1. Manuals
  2. Brands
  3. Sequoia Manuals
  4. Measuring Instruments
  5. LISST-200X
  6. User manual

Sequoia LISST-200X User Manual

Particle size analyzer
Hide thumbs Also See for LISST-200X:
Table of Contents

Advertisement

Quick Links

Download this manual
LISST-200X
Particle Size Analyzer
User's Manual
Version 1.3B
(January 2018)
Store Software
USB Card Here
2700 Richards Road, Suite 107
Bellevue, WA 98005-4200
Tel: (425) 641-0944 Fax: (425) 643-0595

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the LISST-200X and is the answer not in the manual?

Questions and answers

Related Manuals for Sequoia LISST-200X

Summary of Contents for Sequoia LISST-200X

  • Page 1 LISST-200X Particle Size Analyzer User’s Manual Version 1.3B (January 2018) Store Software USB Card Here 2700 Richards Road, Suite 107 Bellevue, WA 98005-4200 Tel: (425) 641-0944 Fax: (425) 643-0595...
  • Page 3  This document is copyrighted by SEQUOIA SCIENTFIC, INC LISST-200X User’s Manual Page iii...
  • Page 5 This manual is divided into two sections. I. LISST-200X contains an introduction to the LISST-200X instrument and the principles of its operation. II. LISST-200X Operation provides a detailed set of instructions for using and caring for the instrument. Technical assistance For technical assistance please contact your local Distributor or Sequoia.

  • Page 7: Table Of Contents

    View Processed Data File ..................61 Data Quality Control ....................64 Simple Real-Time Data Processing ................. 66 Collecting Data from an External Analog Sensor ............. 69 Configuring the LISST-200X as a Sensor for a CTD ..........70 ..................... 71 NSTRUMENT OMMUNICATION LISST-200X C ..................
  • Page 8 LISST-200X User’s Manual Table of Contents Page viii...

  • Page 9: Lisst-200X Introduction And Tutorial

    (PSD). That is the essence of this method. In order that you appreciate the importance of some steps in operation of the LISST-200X, we recommend reading the rest of this chapter. The measurement of scattering at multiple angles is done with a collimated beam illuminating particles in water.
  • Page 10 A factory background acts as a reference to compare with, to identify degradation in laser output or fouling of optical surfaces. The LISST-200X stores its factory background data in its memory, as well as the user’s acquired background data.
  • Page 11 As to which particle model to use, we suggest that when working with natural waters, use the irregularly shaped model. Only in exceptional circumstances, the spherical model is appropriate. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 3...

  • Page 12: Lisst-200X General Description

    Windows software. For MATLAB users, inversion scripts are available for download from Sequoia’s website: www.SequoiaSci.com. The LISST-200X instrument is a laser diffraction device. It consists of optics for producing a collimated laser beam, a specially constructed detector array, electronics for signal pre-amplification and processing, data storage and scheduling computer, and an external battery system.
  • Page 13 A gauge pressure sensor is calibrated to measure the pressure relative to a given atmospheric pressure, as opposed to an absolute pressure sensor, which measures pressure relative to a vacuum. The pressure sensor on the LISST-200X will read 0 at atmospheric pressure.
  • Page 14 Battery Life The LISST-200X does not contain internal batteries (except a small battery to maintain the real-time clock), but can be powered from multiple sources including a USB computer cable, Small or Large Battery Packs (provided with the instrument) or an external instrument such as a CTD.

