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Optics
The CUV-TLC-50F provides ports for viewing or illuminating 1-cm square cuvettes from four directions.
Lenses are available to purchase separately to allow you to choose the best lenses for your application.
For absorbance and transmission measurements, position two CUV-TLC-CL Collimating Lenses at 180°.
For fluorescence applications, position two CUV-TLC-IL Imaging Lenses at 90° and position two CUV-
TLC-MP Mirror Plugs in the remaining two collimator positions to redirect energy to the sample or into a
lens.
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Collimating Lens—The Collimating Lens system, containing a single lens, collimates the fiber
output or focuses collimated light onto the end of another fiber. The collimating lens system is
typically used in absorption measurements.
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Imaging Lens—The Imaging Lens system, containing two lenses, focuses an image of the end
of the fiber into the cuvette, or light in the center of the cuvette onto another fiber (magnification
of approximately one). For added versatility, the second lens of the Imaging Lens system may be
removed so that it may be used as a Collimating Lens. The Imaging Lens system is typically used
in fluorescence measurements.
Typical Configuration for Absorbance Measurements
To measure Absorbance, a single collimating lens is placed on each side of the cuvette. In the following
figure, lenses and cuvette are shown in blue. Note that each lens holder has an alignment adjustment as
well as focusing capability.
CUV-TLC-50F Typical Configuration for Absorbance Measurements
000-30000-000-02-0205
Chapter 2
Operation
7
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