Sequoia LISST-200X User Manual
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Sequoia LISST-200X User Manual

Particle size analyzer including lisst-hab & lisst-black
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LISST-200X
Particle Size Analyzer
Including
LISST-HAB & LISST-Black
User's Manual
Version 2.0
October 2020
Store Software
USB Card Here
2700 Richards Road, Suite 107
Bellevue, WA 98005-4200
Tel: (425) 641-0944 Fax: (425) 643-0595

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  • Page 1 LISST-200X Particle Size Analyzer Including LISST-HAB & LISST-Black User’s Manual Version 2.0 October 2020 Store Software USB Card Here 2700 Richards Road, Suite 107 Bellevue, WA 98005-4200 Tel: (425) 641-0944 Fax: (425) 643-0595...
  • Page 3 © This document is copyrighted by SEQUOIA SCIENTFIC, INC LISST-200X User’s Manual Page iii...
  • Page 5 This manual is divided into two sections. I. LISST-200X contains an introduction to the LISST-200X instrument and the principles of its operation. II. LISST-200X Operation provides a detailed set of instructions for using and caring for the instrument. Technical assistance For technical assistance please contact your local Distributor or Sequoia.

  • Page 7: Table Of Contents

    View Processed Data File ..................51 Data Quality Control ....................52 Simple Real-Time Data Processing ................. 54 Collecting Data from an External Analog Sensor ............. 56 Configuring the LISST-200X as a Sensor for a CTD ..........57 ..................... 58 NSTRUMENT OMMUNICATION LISST-200X C ..................
  • Page 8 LISST-200X User’s Manual Table of Contents Page viii...

  • Page 9: Lisst-200X Introduction And Tutorial

    (PSD). That is the essence of this method. In order that you appreciate the importance of some steps in operation of the LISST-200X, we recommend reading the rest of this chapter. The measurement of scattering at multiple angles is done with a collimated beam illuminating particles in water.
  • Page 10 A factory background acts as a reference to compare with, to identify degradation in laser output or fouling of optical surfaces. The LISST-200X stores its factory background data in its memory, as well as the user’s acquired background data.
  • Page 11 As to which particle model to use, we suggest that when working with natural waters, use the irregularly shaped model. Only in exceptional circumstances, the spherical model is appropriate. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 3...

  • Page 12: Lisst-200X General Description

    Windows software. For MATLAB users, inversion scripts are available for download from Sequoia’s website: www.SequoiaSci.com. The LISST-200X instrument is a laser diffraction device. It consists of optics for producing a collimated laser beam, a specially constructed detector array, electronics for signal pre-amplification and processing, data storage and scheduling computer, and an external battery system.
  • Page 13 A gauge pressure sensor is calibrated to measure the pressure relative to a given atmospheric pressure, as opposed to an absolute pressure sensor, which measures pressure relative to a vacuum. The pressure sensor on the LISST-200X will read 0 at atmospheric pressure.
  • Page 14 Battery Life The LISST-200X does not contain internal batteries (except a small battery to maintain the real-time clock), but can be powered from multiple sources including a USB computer cable, Small or Large Battery Packs (provided with the instrument) or an external instrument such as a CTD.