  • Page 15: Lisst-200X Quick Start Tutorial

    User’s manual following this Quick Start Tutorial. Let’s assume that you are opening the LISST-200X shipping case for Contents of Shipping Case the first time.
  • Page 16 Instrument from a flat working surface. Remove the LISST-200X from the case and set Shipping Case. it on the stands. The LISST-200X has two distinct ends that we will refer to as the Optics endcap and the Connector endcap. Optics Endcap The optics endcap contains the optical windows that the laser beam passes through to make a measurement.
  • Page 17 This magnet can be used to trigger a digital switch inside the instrument which can be programmed to start and stop the LISST-200X sampling. The final item on the endcap is the zinc anode. The sacrificial zinc anode protects the instrument from corrosion during long periods of time in salt water.
  • Page 18 If the driver installed correctly, you should see the green light on the USB cable blinking. For more information on establishing and troubleshooting communication with the LISST-200X see the Step-by-Step guide on page 32 of the User’s Manual.
  • Page 19 We can now fill the chamber with clean, particle-free water. Sequoia uses steam-distilled bottled water filtered through a 0.2 micron filter. Tap water may contain too many particles and may also contain dissolved gas that can release and form small bubbles on the optical surfaces.
  • Page 20 Communication menu select Serial Port Settings. You will see the window below: Select the port your LISST-200X is connected to from the drop down menu of next to ‘Serial Port.’ The other two fields can be left at their default values. Press the ‘OK’ button to save the changes.
  • Page 21 The STOP button sends a CTRL-C to stop the instrument from its current process. A list of the commands that can be sent to the LISST-200X can be found in the section LISST-200X Command Summary on page 75.
  • Page 22 ‘Background Help’ button will open a PDF with additional information about background measurements. When the BEGIN Collect button is pressed 20 samples will be displayed to the screen as they are acquired. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 14...
  • Page 23 20 measurements. If the background is close to factory levels the message displayed will indicate a pass or acceptable background. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 15...
  • Page 24 If the values are acceptable the values can be saved both onboard the instrument and to a file on your computer. The LISST-200X handles the background files differently than the LISST-100X. The background file is stored on the instrument and is saved as part of every data file that is recorded.
  • Page 25 This screen is used to set the type of sampling; Fixed Sample Rate or Burst. You can also select the samples per average and sample rates on this screen. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 17...
  • Page 26 0.53 seconds. AutoStart If the AutoStart check box is selected, the LISST-200X will start the sampling program when power is applied to the instrument. If this is not selected the user must send the ‘GO’ command to start the sampling programing after apply power.
  • Page 27 Then return to the Instrument Status page by clicking on the Instrument Status Tab. It may take several seconds to configure the sampling parameters on the LISST-200X. The status bar at the bottom of the main window will indicate when a process is underway: The status bar will return to ‘Ready’...
  • Page 28 For example, if you have selected the mechanical switch as the start condition, the instrument will only react to the swtich after the sampling program has been started. Only then will actual LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 20...
  • Page 29 Data collection will continue until the Stop Conditions are met. In our case, until the switch lever is returned to the “0” position. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 21...
  • Page 30 Choose the files to offload by clicking on them while holding down the CTRL key. The Shift key can also be used to select a range of files. Select OK and then choose a location to save the files to. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 22...
  • Page 31 .RBN, and are offloaded directly from the LISST- 200X data logger. Below is list of the file types you can expect to see when using the SOP software. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 23...
  • Page 32 .PBN file is always created. Output of other file types (.CSV or .RTX) depends on the selections in the Settings selection in the File menu. Every LISST-200X data file contains all the necessary information to process the file. Therefore, the software will automatically determine the instrument serial number, factory background, current background and other instrument specific parameters.
  • Page 33 Use the First button to return to the first sample and press the Stop Display button to restart the display. To view the value of the auxiliary parameters, such as depth and LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 25...
  • Page 34 To open the Real-Time session, choose Collect Realtime Data from the LISST menu or press the button. Choose an output PBN file. A display very similar to viewing a processed PBN file will be displayed. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 26...
  • Page 35 The raw scattering values on the detector rings can also be viewed by selecting View Rings from the View menu. A typical display is shown below. LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 27...
  • Page 36 The above steps should have given you a good understanding on the LISST-200X in how to operate the LISST-200X. More details on all of the steps can be found in the User’s Manual following this tutorial. The following the Field steps go into deploying the instrument in the field.
  • Page 37 Window. When AutoStart is enabled a message will be displayed as part of the Status command or the Query instrument results. When the LISST-200X is powered up a message will be displayed to the Terminal window (or via the RS232 interface) that will prompt the user that the instrument will auto start in 5 seconds.
  • Page 38 LISST-200X User’s Manual LISST-200X Introduction and Tutorial Page 30...

