Ii - Preparing The Sterilgard®Ii Cabinet For Use; Checking The Cabinet On Arrival; The Uses Of A Biological Safety Cabinet; Cautions - Baker SterilGARD II SG400STD Operator's Manual

II - PREPARING THE STERILGARD®II CABINET FOR USE
Checking the Cabinet On
Arrival
Upon receipt of your new SteriiGARDoll
cabinet, first inspect the exterior of the crate and
skid. If there is any broken glass or other visible
damage, that fact should be noted on the
receiving slip and immediately reported to the
delivering carrier.
Now remove the crate and inspect the tmit
itself. The top cover of the crate should be taken
off first, then the boards from front and back.
Bend both ends of the crate outward away from
the sides of the unit and remove front or rear
blocking from the skid. Remove the cabinet from
the skid with a fork lift or other available
equipment. If any concealed damage is found it
should be reported to the delivering carrier, who
will want
m1
opportunity to inspect the damage.
A claim for restihttion shollld be filed within 15
days.
. Because of the dwger of mishandling by
trucking companies, we have removed certain
parts of the cabinet and have packed them
separatelv. These items are listed on the packing
slip which accompanies the unit. Please check the
packing slip carefully to be sure that all items
have been located.
The
Uses
of a Biological Safety Cabinet
The SterilGARD"II cabinet has been designed to
provide a work area which protects the
experiment from the environment, and the
enviroruncnt frorn the experi-ment. The larninnr
flow biological safety cabinet is designed for work
with Biosafety Levels 1, 2 and 3 (low to moderate
risk) agents as listed in The Center for Disease
Control's
~~Biosafety
in lviicTobiological and
Biomedical Laboratories", U.S. Department of
Health and Hllman Services, Public I-Iealth
Service, Centers for Disease Control and National
Institutes of Health, L.S. Govenunent Printing
Office, Washington, D.C. 20402. HHS publication
nurnber (CDC! 34.-3395.
Biosafety level 4 or extremely f'Ligh risk agents
sl1ould neve·r be used in ul-lls cabinet. Please
5
consult your safety professional for a proper risk
assessment.
C4.UTIONS
*
T1w use of any hazardous material in the
cabinet requires that it be monitored by an
industrial hygienist, safety officer or other
qualified i>u/ividual.
* Explosive or flammable su/Jstances should
never be used in the cabinet unless a quali-
fied safety professional has evaluated the
risk.
* lf hazardous biological work is to be per-
formed, apply the app1·opriate biohazard de-
cal which is enclosed. This is in accord with
OSHA
regulations, volume
39,
11umber
125,
PadiL
* If chentical, 1·adialogical or other n.on.micro-
bialogical hazards are present, be sure to
employ appropriate protective measures in
addition to formaldehyde decontamination.
Call upol! a suitably trailred individual to
monitor the operation.
Location Within the Laboratory
The ideal location for any laminar flow
biological safety cabinet is in a dead-end comer of
the laboratory away from personnel traffic, vents,
doors, windows and any other sources of
dismptive air currents. Published research from
The Baker Companv (Rake, B.W. "Influence of
Crossdrafts" on the Perfonnance of a Biological
Safety Cabinet. Appl. Microbiology 36:278-283,
1978.) and w1published tests performed at the
National Cancer Institute show that i.f a draft or
other disruptive air current were to exceed the
intake velocity of the cabinet, then contarrlination
can e-nter the vvork area or escape
frorn
it. Proper
placement within the is essential.
If the cabinet exhausts Lts air into the Iaborrltonr
ins:tead of venting to the
OL.ltsidef
it is
imoortant
that there be adequate space between
th~
top
of
t._he cabinet and the ceiling. A soJjd ceiling located