  • Page 15: Lisst-200X Quick Start Tutorial

    User’s manual following this Quick Start Tutorial. Let’s assume that you are opening the LISST-200X shipping case for Contents of Shipping Case the first time.
  • Page 16 Instrument from a flat working surface. Remove the LISST-200X from the case and set Shipping Case. it on the stands. The LISST-200X has two distinct ends that we will refer to as the Optics endcap and the Connector endcap. Optics Endcap The optics endcap contains the optical windows that the laser beam passes through to make a measurement.
  • Page 17 This magnet can be used to trigger a digital switch inside the instrument which can be programmed to start and stop the LISST-200X sampling. The final item on the endcap is the zinc anode. The sacrificial zinc anode protects the instrument from corrosion during long periods of time in salt water.
  • Page 18 If the driver installed correctly, you should see the green light on the USB cable blinking. For more information on establishing and troubleshooting communication with the LISST-200X see the Step-by-Step guide on page 29 of the User’s Manual.
  • Page 19 We can now fill the chamber with clean, particle-free water. Sequoia uses steam-distilled bottled water filtered through a 0.2 micron filter. Tap water may contain too many particles and may also contain dissolved gas that can release and form small bubbles on the optical surfaces.
  • Page 20 LISST-200X placed on your desktop. Application Step 7: The LISST-200X does not have an internal battery. Power is supplied Establish to the instrument via a battery pack or USB cable. Please note that Communication the LISST-200X USB cable has a USB-to-serial convertor and special...
  • Page 21 Step 8: Open The LISST-200X software has a terminal window that allows the user Terminal to communicate directly with the instrument using a set of commands. window To open the Terminal window click on the connected instrument message in the lower left, then click on the wrench icon and select ‘Open Terminal’...
  • Page 22 High values on the inner rings combined with a lower transmitted laser power value can also be an indication of optical misalignment. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 14...
  • Page 23 If the values are acceptable the values can be saved both onboard the instrument and to a file on your computer. The LISST-200X handles the background files differently than the LISST-100X. The background file is stored on the instrument and is saved as part of every data file that is recorded.
  • Page 24 The icons next to the various values give a better understanding of their meaning. The software automatically checks the values entered to make sure that there is no LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 16...
  • Page 25 For this example let’s select the External Mechanical Switch Start Condition. AutoStart If the AutoStart check box is selected, the LISST-200X will start the sampling program when power is applied to the instrument. If this is not selected the user must select the ‘Run Sample Program’ button from the instrument status bar or send the ‘GO’...
  • Page 26 6.5 V). For this example, choose the External Mechanical Switch as the Stop condition. Step 14: The accessories tab allows you to select which LISST-200X Configure accessories are connected to the instrument. The accessories must be Accessories selected here for them to function properly. If there are no accessories, select ‘None’.
  • Page 27 Instrument you have unsaved changes to the instrument configuration Click on the ‘Upload to Instrument’ button to load the configuration onto the LISST-200X. A confirmation message will be displayed when the upload is finished. Step 16: Start The instrument is now configured for deployment, however, it is not Instrument yet running.
  • Page 28 “1“ position. Sampling will start. Data collection will continue until Collecting Data the Stop Conditions are met. In our case, until the switch lever is returned to the “0” position. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 20...
  • Page 29 Choose the files to offload by clicking on them while holding down the CTRL key. The Shift key can also be used to select a range of files. Choose a location to offload the files to on the right side. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 21...
  • Page 30 Raw data files have the extension .RBN, and are offloaded directly from the LISST- 200X data logger. Below is list of the file types you can expect to see when using the LISST-200X. Extension Discription Format .RBN...
  • Page 31 Processed data files are stored as ASCII files (.CSV). When a raw file Processed is processed the resulting .CSV file is displayed automatically. You can Results also open any LISST-200X .CSV file by selecting the View Process File button from the home page. LISST-200X User’s Manual LISST-200X Introduction and Tutorial...
  • Page 32 Step 21: The LISST-200X software also supports the ability to acquire, process, Opening a Real and display size data it in real time. The Start and Stop conditions will Time Session not be used and therefore their settings are not relevant.
  • Page 33 The above steps should have given you a good understanding on the LISST-200X in how to operate the LISST-200X. More details on all of the steps can be found in the User’s Manual following this tutorial. The following the Field steps go into deploying the instrument in the field.
  • Page 34 We highly recommend using air shipping when possible to eliminate the extended vibrations that ground shipping can cause. Because the LISST-200X does not have an internal battery there is no need to worry about putting the instrument into low power sleep mode.
  • Page 35 When using the Analog Output configuration power can be received through the Auxiliary connector. The wiring of the connector is designed to match the SeaBird CTD Auxiliary Input connector simplifying the connection. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 27...

  • Page 36: Lisst-200X Operation Details

    WARNING The LISST-200X uses a laser diode emitting a maximum of 1 mW of visible (red) light at a wavelength of 670nm. The laser beam under normal circumstances is not a threat. However, if objects are placed in the path of the laser beam, the light could be reflected into the eye causing permanent damage.

  • Page 37: Step By Step Procedures

    D. Step by Step Procedures Installing LISST-200X Software Software for the PC is used to configure the LISST-200X and for downloading and processing the size distributions. STEP ACTION RESULT • The LISST-200X comes with a USB memory card. Installer was found on...

  • Page 38: Establishing Communication With The Lisst-200X

    Establishing Communication with the LISST-200X Establish communication with LISST-200X using the supplied software and USB cable. Communicating with the LISST-200X via the software should be automatic. Should there be a problem you can use these step-by-step instructions to troubleshoot the problem.

