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of activity should be kept to a minimum
while the cabinet is in use.
4.
Avoid using floor-type pipette discard
canisters. It is important that your used
pipettes be discarded onto a tray or other
suitable container inside the cabinet. This
reduces the temptation to move in and out of
the work area unnecessarily.
Because of tl1e restricted access, pipetting
within the cabinet will require the use of
pipett:ing aids.
5.
Use good aseptic technique. Procedures done
with good technique and proper cabinet
methods will not require the use of a flame.
If, however, a safety officer approves the use
of flame after evaluating the circumstances,
then a burner with a pilot ljght such as the
"Touch-0-Matic" should be used. Place it at
the rear of the work area where the air
turbulence caused by the flame wilt have the
least possible effect. Flame disturbs the
directional airstream and also contributes to
tile heat load.
If
the cabinet blower is
unintentionally turned off, the flame could
also damage a filter.
Tubing for a burner within the cabinet should
be resistant to cracking or pU11cture. Material
such as Tygon tubing may not be acceptable
for this use.
6.
Never operate your cabinet while the
viezvscreen alarm iHdicator is on. The
operating position of the sash provides an 8-
inch high access opening. This restricted
opening permits optimum operating
cuHditiou:; foL the cabinet. For operati.ttg
comfort it is recorrunended that the top of the
operator's shoulder be at the same height as
the bottom of the viewscreen. Because
operators wili not
an
be the same height, it is
suggested that the operator use a chair which
may be adjusted for height.
7.
~-\fter
a procedure has been cornpleted, all
equipn1ent 1-vhich has been !_n contact \Vith
the research agent should be enclosed, and
the entire surface decontarninated. Trays
of
discarded pipettes artd glassv..'are should be
covered. The cabinet should then be allowed
14
to nm for at least three minutes with no
activity so that the airborne contaminants will
be purged from tbe work area. Next, make
sure that all equipment is removed from the
cabinet.
8.
After you have removed all materials, culture
apparatus, etc., decontamination of the
interior surfaces should be repeated. Check
the work area carefully for spilled or splashed
nutrient which might support bacterial
growth. Never use the cabinet to store
supplies or laboratory equipment.
9.
We recommend that the cabinet be left
numing continuously to ensure containment
and cleanliness. If the user elects to turn the
cabinet off at the end of a work session, the
viewscreen should be closed completely. The
sash alaml will be silenced when the
viewscreen is in the closed position.
Reacting
to
Spills
1.
If an accident occurs which causes spills and
spatters arolll1d the work area, you will need
to decontaminate all items and surfaces
before any items are removed.
If
the spill
was enough to create puddles of liquid in the
drain pan, then an emergency spiU procedure
should be followed. (This procedure should
be established prior to an accident.)
It is recommended that the researchers, in
coordination with their consulting safety
professional, have a written plan available in
case of an accidental exposure or spill. The
safety p!Bn shoHld include all of the
ernergency procedu1es to be folloYved in
t}'te
event of an accident. All employees should
be familiar with the emergency procedures.
The emergencv spill procedure
may
vary
accord.ing to the agents being used.
2.
In the case of a biological spill, for example,
the area containing the spi_U n1ay be flooded
with an appropriate disinfectant. The drain
capacity of the
SG400
rnodels is 19.S gallons,
with the
SG600
mode.\s having a
28.7
gal.lon
capacity.
iJter
the disirlfectant has had tllne for a
complete kill, remove or drain tbe residue. If
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