Related Manuals for BioCare Medical Decloaking Chamber Plus
Summary of Contents for BioCare Medical Decloaking Chamber Plus
- Page 1 Decloaking Chamber Plus Manual ™ Digital programmable pressure system for heat-induced epitope retrieval ° 125.0 00:00:30 P1 SET HH:MM:SS START DISPLAY STOP www.biocare.net U.S. patent number 6,580,056...
- Page 2 Biocare Medical Technical Support: 800-799-9499 Option 3 Customer Service: 800-799-9499 Fax: 925-603-8080 Corporate Office: 60 Berry Drive Pacheco CA 94553 www.biocare.net...
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Page 3: Table Of Contents
To the extent permitted by law, Biocare Medical disclaims any liability for any incidental or consequential damages related to this equipment or for any warranty... -
Page 4: Overview
Overview The new and improved Decloaking Chamber Plus has been redesigned with a programmable walk-away capability, a cooling fan for more rapid antigen retrieval and an optional stainless steel rack holder for plastic slide containers. Furthermore, a USB port enables the laboratory to utilize a flash drive to track and save run data for quality control purposes. -
Page 5: Transportation, Storage And Handling
Unpacking should be performed or supervised by trained laboratory personnel or facility engineer who will record any issues with regard to missing or damaged contents. In the event the instrument is damaged in transit, do not operate and contact Biocare Medical for instructions. -
Page 6: Description Of Parts
Description of Parts Rack Holder A circular stainless steel basket, capable of holding plastic slide The main removable metal chamber that is placed within the containers. outer shell. The pan comes in contact with the heat sensor and Cat. No. RMDC085 heat plate. -
Page 7: Related Consumables
USB flash drive The Decloaking Chamber Plus is equipped with a USB flash drive feature that will create and save a data file for every run. The USB flash drive feature requires the use of a USB 2.0 flash drive with a minimum capacity of 512 MB. -
Page 8: Important Notes
Important Notes The Decloaking Chamber has been designed with many unique safety features. However, when using heating and electrical equipment, basic precautions should be used. This is a heating device under pressure. Please read these notes before operating the instrument. Lift the lid gently When operating, make sure the lid is in the closed position. -
Page 9: Important Safety Guidelines
Important Safety Guidelines The Decloaking Chamber has been designed with many unique safety features. However, when using heating and electrical equipment, basic precautions should be used. This is a heating device under pressure. Please read these safety guidelines before operating the instrument. -
Page 10: Recommended Protocols
For elevation greater than 2000 M, time duration may need to be adjusted. Recommended Protocols Recommended protocols for HIER with Decloaking Chamber Plus: The protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. -
Page 11: Programming
Operating Instructions (continued) Standard Programming Turn the instrument on by flipping the red toggle to the right of the control panel. Press and hold the "Display Set" button for 3 or more seconds to access the program-mode. To verify or change your program, follow instructions in “Default Settings” or “Changing the Default Settings” (page 13). To exit the program-mode, press and hold the "Display Set"... - Page 12 Operating Instructions (continued) 1) Check that the gasket has already been placed into the groove on the top of the pan. 2) Add 500 ml of DI water to the pan. 3) Place pan and rack holder into the body of the instrument. Note: If rack holder is not available, place the heat shield in the bottom center of the pan.
- Page 13 Operating Instructions (continued) 5) Place steam monitor strip across the retrieval solution container. 5a) Unused Steam Monitor Strip. 5b) Steam Monitor Strip after being exposed to the temperature of 110 °C or above for 30 seconds. 6) Place the weight (petcock) onto the vent nozzle. Making sure it is fully seated and rotates freely.
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Page 14: Positioning The Decloaking Chamber For Emergency Disconnection
Operating Instructions (continued) No Gap 9) Correctly secured lid. 10) Gap indicates that the lid is not correctly secured. Positioning the Decloaking Chamber for Emergency Disconnection If you are facing the Decloaking Chamber, the on/off switch is located to the right of the display panel on the front. It will illuminate red if the instrument is turned on. -
Page 15: Programming Display
Programming Display Control Panel LED Screen: Displays the current setting or error message. Display Set Button: Used for accessing and changing the temperature mode and time. ° 125.0 00:00:30 Up Button: P1 SET HH:MM:SS Used for increasing the temperature or time setting. Down Button: Used for decreasing the temperature or time setting. -
Page 16: Default Settings
Programming Display (continued) Audible Alerts Preheat: 3 beeps will sound when the set-point temperature is reached. End of Run: The instrument will beep for 1 minute, indicating that the run is complete. To turn off the beeping tone at the end of the run, press and hold the Start/Stop button for 3 seconds. -
Page 17: Usb Flash Drive Feature
USB Flash Drive Feature The USB flash drive feature (flash drive not included) of the Decloaking Chamber Plus creates and saves data files for every run, logging time, temperature and process events at one minute intervals for the entire run. The data file also includes the system date and time and an unique file number for every run. -
Page 18: Start Program
USB Flash Drive Feature (continued) Start Program Turn on power. Insert the USB flash drive and allow 30 seconds to initialize before starting the program. Press the START/STOP button. If the Delayed Start feature is enabled the screen will display “DELAYED START” and count down the time to the start of the program. -
Page 19: Run Report
USB Flash Drive Feature (continued) Run Report The data file created on the Decloaking Chamber can be downloaded to a computer using Microsoft (MS) Office Excel. This will create a report that can be saved or manipulated as any Excel file. To download the data files follow these steps: Remove the USB drive from the Decloaking Chamber and insert into an available USB port on a computer with MS Excel installed. -
Page 20: Maintenance
Maintenance Daily Pour the water out of the pan, rinse it with deionized water and dry. Inspect the gasket for wear. The lid may be left atop the chamber, but should be slightly ajar. Monthly Preventative maintenance should be done once a month. Unplug the instrument before cleaning. Use only a mild dish detergent, as abrasive powders or metal scrubbers will damage the surface and may cause contamination. -
Page 21: Quick Instructions
Quick Instructions Assembly and Setup 1. Plug the Decloaking Chamber into appropriate power source with surge protection. 2. Check that the gasket has already been placed into the groove on the top of the pan. 3. Place the Decloaking Chamber pan into the Decloaking Chamber shell (body). Pour 500 ml of deionized (DI) water into the pan. Place rack holder into the pan. -
Page 22: After Run Completion
Quick Instructions (continued) After Run Completion Confirm that the “Cycle Done!” message is displayed. Visually confirm that the pressure has dropped to 0 (zero) psi. Turn the unit off. Toggle the weight (petcock) to release any residual pressure. Open the lid with the steam directed away from yourself. Confirm that run has completed successfully by the following: a. -
Page 23: Symbol Glossary
If, for any reason, the Decloaking Chamber fails to function correctly, first double check the power connection; then check the program for correct display of operating parameters. There are no user serviceable parts, only qualified trained personnel can service this equipment. Please contact Biocare Medical Technical Support if any problems arise: 800-799-9499 Option 3. - Page 24 800.799.9499 60 Berry Drive Pacheco CA 94553 www.biocare.net 904-003-071817...