  • Page 39: Saving And Evaluating Clean Water Backgrounds

    ACTION RESULT • Connect the instrument to the computer and establish Software open and communicating with communication in the LISST-200X program. (see LISST-200X. Step-by-Step Procedure: Establishing Communication with the LISST-200X) • Clean instrument and install Clean Water Background Optics submerged in water Test chamber.
  • Page 40 If needed you can update the background, such as after cleaning the windows or replacing the water, by pressing the Collect New Background button again. LISST-200X User’s Manual LISST-200X Operation Details Page 32...
  • Page 41 After the background is saved, select the ‘Save as .BGT File’ button. The LISST-200X handles the background files differently than the LISST-100X. The background file is stored on the instrument and is saved as part of every data file that is recorded.

  • Page 42: Configuring Data Collection

    Configuring Data Collection The LISST-200X software can be used to configure the Start and Stop Conditions, Fixed Rate or Burst sampling and sample rates. STEP ACTION RESULT • Connect the instrument to the computer and establish Software open communication in the LISST-200X program. (see Step-by-...
  • Page 43 • There are five options: Depth, Time, External Mechanical Switch, External Digital Input, and Time Delay. Select the mode by clicking on the button next to the its label. Select the correct parameters as required. LISST-200X User’s Manual LISST-200X Operation Details Page 35...
  • Page 44 ‘GO’ command to start the sampling programing after apply power. • NOTE: Auto Start will not occur if the power is connected to LISST-200X in low power sleep mode. • Choose the Stop Conditions Tab to select the conditions Stop Condition when sampling should stop.
  • Page 45 • Choose the Accessories tab to configure LISST-200X accessories. The accessories must be selected here in order for them to function properly. • After configuring the instrument settings, they must be uploaded Upload onto the instrument. A message will be displayed on the top of...
  • Page 46 At this point the user will know that the instrument is running and is ready to be deployed. LISST-200X User’s Manual LISST-200X Operation Details Page 38...

  • Page 47: Using The Small External Battery Pack

    Using the Small External Battery Pack The LISST-200X comes with a Small External Battery Pack that can be used for short term deployments. The instructions in this section describe how to use the Small Battery including installing and charging the NiMH rechargeable batteries.
  • Page 48 Align the thumbscrews with the holes in the main housing and push the endcap on until it is flush with the housing. • Tighten the thumbscrews by hand (DO NOT USE ANY TOOL). DO NOT OVERTIGHTEN. LISST-200X User’s Manual LISST-200X Operation Details Page 40...
  • Page 49 Bulkhead connector on the Small Battery Pack. • Connect the other end of the cable to the 5-pin Male bulkhead on the LISST-200X. • Power is now going to the LISST-200X. If AutoStart is enabled the instrument may be looking for the start condition.

  • Page 50: Using The Large External Battery Pack

    Using the Large External Battery Pack The Large External Battery Pack is used to provide power to the LISST-200X for long term deployments or extended profiling operations. STEP ACTION RESULT • The LISST-200X Large External Battery is shipped Battery ready to use when shipped.
  • Page 51 • Screw the connector endcap onto the pressure case Battery assembled and ready for use. with the four 6-32 x ¾ socket head cap screws. If necessary, apply anti-seize compound onto the threads. LISST-200X User’s Manual LISST-200X Operation Details Page 43...
  • Page 52 “TO LISST”. • Connect the other end of the cable to the 5-pin Male bulkhead on the LISST-200X. • Power is now going to the LISST-200X. If AutoStart is enabled the instrument may be looking for the start condition.
  • Page 53 • If desired connect the Communications cable to the male 5-pin bulkhead connector on the Large Battery Pack which is labeled “TO PC”. Communications will pass through the battery to the LISST-200X. • The battery and communications cables are shown below.

  • Page 54: Offloading And Deleting Data Files From Internal Memory

    Offloading and Deleting Data Files from Internal Memory Data collected using the LISST-200X is stored on the internal data logger. This section describes how to offload and delete files from the internal data logger. STEP ACTION RESULT • Software open and...

  • Page 55: Processing A Single Raw Data File

    Processing a Single Raw Data File Data that has been downloaded from the datalogger is in a raw binary file (.RBN extension). It must be processed into particle size by the LISST-200X program. STEP ACTION RESULT • Start the LISST-200X program Program started.
  • Page 56 Excel etc. • The RTX file is a space delimited ASCII file containing the raw data from the LISST-200X data logger. This is useful for looking at the raw data in a spreadsheet such as Excel etc.