  • Page 39: Lisst-200X Operation Details

    WARNING The LISST-200X uses a laser diode emitting a maximum of 1 mW of visible (red) light at a wavelength of 670nm. The laser beam under normal circumstances is not a threat. However, if objects are placed in the path of the laser beam, the light could be reflected into the eye causing permanent damage.

  • Page 40: Step By Step Procedures

    D. Step by Step Procedures Installing LISST-SOP Software Software for the PC is used to configure the LISST-200X and for downloading and processing the size distributions. STEP ACTION RESULT • The LISST-200X comes with a USB memory card. Installer was found on...

  • Page 41: Establishing Communication With The Lisst-200X

    Establishing Communication with the LISST-200X Establish communication with LISST-200X using the supplied software and USB cable. Communicating with the LISST-200X via the LISST-SOP software should be automatic. Should there be a problem you can use these step-by-step instructions to troubleshoot the problem.
  • Page 42 • After connecting, may need to wake your instrument LISST-200X is awake and ready to configured from sleep before continuing. To wake up the or collect data instrument select Wake Up LISST from the LISST menu or press the button. A dialog box will appear counting down the maximum time required for the instrument to wake up.

  • Page 43: Saving And Evaluating Clean Water Backgrounds

    LISST-SOP open and communicating with communication in the LISST-SOP200X program. (see LISST-200X. Step-by-Step Procedure: Establishing Communication with the LISST-200X) • Clean instrument and install Clean Water Background Optics submerged in water Test chamber. • Fill with clean filtered water and make sure no bubbles are in the water or on the windows.
  • Page 44 If needed you can update the background, such as after cleaning the windows or replacing the water, by pressing the BEGIN Collect button again. LISST-200X User’s Manual LISST-200X Operation Details Page 36...
  • Page 45 ‘Accept and Save’ button. It is not necessary to store the background on your computer, however, these saved backgrounds can be used during processing instead of the background saved in the data file. LISST-200X User’s Manual LISST-200X Operation Details Page 37...

  • Page 46: Configuring Data Collection

    Operating Modes from the LISST menu or by pressing the button on the toolbar. Clock set • To set the LISST-200X data logger clock to the computer clock press the Set Clock button. Press the Refresh Status button to refresh the display.
  • Page 47 If this is not selected the user must send the ‘GO’ command to start the sampling programing after apply power. • NOTE: Auto Start will not occur if the power is connected to LISST-200X in low power sleep mode. LISST-200X User’s Manual LISST-200X Operation Details...
  • Page 48 • The available stop conditions are: Depth, Time. External Mechanical Switch, External Digital Input, Fixed number of samples, and Memory Full. • If the Stop Condition is Depth, External Mechanical Switch, LISST-200X User’s Manual LISST-200X Operation Details Page 40...
  • Page 49 PRM installed. If you are deploying the instrument with the PRM installed, the clean water background must also be collected using the PRM. LISST-200X User’s Manual LISST-200X Operation Details Page 41...
  • Page 50 We recommend selecting to open the terminal window, so you can confirm the sampling program has been started. • If you select the Cancel button, the message below appears, informing you how you can start the instrument. LISST-200X User’s Manual LISST-200X Operation Details Page 42...
  • Page 51 Once the program is confirmed to be running and waiting for the correct start conditions, the LISST-SOP program can be closed and the communications cable can be disconnected. Be sure to replace the connector cap before deployment. LISST-200X User’s Manual LISST-200X Operation Details Page 43...

  • Page 52: Using The Small External Battery Pack

    Using the Small External Battery Pack The LISST-200X comes with a Small External Battery Pack that can be used for short term deployments. The instructions in this section describe how to use the Small Battery including installing and charging the NiMH rechargeable batteries.
  • Page 53 Align the thumbscrews with the holes in the main housing and push the endcap on until it is flush with the housing. • Tighten the thumbscrews by hand (DO NOT USE ANY TOOL). DO NOT OVERTIGHTEN. LISST-200X User’s Manual LISST-200X Operation Details Page 45...
  • Page 54 Bulkhead connector on the Small Battery Pack. • Connect the other end of the cable to the 5-pin Male bulkhead on the LISST-200X. • Power is now going to the LISST-200X. If AutoStart is enabled the instrument may be looking for the start condition.

