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Summary of Contents for Benchmark Scientific Accuris MyGel InstaView
- Page 1 MyGel™ InstaView Electrophoresis Tank Operating Manual Version 1.0...
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Page 2: Table Of Contents
Keep this manual for future reference. Please check the list of included components in section 1.1, and if there are any parts missing, damaged, or incorrect, please contact your distributor or Benchmark Scientific’s Customer Service Department at 908-769-5555 or by email at info@benchmarkscientific.com. -
Page 3: Included Component List
The MyGel InstaView Gel Tank is a horizontal electrophoresis system that includes a gel running tank, safety lid with viewing filter, blue LED transilluminator, and a gel casting set. The system is designed to connect to an appropriate electrophoresis power supply for separating nucleic acids in agarose gels, and allows viewing the stained DNA bands during electrophoresis without removing the gel from the tank. -
Page 4: Diagrams Of Included Components
1.2 Diagrams of Included Components Smart phone imaging enclosure with photo filter Safety Lid with Integral Viewing Filter and Power Leads Gel Running Tank Blue LED Transilluminator 12V Power Supply... - Page 5 Diagram of Included Components (Gel Casting Set Double Sided Comb (12/22 teeth) Short Gel Trays (10.5 x 6cm) Long Gel Tray (10.5 x 10cm) Gel Casting Divider Gel Casting Stand...
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Page 6: Specifications
1.3 Product Specifications Migration Tank Dimension 120mm×110mm×45mm（inner dimension） Buffer volume 200ml-225ml Electrodes Platinum wire Blue LED Transilluminator Viewing area 10.5cm x 10.5cm dimension Wavelength 465nm 12VDC (Power Adapter included for 100VAC to Input voltage 240VAC input) Gel Casting Set Accommodates 10.5cm x 11cm and Gel casting stand 10.5cm×6cm trays (Divider included) Teeth width x Teeth thickness x Teeth no.）... -
Page 7: Installation
Do not attempt to open or repair any parts of the system. Contact your distributor or Benchmark Scientific for service. Always use the MyGel InstaView in an environment with low humidity and low dust, also keep away from water, direct sunlight / strong light, corrosive gas, high magnetic fields, heaters, fires and other heat sources. -
Page 8: Gel Preparation
4. Gel Preparation Place the gel casting stand on a level surface, and place the divider and desired gel trays into the proper positions in the stand. Either one long gel tray, or two short trays can be inserted into the casting stand (see diagrams on right). - Page 9 Note: After mixing and heating agarose solution, let it cool to approximately 60°C before pouring into the gel trays Pour the agaose gel/DNA stain solution into the gel tray to make a gel approximately 4mm thick. Each 6x10.5cm tray requires about 25ml of agarose.
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Page 10: Gel Tank Set Up
5. Gel Tank Set Up Note: Make sure that the MyGel System is installed on a stable and level surface. This will ensure even sample migration. Place a prepared gel, together with the gel tray onto the gel bed inside the gel tank. Carefully pour an appropriate buffer solution to a level of about 2 mm above the surface of the gel. - Page 11 Add DNA samples to the gel Use an appropriate pipette to carefully dispense samples into the wells of the gel Note: Gel loading buffer should be mixed with the DNA samples so they will sink to the bottom of the wells. Carefully lower the safety cover onto the gel tank.
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Page 12: Electrophoresis
6. Electrophoresis Connect the positive (red) and negative (black) power leads from the myGel Connect the safety cover to the + and – outputs of a 12V power power supply. supply to the Set the power supply output voltage transilluminator appropriately for the application. -
Page 13: Imaging Using A Smart Phone
8. Imaging using a smart phone To take a picture of the gel using a smart phone camera, fit the imaging enclosure onto the lid of the gel running tank. Insert the included orange photo filter into the top platform of the enclosure. Turn on the smart phone’s camera application, and align the camera lens with the photo filter. -
Page 14: Troubleshooting
10. Troubleshooting Problem Causes Resolutions Check the proper operation of the Power supply not power supply and proper connection operating or not No Migration of the power leads to + and - outputs. correctly connected or Check that the gel is properly oriented gel inserted backwards (DNA will migrate towards + electrode) Abnormal... - Page 15 myGel InstaView Accessories and Power Supplies: Gel Casting Accessories Extra casting set, includes casting stand, divider, 2 trays for 10.5x6cm E1200-CS1 gels, one tray for 10.5x10cm gels, 2 double sided combs (12/22 teeth) E1101-COMB1 Extra double sided combs, 12/22 teeth, pack of 2 Blue Light Viewing Accessories E4000-VG1 SmartBlue Viewing Glasses...
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