  • Page 57: Batch Processing Multiple Raw Data Files

    Data that has been downloaded from the datalogger is in a raw binary file (.RBN extension). It must be processed into particle size by the LISST-200X program. If you have multiple raw data files to process (e.g. from a series of profiling deployments), follow these processing steps that allows you to process all files in one operation.
  • Page 58 • The CSV file is a space delimited ASCII file containing all the processed data. • The RTX file is a space delimited ASCII file containing the raw data from the LISST-200X data logger. • Press the “Process Files” button.

  • Page 59: View Processed Data File

    STEP ACTION RESULT • Start LISST-200X program Program started. • Select View Processed File from the home page • Select the processed data file (.CSV) from the file Processed data file selected.

  • Page 60: Data Quality Control

    • This means that the signal-to-noise ratio will be low, and the data may have a lot of noise in them, but can most likely still be used. LISST-200X User’s Manual LISST-200X Operation Details Page 52...
  • Page 61 0.98 and 0.995 – low signal-to-noise ratio. • Be wary of data collected at transmission values between 0.30 and 0.10 – generally decreasing data quality as the transmission decreases below 0.30 (30%). LISST-200X User’s Manual LISST-200X Operation Details Page 53...

  • Page 62: Simple Real-Time Data Processing

    STEP ACTION RESULT • Connect the instrument to the computer and establish Communication established. communication in the LISST-200X program. (see Step- by-Step Procedure: Establishing Communication with the LISST-200X) • The background currently stored onboard the instrument Background is current will be used to process the data as it is collected in real time.
  • Page 63 • The CSV file is a space delimited ASCII file containing all the processed data. • The RTX file is a space delimited ASCII file containing the raw data from the LISST-200X data logger. • Data will not be saved until the ‘Start Logging Data’ button Start Data Logging is pressed.

  • Page 64: Collecting Data From An External Analog Sensor

    If using the 12V output, you can specify how long it should turn on before sampling, as a warmup time for the sensor. To provide these functions, the auxiliary connector on the LISST-200X must be in one of three configurations: Analog & Digital Input (which provides a single analog input), Dual Analog Input configuration (which provides two), or Triple Analog Input configuration (which provides three).

  • Page 65: Configuring The Lisst-200X As A Sensor For A Ctd

    For highest-quality data you will still need to offload and process the detailed data from the LISST-200X after deployment. The LISST-200X updates its analog outputs each time it collects a sample, at a maximum of 1 Hz.

  • Page 66: Instrument Communication

    LISST-200X software. A slower speed may be required when downloading data over cables longer than a few meters. Using the LISST- In the LISST-200X software, you can open a terminal window to directly 200X Terminal view communications with the LISST-200X, and enter commands.
  • Page 67 The details of each condition are described below. Depth Start The built-in depth sensor of the LISST-200X is used to check the current depth to determine if the desired start depth has been exceeded. The instrument is powered up and 5 measurements of the depth are averaged over a two-second period.
  • Page 68 Depth Stop The depth sensor of the LISST-200X is used to check the current depth to determine if it is less than the desired Stop depth. If the depth is less than the threshold the sampling will stop. If the Start Condition is Depth Start the program will return to checking for the start depth.
  • Page 69 Memory Full Sampling will continue until the memory is full, or the power input falls. If the memory fills, the instrument will go into deep sleep. LISST-200X User’s Manual LISST-200X Operation Details Page 61...

  • Page 70: Lisst-200X Command Summary

    LISST-200X Command Summary NOTE: Command are shown in upper case for clarity, but are not case-sensitive. Display Commands CONFIG Display instrument hardware configuration information DD, DIR Display current disk directory DEPTH Display current depth Display current instrument status information Display current time and date...
  • Page 71 Start data collection using current parameters Grab sample and transmit it Transmit raw data when average saved Acquire 20 samples and evaluate them for quality. Go into deep sleep mode (minimum power consumption) LISST-200X User’s Manual LISST-200X Operation Details Page 63...