  • Page 55: Using The Large External Battery Pack

    Using the Large External Battery Pack The Large External Battery Pack is used to provide power to the LISST-200X for long term deployments or extended profiling operations. STEP ACTION RESULT • The LISST-200X Large External Battery is shipped Battery ready to use when shipped.
  • Page 56 • Screw the connector endcap onto the pressure case Battery assembled and ready for use. with the four 6-32 x ¾ socket head cap screws. If necessary, apply anti-seize compound onto the threads. LISST-200X User’s Manual LISST-200X Operation Details Page 48...
  • Page 57 “TO LISST”. • Connect the other end of the cable to the 5-pin Male bulkhead on the LISST-200X. • Power is now going to the LISST-200X. If AutoStart is enabled the instrument may be looking for the start condition.
  • Page 58 • If desired connect the Communications cable to the male 5-pin bulkhead connector on the Large Battery Pack which is labeled “TO PC”. Communications will pass through the battery to the LISST-200X. • The battery and communications cables are shown below.

  • Page 59: Offloading And Deleting Data Files From Internal Memory

    Offloading and Deleting Data Files from Internal Memory Data collected using the LISST-200X is stored on the internal data logger. This section describes how to offload and delete files from the internal data logger. STEP ACTION RESULT • .LISST-SOP open and...

  • Page 60: Processing A Single Raw Data File

    Excel etc. • The RTX file is a space delimited ASCII file containing the raw data from the LISST-200X data logger. This is useful for looking at the raw data in a spreadsheet such as Excel etc.
  • Page 61 • Note: file types other than the default PBN file must have been selected before selecting Process Raw Data from the File menu (See step 2). LISST-200X User’s Manual LISST-200X Operation Details Page 53...
  • Page 62 You can click on the lower plot to move the cursor to a new location. An example of this window is shown below. LISST-200X User’s Manual LISST-200X Operation Details Page 54...
  • Page 63 Pressing the Select First button will set the current cursor position as the starting point for processing. Similarly selecting Select Last will set the LISST-200X User’s Manual LISST-200X Operation Details Page 55...
  • Page 64 Processing Completed. File button. The display will show the processing progress and the Process File button text will change to Finished when the processing is complete. The window can now be closed. LISST-200X User’s Manual LISST-200X Operation Details Page 56...

  • Page 65: Batch Processing Multiple Raw Data Files

    • The CSV file is a space delimited ASCII file containing all the processed data. • The RTX file is a space delimited ASCII file containing the raw data from the LISST-200X data logger. • You must also select the inversion model to be used Select inversion method(s) to be used –...
  • Page 66 If you would like to use an external .BGT file, uncheck the ‘Use background contained in files’ and select a .BGT file. LISST-200X User’s Manual LISST-200X Operation Details Page 58...
  • Page 67 Particle Inversion Model, the processed files will have an’ _rs’ suffix. • Note: file types other than the default PBN file must have been selected before starting the batch processing. See step 2. LISST-200X User’s Manual LISST-200X Operation Details Page 59...
  • Page 68 Output Files window. Note that the files processed using the randomly shaped inversion method have an ‘_rs’ suffix. • Click the close button in the bottom right when you are Close the batch processing window. done. LISST-200X User’s Manual LISST-200X Operation Details Page 60...

  • Page 69: View Processed Data File

    The Start Display button will step through the data file by updating the display at a fixed rate. The rate is set by the slider bar next to the Start Display button. LISST-200X User’s Manual LISST-200X Operation Details Page 61...
  • Page 70 These features are located on the Display tab of the Settings window as shown below. LISST-200X User’s Manual LISST-200X Operation Details Page 62...
  • Page 71 • The current display can be sent to the printer by Display printed. choosing Print from the File menu. • Print Preview and Print Setup are also available. • When finished viewing the processed data close the Viewing of data complete. window. LISST-200X User’s Manual LISST-200X Operation Details Page 63...

