Related Manuals for BioCare Medical Decloaking Chamber NxGen
Summary of Contents for BioCare Medical Decloaking Chamber NxGen
- Page 1 Decloaking Chamber NxGen Manual ™ Digital programmable pressure system for heat-induced epitope retrieval www.biocare.net U.S. patent number 6,580,056...
- Page 2 Biocare Medical Technical Support: 800-799-9499 Option 3 Customer Service: 800-799-9499 Fax: 925-603-8080 Corporate Office: 60 Berry Drive Pacheco CA 94553 www.biocare.net...
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Page 3: Table Of Contents
Table of Contents Overview ....................................1 Specifications ..................................1 Intended Use ................................1 Transportation ................................2 Storage ..................................2 Handling ..................................2 Operating Environment ..............................2 Power Requirements ..............................2 USB Flash Drive ................................2 Waste Disposal ................................2 Instrument Disposal ............................... 2 Accuracy .................................. -
Page 4: Overview
NxGen offers a walk-away capability similar to fully automated staining instruments. The Decloaking Chamber NxGen transfers run data to a USB drive for export to a user’s computer. The run data recorded includes the date and time per run with temperature and pressure readings throughout. With the Decloaking Chamber NxGen retaining the settings from the last run, a quick start of the same protocol is possible. -
Page 5: Transportation
USB Flash Drive The Decloaking Chamber NxGen is equipped with a USB flash drive that will create and save a data file for every run. The USB flash drive feature requires the use of a USB 2.0 flash drive with a minimum capacity of 512 MB and a maximum capacity of 4 GB. -
Page 6: Parts & Features
Parts & Features 1. Pressure Limit Valve 2. Pressure Poppit 3. Sealing Ring 4. Metal Water Chamber 5. Condensation Collector 6. Handle 7. Outer Body 8. Heating Plate 9. Base 10. ON / OFF Switch 11. Power Cord 12. Lid Handle 13. -
Page 7: Important Safeguards
3. Never attempt to open lid while the unit is greater than or equal to 95ºC. Do not open the Decloaking Chamber NxGen until the unit has cooled to below 95ºC and all internal pressure has been released. If lid is difficult to rotate, this indicates that the chamber is still pressurized. Do not force it to open. Any pressure in the chamber can be hazardous. -
Page 8: Before First Use
Thoroughly clean the lid and the metal water chamber. 1. Place the Decloaking Chamber NxGen on a clean, flat surface where you intend to use it. Position the unit with easy access to the plug for emergency disconnect action in case of malfunction. -
Page 9: Instructions For Use
Once the Decloaking Chamber NxGen is appropriately set up, antigen retrieval can begin. 1. If not already plugged in, plug the power cord first into the Decloaking Chamber NxGen and then into an appropriate wall outlet. Turn ON / OFF switch to the ON position (|). -
Page 10: Control Panel Programming
USB or pre-run screen. Skip Steps 2-4 and go directly to Step 5. 2. If the current run will be different than the last run or the Decloaking Chamber NxGen is being used for the first time, press the menu button to begin the protocol selection process. - Page 11 Control Panel Programming (continued) 6. When the pre-run screen is displayed, review temperature and time selections before starting the protocol. a. Ensure that there is 500ml of deionized or filtered water in the water chamber, the metal rack holder and 3 metal slide canisters are present with solution, the lid is securely fastened, the pressure cable is connected, and the pressure limit valve is attached and level.
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Page 12: Program Completion
2. For protocols below 95ºC, the unit will begin beeping and the current unit temperature will begin flashing upon run time completion. 3. Press the circle button to silence the beeping. 4. The Decloaking Chamber NxGen will then show a Liquid Too Warm screen This screen shows the current temperature of the water chamber. -
Page 13: 95°C Protocol Function
95°C Protocol Function The Decloaking Chamber NxGen has the ability to preheat retrieval solutions when the 95°C protocol is selected. The preheat functionality temporarily pauses the unit when the target temperature is reached, allowing the user to add deparaffinized slides to retrieval buffers that have already been warmed to 95°C. -
Page 14: Reporting Software
Decloaking Chamber NxGen systems, run completion summaries can be provided from a single instrument or all associated units at once. Decloaking Chamber NxGen run files are stored on the computer’s hard drive after upload from the USB Flash Drive provided with each instrument. - Page 15 1. Click on the Summary Report by Instrument option. 2. In the “Serial” option window, select the serial number of the desired Decloaking Chamber NxGen from the drop down list. Alternatively, leave the Serial option set to “All” to generate a Summary List of all uploaded Decloaking Chamber NxGen run files.
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Page 16: Maintenance
3. Remove the condensation collector and empty of any accumulated water. 4. Leave lid next to unit or slightly ajar atop unit. 5. Do not leave liquids in the Decloaking Chamber NxGen when not in use. 6. Wash the metal slide canisters in soap and water. Do not use bleach. -
Page 17: Troubleshooting Guide
Troubleshooting Guide Symptom Possible Reasons Solutions Lid does not lock The sealing ring is not properly installed Reinstall the ring Cannot open the lid after a run The pressure is too high Wait until the pressure drops No sealing ring was installed Install the sealing ring Residue on sealing ring Clean sealing ring... -
Page 18: Quick Instruction Guides
Quick Instruction Guides Instrument Operation 1. Plug in the Decloaking Chamber NxGen and turn ON / OFF switch to the ON position (|). 2. Add 500ml of deionized or filtered water and a metal rack holder to the water chamber. -
Page 19: Additional Consumables
Metal Slide Canister The Decloaking Chamber NxGen is supplied with 3 metal slide canisters. Metal canisters allow for ideal heat transfer and are the recommended container for holding slides in the Decloaking Chamber NxGen. Do not use plastic or glass slide holders. -
Page 20: Symbol Glossary
CE Marking Hot Surface Manufacturer Serial number Consult instructions for use Caution, consult accompanying documents Power Authorized representative European Community USB connector ETL Approved Power socket Please contact Biocare Medical Technical Support if any problems arise: 800-799-9499 Option 3. - Page 24 800.799.9499 60 Berry Drive Pacheco CA 94553 www.biocare.net 904-012-071817...
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