  • Page 72: Lisst-200X Command Details

    G. LISST-200X Command Details Display Commands CONFIG Display instrument hardware configuration Syntax: CONFIG Description: The current hardware configuration including Auxiliary I/O connector configuration Example: input: CONFIG output: Serial Number: 2001 Firmware Version: 1100 (1.100) VCC: 59371 Full Path Length: 2500 (25 mm) Eff.
  • Page 73 Description: The instrument settings and status are displayed to the screen. Example: input: DS output: LISST-200X Status and Settings Serial number = 1004 Endcap Configuration: 0 (Analog & digital in) Firmware Version 1.126 Apr 26 2016 18:45:52 LISST-200X User’s Manual...
  • Page 74 External sensor power during logging: off External sensor warmup time: 0 seconds Free memory: 1023 Mbytes input: DS 1 output: LISST-200X Current Status and Settings SN = 1004 OM = 0 ST = 5 TD = 0 SP = 5...
  • Page 75 Description: Displays the list of command to the screen. Example: input: HE output: LISST-200X Commands (followed by 30 lines of text) Display current Mechanical Switch position Syntax: Description: The current position of the Mechanical Switch is displayed to the screen.

  • Page 76: Setup Commands

    If no value follows command, prompts will be displayed for the value. After setting the start condition to Depth, Time or Delay, use STARTDATA to set the applicable condition value. Example: input: ST 5 output: New Start Condition Setting: Delay Start LISST-200X User’s Manual LISST-200X Operation Details Page 68...
  • Page 77 Output: New Start Condition data = 2 Start Condition: Delay Start with 2 minute delay Set Stop Condition STOPCOND Syntax: SP x or STOPDATA x, where x is the stop condition code described below LISST-200X User’s Manual LISST-200X Operation Details Page 69...
  • Page 78 Stop Condition: Depth Stop at 3 meters input: PD 2016/12/31 23:59:59 (if Stop Condition = 2 (Time/Date Stop)) output: New Stop Condition data = 2016/12/31 23:59:59 Stop Condition: Time Stop at 2016/12/31 23:59:59 LISST-200X User’s Manual LISST-200X Operation Details Page 70...
  • Page 79 If no value follows command, prompts will be displayed for the value. Example: input: MA 32 output: New Measurements per Average: 32 Set Burst Interval Syntax: BI x, where x is the number of seconds between the start of 2 LISST-200X User’s Manual LISST-200X Operation Details Page 71...
  • Page 80 Example: input: SC 2017/01/05 21:05:03 output: Command Data in SC is: 2017/01/05 21:05:03 Current Date/Time: 2017/01/05 21:05:03 Enable Autostart Setting Syntax: AS x, where x is 1 (yes) or 0 (no) LISST-200X User’s Manual LISST-200X Operation Details Page 72...
  • Page 81 Issue a DS command to verify the status of the store mode setting. A warning will be displayed as part of the DS status if the store mode is disabled: WARNING: Data storage disabled. Data will not be saved!!! LISST-200X User’s Manual LISST-200X Operation Details Page 73...
  • Page 82 Do you wish to reset Depth Sensor offset? (1=yes,0=no): [0] ? 1 Previous offset was -14.03. New offset is -13.87. Previous Depth was -0.15 meters. New Depth using corrected offset is 0.00 meters. Cautions: None LISST-200X User’s Manual LISST-200X Operation Details Page 74...

  • Page 83: Acquisition/Action Commands

    CTRL-C. Grab sample and transmit it Syntax: Description: Acquires single averaged sample and displays the result to the screen. Example: Input: GX Output: { …. 36 ring values + 24 Aux parameters LISST-200X User’s Manual LISST-200X Operation Details Page 75...
  • Page 84 0, with data transmission turned off. Setting 1 sends raw data in a multi-line text format; 2 sends a single line as a NMEA- compatible data sentence; 3 sends a single line of comma-separated values. Contact Sequoia Scientific for complete details of these formats. Example: input: XR 1...
  • Page 85 Description: Sends LISST-200X in to low power sleep mode. Instrument will send a message every 30 seconds to indicate it is sleeping. It can be wakened at any time by sending 2 control-C characters about ½ second apart. Example: Input: ZZ Output: Deep Sleep…...

  • Page 86: Performance Optimization

    All of the other possibilities must be eliminated before it can be concluded that the instrument is misaligned. The transmit optics on the LISST-200X do allow for the possibility of the user adjusting the alignment. A written procedure can be provided for this procedure.
  • Page 87 The low concentration limit of LISST-200X is very sensitive to the quality of the background scatter file. For this reason, when working in low concentration water, it is very important that a good background file be obtained with very clean water.
  • Page 88 Contact Sequoia Scientific for assistance. ALR works by measuring the signal on the LISST-200X’s 36 detectors first with the laser on, then with the laser off. This on-off process is repeated 3 times per second. Because of the time required for the laser and electronics to change between the on and off states, rapidly changing ambient light may not be completely rejected.