  • Page 72: Data Quality Control

    In this case only your pressure data and temperature data will be any good. The laser must be replaced. Contact Sequoia or your local distributor for instructions on what to do. • Tip: If you selected ‘Create .CSV file’ when processing your data, you can load the .CSV file...
  • Page 73 Quick quide for data • Disregard data if laser reference is 0 or very close to 0 (laser is dead, contact Sequoia). • Disregard data if transmission is > 0.995 (> 99.5%). • Disregard data if transmission is < 0.10 (< 10%).

  • Page 74: Simple Real-Time Data Processing

    • Connect the instrument to the computer and establish Communication established. communication in the LISST-SOP200X program. (see Step-by-Step Procedure: Establishing Communication with the LISST-200X) • Before starting a Real-Time session the output file options Output file type selected. must be set. This must be done before selecting Collect Realtime Data.
  • Page 75 .CSV file. If you selected both particle models in part 2, data from both models will be saved to your computer regardless of the radio button selection. LISST-200X User’s Manual LISST-200X Operation Details Page 67...
  • Page 76 Averaged data acquired and stored pressing the Save Single Sample button to an ASCII file. • Press the ‘Stop Collection’ button and close the window to Stop data collection end the realtime session. LISST-200X User’s Manual LISST-200X Operation Details Page 68...

  • Page 77: Collecting Data From An External Analog Sensor

    If using the 12V output, you can specify how long it should turn on before sampling, as a warmup time for the sensor. To provide these functions, the auxiliary connector on the LISST-200X must be in one of two configurations: Analog & Digital Input (which provides a single analog input), or Dual Analog Input configuration (which provides two).

  • Page 78: Configuring The Lisst-200X As A Sensor For A Ctd

    For highest-quality data you will still need to offload and process the detailed data from the LISST-200X after deployment. The LISST-200X updates its analog outputs each time it collects a sample, at a maximum of 1 Hz.

  • Page 79: Instrument Communication

    Instrument Communication Overview The cables Sequoia supplies with the LISST-200X send data through a standard USB port. However, the LISST-200X itself communicates through RS-232 serial protocol. The 5-pin male connector on the LISST-200X carries the RS-232 signals (and power). The cables incorporate adapters that convert between RS-232 and USB.
  • Page 80 The details of each condition are described below. Depth Start The built-in depth sensor of the LISST-200X is used to check the current depth to determine if the desired start depth has been exceeded. The instrument is powered up and 5 measurements of the depth are averaged over a two-second period.
  • Page 81 Depth Stop The depth sensor of the LISST-200X is used to check the current depth to determine if it is less than the desired Stop depth. If the depth is less than the threshold the sampling will stop. If the Start Condition is Depth Start the program will return to checking for the start depth.
  • Page 82 Memory Full Sampling will continue until the memory is full, or the power input falls. If the memory fills, the instrument will go into deep sleep. LISST-200X User’s Manual LISST-200X Operation Details Page 74...

  • Page 83: Lisst-200X Command Summary

    LISST-200X Command Summary NOTE: Command are shown in upper case for clarity, but are not case-sensitive. Display Commands CONFIG Display instrument hardware configuration information DD, DIR Display current disk directory DEPTH Display current depth Display current instrument status information Display current time and date...
  • Page 84 Start data collection using current parameters Grab sample and transmit it Transmit raw data when average saved Acquire 20 samples and evaluate them for quality. Go into deep sleep mode (minimum power consumption) LISST-200X User’s Manual LISST-200X Operation Details Page 76...

