and dry. Inspect the gasket for wear. The lid may be left atop the chamber, but should be slightly ajar. Monthly Preventative maintenance should be done once a month. Unplug the instrument before cleaning. Use only a mild dish detergent, as abrasive powders or metal scrubbers will damage the surface and may cause contamination. -
Page 17: Decloaking Chamber Short Instructions
Short Instructions • Plug the Decloaking Chamber into a 110-120 V power source with surge protection. • Place the gasket in the lip of the Decloaking Chamber lid. • Place the Decloaking Chamber pan into the Decloaking Chamber shell (body). •... -
Page 18: Operating Notes / Important Safety Guidelinesv
Operating Notes / Important Safety Guidelines The Decloaking Chamber has been designed with many unique safety features. However, when using heating and electrical equip- ment, basic precautions should be used. This is a heating device under pressure. Please read these operating notes before operating the instrument. -
Page 19: Cycling Through The Programmable Display
Cycling Through the Programmable Display Step 2 Step 1 START START DISPLAY DISPLAY STOP STOP Turn on the Decloaking Chamber. The current setting Pressing the "Display Set" button once will cause the for the SP1 temperature will be shown on the SP1 time to display. - Page 20 800.799.9499 60 Berry Drive www.biocare.net Pacheco CA 94553 904-016-071817...
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