  • Page 89: Instrument Mounting, Deployment Orientation, Storage And Maintenance

    Instrument Mounting, Deployment Orientation, Storage and Maintenance This section contains information on mounting, storing and maintaining the LISST-200X. Horizontal The LISST-200X measures scattered light to obtain particle size Mounting distributions. Therefore it is important to keep particles from sticking to the window surface. The first line of defense it to keep the window surface vertical by mounting the instrument horizontally.
  • Page 90 Any contact with other metal can greatly increase the rate of corrosion. Isolate the instrument with rubber or plastic to keep the LISST-200X from being the sacrificial anode for the mounting hardware. Failure to properly isolate the instrument from all other metal will void the warranty.
  • Page 91 Calibrations and The LISST-200X should not require any adjustment or calibrations. Adjustments The pressure and temperature sensors can be re-calibrated if desired.

  • Page 92: Appendix A: Technical Specifications

    • Input power: o Operating range: 9 to 24 V o Current during active sampling: ▪ LISST-200X: 100 mA @ 12V ▪ LISST-Black: 220 mA @ 12V ▪ LISST-HAB: 180 mA @ 12V o Current while waiting for user command: < 50 mA @ 12V o Current during sleep: <...
  • Page 93 LISST-200X User’s Manual Appendix A: Technical Specifications Page 85...

  • Page 94: Appendix B: Particle Size Bins

    20.9 19.2 20.9 24.6 22.7 24.6 29.1 26.7 29.1 34.3 31.6 34.3 40.5 37.2 40.5 47.7 43.9 47.7 56.3 51.9 56.3 66.5 61.2 66.5 78.4 72.2 78.4 92.6 85.2 92.6 LISST-200X User’s Manual Appendix B: Particle Size Bins Page 86...

  • Page 95: Appendix C: Data File Formats

    Appendix C: Data File Formats File Types and Extensions Used by the LISST-200X Software Extension Discription Format Comments .RBN Raw Data Binary Raw data file produced by the LISST-200X. .RTX Raw Data ASCII Comma delimited ASCII representation of .RBN file...
  • Page 96 Raw pressure [least significant 16 bits] Ambient Light [counts – not calibrated] External analog input 3 [V] (fluorometer 3 in LISST-HAB & LISST-Black) Computed optical transmission over path [dimensionless] Beam-attenuation (c) [m LISST-200X User’s Manual Appendix C: Data File Formats Page 88...
  • Page 97 Accelerometer Z [counts] [not presently used] Raw pressure [most significant bit] Raw pressure [least significant 16 bits] Ambient light [counts] External Analog input 3 [10 V/count] (fluorometer 3 in LISST-HAB & LISST- Black) LISST-200X User’s Manual Appendix C: Data File Formats Page 89...
  • Page 98 LISST-200X User’s Manual Appendix C: Data File Formats Page 90...

  • Page 99: Appendix D: Processing Data Files Using Matlab

    Two Matlab functions for processing data are provided on the instrument USB card. They are also available from the ‘Software and Downloads’ tab on the LISST-200X webpage. The processing is split into two steps: (1) reading in and applying corrections to raw data files (.RBN) and (2) inverting the corrected scattering to a particle size distribution.
  • Page 100 ShowProgressBar – If set to 1, a progress bar will display the processing status. The function will return the volume distribution in uL/L (vd) and the midpoint of each size bin in microns (dias). LISST-200X User’s ManualAppendix D: Processing Data Files Using MATLAB Page 92...