  • Page 85: Lisst-200X Command Details

    G. LISST-200X Command Details Display Commands CONFIG Display instrument hardware configuration Syntax: CONFIG Description: The current hardware configuration including Auxiliary I/O connector configuration Example: input: CONFIG output: Serial Number: 2001 Firmware Version: 1100 (1.100) VCC: 59371 Full Path Length: 2500 (25 mm) Eff.
  • Page 86 Description: The instrument settings and status are displayed to the screen. Example: input: DS output: LISST-200X Status and Settings Serial number = 1004 Endcap Configuration: 0 (Analog & digital in) Firmware Version 1.126 Apr 26 2016 18:45:52 LISST-200X User’s Manual...
  • Page 87 External sensor power during logging: off External sensor warmup time: 0 seconds Free memory: 1023 Mbytes input: DS 1 output: LISST-200X Current Status and Settings SN = 1004 OM = 0 ST = 5 TD = 0 SP = 5...
  • Page 88 Description: Displays the list of command to the screen. Example: input: HE output: LISST-200X Commands (followed by 30 lines of text) Display current Mechanical Switch position Syntax: Description: The current position of the Mechanical Switch is displayed to the screen.

  • Page 89: Setup Commands

    If no value follows command, prompts will be displayed for the value. After setting the start condition to Depth, Time or Delay, use STARTDATA to set the applicable condition value. Example: input: ST 5 output: New Start Condition Setting: Delay Start LISST-200X User’s Manual LISST-200X Operation Details Page 81...
  • Page 90 Set Stop Condition STOPCOND Syntax: SP x or STOPDATA x, where x is the stop condition code described below Description: The SP command sets the stop condition to be used when collecting data. LISST-200X User’s Manual LISST-200X Operation Details Page 82...
  • Page 91 Stop Condition: Depth Stop at 3 meters input: PD 2016/12/31 23:59:59 (if Stop Condition = 2 (Time/Date Stop)) output: New Stop Condition data = 2016/12/31 23:59:59 Stop Condition: Time Stop at 2016/12/31 23:59:59 LISST-200X User’s Manual LISST-200X Operation Details Page 83...
  • Page 92 If no value follows command, prompts will be displayed for the value. Example: input: MA 32 output: New Measurements per Average: 32 Set Burst Interval Syntax: BI x, where x is the number of seconds between the start of 2 LISST-200X User’s Manual LISST-200X Operation Details Page 84...
  • Page 93 Command Data in SC is: 2017/01/05 21:05:03 Current Date/Time: 2017/01/05 21:05:03 Enable Autostart Setting Syntax: AS x, where x is 1 (yes) or 0 (no) Description: With Autostart enabled, the firmware will immediately start the sampling LISST-200X User’s Manual LISST-200X Operation Details Page 85...
  • Page 94 Issue a DS command to verify the status of the store mode setting. A warning will be displayed as part of the DS status if the store mode is disabled: WARNING: Data storage disabled. Data will not be saved!!! LISST-200X User’s Manual LISST-200X Operation Details Page 86...
  • Page 95 Do you wish to reset Depth Sensor offset? (1=yes,0=no): [0] ? 1 Previous offset was -14.03. New offset is -13.87. Previous Depth was -0.15 meters. New Depth using corrected offset is 0.00 meters. Cautions: None LISST-200X User’s Manual LISST-200X Operation Details Page 87...

  • Page 96: Acquisition/Action Commands

    CTRL-C. Grab sample and transmit it Syntax: Description: Acquires single averaged sample and displays the result to the screen. Example: Input: GX Output: { …. 36 ring values + 24 Aux parameters LISST-200X User’s Manual LISST-200X Operation Details Page 88...
  • Page 97 0, with data transmission turned off. Setting 1 sends raw data in a multi-line text format; 2 sends a single line as a NMEA- compatible data sentence; 3 sends a single line of comma-separated values. Contact Sequoia Scientific for complete details of these formats. Example: input: XR 1...
  • Page 98 Description: Sends LISST-200X in to low power sleep mode. Instrument will send a message every 30 seconds to indicate it is sleeping. It can be wakened at any time by sending 2 control-C characters about ½ second apart. Example: Input: ZZ Output: Deep Sleep…...