  • Page 101: Appendix E: Connectors

    Appendix E: Connectors IMPORTANT NOTE In September 2019, starting with serial number 2116, LISST-200X instruments and cables use connectors manufactured by SubConn. Previous instruments have connectors from Teledyne Impulse. Connectors from the two manufacturers are very similar, but are not guaranteed to be compatible.
  • Page 102 The LISST-200X has 3 separate underwater connectors: A 5-pin, a 6-pin, and a 3-pin connector. The photograph shows the placement of each connector. The following text describes the detailed wiring for each connector. Note that in new instruments starting in September 2019, these are SubConn connectors with brass bodies.
  • Page 103 Serial Out (to DB-9 Pin 2) Serial In (to DB-9 Pin 3) BioBlock Connector (3 pin connector) Bulkhead connector: SubConn MCBH3M Mating Cable Part Number: SubConn MCIL3F Connector Pin # Signal Ground Signal (Open/Closed) Battery Sense LISST-200X User’s Manual Appendix E: Connectors Page 95...
  • Page 104 In earlier versions, this pin is not connected. Analog Output Configuration Connector Pin # Ground (Power) Analog Output: Mean Size Ground (signal) Analog Output: Total Concentration Ground (signal) Power In (8 to 24VDC) LISST-200X User’s Manual Appendix E: Connectors Page 96...
  • Page 105 Serial In (to DB-9 Pin 3) To PC Connector ( 5-pin Male Bulkhead) Connector Pin # No Connection No Connection Ground Serial Out (to DB-9 Pin 2) Serial In (to DB-9 Pin 3) LISST-200X User’s Manual Appendix E: Connectors Page 97...

  • Page 106: Appendix G: Spherical And Random Particle Shape Models

    We briefly comment on shape effects here. Particles of different shapes scatter light differently. Two models are offered by Sequoia – the spherical model, and a random shape model. The resulting inversion of data will differ slightly for the two models.

  • Page 107: Appendix H: Lisst-Black & Lisst-Hab

    (LISST-Black), or harmful algal blooms (LISST-HAB). The fluorometers are Turner Designs Cyclops-7F’s, mounted with special brackets and cabling made by Sequoia Scientific. The LISST-200X supplies power to the fluorometers, and digitizes their analog outputs. The digitized fluorometer outputs are included in each data sample produced by the LISST, at rates up to one per second.
  • Page 108 9/64-inch hex L-key #2 Phillips Screwdriver 1. Attach the Front Support to the LISST-200X with the Clamp Strap, cross nut and clamping screw. Position the tab on the Front Support so it fits flush against the endcap and aligns to the flat surface. Tighten the Clamp Screw.
  • Page 109 4. Rotate the Mid Support as needed to make the axis of the sensors parallel to the axis of the LISST-200X. Tighten the Mid Support clamp making sure that the Mid Support is pushed against the tapered part of the pressure case, as in step 2. The sensors should be free to rotate and move forward and backward.

  • Page 110: Appendix I: Technical Assistance & Troubleshooting

    A: In some cases the offload baud rate may need to lowered in order avoid offload errors. The offload baud rate can be reduced in the LISST-200X software selecting a lower baud rate from the drop down box on the file offload page: Select a lower File Offload Baud Rate, then try transfering files again.
  • Page 111 If you purchased the instrument directly from Sequoia, email the data to info@SequoiaSci.com. PLEASE NOTE: We cannot use the .PBN or .RTX files produced by the LISST-200X software for troubleshooting. We can only help you if you supply .RBN files.

  • Page 112: Warranty

    If, during the twelve months after delivery (the “Warranty Period”), any Product does not comply with the warranties set forth in 1(a) and 1(b) above, SEQUOIA will, at its option, either (a) repair the Product, (b) replace the Product, or (c) refund the purchase price paid by Customer to SEQUOIA for the Product;...
  • Page 113 Product (including, but not limited to, any breach of or default by SEQUOIA) will in no event exceed the purchase price paid by Customer to SEQUOIA for such Product. Further, SEQUOIA will not be liable for, or be in breach of or default on account of, any delay or failure to perform as a result of any cause, condition or circumstance beyond SEQUOIA's reasonable control.
  • Page 114 Software clause at 48 C.F.R. § 252.227-7013 or in subparagraph (c)(2) of the Commercial Computer Software-Restricted Rights clause at 48 C.F.R. § 52.227-19, as applicable. Portions of these items may be unpublished. SEQUOIA reserves all rights under applicable copyright laws. 12. Controlling Document...

  • Page 115: Revision History

    Revision History Version 2.0 Updated descriptions and screenshots for new LISST-200X software. Version 1.5A Corrections in Appendix H. Version 1.5 Add LISST-HAB and LISST-Black Version 1.4 Show change from Impulse to SubConn connectors. Version 1.3B Note that accelerometer data are not presently calibrated or used (they are reserved for possible future use) Version 1.3A Edit XR command description.

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