  • Page 99: Performance Optimization

    All of the other possibilities must be eliminated before it can be concluded that the instrument is misaligned. The transmit optics on the LISST-200X do allow for the possibility of the user adjusting the alignment. A written procedure can be provided for this procedure.
  • Page 100 The low concentration limit of LISST-200X is very sensitive to the quality of the background scatter file. For this reason, when working in low concentration water, it is very important that a good background file be obtained with very clean water.
  • Page 101 Contact Sequoia Scientific for assistance. ALR works by measuring the signal on the LISST-200X’s 36 detectors first with the laser on, then with the laser off. This on-off process is repeated 3 times per second. Because of the time required for the laser and electronics to change between the on and off states, rapidly changing ambient light may not be completely rejected.

  • Page 102: Instrument Mounting, Deployment Orientation, Storage And Maintenance

    Instrument Mounting, Deployment Orientation, Storage and Maintenance This section contains information on mounting, storing and maintaining the LISST-200X. Mount the The LISST-200X measures scattered light to obtain particle size Instrument distributions. Therefore it is important to keep particles from sticking Horizontal to the window surface.
  • Page 103 Any contact with other metal can greatly increase the rate of corrosion. Isolate the instrument with rubber or plastic to keep the LISST-200X from being the sacrificial anode for the mounting hardware. Failure to properly isolate the instrument from all other metal will void the warranty.
  • Page 104 Storage Notes Again it must be emphasized that the LISST-200X is a sensitive instrument. When not in use, the instrument should be stored in its shipping case. Cleaning the As has been noted earlier, the condition of the windows is critical to Optical Windows the performance of the LISST-200X.
  • Page 105 Comparison of the LISST-200X's performance with that of other particle-size measuring devices that are not based on laser diffraction may result in inconsistencies because the instruments may measure different properties of the particles to interpret particle size. LISST-200X User’s Manual...

  • Page 106: Appendix A: Technical Specifications

    • Dimensions: 10.3 cm (3.95") Æ x 63.9 cm (26.18") L • Weight in air: 5.4 kg (11.8 lbs.) • Weight in water: 1.7 kg (3.8 lbs.) • Depth rating: 600 m LISST-200X User’s Manual Appendix A: Technical Specifications Page 98...
  • Page 107 LISST-200X User’s Manual Appendix A: Technical Specifications Page 99...

  • Page 108: Appendix B: Particle Size Bins

    20.9 19.2 20.9 24.6 22.7 24.6 29.1 26.7 29.1 34.3 31.6 34.3 40.5 37.2 40.5 47.7 43.9 47.7 56.3 51.9 56.3 66.5 61.2 66.5 78.4 72.2 78.4 92.6 85.2 92.6 LISST-200X User’s Manual Appendix B: Particle Size Bins Page 100...

  • Page 109: Appendix C: Data File Formats

    (1 column, 59 rows) .LOP Operating Procedure ASCII File containing sampling parameters that can loaded onto an instrument in the LISST- SOP200X software LISST-200X User’s Manual Appendix C: Data File Formats Page 101...
  • Page 110 Raw pressure [most significant bit] Raw pressure [least significant 16 bits] Ambient Light [counts – not calibrated] Not used (set to zero) Computed optical transmission over path [dimensionless] Beam-attenuation (c) [m LISST-200X User’s Manual Appendix C: Data File Formats Page 102...
  • Page 111 Accelerometer X [counts] [not presently used] Accelerometer Y [counts] [not presently used] Accelerometer Z [counts] [not presently used] Raw pressure [most significant bit] Raw pressure [least significant 16 bits] Ambient light [counts] Not used LISST-200X User’s Manual Appendix C: Data File Formats Page 103...

  • Page 112: Appendix D: Processing Data Files Using Matlab

    Two Matlab functions for processing data are provided on the instrument USB card. They are also available from the ‘Software and Downloads’ tab on the LISST-200X webpage. The processing is split into two steps: (1) reading in and applying corrections to raw data files (.RBN) and (2) inverting the corrected scattering to a particle size distribution.
  • Page 113 ShowProgressBar – If set to 1, a progress bar will display the processing status. The function will return the volume distribution in uL/L (vd) and the midpoint of each size bin in microns (dias). LISST-200X User’s ManualAppendix D: Processing Data Files Using MATLAB Page 105...

  • Page 114: Appendix E: Connector Pinouts For Lisst-200X

    Appendix E: Connector Pinouts for LISST- 200X The LISST-200X has 3 separate underwater connectors: A 5-pin, a 6-pin, and a 3-pin connector. The photograph shows the placement of each connector. The following text describes the detailed wiring for each connector.
  • Page 115 Connector Manufacturer: Teledyne Impulse, Enterprise, Inc. San Diego, CA, USA Connector Part Number (Bulkhead): MCBH (WB)-36-MP Stainless Steel Mating Cable Part Number: MCIL-36-FS Connector Pin # Signal Ground Signal (Open/Closed) Battery Sense LISST-200X User’s Manual Appendix E: Connector Pinouts for LISST-200X Page 107...
  • Page 116 Analog Output: Total Concentration Ground (signal) Power In (8 to 24VDC) The current configuration of the Auxiliary I/O connector can be found by issuing a DS command at the command prompt. LISST-200X User’s Manual Appendix E: Connector Pinouts for LISST-200X Page 108...
  • Page 117 Serial In (to DB-9 Pin 3) To PC Connector ( 5-pin Male Bulkhead) Connector Pin # No Connection No Connection Ground Serial Out (to DB-9 Pin 2) Serial In (to DB-9 Pin 3) LISST-200X User’s Manual Appendix E: Connector Pinouts for LISST-200X Page 109...

  • Page 118: Appendix F: Lisst-Sop Software Button Description

    Pressing the button will toggle the communications status. Pressing the Open Terminal button will bring up the Open Terminal terminal window for communication with the instrument including Start and Stop buttons. LISST-200X User’s ManualAppendix F: LISST-SOP Software Button Description Page 110...
  • Page 119 Attempts to wake up instrument from low-power sleep Put LISST to sleep mode. This may take up to 40 seconds. Wake up LISST Puts instrument into low-power sleep mode. LISST-200X User’s ManualAppendix F: LISST-SOP Software Button Description Page 111...

  • Page 120: Appendix G: Spherical And Random Particle Shape Models

    We briefly comment on shape effects here. Particles of different shapes scatter light differently. Two models are offered by Sequoia – the spherical model, and a random shape model. The resulting inversion of data will differ slightly for the two models.

  • Page 121: Appendix H: Technical Assistance & Troubleshooting

    .RBN file(s) that contain the raw data. Email the data to your local Sequoia distributor, together with a detailed explanation of what you were doing and how the data were collected. If you purchased the instrument directly LISST-200X User’s ManualAppendix H: Technical Assistance &...
  • Page 122 Open up the terminal window and confirm that no program is running. If a program is running, stop it by clikcing the button, close the terminal window and try to query the instrument again. LISST-200X User’s ManualAppendix H: Technical Assistance & Troubleshooting Page 114...

  • Page 123: Warranty

    If, during the twelve months after delivery (the “Warranty Period”), any Product does not comply with the warranties set forth in 1(a) and 1(b) above, SEQUOIA will, at its option, either (a) repair the Product, (b) replace the Product, or (c) refund the purchase price paid by Customer to SEQUOIA for the Product;...
  • Page 124 Product (including, but not limited to, any breach of or default by SEQUOIA) will in no event exceed the purchase price paid by Customer to SEQUOIA for such Product. Further, SEQUOIA will not be liable for, or be in breach of or default on account of, any delay or failure to perform as a result of any cause, condition or circumstance beyond SEQUOIA's reasonable control.
  • Page 125 The Products may include or be delivered with certain computer programs, databases or other software that is proprietary to SEQUOIA. SEQUOIA hereby grants Customer a nonexclusive license to use such software solely for the purpose of operating Products. Customer will not: use any such software for any other purpose;...

  • Page 126: Revision History

    Version 1.2 Added support for path length reduction module (PRM) in LISST- SOP200X version 1.2. Extra fields added to .csv file, including ambient light. Added Appendix D: Processing Data Files Using MATLAB. Version 1.1 First released version. LISST-200X User’s Manual Revision History Page 118...

Table of Contents